Production of Bovine Hand-Made Cloned Embryos by Zygote-Oocyte Cytoplasmic Hemi-complementation

The aim of this study was to evaluate the effect of the cytoplast type and activation process on development of cloned embryos. Bovine oocytes (MII) or zygotes at the one-cell stage (IVF) were manually bisected and segregated in MII or IVF hemi-cytoplasts or hemi-karyoplasts. Adult skin cells from a bovine female were used as nucleus donors (SC). Experimental groups were composed of IVF embryos; parthenogenetic embryos; handmade cloned (HMC) embryos; and reconstructed HMC embryos using IVF hemi-cytoplast + MII hemi-cytoplast + SC (G-I); IVF hemi-cytoplast + IVF hemi-cytoplast + SC (G-II); MII hemi-cytoplast + IVF hemi-karyoplast (G-III); and IVF hemi-cytoplast + IVF hemi-karyoplast (G-IV). Embryos from G-I to G-IV were allocated to subgroups as sperm-activated (SA) or were further chemically activated (SA + CA). Embryos from all groups and subgroups were in vitro cultured in the WOW system. Blastocyst development in subgroup G-I SA (28.2%) was similar to IVF (27.0%) and HMC (31.4%) controls, perhaps due to a to a more suitable activation process and/or better complementation of cytoplasmic reprogramming factors, with the other groups and subgroups having lower levels of development. No blastocyst development was observed when using IVF hemi-karyoplasts (G-III and G-IV), possibly due to the manipulation process during a sensitive biological period. In summary, the presence of cytoplasmic factors from MII hemi-oocytes and the sperm activation process from hemi-zygotes appear to be necessary for adequate in vitro development, as only the zygote-oocyte hemi-complementation was as efficient as controls for the generation of bovine cloned blastocysts.

Star products made (somewhat) easier

We develop an approach to the deformation quantization on the real plane with an arbitrary Poisson structure which is based on Weyl symmetrically ordered operator products. By using a polydifferential representation for the deformed coordinates, xj we are able to formulate a simple and effective iterative procedure which allowed us to calculate the fourth-order star product (and may be extended to the fifth order at the expense of tedious but otherwise straightforward calculations). Modulo some cohomology issues which we do not consider here, the method gives an explicit and physics-friendly description of the star products.

Analytical Assessment of a Home Made Capillary Electrophoresis Equipment with Linear Charge Coupled Device for Visible Light Absorption Detection in the Determination of Food Dyes

The performance of modular home made capillary electrophoresis equipment with spectrophotometric detection, at a visible region by means of a miniaturized linear charge coupled device, was evaluated for the determination of four food dyes. This system presents a simple but efficient home made cell detection scheme. A computer program that converts the spectral data after each run into the electropherograms was developed to evaluate the analytical parameters. The dyes selected for analytical evaluation of the system were Brilliant Blue FCF, Fast Green FCF, Sunset Yellow FCF, and Amaranth. Separation was carried out in a 29cm length and 75 mu m I.D fused silica capillary, using 10mmolL-1 borate buffer at pH 9, with separation voltage of 7.5kV. The detection limits for the dyes were between 0.3 and 1.5mgL-1 and the method presented adequate linearity over the ranges studied, with correlation coefficients greater than 0.99. The method was applied for determination and quantification of these dyes in fruit juices and candies.

Broschyr om avändarprofiler

The project group is participating in a measuring project that is a cooperation between CIT EnergyManagement AB, the Measuring unit of Chalmers and College of Dalarna.The results from the measurementswill be put together and presented in a brochure.When designing the brochure a great deal of planning has been made how to attract the targetgroup.To make the contents of the brochure more attractive, which mainly will contain text, a lot of imageswith a symbolic substance have been used. To keep the brochure together it has been divided into fivechapters that each has been given one specific colour. The colours are consistent on initials, headlinesand images.At the moment the results of the measurements are not yet ready and the text for the brochure is notpossible to complete now. This project is expected to finish in the fall when all the measurements havebeen compiled.

