Library outreach: addressing Utah's “Digital Divide”

A “Digital Divide” in information and technological literacy exists in Utah between small hospitals and clinics in rural areas and the larger health care institutions in the major urban area of the state. The goals of the outreach program of the Spencer S. Eccles Health Sciences Library at the University of Utah address solutions to this disparity in partnership with the National Network of Libraries of Medicine—Midcontinental Region, the Utah Department of Health, and the Utah Area Health Education Centers. In a circuit-rider approach, an outreach librarian offers classes and demonstrations throughout the state that teach information-access skills to health professionals. Provision of traditional library services to unaffiliated health professionals is integrated into the library's daily workload as a component of the outreach program. The paper describes the history, methodology, administration, funding, impact, and results of the program.

NMR evidence for the participation of a low-barrier hydrogen bond in the mechanism of delta 5-3-ketosteroid isomerase.

Delta 5-3-Ketosteroid isomerase (EC 5.3.3.1) promotes an allylic rearrangement involving intramolecular proton transfer via a dienolic intermediate. This enzyme enhances the catalytic rate by a factor of 10(10). Two residues, Tyr-14, the general acid that polarizes the steroid 3-carbonyl group and facilitates enolization, and Asp-38 the general base that abstracts and transfers the 4 beta-proton to the 6 beta-position, contribute 10(4.7) and 10(5.6) to the rate increase, respectively. A major mechanistic enigma is the huge disparity between the pKa values of the catalytic groups and their targets. Upon binding of an analog of the dienolate intermediate to isomerase, proton NMR detects a highly deshielded resonance at 18.15 ppm in proximity to aromatic protons, and with a 3-fold preference for protium over deuterium (fractionation factor, phi = 0.34), consistent with formation of a short, strong (low-barrier) hydrogen bond to Tyr-14. The strength of this hydrogen bond is estimated to be at least 7.1 kcal/mol. This bond is relatively inaccessible to bulk solvent and is pH insensitive. Low-barrier hydrogen bonding of Tyr-14 to the intermediate, in conjunction with the previously demonstrated tunneling contribution to the proton transfer by Asp-38, provide a plausible and quantitative explanation for the high catalytic power of this isomerase.

Radiation target analysis of RNA.

Ribozymes are polynucleotide molecules with intrinsic catalytic activity, capable of cleaving nucleic acid substrates. Large RNA molecules were synthesized containing a hammerhead ribozyme moiety of 52 nucleotides linked to an inactive leader sequence, for total lengths of either 262 or 1226 nucleotides. Frozen RNAs were irradiated with high energy electrons. Surviving ribozyme activity was determined using the ability of the irradiated ribozymes to cleave a labeled substrate. The amount of intact RNA remaining was determined from the same irradiated samples by scanning the RNA band following denaturing gel electrophoresis. Radiation target analyses of these data revealed a structural target size of 80 kDa and a ribozyme activity target size of 15 kDa for the smaller ribozyme, and 319 kDa and 16 kDa, respectively, for the larger ribozyme. The disparity in target size for activity versus structure indicates that, in contrast to proteins, there is no spread of radiation damage far from the primary site of ionization in RNA molecules. The smaller target size for activity indicates that only primary ionizations occurring in the specific active region are effective. This is similar to the case for oligosaccharides. We concluded that the presence of the ribose sugar in the polymer chain restricts radiation damage to a small region and prevents major energy transfer throughout the molecule. Radiation target analysis should be a useful technique for evaluating local RNA:RNA and RNA:protein interactions in vitro.

Binocular visual surface perception.

Binocular disparity, the differential angular separation between pairs of image points in the two eyes, is the well-recognized basis for binocular distance perception. Without denying disparity's role in perceiving depth, we describe two perceptual phenomena, which indicate that a wider view of binocular vision is warranted. First, we show that disparity can play a critical role in two-dimensional perception by determining whether separate image fragments should be grouped as part of a single surface or segregated as parts of separate surfaces. Second, we show that stereoscopic vision is not limited to the registration and interpretation of binocular disparity but that it relies on half-occluded points, visible to one eye and not the other, to determine the layout and transparency of surfaces. Because these half-visible points are coded by neurons carrying eye-of-origin information, we suggest that the perception of these surface properties depends on neural activity available at visual cortical area V1.

