Estimates of growth, mortality, recruitment pattern and MSY of important resources from the Maharashtra coast

Based on the data collected from the year 1987-1991 the growth, mortality and recruitment pattern of eighteen species of fish, two species of cephalopods and four species of penaeid prawns have been presented in the present communication. The total mortality coefficient, (Z) varied from lowest of 1.20 for O. cuvieri to a highest of 10.78 for P. stylifera. The natural mortality coefficient, (M) varied from 0.52 for T. thalassinus to 3.44 for S. crassicornis. The average annual yield of eighteen species of fish, four species of prawns and two species of cephalopods are 65.083, 38.404 and 11.373 tons as against the MSY of 83.023, 72.460 and 10.475 tons respectively. The MSY estimated for the total fish stock is 1.77.753 tons whereas the present yield is 1.14.859 tons. This indicates that higher yield can be obtained by increasing the effort.

Estimation of fishing mortality and migration coefficient of the fishery of juvenile Macrobrachium rosenbergii from Kalu River in Maharashtra

The freshwater prawn, Macrobrachium rosenbergii breeds in estuaries and the juveniles after completion of their larval stage start their upward migration towards rivers. It is at this stage fishing of juveniles takes place in river mouths. Kalu River near Titwala, in Maharashtra is estimated based on data presented by Indulkar and Shirgur (1995) for 1991 and 1992 fishing seasons. The fishing mortality was estimated to be 1.50 and 1.28 for a fishing season of 3 months in 1991 and 1992 respectively, while the migration coefficient was computed to be 3.53 during the fishing season. As the average exploitation rate during the study period was only 0.24, the juveniles are not heavily fished and there is a scope for almost doubling the present catch to about 4 million seeds per fishing season.

Mortality estimates of Indian ribbon fish Trichiurus lepturus off Maharashtra coast

In view of its new found status in export market, ribbon fish resources need to be continually monitored. Mortality, one of the important parameter is reported for the Indian ribbon fish Trichiurus lepturus Linnaeus in the present communication. The average annual instantaneous rate of total (Z), natural (M) and fishing mortality coefficient (F) were estimated as 2.66, 0.77 and 1.89 respectively for the 1995 to 1997 period. The exploitation rate (U) and exploitation ratio (E) were estimated as 0.66 and 0.71 respectively, which is beyond the optimum thrust reduction in the fishing effort for this stock along the Maharashtra coast is necessary.

Growth, mortality and yield-per-recruit of Copadichromis likomae (Cichlidae) in Lake Niassa, Mozambique

Analysis of the length-frequency data on Copadichromis likomae (Cichlidae) from Lake Niassa, Mozambique, suggests an asymptotic length of SL∞=14 cm associated with a K value of 0.93 yearˉ¹. Total and natural mortalities were estimated as 3.2 yearˉ¹ and 1.9 yearˉ¹, respectively. Yield-per-recruit analysis suggests that E=0.36 in this fishery.

Mortality rates estimates for Metapenaeus monoceros (Fabricius) of Maputo Bay

The species Metapenaeus monoceros in Maputo Bay recruits to the fishery almost all over the year, but the main recruitment occurs over a short period of time, each year, during April-May. Growth curves were constructed by following the progression of modes over a period of time. The von Bertalanffy growth parameters were estimated as L∞=31.9 mm and K(monthly)=0.25, for males, and L∞=48.3 mm and K(monthly)=0.14, for females (Brinca and Sousa, 1984). Using data on growth and length composition of the catches, the following methods were applied to estimate mortality rates of M. monoceros: estimation of natural mortality by using approximative methods; use of c.p.u.e. data; catch curves; cohort analysis; average age and length methods.

Livelihood and seed mortality related vulnerability of carp seed traders of Bangladesh

Carp seed traders in Bangladesh are considered as one of the poorest, most vulnerable and least studied sub-population. Millions of fingerlings are transported annually to various parts of the country using bus tops and/or truck-beds in open and hand agitated method by the traders and as a result they encounter mass seed mortality. They are the key players in augmenting aquaculture activities by distributing fish fry and fingerlings to the fish farmers. Assessment of the seed sources and the livelihood of the traders are important to identify their point of entry to the aquaculture sector and further improvement. Status of household asset, income profiles, and risks involved were examined using DFID's sustainable livelihood approach (SLA) framework. Tools used to collect data included group discussions (GD), semi-structured and structured household (HH) survey. Samples (n=637) were randomly drawn purposefully from 10% of the carp seed traders within 11 districts. Sampled HH were classified into six land classes.