Enhancing the distribution of Swedish tourism services on international markets : Possible export-ready criteria requisitioned by European tour operators

This paper identifies subjects which are relevant for Swedish suppliers of tourism services beforeapproaching foreign markets. Most suppliers are micro, small or medium sized companies anduse intermediaries, such as tour operators, for internationalization. The research considers theopinion of British and German tour operators, which require some criteria beforehand in orderto simplify both the initialization and the development of cooperation. Destination marketingorganizations (DMOs) are hereby the go-betweens since they not only represent small-scalesuppliers on international markets, but also initiate first encounters between suppliers and touroperators. Suppliers need to provide DMOs with accurate information in order to ensure thebest possible representation. After initializing collaboration, business relationships are sought todevelop in order to facilitate long-term cooperation. Proper preparation forms therefore the basefor strengthening the competitiveness of Swedish tourism prior approaching internationalmarkets. The enhancement of distributing Swedish tourism services on foreign markets appearedto be a profitable way to enable further growth, which is strongly limited on the domestic market.Increasing the export share therefore secures and further facilitates tourism’s valuablecontributions to the Swedish economy.

Assessing feasibility and acceptability of study procedures : getting ready for implementation of national stroke guidelines in out-patient health care

BACKGROUND: Even though Swedish national guidelines for stroke care (SNGSC) have been accessible for nearly a decade access to stroke rehabilitation in out-patient health care vary considerably. In order to aid future interventions studies for implementation of SNGSC, this study assessed the feasibility and acceptability of study procedures including analysis of the context in out-patient health care settings. METHODS: The feasibility and acceptability of recruitment, observations and interviews with managers, staff and patients were assessed, as well as the feasibility of surveying health care records. RESULTS: To identify patients from the the hospitals was feasible but not from out-patient care where a need to relieve clinical staff of the recruitment process was identified. Assessing adherence to guidelines and standardized evaluations of patient outcomes through health care records was found to be feasible and suitable assessment tools to evaluate patient outcome were identified. Interviews were found to be a feasible and acceptable tool to survey the context of the health care setting. CONCLUSION: In this feasibility study a variety of qualitative and quantitative data collection procedures and measures were tested. The results indicate what can be used as a set of feasible and acceptable data collection procedures and suitable measures for studying implementation of stroke guidelines in an out-patient health care context.

Shared-decision making in designing new healthcare environments : ready to take off for improved quality

Background Successful implementation of new methods and models of healthcare to achieve better patient outcomes and safe, person-centered care is dependent on the physical environment of the healthcare architecture in which the healthcare is provided. Thus, decisions concerning healthcare architecture are critical because it affects people and work processes for many years and requires a long-term financial commitment from society. In this paper, we describe and suggest several strategies (critical factors) to promote shared-decision making when planning and designing new healthcare environments. Discussion This paper discusses challenges and hindrances observed in the literature and from the authors extensive experiences in the field of planning and designing healthcare environments. An overview is presented of the challenges and new approaches for a process that involves the mutual exchange of knowledge among various stakeholders. Additionally, design approaches that balance the influence of specific and local requirements with general knowledge and evidence that should be encouraged are discussed. Summary We suggest a shared-decision making and collaborative planning and design process between representatives from healthcare, construction sector and architecture based on evidence and end-users’ perspectives. If carefully and systematically applied, this approach will support and develop a framework for creating high quality healthcare environments.

Man-Made Menopause

In this study I suggest that there are three distinct time periods mark new developments in society’s understanding of menopause, Victorian America in the mid and late nineteenth century, mid-twentieth century America, and contemporary America. This is the case not only in terms of advances in biological science, but also the ways in which the medical establishment has viewed menopause has also changed, and in terms of changes in prevalent gender assumptions. In this paper I hope to expose the ways science, history, and society has medicalized menopause, and the ways in which menopause has been viewed by individual women, their health care providers, and society as a whole more generally. I also wish to expose the fact that how we have come to think about menopause is by no means simply the result of value-free, objective science and medicine.

