Serine-1321-independent regulation of the mu 1 adult skeletal muscle Na+ channel by protein kinase C.

The adult skeletal muscle Na+ channel mu1 possesses a highly conserved segment between subunit domains III and IV containing a consensus protein kinase C (PKC) phosphorylation site that, in the neuronal isoform, acts as a master control for "convergent" regulation by PKC and cAMP-dependent protein kinase. It lacks an approximately 200-aa segment between domains I and II though to modulate channel gating. We here demonstrate that mu1 is regulated by PKC (but not cAMP-dependent protein kinase) in a manner distinct from that observed for the neuronal isoforms, suggesting that under the same conditions muscle excitation could be uncoupled from motor neuron input. Maximal phosphorylation by PKC, in the presence of phosphatase inhibitors, reduced peak Na+ currents by approximately 90% by decreasing the maximal conductance, caused a -15 mV shift in the midpoint of steady-state inactivation, and caused a slight speeding of inactivation. Surprisingly, these effects were not affected by mutation of the conserved serine (serine-1321) in the interdomain III-IV loop. the pattern of current suppression and gating modification by PKC resembles the response of muscle Na+ channels to inhibitory factors present in the serum and cerebrospinal fluid of patients with Guillain-Barré syndrome, multiple sclerosis, and idiopathic demyelinating polyradiculoneuritis.

Palmitoylation of the GluR6 kainate receptor.

The G-protein-coupled metabotropic glutamate receptor mGluR1 alpha and the ionotropic glutamate receptor GluR6 were examined for posttranslational palmitoylation. Recombinant receptors were expressed in baculovirus-infected insect cells or in human embryonic kidney cells and were metabolically labeled with [3H]palmitic acid. The metabotropic mGluR1 alpha receptor was not labeled whereas the GluR6 kainate receptor was labeled after incubation with [3H]palmitate. The [3H]palmitate labeling of GluR6 was eliminated by treatment with hydroxylamine, indicating that the labeling was due to palmitoylation at a cysteine residue via a thioester bond. Site-directed mutagenesis was used to demonstrate that palmitoylation of GluR6 occurs at two cysteine residues, C827 and C840, located in the carboxyl-terminal domain of the molecule. A comparison of the electrophysiological properties of the wild-type and unpalmitoylated mutant receptor (C827A, C840A) showed that the kainate-gated currents produced by the unpalmitoylated mutant receptor were indistinguishable from those of the wild-type GluR6. The unpalmitoylated mutant was a better substrate for protein kinase C than the wild-type GluR6 receptor. These data indicate that palmitoylation may not modulate kainate channel function directly but instead affect function indirectly by regulating the phosphorylation state of the receptor.

Electro-mechanical coupling in the aging heart: role of the Late Na+ current

The aging myopathy manifests itself with diastolic dysfunction and preserved ejection fraction. However, the difficulty in defining myocardial aging and the mechanisms involved complicates the recognition of the cellular processes underlying impaired diastolic relaxation. We raised the possibility that, in a mouse model of physiological aging, defects in the electromechanical properties of cardiomyocytes are important determinants of the diastolic properties of the myocardium, independently from changes in the structural composition of the muscle and collagen framework. Here we show that an increase in the late Na+ current (INaL) in aging cardiomyocytes prolongs the action potential (AP) and influences the temporal kinetics of Ca2+ cycling and cell shortening. These alterations increase force development and passive tension. Inhibition of INaL shortens the AP and corrects the dynamics of Ca2+ transient, cell contraction and relaxation. Similarly, repolarization and diastolic tension of the senescent myocardium are partly restored. INaL offers inotropic support, but negatively interferes with cellular and ventricular compliance, providing a new perspective of the biology of myocardial aging and the etiology of the defective cardiac performance in the elderly.

Analysis of the spectral response of fractal antennas related with its geometry and current paths

Fractal antennas have been proposed to improve the bandwidth of resonant structures and optical antennas. Their multiband characteristics are of interest in radiofrequency and microwave technologies. In this contribution we link the geometry of the current paths built-in the fractal antenna with the spectral response. We have seen that the actual currents owing through the structure are not limited to the portion of the fractal that should be geometrically linked with the signal. This fact strongly depends on the design of the fractal and how the different scales are arranged within the antenna. Some ideas involving materials that could actively respond to the incoming radiation could be of help to spectrally select the response of the multiband design.

