Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl2 in Qinghai-Tibetan plateau mammals and sea level mice

Ochotona curzoniae and Microtus oeconomus are the native mammals living on the Qinghai-TibetanPlateau of China. The molecular mechanisms of their acclimatization to the Plateau-hypoxia remain unclear. Expressions of hepatic hypoxia-inducible factor (HIF)-1 alpha, insulin-like growth factor-I (IGF-I)/IGF binding protein (BP)-1(IGFBP-1; including genes), and key metabolic enzymatic genes [lactate dehydrogenase (LDH)-A/isocitrate dehydrogenase (ICD)] are compared in Qinghai-Tibetan- Plateau mammals andsea- level mice after injection of CoCl2 (20, 40, or 60 mg/ kg) and normobaric hypoxia (16.0% O-2, 10.8% O-2, and 8.0% O-2) for 6 h, tested by histochemistry, Western blot analysis, ELISA, and RT-PCR. Major results are CoCl2 markedly increased 1) HIF-1 alpha only in mice, 2) hepatic and circulatory IGF-I in M. oeconomus, 3) hepatic IGFBP-1 in mice and O. curzoniae, and 4) LDH-A but reduced ICD mRNA in mice (CoCl2 20 mg/kg) but were unchanged in the Tibetan mammals. Normobaric hypoxia markedly 1) increased HIF-1 alpha and LDH-A mRNA in mice and M. oeconomus (8.0% O-2) not in O. curzoniae, and 2) reduced ICD mRNA in mice and M. oeconomus (8.0% O-2) not in O. curzoniae. Results suggest that 1) HIF-1 alpha responsiveness to hypoxia is distinct in lowland mice and plateau mammals, reflecting a diverse tolerance of the three species to hypoxia; 2) CoCl2 induces diversities in HIF-1, IGF-I/IGFBP-1 protein or genes in mice, M. oeconomus, and O. curzoniae. In contrast, HIF-1 mediates IGFBP-1 transcription only in mice and in M. oeconomus (subjected to severe hypoxia); 3) differences in IGF-I/IGFBP-1 expressions induced by CoCl2 reflect significant diversities in hormone regulation and cell protection from damage; and 4) activation of anaerobic glycolysis and reduction of Krebs cycle represents strategies of lowland-animals vs. the stable metabolic homeostasis of plateau- acclimatized mammals.

黄土高原中部60万年来黄土—古土壤序列有机碳同位素研究

Vegetation is very sensitive to climate change. Carbon isotopes in paleosol have been widely used to contruct the propotion of plants using C3 and C4 photosynthetic pathways. δ13C of Loess organic matter were analyzed on the loess- paleosols samples from Jingchuan sections and Luochuan S4—S5 sequence. This paper presents a long carbon isotope time series, covering the last 600kyr. δ13C record of Loess organic matter in Jingchuan is correlated with marine oxygen isotope records. Basing on former research work, this paper discusses temperature, rainfall and P CO2 effect on δ13Corg value. In the interglacial periods, carbon isotope is more sensitive than other proxies and indicates several climate fluctuations. The main conclusions are as follows: 1. Obtained δ13C composition from paleosols and loess sediments in Jingchuan range of -20.0‰ to -24.6‰, the maximum biomass of C4 is 35%, indicating a C3 and C4 mixed steppe with C3 dominated. C4 plant is not always expansion during paleosols periods. The minimum values of Jingchuan section appeared in S4 soil, and the vegetation was almost pure C3 plant at that time. δ13Corg value in S5-2 is also lower than loess in S5, reaching the minimum valus of S5 soil. 2. PCO2 variation has little impact on δ13Corg value in interglacial periods for the last 600kyr. The correlation between δ13Corg value curve and magnetic susceptibility curve as proxy of summer monsoon in general, means summer monsoon drive C4 plant expansion during glacial and interglacial. 3. The lowerδ13Corg values in S4 and S5-2 appear at Jingchuan and Luochuan, suggest origin from woodland or C3 grassland. Whatever vegetation it is, indicate strengthened East Asian summer monsoon and increase of precipitation. C4 plant percentage is lower in S5-1 and S1 which have stronger summer monsoon, than S0 and S2. And it also indicates increase of precipitation.δ13Corg values has not always non-linearity correlation with summer monsoon. 4. The maximum entroy spectral analysis of δ13C values of the last 600kyr indicates there is 21 kyr cycles in Loess sequence. It means that summer monsoon in the Chinese Loess Plateau also has the precession cycles like its origin low latitude.

Thioreduction of cyclopentanone and hydrodesulfurization of dibenzothiophene over sulfided nickel or cobalt-promoted molybdenum on alumina catalysts

Two series of sulfided Ni or Co promoted Mo/alumina catalysts, having different Ni or Co loadings, were characterized by their activities for the transformation of cyclopentanone into cyclopentanethiol (flow reactor, 220 degrees C, atmospheric pressure) and for the hydrodesulfurization of dibenzothiophene (flow reactor, 340 degrees C, 3 MPa hydrogen pressure). The addition of the promoter increased significantly the activity of the Mo/alumina catalyst for both reactions, up to a maximum obtained with the catalysts having a (promoter)/(promoter+Mo) molar ratio equal to 0.3-0.4. This increase in activity was due in part to an increase in the hydrogenating properties of the Mo/alumina catalyst. However, an additional modification of the catalyst (basic and nucleophilic properties) must be considered to account for the spectacular effect of the promoter on the rate of the dibenzothiophene direct desulfurization reaction. (C) 1999 Elsevier Science B.V. All rights reserved.

Cobalt boride catalysts for hydrogen generation from alkaline NaBH4 solution

Cobalt boride precursors were synthesized via chemical reaction of aqueous sodium borohydride with cobalt chloride, and followed by heat-treating at various temperatures. The as-prepared Co-B catalysts were characterized and analyzed by X-ray diffraction (XRD), nitrogen adsorption-desorption and catalytic activity test; and were adopted to help accelerating hydrolysis reaction of NaBH4 alkaline solution. The Co-B catalyst treated at 500 degrees C exhibits the best catalytic activity, and achieves an average H, generation rate of 2970 ml/min/g, which may give a successive H, supply for a 481 W proton exchange membrane fuel cell (PEMFC) at 100% H-2 utilization. (c) 2005 Elsevier B.V. All rights reserved.

The formation of Co2C species in activated carbon supported cobalt-based catalysts and its impact on Fischer-Tropsch reaction

The cobalt carbide (Co2C) species was formed in some activated carbon supported cobalt-based (Co/AC) catalysts during the activation of catalysts. It was found that the activity of Fischer-Tropsch reaction over Co-based catalysts decreased due to the formation of cobalt carbide species. Some promoters and pretreatment of activated carbon with steam could restrain the formation of cobalt carbide.