A new species of Lauromacromia (Odonata: Corduliidae) from Southeastern Brazil, with a cladistic analysis of the genus and comments on Neotropical dragonfly biogeography

Lauromacromia melanica sp. nov. from Conceicao da Barra municipality, Espirito Santo State, Brazil, is described and illustrated based on two males (both in MNRJ n degrees 135). The new species is similar to L. picinguaba differing from it mainly by the absence of pale spots on S3-6 and by the ellipsoid shape of metepisternal pale stripe. A key for males of all species of the genus is provided. A cladistic analysis encompassing 43 external morphological male characters carried out in two distinct procedures, the first with all characters unordered and the second with two or three state characters ordered. The unordered analysis generated only one most-parsimonious tree (66 steps of length, CI = 0.69, RI = 0.62). The hypothesis of monophyly of Lauromacromia is supported and includes three groups, one formed by the Atlantic Forest species (L. melanica sp. nov. + L. picinguaba), and another by the Cerrado species (L. flaviae + (L. bedei + L. luismoojeni)), and L. dubitalis, positioned in polytomy with these two groups. The ordered analysis also generated only one most-parsimonious tree (68 steps of length, CI = 0.70, RI = 0.67), which maintained the monophyly of Lauromacromia but L. dubitalis positioned basally as sister-group to the Atlantic Forest + Cerrado species groups. The geographic distribution of Lauromacromia is updated with a new record of L. luismoojeni based on one adult male (Brazil: Mato Grosso do Sul State) and probable first Brazilian records for L. dubitalis (Amazonas and Para States) based on two larvae. A vicariance hypothesis is proposed to explain spatial evolution of Lauromacromia, and based on current biogeographical classifications we consider Gomphomacromia and Rialla apart from Neotropical biota. Some aspects of biology and ecology of Lauromacromia are also discussed.

Contributions to the taxonomy of the Normanellidae (Copepoda, Harpacticoida): description of a new genus from the Brazilian continental shelf and re-assignment of Pseudocletodes vararensis Scott & Scott, 1893 (ex Nannopodidae)

A new genus and species of Normanellidae (Copepoda, Harpacticoida), Paranaiara inajae gen. et sp. nov., is described from the continental shelf off the northern coast of Sao Paulo State, Brazil. The new genus differs from the type genus Normanella Brady, 1880 and Sagamiella Lee & Huys, 1999 in its presence of lamelliform caudal rami, a maxillulary endopod represented by 2 setae, an unarmed maxillipedal syncoxa, and reduced setation on P2 enp-2 (without outer spine) and P3 enp-2 (with only 2 inner setae). All these apomorphic character states are shared with the genus Pseudocletodes Scott & Scott, 1893, formerly placed in the family Nannopodidae (ex Huntemanniidae) and here assigned to the Normanellidae. Pseudocletodes can be differentiated from Paranaiara by the loss of the P1 endopod and of the inner seta on P2-P4 enp-1, the presence of only 2 inner setae on P2 enp-2 (instead of 3) and only 1 inner seta on P4 exp-3 (instead of 2), the presence of a second inner seta on P4 enp-2 (instead of 1), the morphology of the fifth pair of legs which are not medially fused and have only 3 endopodal elements (instead of 4) in the male, and the well developed caudal ramus seta V (instead of rudimentary). It is postulated that prehensility of the P1 endopod was secondarily lost in the common ancestor of Paranaiara and Pseudocletodes. An updated family diagnosis of the Normanellidae and a dichotomous identification key to the 22 currently valid species are presented.

Description of two new species of Avicularia Lamarck 1818 and redescription of Avicularia diversipes (C. L. Koch 1842) (Araneae, Theraphosidae, Aviculariinae)-three possibly threatened Brazilian species

Avicularia diversipes (C. L. Koch 1842) known previously only from its original description is redescribed along with Avicularia sooretama sp. nov. and Avicularia gamba sp. nov. The three species are endemic to Brazilian Atlantic rainforest. With other Avicularia species, they share a procurved anterior eye row, slender embolus and medially folded spermathecae, whereas they have unusual characters, such as a very long and spiraled embolus (A. diversipes) and spermathecae with multilobular apex (A. sooretama sp. nov.). Furthermore, the three species lack a tibial apophysis in males and share a distinctive color pattern ontogeny that is not known in any other Avicularia species. The conservation status of the three species is discussed, especially with respect to endemism, illegal trafficking and habitat destruction. The creation of protected areas in southern State of Bahia, Brazil, is recommended, as well as the inclusion of these species in IUCN and CITES lists. Appendices with figures and species information are presented to facilitate correct specimen identification by custom officers, in order to limit illegal traffic.