Explorando o potencial de uso do 'made in...' no Brasil

Este relatório, sob as óticas de Estratégia Empresarial e de Marketing, apresenta os conceitos essenciais de certificação de origem. Foram relatadas as experiências de certificação emblemática da champagne (França) e das carnes ovinas da Patagônia (Argentina). O estudo foi conduzido por meio de um estudo exploratório junto a 20 profissionais de restaurantes e estabelecimentos similares, em São Paulo, no final do ano de 1999. Os resultados permitiram concluir que, embora apresente relativa escassez de estudos associados à certificação de origem, o Brasil possui vários produtos e serviços com potencial para desenvolvimento de trabalhos dessa natureza.

O Papel dos profissionais de gestão de pessoas no processo de inclusão de pessoas com deficiência no mercado de trabalho na cidade de São Luis - MA

A inclusão de pessoas com deficiência é um desafio, não só, para as organizações, quanto também, para as pessoas com deficiência que desejam um trabalho onde possam ter seus direitos respeitados e resguardados. Nesse processo o profissional de Gestão de Pessoas tem um papel que é o de tornar o ambiente acessível a todos, mapear os cargos e postos de trabalho, sensibilizar as pessoas da empresa, capacitar os gestores para a minimização do impacto da integração ao ambientes de trabalho e domínio das ferramentas de contratação dos profissionais com deficiência, validação do programa de inclusão, e de capacitação de profissionais com deficiência. Este trabalho objetiva pesquisar se os profissionais de Gestão de Pessoas estão aptos para o desafio de implantar e desenvolver as políticas internas de inclusão das pessoas com deficiência, nas empresas em que atuam. Para tanto, foi feito uma pesquisa juntos aos profissionais responsáveis pelos programas de inclusão, em empresas com mais de 1.000 funcionários no município de São Luís – MA, para avaliar as práticas adotadas pelas organizações.

O sentido do sucesso: uma construção social made in USA

Este trabalho investiga o sucesso como um processo de construção social, a partir da análise de mais de seiscentas edições da revista Exame ao longo de três décadas. O argumento proposto na pesquisa é que o conceito de sucesso faz parte da cultura do management que, em conjunto com tecnologias administrativas, foi introduzida no País, sobretudo a partir dos anos cinquenta. A perspectiva pós-colonialista adotada permite contextualizar a importação de práticas e princípios gerenciais que compreendem o gerencialismo, a cultura do empreendedorismo e o culto da excelência. Nesse processo, destacamos o papel da mídia na difusão, legitimação e co-produção desse ideário, a partir da descrição do desenvolvimento do conceito de sucesso nos Estados Unidos e de sua reprodução no imaginário social brasileiro. Ressaltando os problemas trazidos por uma definição do sucesso ligada a aspectos extrínsecos e materiais, e reconhecendo que estudos funcionalistas ainda não ofereceram caminhos para ampliar o termo com elementos de ordem subjetiva, propomos que o sucesso seja visto como uma instituição. Por atribuirmos posição central à linguagem no processo de construção social, a base empírica desta pesquisa está fundamentada nas práticas discursivas – mais precisamente, nos repertórios linguísticos – da mídia de negócios, dado seu papel na circulação de conteúdos simbólicos. A análise dos editoriais do período de 1971 a 1998 mostrou três fases da publicação: uma em que ela se promovia; outra em que se legitimava como porta voz das empresas e uma terceira, marcada pela personalização, quando os responsáveis pelo veículo apareciam com toda sua pessoalidade, refletindo um deslocamento do foco da revista, das organizações para os indivíduos – movimento pelo qual a ideia de sucesso também passou. A análise das reportagens demonstrou que o sucesso ganhou relevância nos anos noventa e permitiu traçar um retrato do bem-sucedido segundo a Exame, a saber: como um homem empreendedor e ambicioso, branco, magro e bem aparentado, maduro nos anos setenta e jovem nos noventa, que tem alto cargo, bom salário e empregabilidade, mas vida pessoal conturbada. A análise das capas reforçou essas impressões. Se não encontramos discrepâncias na definição do sucesso ao longo da análise, percebemos a valorização do conceito e também que o sentido assumido para o termo corresponde ao sucesso norte-americano a partir dos anos trinta, relacionado à capacidade do indivíduo de impressionar, mais do que a seu caráter. No contexto brasileiro do fim do século XX, esse sucesso atende demandas de flexibilização do trabalho: cada um é um negócio e precisa se vender. A cultura do management, tão presente na publicação, justifica essa dinâmica com uma visão de mundo que sustenta um sentido do sucesso com repercussões individuais reconhecidamente negativas. Tudo isso evidencia que o Brasil absorveu um sucesso made in USA, adotado e difundido pela revista Exame. Ligado a recompensas objetivas, diante de tantas possibilidades interpretativas, esse sucesso institucionalizado atendeu interesses organizacionais, formando individualidades voltadas para esforços produtivos. Na descrição dessa dinâmica está nossa contribuição para a desnaturalização do sucesso, convidando a configurações inéditas e alternativas para o termo.