Distortion of self-image: risk factor for obesity in children and teenagers

Introduction: Self-image is important in the behaviour and lifestyle of children and adolescents. Analysing the self-image they have and the factors that might influence their distortion, can be used to prevent problems of obesity and anorexia. The main objective of present publication was to analyse the risk factors that may contribute to self-image distortion. Material and Methods: A descriptive survey study was conducted among 659 children and adolescents in two social classes (low and medium-high), measuring height and weight, calculating BMI percentile for age and gender. Body image and self-perception were registered. Results: The percentage of overweight-obesity is higher in scholars (41.8% boys, 28.7% girls) than in adolescents (30.1% and 22.2% respectively), with no difference between socioeconomic classes. The multinomial logistic regression analysis gives a risk of believing thinner higher (p=0.000) among boys OR=2.9(95%CI:1.43-3.37), school (p=0.000) OR=2.42(95%CI:1.56-3.76) and much lower (p=0.000) between normally nourished OR=0.08(95%CI:0.05-0.13), with no differences according to socioeconomic status. The risk of believing fatter is lower (p=0.000) between boys OR=0.28(95%CI:0.14-0.57), school(p=0.072) OR=0.54(95%CI:0.27-1.6), and much higher among underweight (p=0.000) OR=9x108(95% CI:4x108-19x108). Conclusions: Are risk factors of believing thinner: males, being in a group of schoolchildren and overweight-obesity. Conversely, are risk factors of believing fatter: females, teen and above all, be thin.

The X-ray outburst of the Galactic Centre magnetar SGR J1745−2900 during the first 1.5 year

In 2013 April a new magnetar, SGR 1745−2900, was discovered as it entered an outburst, at only 2.4 arcsec angular distance from the supermassive black hole at the centre of the Milky Way, Sagittarius A*. SGR 1745−2900 has a surface dipolar magnetic field of ∼2 × 1014 G, and it is the neutron star closest to a black hole ever observed. The new source was detected both in the radio and X-ray bands, with a peak X-ray luminosity LX ∼ 5 × 1035 erg s−1. Here we report on the long-term Chandra (25 observations) and XMM–Newton (eight observations) X-ray monitoring campaign of SGR 1745−2900 from the onset of the outburst in 2013 April until 2014 September. This unprecedented data set allows us to refine the timing properties of the source, as well as to study the outburst spectral evolution as a function of time and rotational phase. Our timing analysis confirms the increase in the spin period derivative by a factor of ∼2 around 2013 June, and reveals that a further increase occurred between 2013 October 30 and 2014 February 21. We find that the period derivative changed from 6.6 × 10−12 to 3.3 × 10−11 s s−1 in 1.5 yr. On the other hand, this magnetar shows a slow flux decay compared to other magnetars and a rather inefficient surface cooling. In particular, starquake-induced crustal cooling models alone have difficulty in explaining the high luminosity of the source for the first ∼200 d of its outburst, and additional heating of the star surface from currents flowing in a twisted magnetic bundle is probably playing an important role in the outburst evolution.

The outburst decay of the low magnetic field magnetar SWIFT J1822.3−1606: phase-resolved analysis and evidence for a variable cyclotron feature

We study the timing and spectral properties of the low-magnetic field, transient magnetar SWIFT J1822.3−1606 as it approached quiescence. We coherently phase-connect the observations over a time-span of ∼500 d since the discovery of SWIFT J1822.3−1606 following the Swift-Burst Alert Telescope (BAT) trigger on 2011 July 14, and carried out a detailed pulse phase spectroscopy along the outburst decay. We follow the spectral evolution of different pulse phase intervals and find a phase and energy-variable spectral feature, which we interpret as proton cyclotron resonant scattering of soft photon from currents circulating in a strong (≳1014 G) small-scale component of the magnetic field near the neutron star surface, superimposed to the much weaker (∼3 × 1013 G) magnetic field. We discuss also the implications of the pulse-resolved spectral analysis for the emission regions on the surface of the cooling magnetar.

Still the “pivot”: Russia’s self-conception and view of the euro crisis as perceived in public opinion and the Rossiiskaya Gazeta, 2011-12. EU Centre in Singapore Working Paper No. 8, August 2012

In his influential and disputed 1904 lecture, “The Geographical Pivot of History,” Halford Mackinder argued that the Russian heartland was the fulcrum of many historical and geostrategic currents across Eurasian space. While the thesis has been thought surpassed by recent technological advances in transportation, it serves as a useful heuristic device to open certain thematic lines of analysis apparent in the presentation of the ongoing “EUrocrisis” by the country’s newspaper of record, the Rossiiskaya Gazeta.

