Integração básico-clínica no internato médico: Psiquiatria e Farmacologia

This study quantifies fifth year medical students' knowledge after their participation in a basic clinical integration activity of psychiatry and pharmacology and investigates their opinions on the importance of the activity. A quali-quantitative study was performed using pre- and post-test assessment (multiple choice) and linguistic content analysis using the Discourse of the Collective Subject (DCS) technique. A significant increase in the percentage of correct answers in the post-test assessment was found compared to pre-test (p <0.001). The central ideas obtained from the DCS content analysis were: very relevant activity; expansion of the activity to other internships; difficulty in basic disciplines; application of cognitive assessment; and teacher's active participation in discussions. These results suggest that integration between pharmacology and the practical intern activities in psychiatry allowed pharmacological concepts to be broadened, rendering the learning meaningful. Although students identified the need for better organization of the activity, its the level of acceptance was verbalised through the suggestion that it could be extended to the other fifth year internships.

Coberturas vegetais e doses de nitrogênio em trigo sob sistema plantio direto

The application of conservation practices in production systems is essential to the sustainability of the agricultural production capacity. The use of different cover crops can reduce the need of topdressing nitrogen fertilizers in wheat crops. This study aimed at evaluating the influence of cover crops residues (millet, sunn hemp, pigeon pea, millet + sunn hemp, millet + pigeon pea and fallow ground), grown previously to the summer crop (rice), and topdressing nitrogen doses (0 kg ha-1, 25 kg ha-1, 50 kg ha-1, 75 kg ha-1, 100 kg ha-1 and 125 kg ha-1), in the development and yield of wheat under no-tillage system. The experiment was carried out in Selvíria, Mato Grosso do Sul State, Brazil, in a Red Oxisol, in 2009/2010 and 2010/2011. The experimental design was randomized blocks, in a 6x6 factorial scheme. The cover crops developed during August/October (2009/2010) and September/November (2010/2011), previously to the summer crop, with the chemical desiccation performed respectively at 73 and 55 days after sowing. The wheat (winter) was sown in May, in both periods, and harvested at 113 (2009/2010) and 106 (2010/2011) days after emergence. The cover crops residues of pigeon pea, sunn hemp and millet + sunn hemp, preceding wheat, provided higher wheat yields, relatively to the fallow ground. The wheat yield showed a quadratic response to the increase in the nitrogen availability, reaching higher yields with the nitrogen doses estimated in 113 kg ha-1 (2010) and 98 kg ha-1 (2011).

Revendo estudos sobre a assistência domiciliar ao idoso

Home care presents itself as a new modality of care for the elderly, especially in Brazil , and has been inserted into public policies. This study aims to identify scientific productions about home care, in order to ascertain which are the contributions of this social dynamic in the elderly. The research was conducted in the databases MEDLINE and LILACS, with articles published between the years 2001- 2011, in Portuguese and English. The combination of keywords used was home care and elderly , elderly and care, and elderly and caregiver, and correspondents in Portuguese. The discussion highlighted the importance of home care, especially for lonely or neglected elderly. The review revealed few studies concerning the social dynamics in the home care of the elderly and the need to broaden the discussion beyond the field of health.

Proteinograma sérico, com ênfase em proteínas de fase aguda, de bovinos sadios e bovinos portadores de enfermidade aguda de ocorrência natural

Nas últimas décadas, as proteínas de fase aguda (PFAs) tornaram-se biomarcadores de escolha em medicina humana para identificação e monitoração de doenças. Não há razão para imaginar que tais pesquisas clínicas não sejam igualmente úteis na medicina veterinária. Com o objetivo de verificar a importância das PFAs como biomarcadores de doenças inflamatórias em bovinos, determinou-se o proteinograma sérico, por meio da técnica de eletroforese SDS-PAGE, com interesse especial nas PFAs. Foram utilizados 30 animais, distribuídos em dois grupos: 15 bovinos sadios e 15 bovinos doentes (cinco com mastite estafilocócica, cinco com fotossensibilização e cinco com onfaloflebite). Os animais foram submetidos a colheitas diárias de sangue durante sete dias, enquanto internados no Hospital Veterinário da Unesp, Campus de Jaboticabal. Ceruloplasmina e haptoglobina apresentaram elevação significativa em animais acometidos por mastite, fotossensibilização e onfaloflebite (275,17% e 343,71%; 175,17% e 230,19%; 114,47% e 144,47%, respectivamente). A α1-glicoproteína ácida foi um bom biomarcador apenas em animais com mastite e fotossensibilização, elevando, respectivamente, suas concentrações séricas em 198,14% e 145,89%. Fibrinogênio mostrou-se um indicador confiável apenas em bovinos com mastite, com elevação de 146,5% em relação ao grupo sadio. Ficou clara a diferença na responsividade de distintas PFAs frente a diferentes estímulos inflamatórios. Ceruloplasmina e haptoglobina foram biomarcadores mais sensíveis e, portanto, mais confiáveis entre as PFAs estudadas nessa espécie.