Deletion of high-avidity T cells by thymic epithelium.

Tolerance induction by thymic epithelium induces a state of so-called "split tolerance," characterized in vivo by tolerance and in vitro by reactivity to a given thymically expressed antigen. Using a model major histocompatibility complex class I antigen, H-2Kb (Kb), three mechanisms of thymic epithelium-induced tolerance were tested: induction of tolerance of tissue-specific antigens exclusively, selective inactivation of T helper cell-independent cytotoxic T lymphocytes, and deletion of high-avidity T cells. To this end, thymic anlagen from Kb-transgenic embryonic day 10 mouse embryos, taken before colonization by cells of hemopoietic origin, were grafted to nude mice. Tolerance by thymic epithelium was not tissue-specific, since Kb-bearing skin and spleen grafts were maintained indefinitely. Only strong priming in vivo could partially overcome the tolerant state and induce rejection of some skin grafts overexpressing transgenic Kb. Furthermore, the hypothesis that thymic epithelium selectively inactivates those T cells that reject skin grafts in a T helper-independent fashion could not be supported. Thus, when T-cell help was provided by a second skin graft bearing an additional major histocompatibility complex class II disparity, tolerance to the Kb skin graft was not broken. Finally, direct evidence could be obtained for the avidity model of thymic epithelium-induced negative selection, using Kb-specific T-cell receptor (TCR) transgenic mice. Thymic epithelium-grafted TCR transgenic mice showed a selective deletion of those CD8+ T cells with the highest density of the clonotypic TCR. These cells presumably represent the T cells with the highest avidity for Kb. We conclude that split tolerance induced by thymic epithelium was mediated by the deletion of those CD8+ T lymphocytes that have the highest avidity for antigen.

Examining the impact of pre-k and kindergarten classroom alignment on school readiness outcomes

This study explored children’s experiences of instructional alignment from prekindergarten to kindergarten and analyzed the impact of those alignment experiences on children’s school readiness outcomes. The study answered the following overarching research question: Does the alignment of children’s learning experiences between prekindergarten and kindergarten impact school readiness outcomes? Three sub-questions drove the research design: (1) How do children’s prekindergarten and kindergarten learning experiences align; (2) To what extent does the alignment of early learning experiences predict children’s school readiness outcomes; and (3) Does the quality of prekindergarten classroom teacher interactions moderate the impact of any PK-K alignment effects? Using cluster analysis and hierarchical linear modeling (HLM) to analyze data from over 1,300 children in the 2009 Head Start Family and Child Experiences Survey (FACES), the study found that children have distinct and definable experiences of PK-K alignment. Results also indicated a disparity in children’s PK-K alignment experiences, with Hispanic/Latino children more likely to attend Head Start programs with poor systems transition practices followed by kindergartens with poor classroom structures. The study found that growth in the use of instructional activity centers from prekindergarten to kindergarten is predictive of better literacy and math outcomes. Findings further suggested that boys, minority students, and children from lower income households are predicted to score lower than girls, white classmates, and higher-income peers across school readiness measures. Findings support the need for equitable transition and alignment practices for children from all racial and ethnic groups. They also argue for an increase in child-directed activity centers in kindergarten. With one exception, the current findings did not support the hypothesis that prekindergarten teacher quality is a moderator of alignment effects on children’s school readiness outcomes. The study presents suggestions for further research.

En torno al tratamiento lexicográfico de la fraseología humorística

Este trabajo centra su atención en la representación lexicográfica de la fraseología humorística. El humor, aun siendo un fenómeno eminentemente pragmático, forma parte del significado de algunas unidades fraseológicas (UF). Sin embargo, son confusos los criterios para considerar un fraseologismo como humorístico, lo cual provoca la disparidad de descripciones lexicográficas recogidas en distintos diccionarios. Por eso, tras un análisis de la significación fraseológica y de los puntos básicos del humor lingüístico, intentamos aunar ambos enfoques con el fin de elaborar pautas claras de determinación del carácter humorístico de algunas UF y, por ende, de la inclusión de la marca correspondiente en sus descripciones lexicográficas.

