Alfredo Pareja Diezcanseco: la literatura es una dificultad adquirida (Testimonios)

En esta entrevista, realizada en 1987, el autor dialoga con Alfredo Pareja Diezcanseco sobre sus concepciones respecto de la literatura y la Historia. Menciona detalles de su vocación literaria, de sus lecturas iniciales y de las que, posteriormente, lo marcaron más. Pareja describe su rutina diaria como escritor, la cual se relaciona con su idea de lo que es la literatura: «una dificultad adquirida», alude al rol de los esquemas en la construcción de sus novelas, y al de la crítica de sus amigos del Grupo de Guayaquil en este proceso, durante la década del 30. Respecto de ellos, recalca que éste era esencialmente un grupo de amigos, más que un conjunto de escritores que se juntaban para trabajar en sus textos. Finalmente habla de su admiración por el historiador Arnold Toynbee, y concluye la entrevista mencionando que, para él, «la Historia siempre se hace en el subterráneo de la vida, es decir la está haciendo la vida cotidiana».

Una memoria, un testimonio: Alfredo Pareja Diezcanseco, el mecenas (Testimonios)

El texto es un testimonio de dimensiones poco abordadas de Alfredo Pareja: el lado humano del diplomático, del docente en el extranjero, de integrante del Grupo de Guayaquil. Principalmente reseña el nacimiento de un espacio clave de recuperación de la memoria: el Archivo Histórico y la biblioteca especializada del Ministerio de Relaciones Exteriores del Ecuador. Durante tres años, desde 1981, se trabajó en una pequeña oficina, elaborando fichas a partir de manuscritos, pergaminos y demás documentos que allí reposaban, Pareja veía necesario este rescate para la construcción de la historia del país. A partir de ésta y de otras fuentes -coloniales y del siglo XIX, hasta entonces desconocidas o interpretadas de diferente modo por los territorialistas ecuatorianos-, Pareja planteó una nueva interpretación del conflicto limítrofe con Perú, y sostuvo la necesidad de un acuerdo de paz, ello implicaba la aceptación del Protocolo de Río de Janeiro, situación que despertó en su contra muchos comentarios enconados, pero que formó parte de la tesis que prevaleció. El Archivo Histórico «Alfredo Pareja Diezcanseco» se inauguró en julio de 1996.

Alfredo Pareja Diezcanseco en «El jardín de la memoria» (Testimonios)

El autor brinda una faceta distinta del intelectual Alfredo Pareja, como asiduo visitante de la Librería Cima, de Quito, en los años 70. Reconstruye así la imagen del amigo generoso, del ávido lector, del erudito conocedor de autores universales, y muestra la relación del ya afamado autor de obras importantes con el dueño de la librería, en las que el énfasis radicaba en la amistad, más que en el comercio de textos. Alude también a una entrevista que le realizara. en la que Pareja insiste en la valoración que tuviera de la música como un arte superior a la literatura. Otros episodios aportan a este retrato, siempre en aquel espacio de libros, y que evidencian las cualidades humanas de Pareja Diezcanseco.

Alfredo Pareja Diezcanseco: escribir es un acto de pavor (Testimonios)

La reconstrucción de una entrevista realizada en 1988 permite al autor hacer un lúcido recorrido sobre el valor de la obra crítica, narrativa e histórico-biográfica de Alfredo Pareja Diezcanseco, y establecer un balance del aporte de la misma en varios momentos de la narrativa ecuatoriana del siglo XX: los años de las vanguardias, la década de 1950 y el período posterior al boom latinoamericano. El texto pone en relieve la talla humana de Pareja, y ofrece una muestra de esa extraña sintonía que los grandes narradores únicamente son capaces de establecer con el lector, provocando en éste la sensación de sentirse «tocado y transformado por el encanto de su palabra».

Alfredo Gangotena y la traducción: una mirada

Alfredo Gangotena produjo su obra poética en dos lenguas, el francés y el español. Por esta razón, ha existido la tendencia, en primer lugar, de desterrarlo de la historia de la literatura ecuatoriana y, en segundo, a considerar únicamente uno solo de sus dos campos de expresión. Este ir y venir entre dos lenguas y dos culturas hace que la relación entre la poesía de Alfredo Gangotena y la traducción guarden una estrecha relación, que no ha sido vista con detenimiento. Esta reflexión intenta evidenciar las relaciones entre la poesía de Gangotena, sus versiones y sus traductores, así como pretende reflexionar alrededor de la relación de Gangotena con la posibilidad de traducirse a sí mismo. Gangotena es mirado a la luz de dos teóricos de la traducción, como son Jacques Derrida y Walter Benjamin, y en la escritura de sus traductores ecuatorianos: Jorge Carrera Andrade, Gonzalo Escudero, Filoteo Samaniego, Jorge Enrique Adoum y Margarita Guarderas, a través de los cuales, con apoyo de la prensa de la época, se quiere mirar también la recepción de la obra de Gangotena en Ecuador. En definitiva, se trata de establecer algunas relaciones entre la obra de Alfredo Gangotena y la traducción.