Descrição do potencial de retenção de nitrogênio em frangas de postura por diferentes metodologias: máxima deposição e estimativas da ingestão de metionina+cistina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A nonsynonymous mutation at HLA-E defines the new E*01:06 allele in Brazilian individuals

We report a novel nonclassical class I HLA-E*01:06 allele observed in Brazilian individuals.

Enteral nutrition feeding alters antioxidant activity in unstimulated whole saliva composition of patients with neurological disorders

Patients with neurological disorders have an increased risk of oral and systemic diseases due to compromised oral hygiene. If patients lose the ability to swallow and chew food as a result of their disorder, enteral nutrition is often utilized. However, this type of feeding may modify salivary antioxidant defenses, resulting in increased oxidative damage and the emergence of various diseases. The aim of this study was to evaluate the effects of enteral nutrition on biochemical parameters in the unstimulated whole saliva composition of patients with neurological disorders. For this, enzymatic (superoxide dismutase - SOD; glutathione peroxidase - GPx) and non-enzymatic (uric acid; ferric ion reducing antioxidant power - FRAP) antioxidant activity, as well as a marker for oxidative damage (thiobarbituric acid reactive substances - TBARS) were analyzed. Unstimulated whole saliva was collected from 12 patients with neurological disorders and tube-feeding (tube-fed group - TFG), 15 patients with neurological disorders and normal feeding via the mouth (non-tube-fed group - NTFG), and 12 volunteers without neurological disorders (control group - CG). The daily oral hygiene procedures of TFG and NTFG patients were similar and dental care was provided monthly by the same institution's dentist. All patients exhibited adequate oral health conditions. The salivary levels of FRAP, uric acid, SOD, GPx, TBARS, and total protein were compared between studied groups. FRAP was increased (p < 0.05) in the NTFG (4651 +/- 192.5 mmol/mL) and the TFG (4743 +/- 116.7 mmol/mL) when compared with the CG (1844 +/- 343.8 mmol/mL). GPx values were lower (p < 0.05) in the NTGF (8.24 +/- 1.09 mmol/min/mg) and the TFG (8.37 +/- 1.60 mmol/min/mg) than in the CG (15.30 +/- 2.61 mmol/min/mg). Uric acid in the TFG (1.57 +/- 0.23 mg/dL) was significantly lower than in the NTFG (2.34 +/- 0.20 mg/dL) and the CG (3.49 +/- 0.21 mg/dL). Protein was significantly lower in the TFG (5.35 +/- 0.27 g/dL) than in the NTFG (7.22 +/- 0.57 g/dL) and the CG (7.86 +/- 0.54 g/dL). There was no difference in the salivary flow rate and SOD between groups. Enteral nutrition in patients with neurological disorders was associated with lower oxidative damage, resulting in increased salivary. antioxidant capacity. These results emphasize the importance of oral care for this population to prevent oral and systemic diseases. (C) 2014 Elsevier Ltd. All rights reserved.

Transmissable Dog Cancer Genome Reveals the Origin and History of an Ancient Cell Lineage

Canine transmissible venereal tumor (CTVT) is the oldest known somatic cell lineage. It is a transmissible cancer that propagates naturally in dogs. We sequenced the genomes of two CTVT tumors and found that CTVT has acquired 1.9 million somatic substitution mutations and bears evidence of exposure to ultraviolet light. CTVT is remarkably stable and lacks subclonal heterogeneity despite thousands of rearrangements, copy-number changes, and retrotransposon insertions. More than 10,000 genes carry nonsynonymous variants, and 646 genes have been lost. CTVT first arose in a dog with low genomic heterozygosity that may have lived about 11,000 years ago. The cancer spawned by this individual dispersed across continents about 500 years ago. Our results provide a genetic identikit of an ancient dog and demonstrate the robustness of mammalian somatic cells to survive for millennia despite a massive mutation burden.