Identification of causes of Thai pangas mortality in ponds and their control strategies

Mass mortality of Thai pangas (Pangasius hypophthalmus) is reported to be a big threat to monoculture of the species in Bangladesh. Twenty affected and twenty control Thai pangas ponds were investigated around Mymensingh district in order to identify the causes of pangas mortality. Sixty affected and sixty unaffected fish samples were examined and compared to find the fish-level variables associated with the disease. A range of haemorrhagic signs on snout, skin and fins were recorded during examination with naked eyes. Aeromonas spp. and Edwardsiella spp. were isolated from 87% and 80% of the affected fish, respectively. Even 4% of the seemingly healthy fish carried Aeromonas spp. on their skin. Among the four water quality parameters monitored, remarkably higher total ammonia (1.5 ppm) was found in water of the affected ponds compared to that of the unaffected ones (0.4 ppm). High ammonia in affected water caused by excessive organic decomposition and poor pond management might have reduced the immunity of fish, which predisposed them for bacterial invasion and consequent disease outbreak.

Multiple maternal origins of chickens: Out of the Asian jungles

Domestic chickens have long been important to human societies for food, religion, entertainment, and decorative uses, yet the origins and phylogeography of chickens through Eurasia remain uncertain. Here, we assessed their origins and phylogeographic hist

More evidence for non-maternal inheritance of mitochondrial DNA?

Background: A single case of paternal co-transmission ofmitochondrial DNA (mtDNA) in humans has been reported so far. Objective: To find potential instances of non-maternal inheritance of mtDNA. Methods: Published medical case studies (of single patients) were searched for irregular mtDNA patterns by comparing the given haplotype information for different clones or tissues with the worldwide mtDNA database as known to date-a method that has proved robust and reliable for the detection of flawed mtDNA sequence data. Results: More than 20 studies were found reporting clear cut instances with mtDNAs of different ancestries in single individuals. As examples, cases are reviewed from recent published reports which, at face value, may be taken as evidence for paternal inheritance of mtDNA or recombination. Conclusions: Multiple types (or recombinant types) of quite dissimilar mitochondrial DNA from different parts of the known mtDNA phylogeny are often reported in single individuals. From re-analyses and corrigenda of forensic mtDNA data, it is apparent that the phenomenon of mixed or mosaic mtDNA can be ascribed solely to contamination and sample mix up.

Age structure, growth and mortality estimates of an endemic Ptychobarbus dipogon (Regan, 1905) (Cyprinidae: Schizothoracinae) in the Lhasa River, Tibet

Ptychobarbus dipogon is an endemic fish in the Yarlung Tsangpo River, but its biology is poorly known. We sampled 582 specimens (total length, TL, between 70.6 and 593.0 mm) from April 2004 to August 2006 in the Lhasa River, Tibet. We estimated ages based on the counts of alternating opaque and translucent zones (annuli) in thin transverse sections of lapilli otoliths. Ages ranged from 1(+) to 23(+) years for males and 1(+) to 44(+) for females. The observed 44(+) years was the oldest reported for schizothoracine fishes. Females attained a larger size than males. The TL weight relationship was W=7.12 x 10(-6)TL(3.006) for combined sexes. The growth parameters fitted von Bertalanffy growth functions were L-infinity = 598.66 mm, k=0.0898 year(-1), t(0)=-0.7261 year and W-infinity = 1585.38 g for females and L-infinity = 494.23mm, k=0.1197 year(-1), t(0)=-0.7296 year and W-infinity = 904.88g for males. The longevities of 32.7 year for females and 24.3 year for males were similar to the observed ages. Using an empirical model we estimated the instantaneous rate of total mortality (Z) at 0.28 per year in the lower reaches. Z in the upper and middle stocks was close to the M because of unexploited or lightly exploited stock. Protracted longevity, slow growth, low natural mortality and large body size were typical characteristics of P. dipogon. The current declining trend of P. dipogon could be prevented by altering fishing regulations.

