Tumor hepático experimental (VX-2) em coelho: implantação do modelo no Brasil

Os estudos para a investigação de novas modalidades terapêuticas em biologia tumoral, deveriam passar por estudos experimentais prévios. Neste sentido dispõem-se hoje de uma grande variedade de modelos tumorais experimentais; em determinadas investigações faz-se necessária a adequação do modelo tumoral às necessidades biológicas, patológicas e experimentais dos estudos. Desta forma, em nosso serviço, buscávamos um modelo tumoral hepático para estudos experimentais que se adequasse às seguintes características: fácil manipulação, crescimento controlável, evolução e agressividade semelhantes aos seres humanos. Os dados da literatura nos levaram a busca do tumor hepático VX-2, em coelhos. Neste artigo discutimos as vantagens da utilização deste modelo experimental e a sua introdução em nosso país.

Perfil da clientela de um programa de pronto-atendimento psicológico a estudantes universitários

Pesquisas indicam que a caracterização da clientela de clínicas-escola de Psicologia fornece elementos para o aprimoramento dos serviços oferecidos. O presente estudo propõe-se a traçar o perfil sociodemográfico e clínico dos usuários do Programa de Pronto-Atendimento Psicológico ao Aluno da UNESP/Assis. Os dados indicam que a maioria dos usuários era ingressante na universidade, oriunda do curso de Psicologia, com idade entre 19 e 22 anos, do gênero feminino, não exercia atividade profissional remunerada, apresentava dificuldades psicológicas moderadas e foi encaminhada para psicoterapia. Tal caracterização subsidiou a implementação de mudanças nos serviços já existentes e a criação de novas modalidades de atendimento.

Desempenho e rendimento de carcaça de frangos de corte submetidos a diferentes níveis de treonina e lisina, na fase final de criação

Este experimento foi realizado com o objetivo de verificar o desempenho e características de carcaça de frangos de corte, submetidos à diferentes níveis de lisina e treonina, na fase final de criação (42 a 56 dias de idade). Novecentos e setenta e dois frangos machos foram distribuídos em um delineamento inteiramente casualizado, divididos em nove tratamentos em um esquema fatorial 3x3 (três níveis de treonina; 0,70; 0,77 e 0,84% e três níveis de lisina; 0,94; 1,04 e 1,14%), com quatro repetições por tratamento, de 27 aves cada. Ganho de peso, consumo de ração, conversão alimentar, rendimento de carcaça e gordura abdominal foram avaliados. O desempenho e rendimento de carcaça, apesar de não terem sido afetados pelos tratamentos, mostraram-se com tendência de melhora com o aumento dos níveis de adição dos aminoácidos estudados.

Crescimento pós-natal do coelho Norfolk: correlação entre parâmetros somáticos e área dos tipos de fibras musculares

Skeletal muscle fibers areas, the weight of body, carcass and muscle, the gastric content and the length of intestines and caecum of Norfolk rabbit from neonate to 26th week were measured. Types of fibers and areas were studied in -70-degrees-C frozen blocks of flexor carpo radialis muscle. Some sections were stained with Haematoxylin and Eosin and others were submitted to SDH, NADH-TR and m-ATPase, followed by acid and alkali preincubations. All parameters revealed a high degree of correlation. Carcass weight/body weight ratio reached 55,1% at 10th week. From neonate to 26th week, the areas of SO, FOG and FG muscle fibers increased of x9.1, x12.4 and x22.2. While SO and FOG fibers tend to stabilize their growth at 10th week, FG fibers were active at this age. Due to early stabilization of SO and FOG fibers growth, the additional increase in muscle tissue in attributed mainly to hypertrophy of FG fibers.

Caracterização morfológica dos estádios de desenvolvimento do aparelho reprodutor masculino da rã-touro, Rana catesbeiana, no sistema anfigranja de criação intensiva

The objective of this work was to caracterize the morphological characterization of the development stages of male reproduction apparel of bullfrog Rana catesbeiana from intensive Anfigranja system. During the period of one year, 657 bullfrog males were removed from regrowing and reproduction sections in Anfigranja system to study basic aspects of the reproduction of this specie. The collections were realized monthly and, in that occasion, the body and testicles weights for each animal were recorded and their macroscopic gonadal characteristics, registred. Testicles from 274 males were removed and histologically analyzed. From the macro and microscopic gonadal characteristics, analysis set of the testicles, five stages of gonadal maturation for the male of Rana catesbeiana were determined: juvenile, beginning of maturation, intermediate maturation, advanced maturation and spent.

