First report of Angiostrongylus cantonensis (Nematoda: Metastrongylidae) in Achatina fulica (Mollusca: Gastropoda) from Southeast and South Brazil

The rat lungworm Angiostrongylus cantonensis is a worldwide-distributed zoonotic nematode that can cause human eosinophilic meningoencephalitis. Here, for the first time, we report the isolation of A. cantonensis from Achatina fulica from two Brazilian states: Rio de Janeiro (specifically the municipalities of Barra do Piraí, situated at the Paraiba River Valley region and São Gonçalo, situated at the edge of Guanabara Bay) and Santa Catarina (in municipality of Joinville). The lungworms were identified by comparing morphological and morphometrical data obtained from adult worms to values obtained from experimental infections of A. cantonensis from Pernambuco, Brazil, and Akita, Japan. Only a few minor morphological differences that were determined to represent intra-specific variation were observed. This report of A. cantonensis in South and Southeast Brazil, together with the recent report of the zoonosis and parasite-infected molluscs in Northeast Brazil, provide evidence of the wide distribution of A. cantonensis in the country. The need for efforts to better understand the role of A. fulica in the transmission of meningoencephalitis in Brazil and the surveillance of molluscs and rodents, particularly in ports, is emphasized.

Ecoauditoria de l’IES Santa Coloma de Farners

El projecte “Ecoauditoria de l’IES Santa Coloma de Farners” consisteix en: la diagnosi ambiental del centre, les propostes de les línees d'acció, les propostes d’ actuació, la valoració i anàlisi de les propostes i l’ autoseguiment del pla d’ acció indispensables per a que el centre pugui aconseguir el distintiu d'Escola Verda que atorga la Generalitat de Catalunya. Incorporar l’ educació ambiental en el currículum escolar és un altre dels objectius d’ aquest treball. Així que es proposen activitats curriculars amb l’ objectiu de transmetre una major sensibilització ambiental a l’alumnat i a tota la comunitat educativa en general

Spotted fever group Rickettsia infecting ticks (Acari: Ixodidae) in the state of Santa Catarina, Brazil

During 2006-2008, a total of 260 adult ticks were collected from domestic and wild animals in different regions of the state of Santa Catarina (SC), Brazil, including areas where human cases of Brazilian spotted fever have been reported. Collected ticks belonging to nine species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Amblyomma longirostre, Amblyomma ovale, Amblyomma tigrinum, Dermacentor nitens, Rhipicephalus microplus and Rhipicephalus sanguineus) were tested by polymerase chain reaction (PCR) for rickettsial infection. Overall, eight (3.1%) ticks were found to be infected with Rickettsia species. After sequencing the PCR products, we determined that the sequences generated from three A. aureolatum, one A. ovale and one R. sanguineus from the municipality of Blumenau, one A. ovale from the municipality of Águas Mornas and one A. ovale from the municipality of Urussanga were identical to the corresponding partial rickettsial ompA gene sequence of Rickettsia parkeri strain Atlantic rainforest. The sequence generated from one A. longirostre from Blumenau was 100% identical to the corresponding partial rickettsial ompA gene sequence of Rickettsia amblyommii strain AL. Because R. parkeri strain Atlantic rainforest was recently shown to have caused two cases of human spotted fever in other states of Brazil, the role of this rickettsial agent as a possible etiological agent of spotted fever in SC is discussed.

Biological, biochemical and molecular features of Trypanosoma cruzi strains isolated from patients infected through oral transmission during a 2005 outbreak in the state of Santa Catarina, Brazil: its correspondence with the new T. cruzi Taxonomy Consensus (2009)

We examined strains of Trypanosoma cruzi isolated from patients with acute Chagas disease that had been acquired by oral transmission in the state of Santa Catarina, Brazil (2005) and two isolates that had been obtained from a marsupial (Didelphis aurita) and a vector (Triatoma tibiamaculata). These strains were characterised through their biological behaviour and isoenzymic profiles and genotyped according to the new Taxonomy Consensus (2009) based on the discrete typing unities, that is, T. cruzi genotypes I-VI. All strains exhibited the biological behaviour of biodeme type II. In six isolates, late peaks of parasitaemia, beyond the 20th day, suggested a double infection with biodemes II + III. Isoenzymes revealed Z2 or mixed Z1 and Z2 profiles. Genotyping was performed using three polymorphic genes (cytochrome oxidase II, spliced leader intergenic region and 24Sα rRNA) and the restriction fragment length polymorphism of the kDNA minicircles. Based on these markers, all but four isolates were characterised as T. cruzi II genotypes. Four mixed populations were identified: SC90, SC93 and SC97 (T. cruzi I + T. cruzi II) and SC95 (T. cruzi I + T. cruzi VI). Comparison of the results obtained by different methods was essential for the correct identification of the mixed populations and major lineages involved indicating that characterisation by different methods can provide new insights into the relationship between phenotypic and genotypic aspects of parasite behaviour.