Applying reactive models to column experiments to assess the hydrogeochemistry of seawater intrusion: optimising ACUAINTRUSION and selecting cation exchange coefficients with PHREEQC

Three sets of laboratory column experimental results concerning the hydrogeochemistry of seawater intrusion have been modelled using two codes: ACUAINTRUSION (Chemical Engineering Department, University of Alicante) and PHREEQC (U.S.G.S.). These reactive models utilise the hydrodynamic parameters determined using the ACUAINTRUSION TRANSPORT software and fit the chloride breakthrough curves perfectly. The ACUAINTRUSION code was improved, and the instabilities were studied relative to the discretisation. The relative square errors were obtained using different combinations of the spatial and temporal steps: the global error for the total experimental data and the partial error for each element. Good simulations for the three experiments were obtained using the ACUAINTRUSION software with slight variations in the selectivity coefficients for both sediments determined in batch experiments with fresh water. The cation exchange parameters included in ACUAINTRUSION are those reported by the Gapon convention with modified exponents for the Ca/Mg exchange. PHREEQC simulations performed using the Gains-Thomas convention were unsatisfactory, with the exchange coefficients from the database of PHREEQC (or range), but those determined with fresh water – natural sediment allowed only an approximation to be obtained. For the treated sediment, the adjusted exchange coefficients were determined to improve the simulation and are vastly different from those from the database of PHREEQC or batch experiment values; however, these values fall in an order similar to the others determined under dynamic conditions. Different cation concentrations were simulated using two different software packages; this disparity could be attributed to the defined selectivity coefficients that affect the gypsum equilibrium. Consequently, different calculated sulphate concentrations are obtained using each type of software; a smaller mismatch was predicted using ACUAINTRUSION. In general, the presented simulations by ACUAINTRUSION and PHREEQC produced similar results, making predictions consistent with the experimental data. However, the simulated results are not identical to the experimental data; sulphate (total S) is overpredicted by both models, most likely due to such factors as the kinetics of gypsum, the possible variations in the exchange coefficients due to salinity and the neglect of other processes.

Prevalencia de depresión en pacientes con cáncer de mama. Revisión de la literatura

1.- INTRODUCCIÓN: La depresión es una sintomatología muy frecuente en los pacientes diagnosticados con cáncer de mama y su tratamiento. Se plantea una revisión de la literatura para analizar la prevalencia de la depresión en personas con cáncer de mama. 2.- METODOS: Revisión sistemática. Bases de datos: EMBASE (1999-2010) PUBMED (1981-2010). Se incluyeron los artículos que relacionaban prevalencia y depresión en cáncer de mama. 3.- RESULTADOS: Se localizaron 49 estudios; sólo 12 cumplieron los criterios de inclusión, se encontró 7 estudios descriptivos y 5 analíticos. La prevalencia de depresión encontrada en los estudios oscila entre el 6%-57%. 4.- CONCLUSIONES: La disparidad de datos obtenidos conlleva a la falta de una evidencia empírica de la prevalencia de la depresión en personas con cáncer de mama.

La gastronomía como Industria Creativa en un contexto digital. Análisis de webs y redes sociales de los restaurantes españoles con estrella Michelin

En los últimos años es evidente el éxito creciente de la gastronomía, no sólo como mercado económico sino también desde una perspectiva comunicativa. Entendida como una Industria Creativa, la gastronomía está adquiriendo cada vez más peso en el mercado de la comunicación y se están desarrollando planes estratégicos que acercan progresivamente el sector gastronómico a todos los públicos, democratizando el arte de la cocina y haciendo de éste un talento accesible y disponible para todos los públicos. En pleno contexto de crecimiento, las estrategias y recursos comunicativos que utilizan tanto los mass media como los propios restaurantes y profesionales de la cocina, se han de convertir en objeto de estudio necesario para entender, por ejemplo, de qué manera se explotan los recursos comunicacionales y cuál es el alcance de los mismos así como sus oportunidades. El presente artículo se centra en un análisis de contenido de las páginas webs y las redes sociales utilizadas por los ocho restaurantes españoles galardonados con tres estrellas por la Guía Michelin España-Portugal (2014), sometidos a estudio en la misma semana (del 15 al 21 de septiembre). El objetivo principal es tratar de conocer estrategia de comunicación online llevada a cabo por estos restaurantes y establecer la importancia que se les atribuye a los recursos web a la hora de contribuir tanto a la consolidación de sus propias marcas como a la propia Industria Creativa gastronómica. Los resultados muestran cierta disparidad en el uso tanto de los recursos web como de los social media y avanzan que el sector gastronómico y de la restauración española tiene un reto: seguir comunicando implicándose en mayor medida con la bidireccionalidad e invitación a la participación de sus públicos, con el objetivo de captar nuevos contactos, fidelizar a los clientes actuales y convertir a todos ellos en prescriptores de sus servicios.