Estimativa do Tamanho da Primeira Maturação Sexual da Rã - Touro, Rana catesbeiana, no Sistema Anfigranja de Criação Intensiva

The objective of this research was to study the basic aspects of bullfrog reproduction in Anfigranja system from June 1989 to July 1990. For the 1493 animals involved in this experiment (657 males and 836 females) biometrical data were obtained (weight, length and tympanum diameter) and developmental stages of gonads were determined. Female sizes at first maturation were 10,33 cm and 109,78 g and for males it was around 8,09 cm; all males above 45 g were already in the maturation process. The relationship between tympanum diameter and body length (RTC) was considered a good index for male maturation.

Caracterização morfológica dos estádios de desenvolvimento do aparelho reprodutor feminino da rã-touro, Rana catesbeiana, no sistema anfigranja de criação intensiva

The objective of this work was to caracterize the morphological characterization of the development stages of female reproduction apparel of bullfrog Rana catesbeiana from intensive Anfigranja system. During the period of one year, 836 bullfrog females were removed from regrowing and reproduction sections of the Anfigranja system to study the basic aspects of the reproduction of this specie. The collections were realized monthly and, in that occasion, the body and ovary weights for each animal were recorded and their macroscopic gonadal characteristics, registred. The ovaries from 303 females were removed and histologically examined. From the macro and microscopic characteristics analysis set of the ovary, five stages of gonadal maturation for the female of Rana catesbeiana were determined: juvenile, beginning of maturation, intermediate maturation, advance maturation and spent.

Efeito do período de jejum na fase final de criação de frangos de corte machos sobre desempenho e mortalidade

The objective of this work was to evaluate effect of fasting period in the last growing phase on performance and mortality of male broilers. Two thousand one-day old male chicks were distributed in five randomized blocks according to a 4x2 factorial. (Four feeding programs (FP): ad libitum or one of three fasting schedules: 8-12, 12-16 and 8-16; and two strains (S): Ross or Hubbard-Peterson). Fifty birds were used per replicate. Birds were raised under identical feed and management conditions until day 42. During the experimental period (days 43-56), average temperature, relative humidity and temperature-humidity index (THI) recorded at day and at night were 29.4°C, 57.7 % and 78.1; and 22.0°C, 73.1% and 67.4, respectively. No SxP interaction was observed for all characteristics studied. At day 56, the Ross broilers showed higher mean weight (3104 g), weight gain, WG (3059 g), feed/ gain ratio, FC (2.22) and production factor, PF (213), than the Hubbard-Peterson birds (3011, 2967, 2.28 and 194, respectively). During the experimental period, the Ross broilers showed higher WG (831 g) and FC (3.40) than the Hubbard-Peterson ones (770 and 3.67, respectively). Birds submitted to the 8-12 h fast showed higher feed intake (2864 g) than those that fasted during the 8-16 period (2690). No effect of P on mortality was observed, either considering the overall period (days 1-56) or the experimental period (days 43-56). Fasting in the last phase of rearing not aided by other practices failed to decrease heat stress effects on performance and mortality of male broilers.

Características estruturais da parede das artérias renal e femoral de coelho (Oryctolagus cuniculus)

In the renal and femoral arteries of rabbit was verified that both vessels had walls structured by myostromal components, despite of their different distributive of blood, being the renal artery a visceral blood vessel and the femoral artery a parietal vessel. This wall pattern in these vessels concerned to presence of connective stromal elements (collagen and elastic fibres and lamellae) and smooth muscle cells coexisting with some equilibrium in the wall structure of the renal and femoral arteries, mainly in the medial layer architecture. An intimal folding pattern was verified around the vascular lumen, possibly related to capacitance of the both arteries regarding to variability of pressure levels in cardiac cycle. Furthermore, myostromal relations of connective elements and smooth muscle cells verified in the medial layer and the network formed by connective elements in the adventitial layer of these arteries contributed to maintenance of wall viscoelasticity properties of the vessels.

