Carbon and Nitrogen Mineralization in Soil Combining Sewage Sludge and Straw

ABSTRACT The combined incorporation of sewage sludge (SS) and oat straw (OS) to the soil can increase straw carbon mineralization and microbial nitrogen immobilization. This hypothesis was tested in two laboratory experiments, in which SS was incorporated in the soil with and without OS. One treatment in which only straw was incorporated and a control with only soil were also evaluated. The release of CO2 and mineral N in the soil after organic material incorporation was evaluated for 110 days. The cumulative C mineralization reached 30.1 % for SS and 54.7 % for OS. When these organic materials were incorporated together in the soil, straw C mineralization was not altered. About 60 % of organic N in the SS was mineralized after 110 days. This N mineralization index was twice as high as that defined by Resolution 375/2006 of the National Environmental Council. The combined incorporation of SS and OS in the soil caused an immobilization of microbial N of 5.9 kg Mg-1 of OS (mean 3.5 kg Mg-1). The results of this study indicated that SS did not increase straw C mineralization, but the SS rate should be adjusted to compensate for the microbial N immobilization caused by straw.

Effect of Nitrogen and Potassium Rates on Early Development of Macaw Palm

ABSTRACT The economic exploitation of macaw palm [Acrocomia aculeate(Jacq.) Lodd. ex Mart.] is currently in transition, from extractivism to agricultural cultivation, thus requiring studies on the fertilization of the crop. This study evaluated the response of three genotypes of macaw palm to increasing rates of nitrogen and potassium, grown in the field until the 2nd year and to establish reference contents of mineral nutrients in the leaf. The experiment was a split-plot randomized block design with five main treatments (N and K rates) and three secondary treatments (genotypes), with three replications, each plot containing three plants. Plant height, leaf number, vigor, and nutrient contents in leaf tissues were evaluated at the end of 2nd year of cultivation. Differential responses were observed among genotypes, indicating that some genotypes are more efficient in the use of mineral inputs. There was a differentiated and positive response to increasing side-dressed N and K rates in the vegetative development of macaw genotypes until the 2nd year of field cultivation, indicating variability in the species in terms of nutrient use efficiency. The N and K fertilization rate corresponding to 360 g N + 480 g K2O per plant, in four split applications over the two years of cultivation, was insufficient to induce maximum vegetative development in the three macaw genotypes. There was no variation in macro- and micronutrient contents in leaf dry matter of the three macaw genotypes.

MANAGEMENT OF IRRIGATION AND NITROGEN FERTILIZERS TO REDUCE AMMONIA VOLATILIZATION

ABSTRACT Nitrogen losses by ammonia (NH3) volatilization can be reduced by appropriate irrigation management or by alternative N sources, replacing urea. The objective of this study was to evaluate the efficiency of irrigation management and N source combinations in decreasing NH3 volatilization from an Argissolo Vermelho Distrófico típico cultivated for 28 years with black oat (Avena strigosa) and maize (Zea mays), under no-tillage in the region of Depressão Central, Rio Grande do Sul, Brazil. The experiment was arranged in a randomized block design with split plots with three replications, where the main plots consisted of irrigation systems: no irrigation; irrigation immediately before and irrigation immediately after fertilization. The subplots were treated with different N sources: urea, urea with urease inhibitor and slow-release fertilizer, at an N rate of 180 kg ha-1, broadcast over maize, plus a control treatment without N fertilization. Ammonia volatilization was assessed using semi-open static collectors for 1, 2, 4, 6, and 10 days after N fertilization. In general, more than 90 % of total NH3-N losses occurred until three days after N fertilization, with peaks up to 15.4 kg ha-1 d-1. The irrigation was efficient to reduce NH3 losses only when applied after N fertilization. However, reductions varied according to the N fertilizer, and were higher for urea (67 %) and slightly lower for urea with urease inhibitor (50 %) and slow-release fertilizer (40 %), compared with the mean of the treatments without irrigation and irrigation before fertilization. The use of urea with urease inhibitor instead of urea was only promising under volatilization-favorable conditions (no irrigation or irrigation before N fertilization). Compared to urea, slow-release fertilizer did not reduce ammonia volatilization in any of the rainfed or irrigated treatments.