Influence of lateral pressure to the implant bed on osseointegration: an experimental study in dogs

Aim To study osseointegration and bone-level changes at implants installed using either a standard or a reduced diameter bur for implant bed preparation. Material and methods In six Labrador dogs, the first and second premolars were extracted bilaterally. Subsequently, mesial roots of the first molars were endodontically treated and distal roots, including the corresponding part of the crown, were extracted. After 3 months of healing, flaps were elevated and recipient sites were prepared in all experimental sites. The control site was prepared using a standard procedure, while the test site was prepared using a drill with a 0.2 mm reduced diameter than the standard one used in the contra-lateral side. After 4 months of healing, the animals were euthanized and biopsies were obtained for histological processing and evaluation. Results With the exception of one implant that was lost, all implants were integrated in mineralized bone. The alveolar crest underwent resorption at control as well as at test sites (buccal aspect similar to 1 mm). The most coronal contact of bone-to-implant was located between 1.2 and 1.6 mm at the test and between 1.3 and 1.7 mm at the control sites. Bone-to-implant contact percentage was between 49% and 67%. No statistically significant differences were found for any of the outcome variables. Conclusions After 4 months of healing, lateral pressure to the implant bed as reflected by higher insertion torques (36 vs. 15 N cm in the premolar and 19 vs. 7 N cm in the molar regions) did not affect the bone-to-implant contact. To cite this article:Pantani F, Botticelli D, Garcia IR Jr., Salata LA, Borges GJ, Lang NP. Influence of lateral pressure to the implant bed on osseointegration: an experimental study in dogs.Clin. Oral Impl. Res. 21, 2010; 1264-1270.doi: 10.1111/j.1600-0501.2009.01941.x.

Single embryo and oocyte lipid fingerprinting by mass spectrometry

Methods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid ( represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation in developmental biology of the biological roles of structural and reserve lipids in embryos and oocytes should therefore benefit from these rapid MALDI-MS profiles from single and intact species.-Ferreira, C. R., S. A. Saraiva, R. R. Catharino, J. S. Garcia, F. C. Gozzo, G. B. Sanvido, L. F. A. Santos, E. G. Lo Turco, J. H. F. Pontes, A. C. Basso, R. P. Bertolla, R. Sartori, M. M. Guardieiro, F. Perecin, F. V. Meirelles, J. R. Sangalli, and M. N. Eberlin. Single embryo and oocyte lipid fingerprinting by mass spectrometry. J. Lipid Res. 2010. 51: 1218-1227.

One-step reverse transcriptase polymerase chain reaction for the diagnosis of respiratory syncytial virus in children

Human respiratory syncytial virus (HRSV) is the main cause of acute lower respiratory tract infections in infants and children. Rapid diagnosis is required to permit appropriate care and treatment and to avoid unnecessary antibiotic use. Reverse transcriptase (RT-PCR) and indirect immunofluorescence assay (IFA) methods have been considered important tools for virus detection due to their high sensitivity and specificity. In order to maximize use-simplicity and minimize the risk of sample cross-contamination inherent in two-step techniques, a RT-PCR method using only a single tube to detect HRSV in clinical samples was developed. Nasopharyngeal aspirates from 226 patients with acute respiratory illness, ranging from infants to 5 years old, were collected at the University Hospital of the University of Sao Paulo (HU-USP), and tested using IFA, one-step RT-PCR, and semi-nested RT-PCR. One hundred and two (45.1%) samples were positive by at least one of the three methods, and 75 (33.2%) were positive by all methods: 92 (40.7%) were positive by one-step RT-PCR, 84 (37.2%) by IFA, and 96 (42.5%) by the semi-nested RT-PCR technique. One-step RT-PCR was shown to be fast, sensitive, and specific for RSV diagnosis, without the added inconvenience and risk of false positive results associated with semi-nested PCR. The combined use of these two methods enhances HRSV detection. (C) 2007 Elsevier B.V. All rights reserved.