Serological detection of St. Louis encephalitis virus and West Nile virus in equines from Santa Fe, Argentina

St. Louis encephalitis virus (SLEV) and West Nile virus (WNV) present ecological and antigenic similarities and are responsible for serious human diseases. In addition, WNV is a significant pathogen in terms of equine health. The purpose of our study was to analyse the seroprevalence of SLEV and WNV in equine sera collected in Santa Fe Province, Argentina. The seroprevalence determined using the plaque reduction neutralisation test was 12.2% for SLEV, 16.2% for WNV and 48.6% for a combination of both viruses. These results provide evidence of the co-circulation of SLEV and WNV in equines in Santa Fe.

Detection of the B"-GWGR variant in the southernmost region of Brazil: unveiling the complexity of the human immunodeficiency virus-1 subtype B epidemic

Typical human immunodeficiency virus-1 subtype B (HIV-1B) sequences present a GPGR signature at the tip of the variable region 3 (V3) loop; however, unusual motifs harbouring a GWGR signature have also been isolated. Although epidemiological studies have detected this variant in approximately 17-50% of the total infections in Brazil, the prevalence of B"-GWGR in the southernmost region of Brazil is not yet clear. This study aimed to investigate the C2-V3 molecular diversity of the HIV-1B epidemic in southernmost Brazil. HIV-1 seropositive patients were ana-lysed at two distinct time points in the state of Rio Grande do Sul (RS98 and RS08) and at one time point in the state of Santa Catarina (SC08). Phylogenetic analysis classified 46 individuals in the RS98 group as HIV-1B and their molecular signatures were as follows: 26% B"-GWGR, 54% B-GPGR and 20% other motifs. In the RS08 group, HIV-1B was present in 32 samples: 22% B"-GWGR, 59% B-GPGR and 19% other motifs. In the SC08 group, 32 HIV-1B samples were found: 28% B"-GWGR, 59% B-GPGR and 13% other motifs. No association could be established between the HIV-1B V3 signatures and exposure categories in the HIV-1B epidemic in RS. However, B-GPGR seemed to be related to heterosexual individuals in the SC08 group. Our results suggest that the established B"-GWGR epidemics in both cities have similar patterns, which is likely due to their geographical proximity and cultural relationship.

L'Hospital de Sant Jaume i Santa Magdalena de Mataró, una institució al servei de la ciutat des del segle XVII

Estudi que repassa la història de l'edifici de l'Hospital de Sant Jaume i Santa Magdalena de Mataró i de la seva institució, i anàlisi de com ha influït en el seu entorn social.

Diagnosi multifuncional de la conca de la riera de Santa Coloma

Estudi de la qualitat de l’aigua de la riera de Santa Coloma i el seu paper dins la connectivitat multifuncional de la comarca de la Selva. La riera és l’eix fluvial principal de la subconca més important de la Tordera i el seu curs abraça gran part de la comarca. Per avaluar la qualitat de l’aigua s’han fet anàlisis amb paràmetres fisicoquímics i biològics. Per a l’estudi de la connectivitat s’ha fet un anàlisi a nivell local (barreres transversals a la llera i QBR) i a nivell regional identificant les principals amenaces per a la connectivitat ecològica i paisatgística (col.lisions d’ungulats o punts negres, fitxes de punts crítics, entre d’altres). S’arriba a la conclusió que la riera no té una bona qualitat de l’aigua (en alguns casos hi ha eutrofització), el seu bosc de ribera està molt degradat (sobretot a la part baixa del curs) i finalment, hi ha un gran nombre d’espais amb una gran importància estratègica per a la connectivitat però que alhora pateixen fortes pressions antròpiques per les infraestructures lineals, les quals a més, redueixen la permeabilització ecològica i fins i tot, provoquen col.lisions amb ungulats