Age determination of sediment cores of the southeastern Weddell Sea, Antarctica

On the continental margin of the southeastern Weddell Sea, Antarctica, several channel-ridge systems can be traced on the eastern side of the Crary Fan. Swath mapping of the bathymetry reveals three southwest-northeast trending ridges up to 300 m high with channels on their southeastern side. The structures occur on a terrace of the continental slope in water depths of 2000 - 3300 m. We carried out sedimentological studies on cores from three sites. Two of the studied cores are from ridges, one is from the northwestern part of the terrace. The stratigraphy of the recovered sediments is based on accelerator mass spectrometer 14C determinations, stable oxygen and carbon isotopes analyses and paleomagnetic measurements. The sediments represent a period from the last glacial maximum (LGM) to recent time. They are composed predominantly of terrigenous components. We distinguish four different sedimentary facies and assign them to processes controlling sedimentation. Microlaminated muds and cross-stratified coarse-silty sediments originated from contour currents. Bioturbated sediments reflect the increasing influence of hemipelagic sedimentation. Structureless sediments with high contents of ice-rafted debris characterize slumps. The inferred contour currents shaping the continental slope during the LGM were canalized within the channels and supplied microlaminated mud to the western sedimentary ridges due to deflection to the left induced by the Coriolis force. The lamination of the sediments is attributed to seasonal variations of current velocities. The thermohaline bottom currents were directed to the northeast and hence opposite to the Weddell Gyre. Cross-stratified coarse-silty contourites on the ridges are intercalated with the muds and indicate spillover of faster thermohaline flows. Average sedimentation rates on the terrace of the continental slope were unusually high (250 cm/ka) during the LGM, indicating active growth phases of the Crary Fan during glacial intervals. A substantial environmental change at 19.5 - 20 ka is documented in the sediments by a gradual change from lamination to bioturbation. During the recent interglacial, bioturbated sediments were deposited in all parts of the terrace. Because of a reduction of the contour current velocities (4-7 cm/s), the water masses of the Weddell Gyre, supplying fine-grained sediments from northeast, gain a greater influence on sedimentation on the continental slope. Higher percentages of microfossils indicate enhanced biogenic productivity. Increased iceberg activity is documented by greater amounts of ice-rafted debris. The interglacial sedimentation rates decrease to a few cm/ka and indicate that the Crary Fan became relatively sediment-starved during interglacial intervals.

Sea surface temperatures derived from alkenones from Late Quaternary sediments of the East Equatorial South Atlantic

Angola Basin and Walvis Ridge records of past sea surface temperatures (SST) derived from the alkenone Uk 37 index are used to reconstruct the surface circulation in the east equatorial South Atlantic for the last 200,000 years. Comparison of SST estimates from surface sediments between 5° and 20°S with modern SST data suggests that the alkenone temperatures represent annual mean values of the surface mixed layer. Alkenone-derived temperatures for the warm climatic maxima of the Holocene and the penultimate interglacial are 1 to 4°C higher than latest Holocene values. All records show glacial to interglacial differences of about 3.5°C in annual mean SST, which is about 1.5°C greater than the difference estimated by CLIMAP (1981) for the eastern Angola Basin. At the Walvis Ridge, significant SST variance is observed at all of the Earth's orbital periodicities. SST records from the Angola Basin vary predominantly at 23- and 100-kyr periodicities. For the precessional cycle, SST changes at the Walvis Ridge correspond to variations of boreal summer insolation over Africa and lead ice volume changes, suggesting that the east equatorial South Atlantic is sensitive to African monsoon intensity via trade-wind zonality. Angola Basin SST records lag those from the Walvis Ridge and the equatorial Atlantic by about 3 kyr. The comparison of Angola Basin and Walvis Ridge SST records implies that the Angola-Benguela Front (ABF) (currently at about 14-16°S) has remained fairly stationary between 12° and 20°S (the limits of our cores) during the last two glacial-interglacial cycles. The temperature contrast associated with the ABF exhibits a periodic 23-kyr variability which is coherent with changes in boreal summer insolation over Africa. These observations suggest that surface waters north of the present ABF have not directly responded to monsoon-modulated changes in the trade-wind vector, that the central field of zonally directed trades in the southern hemisphere was not shifted or extended northward by several degrees of latitude during glacials, and that a cyclonic gyre circulation has existed in the east equatorial South Atlantic over the last 200,000 years. This scenario contradicts former assumptions of glacial intensification of the Benguela Current into the eastern Angola Basin and increased coastal upwelling off Angola.