FRET peptides reveal differential proteolytic activation in intraerythrocytic stages of the malaria parasites Plasmodium berghei and Plasmodium yoelii

Malaria is still a major health problem in developing countries. It is caused by the protist parasite Plasmodium, in which proteases are activated during the cell cycle. Ca(2+) is a ubiquitous signalling ion that appears to regulate protease activity through changes in its intracellular concentration. Proteases are crucial to Plasmodium development, but the role of Ca(2+) in their activity is not fully understood. Here we investigated the role of Ca(2+) in protease modulation among rodent Plasmodium spp. Using fluorescence resonance energy transfer (FRET) peptides, we verified protease activity elicited by Ca(2+) from the endoplasmatic reticulum (ER) after stimulation with thapsigargin (a sarco/endoplasmatic reticulum Ca(2+)-ATPase (SERCA) inhibitor) and from acidic compartments by stimulation with nigericin (a K(+)/H(+) exchanger) or monensin (a Na(+)/H(+) exchanger). Intracellular (BAPTA/AM) and extracellular (EGTA) Ca(2+) chelators were used to investigate the role played by Ca(2+) in protease activation. In Plasmodium berghei both EGTA and BAPTA blocked protease activation, whilst in Plasmodium yoelii these compounds caused protease activation. The effects of protease inhibitors on thapsigargin-induced proteolysis also differed between the species. Pepstatin A and phenylmethylsulphonyl fluoride (PMSF) increased thapsigargin-induced proteolysis in P. berghei but decreased it in P. yoelii. Conversely. E64 reduced proteolysis in P. berghei but stimulated it in P. yoelii. The data point out key differences in proteolytic responses to Ca(2+) between species of Plasmodium. (C) 2011 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

Peripheral Oxidative Stress Biomarkers in Mild Cognitive Impairment and Alzheimer`s Disease

Oxidative stress has been associated with normal aging and Alzheimer`s disease (AD). However, little is known about oxidative stress in mild cognitive impairment (MCI) patients who present a high risk for developing AD. The aim of this study was to investigate plasma production of the lipid peroxidation marker, malonaldehyde (MDA) and to determine, in erythrocytes, the enzymatic antioxidant activity of catalase, glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione S-transferase (GST) in 33 individuals with MCI, 29 with mild probable AD and 26 healthy aged subjects. GR/GPx activity ratio was calculated to better assess antioxidant defenses. The relationship between oxidative stress and cognitive performance was also evaluated by the Mini Mental State Examination (MMSE). AD patients showed higher MDA levels than both MCI and healthy elderly subjects. MCI subjects also exhibited higher MDA levels compared to controls. Catalase and GPx activity were similar in MCI and healthy individuals but higher in AD. GR activity was lower in MCI and AD patients than in healthy aged subjects. Additionally, GR/GPx ratio was higher in healthy aged subjects, intermediate in MCI and lower in AD patients. No differences in GST activity were detected among the groups. MMSE was negatively associated with MDA levels (r = -0.31, p = 0.028) and positively correlated with GR/GPx ratio in AD patients (r = 0.68, p < 0.001). MDA levels were also negatively correlated to GR/GPx ratio (r = -0.31, p = 0.029) in the AD group. These results suggest that high lipid peroxidation and decreased antioxidant defenses may be present early in cognitive disorders.

Malassezia spp. in acoustic meatus of bats (Molossus molossus) of the Amazon region, Brazil

The yeasts of the Malassezia genus are opportunistic microorganisms and can cause human and animal infections. They are commonly isolated from the skin and auricular canal of mammalians, mainly dogs and cats. The present study was aimed to isolate Malassezia spp. from the acoustic meatus of bats (Molossus molossus) in the Montenegro region, `` Rondonia ``, Brazil. From a total of 30 bats studied Malassezia spp. were isolated in 24 (80%) animals, the breakdown by species being as follows (one Malassezia sp. per bat, N=24): 15 (62.5%) M. pachydermatis, 5 (20.8%) M. furfur, 3 (12.5%) M. globosa and 1 (4.2%) M. sympodialis. This study establishes a new host and anatomic place for Malassezia spp., as it presents the first report ever of the isolation of this genus of yeasts in the acoustic meatus of bats.

Advanced functionality for radio analysis in the Offline software framework of the Pierre Auger Observatory

The advent of the Auger Engineering Radio Array (AERA) necessitates the development of a powerful framework for the analysis of radio measurements of cosmic ray air showers. As AERA performs ""radio-hybrid"" measurements of air shower radio emission in coincidence with the surface particle detectors and fluorescence telescopes of the Pierre Auger Observatory, the radio analysis functionality had to be incorporated in the existing hybrid analysis solutions for fluorescence and surface detector data. This goal has been achieved in a natural way by extending the existing Auger Offline software framework with radio functionality. In this article, we lay out the design, highlights and features of the radio extension implemented in the Auger Offline framework. Its functionality has achieved a high degree of sophistication and offers advanced features such as vectorial reconstruction of the electric field, advanced signal processing algorithms, a transparent and efficient handling of FFTs, a very detailed simulation of detector effects, and the read-in of multiple data formats including data from various radio simulation codes. The source code of this radio functionality can be made available to interested parties on request. (C) 2011 Elsevier B.V. All rights reserved.