Taxonomy and cladistic analysis of the subgenus Xenomorellia Malloch (Diptera: Muscidae: Morellia Robineau-Desvoidy) with description of two new species

Xenomorellia Malloch, a subgenus of Morellia Robineau-Desvoidy, is revised to include two new species, Morellia (Xenomorellia) inca Nihei and Carvalho sp. nov. from South America, and M. (X.) maia Carvalho and Nihei sp. nov. from Costa Rica and Mexico. Diagnoses for M. (X.) holti (Malloch) and M. (X.) montanhesa (Albuquerque) are provided, as well as an identification key to the four species of the subgenus. A cladistic analysis was performed to test the monophyly of Xenomorellia and to recover the phylogenetic relationships among its species. Tree searches resulted in one single most-parsimonious cladogram, wherein the monophyly of Xenomorellia is supported, as well as a sister-group relationship with the Neotropical subgenus Trichomorellia Stein. Xenomorellia was divided into two clades: one with Caribbean-Andean species (maia + inca), and another with species from southeastern South America (holti + montanhesa).

Diversity and quantitative analysis of Archaea in aggressive periodontitis and periodontally healthy subjects

P>Aim To investigate the diversity, levels and proportions of Archaea in the subgingival biofilm of generalized aggressive periodontitis (GAgP; n=30) and periodontally healthy (PH; n=30) subjects. Materials and methods Diversity was determined by sequencing archaeal 16S rRNA gene libraries from 20 samples (10/group). The levels and proportions of Archaea were analysed by quantitative PCR (qPCR) in four and two samples/subject in GAgP and PH groups, respectively. Results Archaea were detected in 27/28 subjects and 68% of the sites of the GAgP group, and in 26/30 subjects and 58.3% sites of the PH group. Methanobrevibacter oralis was found in all 20 samples studied, Methanobacterium curvum/congolense in three GAgP and six PH samples, and Methanosarcina mazeii in four samples from each group. The levels and proportions of Archaea were higher in GAgP than in PH, whereas no differences were observed between the two probing depth category sites from the GAgP group. Conclusion Archaea were frequently found in subjects with periodontal health and GAgP, especially M. oralis. However, the higher levels and proportions (Archaea/total prokaryotes) of this domain observed in GAgP in comparison with PH subjects indicate a possible role of some of these microorganisms as an environmental modifier in GAgP.

Phylogenetic Validation of the Genera Angomonas and Strigomonas of Trypanosomatids Harboring Bacterial Endosymbionts with the Description of New Species of Trypanosomatids and of Proteobacterial Symbionts

We comparatively examined the nutritional, molecular and optical and electron microscopical characteristics of reference species and new isolates of trypanosomatids harboring bacterial endosymbionts. Sequencing of the V7V8 region of the small subunit of the ribosomal RNA (SSU rRNA) gene distinguished six major genotypes among the 13 isolates examined. The entire sequences of the SSU rRNA and glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) genes were obtained for phylogenetic analyses. In the resulting phylogenetic trees, the symbiont-harboring species clustered as a major clade comprising two subclades that corresponded to the proposed genera Angomonas and Strigomonas. The genus Angomonas comprised 10 flagellates including former Crithidia deanei and C. desouzai plus a new species. The genus Strigomonas included former Crithidia oncopelti and Blastocrithidia cuiicis plus a new species. Sequences from the internal transcribed spacer of ribosomal DNA (ITS rDNA) and size polymorphism of kinetoplast DNA (kDNA) minicircles revealed considerable genetic heterogeneity within the genera Angomonas and Strigomonas. Phylogenetic analyses based on 16S rDNA and ITS rDNA sequences demonstrated that all of the endosymbionts belonged to the Betaproteobacteria and revealed three new species. The congruence of the phylogenetic trees of trypanosomatids and their symbionts support a co-divergent host-symbiont evolutionary history. (C) 2011 Elsevier GmbH. All rights reserved.

