Anatomia foliar com ênfase nos tricomas secretores e análise cromatográfica do óleo essencial de Melissa officinalis L. (Lamiaceae)

Adult leaves of Melissa officinalis L. (Lamiaceae) harvested during the time of budding had been used in the study of the leaf anatomy in the identification of the secretory structures. The material was fixed in FAA 50%, dehydrated in alcoholic series (ethyl), infiltrated in paraffin, sectioned at 13 mm, staining and later analyzed through optic microscopy. Also it was carried through a study in gaseous chromatography for attainment of the rude essential oil. Analyses of transversal sections of the leaf of Melissa officinalis, has identified the presence of two types of trichomes secretory: peltate and capitate, beyond the presence of trichome tector. Trichome capitate, identified in literature as (type I), presents variations in its morphology in relation to the cells number stalk and the head secretory cells number. The chromatographic analysis of the essential oil identified the presence of monoterpenes, in two major components, responsible for more than 87.8% of the relative composition in the rude oil, beyond sesquiterpenes in smaller proportions.

Papel da eritropoetina recombinante humana sobre o metabolismo dos carboidratos, paratormônio, cálcio iônico, zinco, prolactina e níveis pressóricos em renais crônicos tratados com hemodiálise

Objective: To evaluate the influence of recombinant human erythropoietin (Epho) on carbohydrate metabolism, parathyroid hormone, calcium ionic, zinc, prolactin and blood pressure (BP) in chronic renal failure treated by hemodialysis. Methods: Ten patients in hemodialysis were followed during 24 weeks in two phases: 12 weeks pre-Epho (BP was measured pre and post hemodialysis sessions) and 12 weeks post-Epho (BP was measured as above and also the blood levels of glucose, insulin, parathyroid hormone, calcium ionic, prolactin, and zinc). Results: Patients were 39.8±8.5 y, 50% males. Hematocrit and hemoglobin presented a significant increase four weeks after Epho (22.3±2.3 to 28.1±2.6% and 7.4±0.8 to 9.4±0.9 g/dL, p<0.05). BP (mmHg) and weight pre-Epho: 158±99 and 59±13 (before hemodialysis), 147±96 and 55±13 (after hemo) and post-Epho: 161±100 and 59±13 (before hemo) 155±101 and 56±12 (after hemo) were all not statistically different in any moment. There are also no difference pre and post-Epho in fast glucose (91.8±6.5 and 90.8±6.1 mg/dL, p>0.05), parathyroid hormone (341.4±249.3 and 515.7±310 pg/ mL), calcium ionic (3,66±0.63 and 3.76±0.45 mmol/L), prolactin (males: 327±144.1 and 298.1 ±145.2 μg/mL; females: 666.2±426.6 and 659±395.3 μg/mL) and zinc (median of 0.73 and 0.71 μg\L). Basal insulin was lower after Epho (median of 9.1 to 3.8 μg/mL, p<0.05). Conclusion: These data suggest that recombinant human erythropoietin was effective to improve the anemia and the carbohydrate metabolism in patients with chronic renal failure treated by hemodialysis. © Copyright Moreira Jr. Editors. Todos os direitos reservados.

Anatomia do músculo abdutor crural caudal do gato doméstico (Felis catus domesticus, Linnaeus 1758)

The frequency and the morphology of the abductor cruris caudalis muscle were studied in 58 adult unknown breed cats. The sample included 36 females and 22 males. After the dissection, were found a frequency for the presence of the same muscle of 98.3%. The abductor cruris caudalis muscle appears symmetrically in 35 females (97.2%) and 22 males (100.0%). When present, it were originated on the transversal process of the 1st vertebrae caudales, in the glutea region, below the gluteus superficialis muscle, throughing almost all the thigh under biceps femoris muscle, where it make a parallel relation with the sciatic nerve, receiving nervous ramifications of the same in 78.3% of the cases. The medium found for the width and thickness of his muscle venter was respectively of 2.03 mm and 0.35 mm. Its insertion occurs on the biceps femoris muscles approximate to the fascia cruris, where both finish fusing theirselves.

Anatomia do nervo isquiático em mocós (Kerodon rupestris WIED, 1820) aplicada a clínica de animais silvestres

To know the origin of the ischiatic nerve in mocos (Kerodon rupestris Wied,1820) near by intervertebral forames and the muscling belonging to its routes were used 10 adult animals, from CEMAS-ESAM. After natural obit, they were fixed in formol (10%) and dissected to exposition and to singt of the ischiatic nerve. The results were indicated in percentage. Variations in the quantity of the lumber and sacral vertebras nere observed, five animals (50,00%) reveled seven lumbar vertebras and three sacral ones; two animals recrealed seven lumbar vertebras and four sacral ones, and two animals reveled six lumbar vertebras and three sacral ones. An animal (10,00%) revealed six lumbar vertebras and four ones. Therefore, the origin of the nerve was differentiated five animals (50,00%) had the participation of L 7,S 1,S 2; two animals (20,00%) with L 7,S 1; and a little part of S 2. Two animals (20,00%) with L 6,S 1,S 2, and an animal (10,00%) with L 6,S 1, and a little part of S 2. The last root of the ischiatic nerve in all its origins, contribute to the constitution of the first root of pudental nerve. It was verified that in all its route, the ischiatic nerves (100,00%) ceded branches to the muscles: medial gluteus, deep gluteus, superficial gluteus, emiting muscular branches to the femoral biceps or to thigh, and to the semimembranous and semi-tendinous muscles, that is continuous with a high calibre trunk, originating the fibular nerve(sideways), the tibial nerve(medial) and the lateral plantar sural cutaneous nerve (caudal).