Organic and Nitrogen Fertilization of Soil under ‘Syrah’ Grapevine: Effects on Soil Chemical Properties and Nitrate Concentration

ABSTRACT Viticulture is an activity of great social and economic importance in the lower-middle region of the São Francisco River valley in northeastern Brazil. In this region, the fertility of soils under vineyards is generally poor. To assess the effects of organic and nitrogen fertilization on chemical properties and nitrate concentrations in an Argissolo Vermelho-Amarelo (Typic Plinthustalf), a field experiment was carried out in Petrolina, Pernambuco, on Syrah grapevines. Treatments consisted of two rates of organic fertilizer (0 and 30 m3 ha-1) and five N rates (0, 10, 20, 40, and 80 kg ha-1), in a randomized block design arranged in split plots, with five replications. The organic fertilizer levels represented the main plots and the N levels, the subplots. The source of N was urea and the source of organic fertilizer was goat manure. Irrigation was applied through a drip system and N by fertigation. At the end of the third growing season, soil chemical properties were determined and nitrate concentration in the soil solution (extracted by porous cups) was determined. Organic fertilization increased organic matter, pH, EC, P, K, Ca, Mg, Mn, sum of bases, base saturation, and CEC, but decreased exchangeable Cu concentration in the soil by complexation of Cu in the organic matter. Organic fertilization raised the nitrate concentration in the 0.20-0.40 m soil layer, making it leachable. Nitrate concentration in the soil increased as N rates increased, up to more than 300 mg kg-1 in soil and nearly 800 mg L-1 in the soil solution, becoming prone to leaching losses.

Cover Crops and Nitrogen Fertilization Effects on Nitrogen Soil Fractions under Corn Cultivation in a No-Tillage System

ABSTRACT The use of cover crops has recently increased and represents an essential practice for the sustainability of no-tillage systems in the Cerrado region. However, there is little information on the effects of nitrogen fertilization and cover crop use on nitrogen soil fractions. This study assessed changes in the N forms in soil cropped to cover crops prior to corn growing. The experiment consisted of a randomized complete block design arranged in split-plots with three replications. Cover crops were tested in the plots, and the N topdressing fertilization was assessed in the subplots. The following cover species were planted in succession to corn for eight years: Urochloa ruziziensis, Canavalia brasiliensis M. ex Benth, Cajanus cajan (L.) Millsp, and Sorghum bicolor (L.) Moench. After corn harvesting, the soil was sampled at depths of 0.00-0.10 and 0.10-0.20 m. The cover crops showed different effects at different soil depths. The soil cultivated with U. ruziziensis showed higher contents of total-N and particulate-N than the soil cultivated with C. cajan. Particulate-N was the most sensitive to changes in the soil management among the fractions of N assessed. The soil under N topdressing showed a lower content of available-N in the 0.10-0.20 m layer, which may be caused by the season in which the sampling was conducted or the greater uptake of the available-N by corn.

Nitrogen and Potassium in Narrow-Row Cotton

ABSTRACT Information on fertilizer management for cotton in narrow-row cropping system is scarce; therefore, studies are needed to improve nutrient stewardship for such systems. The aim of this study was to evaluate the effects of nitrogen and potassium application on yield and fiber quality of cotton under a narrow-row system. A field trial was carried out for three years, where the treatments were set up in an incomplete factorial arrangement [(4 × 4) + 1] under a randomized block design, with four N rates (20, 40, 60, and 80 kg ha-1), four K2O rates (0, 40, 80, and 120 kg ha-1), and one control (no N or K2O), for a total of 17 treatments, with four replicates. Urea and potassium chloride were applied on the soil surface 20 days after crop emergence. Varieties used were FMT 701 (2009/2010 and 2010/2011) and FMT 709 (2011/2012). Cotton yield and fiber quality parameters were measured. In the narrow-row cropping system, cotton lint yield was positively affected by N and K application. Cotton yield in relation to K applications was not dependent on N rates. Potassium application increased the micronaire index and fiber resistance, whereas high N rates reduced fiber resistance.

Artificial-Intelligence-Based Optimization of the Management of Snow Removal Assets and Resources Final Report, October 2002

Geographic information systems (GIS) and artificial intelligence (AI) techniques were used to develop an intelligent snow removal asset management system (SRAMS). The system has been evaluated through a case study examining snow removal from the roads in Black Hawk County, Iowa, for which the Iowa Department of Transportation (Iowa DOT) is responsible. The SRAMS is comprised of an expert system that contains the logical rules and expertise of the Iowa DOT’s snow removal experts in Black Hawk County, and a geographic information system to access and manage road data. The system is implemented on a mid-range PC by integrating MapObjects 2.1 (a GIS package), Visual Rule Studio 2.2 (an AI shell), and Visual Basic 6.0 (a programming tool). The system could efficiently be used to generate prioritized snowplowing routes in visual format, to optimize the allocation of assets for plowing, and to track materials (e.g., salt and sand). A test of the system reveals an improvement in snowplowing time by 1.9 percent for moderate snowfall and 9.7 percent for snowstorm conditions over the current manual system.