Molecular characterization of Mycobacterium massiliense and Mycobacterium bolletii in isolates collected from outbreaks of infections after laparoscopic surgeries and cosmetic procedures

An outbreak of infections affecting 311 patients who had undergone different invasive procedures occurred in 2004 and 2005 in the city of Belem, in the northern region of Brazil. Sixty-seven isolates were studied; 58 were from patients who had undergone laparoscopic surgeries, 1 was from a patient with a postinjection abscess, and 8 were from patients who had undergone mesotherapy. All isolates were rapidly growing nonpigmented mycobacteria and presented a pattern by PCR-restriction enzyme analysis of the hsp65 gene with BstEII of bands of 235 and 210 bp and with HaeIII of bands of 200, 70, 60, and 50 bp, which is common to Mycobacterium abscessus type 2, Mycobacterium bolletii, and Mycobacterium massiliense. hsp65 and. rpoB gene sequencing of a subset of 20 isolates was used to discriminate between these three species. hsp65 and rpoB sequences chosen at random from 11 of the 58 isolates from surgical patients and the postinjection abscess isolate presented the highest degrees of similarity with the corresponding sequences of M. massiliense. In the same way, the eight mesotherapy isolates were identified as M. bolletii. Molecular typing by pulsed-field gel electrophoresis (PFGE) grouped all 58 surgical isolates, while the mesotherapy isolates presented three different PFGE patterns and the postinjection abscess isolate showed a unique PFGE pattern. In conclusion, molecular techniques for identification and typing were essential for the discrimination of two concomitant outbreaks and one case, the postinjection abscess, not related to either outbreak all of which were originally attributed to a single strain of M. abscessus.

Microbial diversity associated with algae, ascidians and sponges from the north coast of Sao Paulo state, Brazil

Little is known about the microbial diversity associated with marine macroorganisms, despite the vital role microorganisms may play in marine ecosystems. The aim of the present study was to investigate the diversity of bacteria and fungi isolated from eight marine invertebrate and one algae samples. Data derived from ARDRA and sequencing analyses allowed the identification of marine-derived microorganisms isolated from those samples. Microbial strains identified up to the genus level revealed 144 distinct ribotypes out of 256 fungal strains and 158 distinct ribotypes out of 181 bacterial strains. Filamentous fungi were distributed among 24 different genera belonging to Ascomycota, Zygomycota and Basidiomycota, some of which had never been reported in the literature as marine invertebrate-inhabiting fungi (Pestalotiopsis, Xylaria, Botrysphaeria and Cunnninghamella). Bacterial isolates were affiliated to 41 different genera, being Bacillus, Ruegeria, Micrococcus, Pseudovibrio and Staphylococcus the most abundant ones. Results revealed an unexpected high microbial diversity associated to the macroorganisms which have been collected and suggested the selection of certain microbial taxonomic groups according to the host. The combined data gathered from this investigation contribute to broaden the knowledge of microbial diversity associated to marine macroorganisms, including as a promising source for the discovery of new natural products. (C) 2009 Elsevier GmbH. All rights reserved.

Middle permian brachiopods from central Peninsular Malaysia - faunal affinities between Malaysia and west Cambodia

A moderately diverse Permian brachiopod fauna is described from a new rock unit, the Bera Formation, in the Bera District, central Pahang, Peninsular Malaysia. The fauna consists of 19 taxa, including 14 genera and 17 (both identified and unidentified) typically Tethyan species. The fauna appears to be correlative on the basis of brachiopods with the Neoschwagerina-Yabeina fusulinid Zones in Indochina and South China. In particular, it has strong linkage to Member C (Yabeina beds) of the Sisophon Limestone, west Cambodia. This is indicated by three of the Bera species — Urushtenoidea chaoi (Ching), Spyridiophora gubleri Termier and Termier, and Transennatia termierorum sp. nov., being shared with the Cambodian fauna. A possible early Capitanian (Middle Permian) age is proposed for the Bera brachiopod fauna.

New Strophalosiidae (Brachiopoda) from the early Permian of Argentina

New representatives of the family Strophalosiidae (Brachiopoda) are described from the Tupe Formation of the Paganzo Basin, Argentina. The genus Coronalosia Waterhouse & Gupta is reviewed and the new taxa Coronalosia argentinensis sp. nov. and Tupelosia paganzoensis gen. et sp. nov. proposed. The age of the Tupe Formation is reviewed and a middle to late Asselian (Early Permian) age is preferred.
The new genus Guadalupelosia from the mid-Permian of West Texas, USA, is also proposed.

Calescharidae, a new family for the Tertiary to Recent Genera Caleschara MacGillivray and Tretosina Canu and Bassler (Bryozoa: Cheilostomata)

A new family of Cheilostomata, the Calescharidae, is introduced for the genus Caleschara MacGillivray and its Recent Australian typespecies, C. denticulata(MacGillivray), which is redefined from type and other material. The Australian Tertiary genus Tretosina Canu & Bassler and its type species,T. arcifera Canu & Bassler, are closely related,and are also assigned to the Calescharidae. The history and significance of family attributions of both genera are outlined, and RecentC. denticulata from Australian and other localities is distinguished from the Late Tertiary Victorian species C. parva Maplestone. Caleschara lithconis, sp. nov., from the Late Eocene of Victoria is one of the earliest known species: its morphology closely resembles a Recent form from the Philippines, C. junctifera Canu & Bassler. Another Recent species, Caleschara minuta (Maplestone) from the GilbertIslands, is a senior synonym of three other Indo-Pacific species,C. levinseni Harmer, C. laxa Canu & Bassler and Floridinella arculifera Canu & Bassler, and resembles the European Paleocene C. squamosa. Three other related species are briefly discussed. Close relationships to other families are problematic and are discussed; divergence in the early history of the cheilostomes is inferred.