Chronic effects of water-borne PFOS exposure on growth, survival and hepatotoxicity in zebrafish: A partial life-cycle test

Perfluorooctane sulfonate (PFOS) is widely distributed and persistent in the environment and wildlife. The main aim of this study was to investigate the impact of long-term exposure to low concentrations of PFOS in zebrafish. Zebrafish fry (F-0, 14d post-fertilization, dpf) were exposed via the water for 70d to 0 (control), 10, 50 and 250 mu g L-1 PFOS, followed by a further 30d to assess recovery in clean water. The effects on survival and growth parameters and liver histopathology were assessed. Although growth suppression (weight and length) was observed in fish treated with high concentrations PFOS during the exposure period, no mortality was observed throughout the 70d experiment. Embryos and larvae (F-1) derived from maternal exposure suffered malformation and mortality. Exposure to 50 and 250 mu g L-1 PFOS could inhibit the growth of the gonads (GSI) in the female zebrafish. Histopathological alterations, primary with lipid droplets accumulation, were most prominently seen in the liver of males and the changes were not reversible, even after the fish were allowed to recover for 30d in clean water. The triiodothyronine (T-3)) levels were not significantly changed in any of the exposure groups. Hepatic vitellogenin (VTG) gene expression was significantly up-regulated in both male and female zebrafish, but the sex ratio was not altered. The overall results suggested that lower concentrations of PFOS in maternal exposure could result in offspring deformation and mortality. (c) 2008 Elsevier Ltd. All rights reserved.

Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression

Sonic hedgehog (Shh), one of important homologous members of the hedgehog (Hh) family in vertebrates, encodes a signaling molecule that is involved in short- or long-range patterning processes during embryogenesis. In zebrafish, maternal activity of Hh was found to be contributing to the formation of primary motoneurons. However, we found that all of the known Hh members were not maternally expressed in zebrafish. In the present study, full-length cDNA of common carp (Cyprinus carpio) Shh (cShh) was gained by degenerate reverse-transcription PCR (RT-PCR) and rapid amplification of cDNA ends. Sequence comparison shows that cShh coding sequence shares 93.4% identity with zebrafish Shh coding sequence, and their corresponding protein sequences have 91.9% similarity. Comparative analysis of Shh genomic sequences and Hh protein sequences from different species revealed that the genomic structures of Hh are conserved from invertebrate to vertebrate. In contrast to zebrafish Shh, cShh transcripts were detectable from one-cell stage by RT-PCR analysis. Whole mount in situ hybridization verified the maternal expression of Shh in common carp, which is, to our knowledge, the first report of that in vertebrates, suggesting that Shh might be responsible for the maternal Hh activity in common carp.

TWO SPECIFIC CAUSES OF CELL MORTALITY IN FREEZE-THAW CYCLE OF YOUNG THALLI OF PORPHYRA YEZOENSIS (BANGIALES, RHODOPHYTA)1

Porphyra yezoensis Ueda is an important marine aquaculture crop with single-layered gametophytic thalli. In this work, the influences of thallus dehydration level, cold-preservation (freezing) time, and thawing temperature on the photosynthetic recovery of young P. yezoensis thalli were investigated employing an imaging pulse-amplitude-modulation (PAM) fluorometer. The results showed that after 40 d of frozen storage when performing thallus thawing under 10 degrees C, the water content of the thalli showed obvious effects on the photosynthetic recovery of the frozen thalli. The thalli with absolute water content (AWC) of 10%-40% manifested obvious superiority compared to the thalli with other AWCs, while the thalli thawed at 20 degrees C showed very high survival rate (93.10%) and no obvious correlation between thallus AWCs and thallus viabilities. These results indicated that inappropriate thallus water content contributed to the cell damage during the freeze-thaw cycle and that proper thawing temperature is very crucial. Therefore, AWC between 10% and 40% is the suitable thallus water content range for frozen storage, and the thawing process should be as short as possible. However, it is also shown that for short-term cold storage the Porphyra thallus water content also showed no obvious effect on the photosynthetic recovery of the thalli, and the survival rate was extremely high (100%). These results indicated that freezing time is also a paramount contributor of the cell damage during the freeze-thaw cycle. Therefore, the frozen nets should be used as soon as time permits.