Detection of Arah1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensor

A gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in the development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection in food samples. Gold nanoparticles (average diameter=32 nm) were electrochemically generated on the surface of screen-printed carbon electrodes. Two monoclonal antibodies were used in a sandwich-type immunoassay and the antibody–antigen interaction was electrochemically detected through stripping analysis of enzymatically (using alkaline phosphatase) deposited silver. The total time of the optimized immunoassay was 3 h 50 min. The developed immunosensor allowed the quantification of Ara h 1 between 12.6 and 2000 ng/ml, with a limit of detection of 3.8 ng/ml, and provided precise (RSD <8.7%) and accurate (recovery >96.6%) results. The immunosensor was successfully applied to the analysis of complex food matrices (cookies and chocolate), being able to detect Ara h 1 in samples containing 0.1% of peanut.

A private library in the Mateus Palace (Vila Real). The D. Luís António de Sousa Botelho Mourão (1722-1798) Books Collection

Fundação para a Ciência e Tecnologia no âmbito de Bolsa de Doutoramento (SFRH/BD/86280/2012)

Publication Statistics Books 2000-

Vapaakappalekartuntaan perustuva tilasto Suomessa kustannetusta kirjallisuudesta vuodesta 2000 lähtien

L'expérience et approbation, Ulrich de Hutem,... : touchant la médecine du boys dit guaiacii pour circonvenir et déchasser la maladie de Neaples ([Reprod.]) / traduicte et interprétée par maistre Jehan Cheradame,...

Collection : French books before 1601 ; 413.3

Breviarium totius juris canonici ([Reprod.]) / Paulus Florentinus

Collection : French books before 1601 ; 134.2

Sermones praedicabiles ([Reprod.]) / Paulus Florentinus

Collection : Italian books before 1601 ; 528.1

Primum acconensis, deinde Tusculani episcopi : libri duo quorum prior orientalis, sive hierosolymitanae, alter occidentalis alter historiae nomine inscribitur ([Reprod.])

[Historia Hierosolymitana abbreviata (latin). 1597]

Llibri duo, quorum prior orientalis, sive hierosolymitanae, alter occidentalis historiae nomine inscribitur ([Reprod.]) / omnia nunc primum studio & opera D. Francisci Moschi,... e tenebris & situ in lucem edita

[Historia Hierosolymitana abbreviata (latin). 1597]

Divi Bernardi Abbatis clarevallis doctoris divertissimi ac vere melliflui opera omnia : divine institutionis plenissima, artificibus typis in unu coacta codicem sermones varii argumenti in sacre scripture locos principios ; Homilie in cantica canticorum ; Epistole tum familiares tum doctrinales... ([Reprod.])

Collection : French books before 1601 ; 37.1

Naturalis historie libri XXXVII : nuper studiose recogniti ([Reprod.]) / atque impressi adiectis variis Antonii Sabellici, Raphaelis Volaterrani, Beroaldi, [et al.]... adnotationibus ; ed. Nicolaus Beraldus

[Histoire naturelle (latin). 1516]

Nicolai Beraldi de vetere ac novitia jurisprudentia oratio : cum erudita ad antiquorum lectionem de studium exhortate ([Reprod.])

Collection : French books before 1601 ; 107.8

Casus longi super decretates ([Reprod.]) / Bernardus Parmensis

Collection : French books before 1601 ; 233.3

Compilatio Decretalium Gregorii IX [cum glossa Bernardi Bottoni. Edita a Francisco de Monelia]

Collection : Italian books before 1601 ; 214.1

Les amours de Tristan ([Reprod.]) / [Tristan L'Hermite]

Collection : French books 1601-1700 ; 103.2

Poésies galantes et héroïques de sieur Tristan L'Hermite : contenant ses amours, sa lyre, les plaintes d'Acante, la maison d'Astrée, la belle gueuse, l'aveugle amoureux, les terreurs nocturnes,... ([Reprod.])

Collection : French books 1601-1700 ; 103.4