Intestinal and hepatic nitrogen balance in the rat after the administration of an oral protein load

The fate of a small oral dose of protein given to overnight-starved rats was studied. After 3 h, 62 per cent of the protein amino acids had been absorbed. Most of the absorbed N went into the bloodstream through the portal in the form of amino acids, but urea and ammonia were also present. About one-quarter of all absorbed N was carried as lymph amino acids. The liver was able to take all portal free ammonia and a large proportion of portal amino acids, releasing urea. The hepatic N balance was negative, indicating active proteolysis and net loss of liver protein.

Estimation of soil nitrate in the spring as a basis for adjustment of nitrogen fertiliser rates

Selostus: Maassa olevan nitraattitypen arviointi simulointimallin avulla

Effects of riparian vegetation removal on nutrient retention in a Mediterranean stream

We examined the effects of riparian vegetation removal on algal dynamics and stream nutrient retention efficiency by comparing NH4-N and PO4-P uptake lengths from a logged and an unlogged reach in Riera Major, a forested Mediterranean stream in northeastern Spain. From June to September 1995, we executed 6 short-term additions of N (as NH4Cl) and P (as Na2HPO4) in a 200-m section to measure nutrient uptake lengths. The study site included 2 clearly differentiated reaches in terms of canopy cover by riparian trees: the first 100 m were completely logged (i.e., the logged reach) and the remaining 100 m were left intact (i.e., the shaded reach). Trees were removed from the banks of the logged reach in the winter previous to our sampling. In the shaded reach, riparian vegetation was dominated by alders (Alnus glutinosa). The study was conducted during summer and fall months when differences in light availability between the 2 reaches were greatest because of forest canopy conditions. Algal biomass and % of stream surface covered by algae were higher in the logged than in the shaded reach, indicating that logging had a stimulatory effect on algae in the stream. Overall, nutrient retention efficiency was higher (i.e., shorter uptake lengths) in the logged than in the shaded reach, especially for PO4-P. Despite a greater increase in PO4-P retention efficiency relative to that of NH4-N following logging, retention efficiency for NH4-N was higher than for PO4-P in both study reaches. The PO4-P mass-transfer coefficient was correlated with primary production in both study reaches, indicating that algal activity plays an important role in controlling PO4-P dynamics in this stream. In contrast, the NH4-N mass-transfer coefficient showed a positive relation-ship only with % of algal coverage in the logged reach, and was not correlated with any algal-related parameter in the shaded reach. The lack of correlation with algal production suggests that mechanisms other than algal activity (i.e., microbial heterotrophic processes or abiotic mechanisms) may also influence NH4-N retention in this stream. Overall, this study shows that logging disturbances in small shaded streams may alter in-stream ecological features that lead to changes in stream nutrient retention efficiency. Moreover, it emphasizes that alteration of the tight linkage between the stream channel and the adjacent riparian zone may directly and indirectly impact biogeochemical processes with implications for stream ecosystem functioning.

Undersowing Italian ryegrass diminishes nitrogen leaching from spring barley

Selostus: Italian raiheinä aluskasvina vähentää typen huuhtoutumista ohranviljelyssä

DNA extraction using the QIAsymphony: Evaluation of PCR inhibitor removal

The goal of this study was to compare the quantity and purity of DNA extracted from biological tracesusing the QIAsymphony robot with that of the manual QIAamp DNA mini kit currently in use in ourlaboratory. We found that the DNA yield of robot was 1.6-3.5 times lower than that of the manualprotocol. This resulted in a loss of 8% and 29% of the alleles correctly scored when analyzing 1/400 and 1/800 diluted saliva samples, respectively. Specific tests showed that the QIAsymphony was at least 2-16times more efficient at removing PCR inhibitors. The higher purity of the DNA may therefore partlycompensate for the lower DNA yield obtained. No case of cross-contamination was observed amongsamples. After purification with the robot, DNA extracts can be automatically transferred in 96-wellsplates, which is an ideal format for subsequent RT-qPCR quantification and DNA amplification. Lesshands-on time and reduced risk of operational errors represent additional advantages of the robotic platform.