La desigualdad entre mujeres y hombres en el sector hostelero español

Es un lugar común afirmar que una de las repercusiones positivas del desarrollo turístico es la facilitación de la incorporación de la mujer al mundo laboral. Así, las mujeres representan la mayor parte de la fuerza de trabajo del sector hostelero español y están representadas en todas las categorías laborales. Sin embargo, se sabe que dicha incorporación se realiza en condiciones desiguales y, específicamente en los hoteles, las mujeres ocupan mayoritariamente puestos de baja cualificación. También es un lugar común que una de las claves para el éxito del sector turístico es asegurar que los trabajadores sean eficientes y estén comprometidos con la empresa para garantizar la calidad del servicio prestado. Las políticas laborales deben promover una mayor igualdad de oportunidades, remuneración y condiciones de trabajo en el sector. En este trabajo se presentan evidencias de la disparidad salarial, la segregación vertical y la mayor precariedad laboral que sufren las mujeres en el subsector de la hostelería en España. Con este propósito se analizan datos estadísticos secundarios procedentes de organismos oficiales. Finalmente, se propone una reflexión acerca de las razones que motivan estas desigualdades, así como de las políticas que deberían seguirse para mitigarlas.

Tiempos de equidad de género: descripción de las desigualdades entre comunidades autónomas, España 2006-2014

Objetivo: La equidad de género es un determinante estructural de las desigualdades en salud. Por ello, se pretende visibilizar su evolución en las comunidades autónomas (CC.AA.) desde 2006, previamente a la promulgación de la Ley de Igualdad (2007) y la crisis económica (2008), hasta 2014. Método: Estudio ecológico sobre la equidad de género en las 17 CC.AA. en 2006-2011-2014. Cálculo de: 1) índice de equidad de género modificado (IEGM) de las CC.AA. (0 = equidad, ±1 = inequidad); 2) convergencia interregional y temporal en equidad de género. Resultados: El IEGM de las CC.AA.2014 toma valores negativos próximos a 0 (inequidad desfavorable a las mujeres). No hay convergencia interregional en la equidad de género, pues aumenta la dispersión (2006: 0,1503; 2011: 0,2280; 2014: 0,4964). Tampoco existe convergencia temporal, al no evolucionar mejor las CC.AA. menos equitativas. La brecha de género en actividad económica sigue desfavorable a las mujeres. En 2006-2011 disminuye en todas las CC.AA., y en 2014 aumenta en seis CCAA. La brecha de género en educación tiene valores positivos próximos a 0 (desfavorable a los hombres) en 2006-2011-2014, y en empoderamiento es desfavorable a las mujeres, siendo la dimensión que más pesa en la equidad de género. Se mantiene la dispersión entre CC.AA. en 2006-2014 en actividad económica y educación, y aumenta en empoderamiento. Conclusiones: El contexto de equidad de género alcanzado en las CC.AA. españolas en 2006 se ha perdido durante la crisis económica, al aumentar la desigualdad en la equidad de género entre CC.AA. en 2014. La inequidad de género sigue siendo desfavorable a las mujeres.