Facebook3d

INTRODUCTION With the advent of Web 2.0, social networking websites like Facebook, MySpace and LinkedIn have become hugely popular. According to (Nilsen, 2009), social networking websites have global1 figures of almost 250 millions unique users among the top five2, with the time people spend on those networks increasing 63% between 2007 and 2008. Facebook alone saw a massive growth of 566% in number of minutes in the same period of time. Furthermore their appeal is clear, they enable users to easily form persistent networks of friends with whom they can interact and share content. Users then use those networks to keep in touch with their current friends and to reconnect with old friends. However, online social network services have rapidly evolved into highly complex systems which contain a large amount of personally salient information derived from large networks of friends. Since that information varies from simple links to music, photos and videos, users not only have to deal with the huge amount of data generated by them and their friends but also with the fact that it‟s composed of many different media forms. Users are presented with increasing challenges, especially as the number of friends on Facebook rises. An example of a problem is when a user performs a simple task like finding a specific friend in a group of 100 or more friends. In that case he would most likely have to go through several pages and make several clicks till he finds the one he is looking for. Another example is a user with more than 100 friends in which his friends make a status update or another action per day, resulting in 10 updates per hour to keep up. That is plausible, especially since the change in direction of Facebook to rival with Twitter, by encouraging users to update their status as they do on Twitter. As a result, to better present the web of information connected to a user the use of better visualizations is essential. The visualizations used nowadays on social networking sites haven‟t gone through major changes during their lifetimes. They have added more functionality and gave more tools to their users, but still the core of their visualization hasn‟t changed. The information is still presented in a flat way in lists/groups of text and images which can‟t show the extra connections pieces of information. Those extra connections can give new meaning and insights to the user, allowing him to more easily see if that content is important to him and the information related to it. However showing extra connections of information but still allowing the user to easily navigate through it and get the needed information with a quick glance is difficult. The use of color coding, clusters and shapes becomes then essential to attain that objective. But taking into consideration the advances in computer hardware in the last decade and the software platforms available today, there is the opportunity to take advantage of 3D. That opportunity comes in because we are at a phase were the hardware and the software available is ready for the use of 3D in the web. With the use of the extra dimension brought by 3D, visualizations can be constructed to show the content and its related information to the user at the same screen and in a clear way. Also it would allow a great deal of interactivity. Another opportunity to create better information‟s visualization presents itself in the form of the open APIs, specifically the ones made available by the social networking sites. Those APIs allow any developers to create their own applications or sites taking advantage of the huge amount of information there is on those networks. Specifically to this case, they open the door for the creation of new social network visualizations. Nevertheless, the third dimension is by itself not enough to create a better interface for a social networking website, there are some challenges to overcome. One of those challenges is to make the user understand what the system is doing during the interaction with the user. Even though that is important in 2D visualizations, it becomes essential in 3D due to the extra dimension. To overcome that challenge it‟s necessary the use of the principles of animations defined by the artists at Walt Disney Studios (Johnston, et al., 1995). By applying those principles in the development of the interface, the actions of the system in response to the user inputs became clear and understandable. Furthermore, a user study needs to be performed so the users‟ main goals and motivations, while navigating the social network, are revealed. Their goals and motivations are important in the construction of an interface that reflects the user expectations for the interface, but also helps in the development of appropriate metaphors. Those metaphors have an important role in the interface, because if correctly chosen they help the user understand the elements of the interface instead of making him memorize it. The last challenge is the use of 3D visualization on the web, since there have been several attempts to bring 3D into it, mainly with the various versions of VRML which were destined to failure due to the hardware limitations at the time. However, in the last couple of years there has been a movement to make the necessary tools to finally allow developers to use 3D in a useful way, using X3D or OpenGL but especially flash. This thesis argues that there is a need for a better social network visualization that shows all the dimensions of the information connected to the user and that allows him to move through it. But there are several characteristics the new visualization has to possess in order for it to present a real gain in usability to Facebook‟s users. The first quality is to have the friends at the core of its design, and the second to make use of the metaphor of circles of friends to separate users in groups taking into consideration the order of friendship. To achieve that several methods have to be used, from the use of 3D to get an extra dimension for presenting relevant information, to the use of direct manipulation to make the interface comprehensible, predictable and controllable. Moreover animation has to be use to make all the action on the screen perceptible to the user. Additionally, with the opportunity given by the 3D enabled hardware, the flash platform, through the use of the flash engine Papervision3D and the Facebook platform, all is in place to make the visualization possible. But even though it‟s all in place, there are challenges to overcome like making the system actions in 3D understandable to the user and creating correct metaphors that would allow the user to understand the information and options available to him. This thesis document is divided in six chapters, with Chapter 2 reviewing the literature relevant to the work described in this thesis. In Chapter 3 the design stage that resulted in the application presented in this thesis is described. In Chapter 4, the development stage, describing the architecture and the components that compose the application. In Chapter 5 the usability test process is explained and the results obtained through it are presented and analyzed. To finish, Chapter 6 presents the conclusions that were arrived in this thesis.