First fossil finds of some Australian Bryozoa (Cheilostomata)

The abundant fossil record of well-preserved Bryozoa in samples from the Tertiary of Victoria and South Australia includes some 'first fossil finds' which are recorded here. Several are of species known from the Recent of the Australian or Indo-West-Pacific regions, but some represent genera with a much wider temporal and geographical range. Of the 11 species illustrated, six are known, or may be inferred, to have inhabited 'sand fauna' environments. Specimens of one species are complete enough to allow its formal description as Chlidoniopsis inopina sp. nov.

Global distribution of permian conulariids and palaeobiogeographical implications

Previously, minimal work has been carried out on conulariids due to their rare occurrences and resultant biostratigraphical limitations. The palaeobiogeographical distribution of Permian conulariids suggests that they have a marked preference for cold to cool-water regions, that they are significant indicators for migration patterns, and that they can potentially provide information on the palaeogeographical configuration and movement of terranes. Permian conulariids are found in Australia, India, New Zealand, Pakistan, Iran, Afghanistan, Kashmir, China, Japan, Russia, Germany, Canada, United States of America, and Bolivia. The diversity of Permian conulariids is markedly higher in the polar regions than in the palaeoequatorial region.

Permian conulariid genera include Notoconularia Thomas 1969, Gondaconularia Waterhouse 1986, Cheliconularia Waterhouse 1986, Neoconularia Sugiyama 1942, Calloconularia Sinclair 1952, Diconularia Sinclair 1952, Paraconularia Sinclair 1940, Mesoconularia Boucek 1939 and Conularia Sowerby 1821. This paper describes two new species of conulariids: Diconularia meadepeakensis sp. nov. from the Phosphoria Formation (Guadalupian), Idaho, USA and Paraconularia kazanensis sp. nov. from the Sokian Horizon (?Roadian), Volga Region, Russia.

A review of Australian Conescharellinidae (Bryozoa : Cheilostomata)

The family Conescharellinidae Levinsen is defined and is regarded as comprising seven cheilostome genera (Conescharellina, Bipora, Trochosodon, Flabellopora, Zeuglopora, Crucescharellina and Ptoboroa). The astogeny of colonies, that consists of frontally budded zooids with "reversed" orientation, is briefly described and compared between genera. The morphology of zooids and heterozooids is defined and keys to genera and Australian species are provided. Taxa that were first described from Australia or from reliable subsequent records are redescribed and illustrated where possible. Australian specimens that have been identified as non-Australian species, have generally been found to be distinct and are here redescribed as new species. Some non-Australian records of specimens previously assigned to Australian species have also been re-examined. These are described and sometimes referred to other taxa. Altogether, eight previously described species that have not been found in the present material are discussed and 27 taxa are described from collections, principally from the eastern and southern coasts of Australia and from the Tertiary of Victoria. Eighteen of these are considered to be new species. Where possible, type or at least topotype material of previously described species has been examined. Colonies from the collections of Museum Victoria (NMV) and the Natural History Museum, London (BMNH), have been examined. New species from Australia described here are: Conescharellina cognata, C. ecstasis, C. diffusa, C. obscura, C. stellata, C. plana, C. perculta, C. pustulosa, C. ocellata, C. macgillivrayi, C. humerus; Trochosodon fecundus, T. asymmetricus, T. diommatus, T. aster, T. anomalus, T. praecox and Crucescharellina australis. In addition, the New Zealand bryozoan Trochosodon multiarmatus (Gordon, 1989) (not Bipora multiarmata Maplestone, 1909) is described as Trochosodon gordoni sp. nov.

New lopingian (late permian) rugosochonetid species from Sichuan, South China

Two rugosochonetid species, Neochonetes (Huangichonetes) geniculatus sp. nov. and Neochonetes (Zhongyingia) linshuiensis sp. nov., are described from the Lopingian (Late Permian) of the Chuanmu section, Sichuan, South China. Ecological changes from the diverse upper Changhsingian brachiopod palaeocommunity to the depauperate post-extinction brachiopod community are briefly discussed.