Radionuclides in sediment cores of the Atlantic sector of the Southern Ocean

We present time series of export productivity proxy data including 230Thex-normalized deposition rates (rain rates) of 10Be, dissolution-corrected biogenic Ba, and biogenic opal as well as authigenic U concentrations which are complemented by rain rates of total (detrital) Fe and sea ice indicating diatom abundances from five sediment cores across the Atlantic sector of the Southern Ocean covering the past 150,000 years. The results suggest that 10Be rain rates and authigenic U concentration cannot serve as quantitative paleoproductivity proxies because they have also been influenced by detrital particle fluxes in the case of 10Be and bulk sedimentation rates (sediment focussing) and deep water oxygenation in the case of U. The combined results of the remaining productivity proxies of this study (rain rates of biogenic opal and biogenic Ba in those sections without authigenic U) and other previously published proxy data from the Southern Ocean (231Pa/230Th and nitrogen isotopes) suggest that a combination of sea ice cover, shallow remineralization depth, and stratification of the glacial water column south of the present position of the Antarctic Polar Front and possibly Fe fertilization north of it have been the main controlling factors of export paleoproductivity in the Southern Ocean over the last 150,000 years. An overall glacial increase of export paleoproductivity is not supported by the data, implying that bioproductivity variations in the Southern Ocean are unlikely to have contributed to the major glacial atmospheric CO2 drawdown observed in ice cores.

Sea surface temperature reconstructions of sediment cores from the Atlantic sector of the Southern Ocean

Late Quaternary summer sea surface temperatures (SSTs) have been derived from radiolarian assemblages in the East Atlantic sector of the Southern Ocean. In the subantarctic and the polar frontal zone, glacial SSTs (oxygen isotope stages 2, 4, 6, and 8) were 3°-5°C cooler than today, indicating northward displacements of the isotherms about 2°-4° of latitudes. During interglacials, SSTs almost reached modern levels (oxygen isotope stages 7 and 9) or exceeded them by 2°-3°C (oxygen isotope stages 1 and 5.5). In the subantarctic Atlantic Ocean, changes in SST and calcium carbonate content of the sediment precede variations in global ice volume in the range of the main Milankovitch frequencies. Comparisons with the timing of North Atlantic Deep Water (NADW) proxy records suggests that this early response in the subantarctic Atlantic Ocean is not triggered by the flux of NADW to the Southern Ocean.

(Table 2) Species composition of agglutinated foraminifers from the abyssal zone of the Pacific Ocean

The quantitative study of distribution and taxonomic composition of recent living and dead (without plasma) benthic foraminifers revealed three foraminiferal assemblages in bottom sediments of the Pacific Ocean at depths of 3350 to 4981 m. The assemblage dominated by epibenthic Lagenammina difflugiformis, Reophax dentaliniformis, and Saccorhiza ramose occupies slopes of underwater hills. The assemblage with a high share of infaunal Cribrostomoides subglobosum, C. nitidum, and Ammobaculites agglutinans is registered on an abyssal plateau. The assemblage with a significant proportion of large Astrorhiza and Reophax species, which are characterized by active way of life, populates gentle slopes and narrow depressions with potentially strong bottom currents.

(Table 1) Conditions of vertical near-bottom profiling and the main parameters of the benthic boundary layer in several regions of the Black Sea shelf

Measurements of 14 vertical profiles of currents and hydrological parameters in the near-bottom layer with depth resolution of 0.1 m were carried out in several regions of the Black Sea shelf, at five points over the continental slope, and in three deep water regions. The upper boundary of the benthic boundary layer (BBL) was reliably determined at a point at distance from 5-7 to 35-40 m from the bottom where the gradients of density and current velocity changed. Experimental data obtained were used to determine the coefficient of bottom friction, friction velocity, coefficients of vertical diffusion of momentum and density, and vertical fluxes of temperature and salinity in the BBL.