Tolerância de cultivares de cana-de-açúcar a herbicidas aplicados em pós-emergência

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do programa escola ativa como política pública para escolas do campo com turmas multisseriadas: a experiência em jardim do seridó/rn (1998-2009)

This work aims at developing an evaluation of the implementation of the Program Escola Ativa as a public policy for rural schools with combined grade classrooms in Jardim do Seridó RN (1998 2009) focusing on the following dimensions: school s physical environment, training, follow up, and didactic usage of the methodology. In order to develop this research we refer to the literature that analyzes the cycle of policies (FREY, 2000). In this approach, evaluation represents an important step in the process of analyzing the implementation of public policies, as a way of measuring up their performance, as well as a guide for realignments and redefinitions (PRESSMAN; WILDAVSKY, 1998). In order to accomplish this function, the evaluator of policies must be acquainted with scientific concepts and methods that consist of describing, interpreting and analyzing the policies in the governmental sphere (MENY; THOENIG, 1992; LIMA JÚNIOR, 1978). In this perspective, we intend to investigate whether in its proposition of minimizing the blanks in the Brazilian educational system, the implementation of the Program would be contributing to the improvement of the political-pedagogical practices in the rural schools with combined grade classrooms in Jardim do Seridó RN. In order to do this research, we have developed a theoretical-methodological matrix made of analysis dimensions, variables, indicators and instruments, such as literary revision, documental analysis, semi-structured interviews with four teachers and three supervisors that work and/or have worked in Escola Ativa in that town in the period comprised among 1998 2009, besides notes taken from field observation and photographs from four rural schools with combined grade classrooms. With this research we have identified that the Program, at a national level, has gone through different phases in its implementation process, for the town was not ready to fully take the responsibilities of the autonomous expansion, in 2002. From that period on, the execution of Escola Ativa has suffered several discontinuities, such as the lack of professional training and supervising. It is also noted that the methodology contributes to the dynamization of the didactic-pedagogical activities and promotes the cooperation and autonomy of the students in the organization and the applicability of the components of the curriculum, especially of Governo Estudantil and Cantinhos de Aprendizagem. Although the directions of the Program (BRASIL, 2005) point out that Escola Ativa has among its principles social transformation, we identified that, isolated, the initiative is not capable of promoting the changes that the rural schools need, namely investments in the physical, material, pedagogical and technological infra-structure, besides the estimation and a career plan for the teachers. In a general draft of the results of this research, we realized that some aspects presented about the peculiarities of Escola Ativa in Jardim do Seridó as a governmental Program, reinforce the need for the public policies to be evaluated, in order to confront critic and operationally the planning with the practice, revising action, whenever necessary