Aspectos biológicos e técnica de criação do gorgulho-da-goiaba, Conotrachelus psidii marshall (Coleoptera: Curculionidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Growth in weight and of some tissues in the bullfrog: fitting nonlinear models during the fattening phase

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação de atributos de Latossolo Bruno e de Terra Bruna Estruturada da região de Guarapuava, Paraná, por meio de sua energia refletida

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sieved para-orthogonal polynomials on the unit circle

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Practising Arithmetic Using Educational Video Games with an Interpersonal Computer

Studies show the positive effects that video games can have on student performance and attitude towards learning. In the past few years, strategies have been generated to optimize the use of technological resources with the aim of facilitating widespread adoption of technology in the classroom. Given its low acquisition and maintenance costs, the interpersonal computer allows individual interaction and simultaneous learning with large groups of students. The purpose of this work was to compare arithmetical knowledge acquired by third-grade students through the use of game-based activities and non-game-based activities using an interpersonal computer, with knowledge acquired through the use of traditional paper-and-pencil activities, and to analyze their impact in various socio-cultural contexts. To do this, a quasi-experimental study was conducted with 271 students in three different countries (Brazil, Chile, and Costa Rica), in both rural and urban schools. A set of educational games for practising arithmetic was developed and tested in six schools within these three countries. Results show that there were no significant differences (ANCOVA) in the learning acquired from game-based vs. non-game-based activities. However, both showed a significant difference when compared with the traditional method. Additionally, both groups using the interpersonal computer showed higher levels of student interest than the traditional method group, and these technological methods were seen to be especially effective in increasing learning among weaker students.

Avaliação da indução de apoptose pela desintegrina recombinante DisBa-01 em macrófagos e células tumorais

O câncer é causado pela proliferação descontrolada de células, demonstrando uma capacidade coletiva de invasão e metástase. Durante a metástase, as células malignas precisam resistir a anoikis, uma apoptose celular gerada por falta de adesão. No câncer, as integrinas influenciam as células do hospedeiro associadas ao tumor bem como as próprias células tumorais, tendo o potencial de modular a progressão tumoral, a sobrevivência celular, a invasão e a metástase. A integrina αvβ3 é expressa em diversos tumores humanos, mas está em níveis muito reduzidos ou mesmo ausente nos tecidos normais, sendo considerada um alvo privilegiado na terapia anti-tumoral. Células derivadas da medula óssea, como o monócito/macrófago, também expressam esta integrina, ainda que em níveis reduzidos. A desintegrina recombinante DisBa-01 atua principalmente sobre as integrinas αvβ3 e αIIbβ3, e parece possuir a capacidade de inibir a adesão celular de linhagens possuindo a integrina αvβ3 à vitronectina. Sendo assim, observou-se a apoptose que pode ser gerada pela inibição da adesão celular ou pela própria presença da desintegrina, um antagonista de integrina que afeta a adesão celular, em linhagem neoplásica e em linhagem imortalizada de macrófagos, utilizando os métodos de marcação com anexina V e técnica de TUNEL, bem como a presença de células viáveis através da técnica de MTT. As linhagens celulares utilizadas nesse experimento foram expostas a desintegrina DisBa-01 por 24 horas para realização dos testes e as populações celulares aderentes e não aderentes foram analisadas separadamente. Verificou-se uma diminuição na viabilidade celular quando ocorre perda de adesão e na presença da proteína, mas foi observado um resultado não significativo para a ocorrência de apoptose. A desintegrina DisBa-01 ocasiona uma diminuição na viabilidade celular, entretanto parece não ser por apoptose gerando anoikis

A criança de 01 a 03 anos: uma reflexão sobre a evolução da imitação no processo de formação do eu

This investigation aims to observe play contents and elements that make it possible to notice if a child is at the imitation stage. First of all, this study reviews Piaget’s findings about the origins of imitation, and how it develops into representation. After that, “game” is analyzed in order to understand the cognitive representation and how children develop the ability to use symbols. Based on these assumptions, a study was developed into a public school (Profª Maria Teixeira Fittipaldi) with 9 children, aged between 1 to 3. Methodology included the observation of play and activity time; a diary to record the observations; and the analysis of the collected data, based on Piaget’s research into the classification and development of symbolic play. The results show that all observed situations were at the “first stage”, which reveal the apogee of ludic symbolism, and that the kind of game varied according to the child’s age. Moreover, it was possible to notice the importance of symbolic play at preschool, since it triggers world knowledge development and helps interpersonal relationship growth.