Morfo-anatomia do eixo vegetativo aéreo de Achyrocline alata (Kunth) DC. (Asteraceae)

Achyrocline alata occurs in a dense and low vegetation in Mato Grosso do Sul State, Brazil and it has been studied under physical-chemical and yield aspects, being scarce anatomical data. The present work has as objective the study of the aerial vegetative axis. Stem is cylindrical and hairy, having five wings, which are arisen from their leaves. The blade shows the presence of uniseriate epidermis, which is covered by the cuticle of variable thickness; and non-glandular and glandular trichomes. The non-glandular trichomes are uniseriate and multicellular and have their apex cell in whip form, while the glandular trichomes are multicellular, uniseriate or biseriate. The mesophyll is dorsiventral with uni-stratified palisade parenchyma in most of the cases and lacunal parenchyma formed by three to four layers of irregular cells. Only one collateral vascular bundle occurs in the midvein. Stem in transversal section is covered by epidermis with trichomes similar to leaves; the cortex is constituted by a discontinuous area of angular collenchyma, which is followed by chlorophyll parenchyma. Vascular cylinder that is delimited by pericycle shows vascular bundles of collateral type. In the secondary structure, the periderm is originated from epidermal and subepidermal tissues. In vascular region, the fascicular cambium differs into secondary xylem and phloem, while interfascicular cambium produces sclerenchymatous tissue.

Diferenciação hepatocítica de células-tronco mesenquimais da medula óssea humana

Some recent articles have reported that mesenchymal stem cells (MSCs) can be induced to express hepatocyte markers by transplanting them into animal models of liver damage, or by in vitro culture with growth factors and cytokines. In this study, the aim is to evaluate the behavior of MSCs subjected to induction of hepatocyte differentiation. The MSCs were isolated from the bone marrow of 4 normal donors, characterized and subjected to both in vitro and in vivo induction of hepatocyte differentiation. The in vitro induced cells showed morphological changes, acquiring hepatocyte-like features. However, the immunophenotype of these cells was not modified. The induced cells exhibited no increase in albumin, cytokeratin 18 or cytokeratin 19 transcripts, when analyzed by real-time RT-PCR. The expression of albumin, cytokeratin 18 and alpha fetoprotein was also unchanged, according to immunofluorescence tests. In vivo, the MSC demonstrated a potential to migrate to damaged liver tissue in immunodeficient mice. Taken together, the results suggest that bone marrow MSCs are incapable of in vitro differentiation into hepatocytes by the approach used here, but are capable of homing to damaged hepatic tissue in vivo, suggesting a role for them in the repair of the liver. This contribution to tissue repair could be associated with a paracrine effect exerted by these cells.

Condiciones sociales de la reproduccion humana: un proyecto de investigacion-accion, Brasil

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Aspectos metodológicos y sociológicos de la fecundidad humana

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Bio-estadística de la reproducción humana

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La sociología de la fecundidad humana: tendencias actuales de la investigación y bibliografía

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Aspectos biométricos de la fecundidad humana

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Seminario sobre bioestadisticas de la reproduccion humana; sesiones de trabajo

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Anatomia radicial de Mormodes sinuata Rchb. f. & Warm.; (Catasetinae, Cymbidieae: Orchidaceae)

Since there is a lack of information on the root organization of the sub-tribe Catasetinae, current research describes the anatomy of Mormodes sinuata roots and indicate adaptations related to water provision and other taxonomical traits. Five roots of three plants, cut at the median region with a blade, were fixed and preserved in alcohol 5%. Cuts were stained by saffron blue 0.05% and mounted in glycerin. Although the roots have structural characteristics adapted to epiphytism, some anatomic characteristics demonstrate that the species requires more watering or wetter environments when compared with other types of Catasetinae. Root anatomic characteristics foreground results by authors who include the sub-tribe Catasetinae within the tribe Cymbidieae. Further, anatomic characteristics have a generic uniformity and are a tool for the genus's systematics.

Anatomia topográfica e comportamento arterial do pâncreas em cágados Trachemys scripta elegans (wied, 1838)

The turtle Trachemys scripta elegans popularly known as American tiger water are native regions winged Florida and Mississippi, in the United States. We used twenty (20) turtles (Trachemys scripta elegans), adult males and females, which were euthanized under Resolution 714 of June 20, 2002 the Federal Council of Veterinary Medicine (CFMV). After euthanasia were identified aortas right and left to the injection of Neoprene latex 450, stained with specific pigment. To obtain the vinyl mold aorta was injected through the right and left vinyl acetate, followed by corrosion in sulfuric acid. It was observed that the pancreas is closely related to the liver, gizzard, gall bladder, and duodenum. His face cranial this distal region of the pylorus, while its caudal along the cranial region of the duodenum. Anatomically, the pancreas is an elongated body structure featuring a lightly lobed. As to the pancreas arterial vasculature is flushed in its transverse plane of two arteries arising from the celiac artery, and each antimere two pancreaticoduodenal artery in the cranial region, close to the pylorus by the pancreaticoduodenal artery flow in the caudal portion along the duodenum.