(Table 2) Planktic foraminifera and their diversity indices of sediment surface samples from the Atlantic Ocean

Species distribution patterns in planktonic foraminiferal assemblages are fundamental to the understanding of the determinants of their ecology. Until now, data used to identify such distribution patterns was mainly acquired using the standard >150 µm sieve size. However, given that assemblage shell size-range in planktonic foraminifera is not constant, this data acquisition practice could introduce artefacts in the distributional data. Here, we investigated the link between assemblage shell size-range and diversity in Recent planktonic foraminifera by analysing multiple sieve-size fractions in 12 samples spanning all bioprovinces of the Atlantic Ocean. Using five diversity indices covering various aspects of community structure, we found that counts from the >63 µm fraction in polar oceans and the >125 µm elsewhere sufficiently approximate maximum diversity in all Recent assemblages. Diversity values based on counts from the >150 µm fraction significantly underestimate maximum diversity in the polar and surprisingly also in the tropical provinces. Although the new methodology changes the shape of the diversity/sea-surface temperature (SST) relationship, its strength appears unaffected. Our analysis reveals that increasing diversity in planktonic foraminiferal assemblages is coupled with a progressive addition of larger species that have distinct, offset shell-size distributions. Thus, the previously documented increase in overall assemblage shell size-range towards lower latitudes is linked to an expanding shell-size disparity between species from the same locality. This observation supports the idea that diversity and shell size-range disparity in foraminiferal assemblages are the result of niche separation. Increasing SST leads to enhanced surface water stratification and results in vertical niche separation, which permits ecological specialisation. Specific deviations from the overall diversity and shell-size disparity latitudinal pattern are seen in regions of surface-water instability, indicating that coupled shell-size and diversity measurements could be used to reconstruct water column structures of past oceans.

Morphometric measurements throughout ontogeny of selected planktic foraminiferal test species

Development plays an important part in shaping adult morphology and morphological disparity, yet its influence on evolutionary processes is seldom explored because of a lack of preservation of ontogenetic stages in the fossil record. By preserving their entire ontogenetic history within their test, and with the advent of high-resolution imaging techniques, planktic foraminifera allow us to investigate the influence of developmental constraints on disparity. Using Synchrotron radiation X-ray tomographic microscopy (SRXTM), we reconstruct the ontogenetic progression of seven species across several of the major morphotypic groups of planktic foraminifera, including morphotypes of a species exhibiting high phenotypic plasticity and closely related pseudo-cryptic sister-taxa. We show differences in growth patterns between the globigerinid species, which appear more tightly regulated within the framework of isometry from the neanic stage, and the globorotaliid species, whose adult stages present allometric trends. Morphological change through ontogeny results in a change in surface area to volume ratios. Different metabolic processes therefore dominate at different stages of ontogeny, changing the vulnerability of the organism to environmental influences over growth, from factors affecting diffusion rates in the juvenile to those affecting energy supply in the adult. These findings identify some of the parameters within which evolutionary mechanisms have to act.

Heat flow determinations of ODP Hole 111-504B

Hole 504B in the eastern equatorial Pacific has been the focus of five scientific drilling expeditions since it was first drilled in 1979. During these five legs, a series of temperature logs has been obtained over a time span of almost 8 yr, documenting the geothermal and hydrologic state of the oceanic crust in this region. Immediately following reentry at the onset of ODP Leg 111 operations, a high-resolution temperature probe was lowered into the borehole and a precise record of temperature vs. depth in Hole 504B was recorded down to 1300 mbsf. As was observed during previous legs, the temperature gradient in the upper 400 m was reduced, indicating that downhole flow of cool ocean waters through the casing continued, though at a diminished rate. As subhydrostatic pressures in the upper basement have gradually diminished, the volume of flow has decayed from an estimated 6000-7000 L/hr in late 1979 to about 80 L/hr during Leg 111. At depths below 480 mbsf, a predominantly conductive heat transfer environment enabled the temperature gradient log to be analyzed with respect to lithology on both fine and broad scales. Anomalies in the gradient log in the cased section through the sedimentary column were found to correspond to biostratigraphic age markers and/or sharp changes in sediment composition and texture. Broad variations in temperature gradient within the basement correlated with large-scale porosity trends. Conductive heatflow estimates depict a systematic reduction with depth, ranging from approximately 196 mW/m**2 in the sediments to 120 ± 17 mW/m**2 at 1300 mbsf. Possible causes for this observation were examined from several perspectives, but none was suitably convincing. A fluid instability analysis indicated the likely existence of convection cells within the borehole and substantiated the hypothesis of mixing within the borehole postulated from isotopic and chemical studies of borehole waters. However, such mixing of borehole fluids does not provide an adequate explanation for the heatflow variations, and the disparity between surficial and deep values of heat flow remains unresolved.