Ciclo de vida organizacional pautado no modelo de Lester, Parnell e Carraher (2003) e na lógica fuzzy: classificação de empresas de um segmento industrial de Santa Catarina

Neste estudo, objetiva-se identificar os estágios do ciclo de vida organizacional pautados no modelo de Lester, Parnell e Carraher (2003) das empresas do segmento industrial de máquinas, aparelhos e materiais elétricos do estado de Santa Catarina. Pesquisa descritiva, com abordagem quantitativa, foi realizada por meio de levantamento com aplicação de questionário aos gestores das empresas. A população constituiu-se das 264 empresas desse segmento econômico, listadas na Secretaria da Fazenda do Estado de Santa Catarina, e a amostra não aleatória das 40 empresas que responderam a pesquisa. As variáveis de identificação dos estágios de ciclo de vida utilizadas no questionário foram extraídas de Lester, Parnell e Carraher (2003). Os dados da pesquisa foram submetidos à técnica estatística denominada lógica fuzzy. Os resultados da pesquisa demonstraram que 57,5% das empresas foram classificadas no estágio do nascimento, 15% do, 7,5% da Maturidade, 10% do rejuvenescimento e 10% do declínio. Concluiu-se que determinados estágios do ciclo de vida organizacional estão próximos uns dos outros e que não se pode perceber claramente uma progressão determinista nas fases do ciclo de vida, como uma sequência única, definitiva e irreversível, no sentido tradicional biológico.

L'Església de Santa Helena de Rodes: recerca arqueològica i restauració arquitectònica

Descripció de l’estudi arqueològic i la restauració arquitectònica de l’Església de Santa Helena o Santa Creu de Rodes realitzats a principis dels anys 90

Cirugía micrográfica de Mohs: Estudio retrospectivo de los últimos trece años (1999-2011) en el Hospital de la Santa Creu i Sant Pau

El objetivo de este trabajo fue describir las características de los pacientes tratados con cirugía micrográfica de Mohs entre 1999-2011 y evaluar la eficacia de esta cirugía para el tratamiento de los carcinomas basocelulares. Durante este periodo se realizaron 560 cirugías de Mohs, el 95.36% de las cuales fueron de carcinomas basocelulares. Se observó una tasa de recidiva del 1.40% para los carcinomas basocelulares primarios y del 13.62% para los recurrentes, siendo la diferencia estadísticamente significativa (p <0.0001).

Els Barris de Sant Narcís i de Santa Eugènia davant les avingudes del Güell

Les obres d’acondicionament i millora del riu Güell passen per dues fases. La primera, fins al 1971, demostren que el problema no estava ni de bon tros solucionat, més fruit de la improvisació i poca lògica de l’Administració que d'una bona política de planificació hidràulica. Les obres mes recents, (transvasament Güell-Marroc.Massanas-Ter), aparentment solucionen el problema. Però cal insistir un cop més; d'ençà el 1970 i fins avui dia, el riu Güell no ha sofert cap més avinguda important, si bé és cert que Girona no n'ha patit cap d’aquelles dimensions. Com passa en la majoria dels casos, el risc d'avingudes és un fantasma que ve de la mateixa manera que se'n va, que dura l’escàs període de l'avinguda i els dies-mesos que segueixen

A saúde do trabalhador na concepção de acadêmicos de enfermagem

Neste estudo, de abordagem qualitativa, buscamos compreender a concepção do acadêmico de enfermagem do Curso de Enfermagem da Universidade Federal de Santa Catarina sobre saúde do trabalhador. Foram feitas três questões a vinte acadêmicos: O que entende por saúde do trabalhador? De que forma a enfermagem pode contribuir para a saúde do trabalhador? Qual a relação que existe entre trabalho e estilo de vida? Os resultados foram agrupados nos temas: concepções sobre saúde do trabalhador; papel da enfermagem no contexto da saúde do trabalhador; relação entre trabalho e estilo de vida. Destacamos a interrelação entre trabalho e estilo de vida, e trabalho e saúde do trabalhador. As reflexões realizadas reforçam a necessidade dessa temática ser discutida nas universidades, para que os acadêmicos sejam estimulados a pensar sobre sua saúde desde o início da academia. Assim estimulados, acreditamos que possam incorporar ações de proteção voltadas a sua saúde no trabalho.