Uso de polinômios segmentados para o estudo da curva de lactação de búfalos da raça Murrah e seus mestiços em sistema de criação semi-intensivo, no estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Qualidade química da água residual da criação de peixes para cultivo de alface em hidroponia

Aiming to evaluate the association of hydroponic lettuce cultivation with residues from a fish intensive breeding system, a project was carried out in the Aquaculture Center in the FCAV-UNESP at Jaboticabal, SP, Brazil. A closed system was designed in order to allow the water to circulate through the fish tanks, a clarifier tank for removal of residues, a reservoir for biological conversion of ammonia into nitrate, and the hydroponic system. After this process, water returned back to the fish tanks. Three varieties of lettuce, constituting the treatments with four repetitions were evaluated. The results of the chemical analysis of the residual water from the fish tanks indicated the presence of the majority of the mineral nutrients necessary for vegetable development. Their concentration was close to that found in nutrient solutions, used for lettuce hydroponic cultivation, except for potassium and magnesium. The low concentration of magnesium in the water did not prevent lettuce development, although the plants presented visual symptoms of deficiency of this nutrient. Differences were not found between the varieties produced, regarding productivity and the mean weight of fresh plants.

Analisando imagens: Um ensaio sobre a criação de fontes narrativas para compreender os Grupos Escolares

This paper is an essay on how photos can be analysed and used to create narratives which may serve as resources for historical studies about school practices. As an exercise, we deal with six old photographs taken in Grupos Escolares, a Brazilian educational institution founded in the last decade of the 19th Century and extinguished in the 1970's. According to some authors, these schools represented the beginning of the public educational system in Brazil.

Desenvolvimento de metodologia de criação e multiplicação de Aphis gossypii: Avanços e sucessos

Estudos sobre as mais diversas formas de controle de pragas e utilização de inimigos naturais, exigem artrópodes em quantidade e qualidade. Mediante essa necessidade, o processo de criação e multiplicação de artrópodes, vem ao longo dos anos, passando por significativas transformações. As técnicas de criação e multiplicação de insetos são muito importantes em estudos de biologia e de métodos de controle. A criação e definição de uma metodologia para multiplicação tem sido um grande desafio nos estudos da biologia e da criação de insetos, principalmente, devido a dificuldade de estabelecimento de populações em casa de vegetação e campo. Visando otimizar e adequar uma metodologia de multiplicação de Aphis gossypii (Hemiptera: Aphididae) em condições de laboratório, foi estabelecida uma colônia sobre plantas de algodoeiro (Gossypium hirsutum L. raça Latifolium Hutch) objetivando uma melhoria nas condições de sobrevivência e multiplicação desses insetos, menores influencias dos fatores abióticos, e a potencialização do uso das plantas por um período mais longo. Com o desenvolvimento dessa técnica e dos recursos utilizados na criação, notou-se um incremento na quantidade de indivíduos obtidos, melhor aproveitamento das plantas e diminuição de custos. Dessa forma evitou-se também a migração e infestação de outras espécies de pulgões e a ação de inimigos naturais. Após o estabelecimento da criação, verificou-se que o controle do parasitismo de A. gossypii foi de acordo com as condiçõ es submetida.

Parâmetros físico-químicos da água e ocorrência de streptococcus spp. em criação intensiva de tilápia no estado do espírito Santo, Brasil

This study evaluated the influence of intensive farming of tilapia on physical and chemical parameters and on the occurrence of Streptococcus spp. in the water of the lake and of cages. Throughout a year, monthly samplings were taken in the rainy and dry seasons for a year, at two sampling sites, lake and net cages. For the determination of water quality, physical and chemical water parameters were evaluated and compared to the standards established by Conama Resolution no. 357/2005. The presence of Streptococcus spp. in the water was determined by plating on blood Agar and biochemical screening. Mean values of water parameters were tested using the Kruskal-Wallis test comparing sampling sites and seasons. Ammoniacal nitrogen (ammoniacal-N), total phosphorus (total-P) levels and occurrence of Streptococcus spp. have increased in the water of the net cages. The mean values of several parameters have decreased during the rainy period, except for pH, temperature and ammoniacal-N. Total-P and dissolved oxygen levels, during dry and rainy periods, respectively, exceeded the standard established for freshwater class 2, recommended for aquaculture, which can be harmful to the fish. Therefore, constant monitoring of the physical,chemical and microbiological water parameters is recommended since the Juara lake is also used for recreational purposes.