Immunohistochemical evidence of synaptic retraction, cytoarchitectural remodeling, and cell death in the inner retina of the rat model of Oygen-Induced Retinopathy (OIR)

Purpose. Postnatal exposure to hyperoxia destroys the plexiform layers of the neonatal rat retina, resulting in significant electroretinographic anomalies. The purpose of this study was to identify the mechanisms at the origin of this loss. Methods. Sprague-Dawley (SD) and Long Evans (LE) rats were exposed to hyperoxia from birth to postnatal day (P) 6 or P14 and from P6 to P14, after which rats were euthanatized at P6, P14, or P60. Results. At P60, synaptophysin staining confirmed the lack of functional synaptic terminals in SD (outer plexiform layer [OPL]) and LE (OPL and inner plexiform layer [IPL]) rats. Uneven staining of ON-bipolar cell terminals with mGluR6 suggests that their loss could play a role in OPL thinning. Protein kinase C(PKC)-α and recoverin (rod and cone ON-bipolar cells, respectively) showed a lack of dendritic terminals in the OPL with disorganized axonal projections in the IPL. Although photoreceptor nuclei appeared intact, a decrease in bassoon staining (synaptic ribbon terminals) suggests limited communication to the inner retina. Findings were significantly more pronounced in LE rats. An increase in TUNEL-positive cells was observed in LE (inner nuclear layer [INL] and outer nuclear layer [ONL]) and SD (INL) rats after P0 to P14 exposure (425.3%, 102.2%, and 146.3% greater than control, respectively [P < 0.05]). Conclusions. Results suggest that cell death and synaptic retraction are at the root of OPL thinning. Increased TUNEL-positive cells in the INL confirm that cells die, at least in part, because of apoptosis. These findings propose a previously undescribed mechanism of cell death and synaptic retraction that are likely at the origin of the functional consequences of hyperoxia.

Phosphorylated α-synuclein-immunoreactive retinal neuronal elements in Parkinson's disease subjects

Visual symptoms are relatively common in Parkinson's disease (PD) and optical coherence tomography has indicated possible retinal thinning. Accumulation of aggregated α-synuclein is thought to be a central pathogenic event in the PD brain but there have not as yet been reports of retinal synucleinopathy. Retinal wholemounts were prepared from subjects with a primary clinicopathological diagnosis of PD (N = 9), dementia with Lewy bodies (DLB; N = 3), Alzheimer's disease (N = 3), progressive supranuclear palsy (N = 2) as well as elderly normal control subjects (N = 4). These were immunohistochemically stained with an antibody against α-synuclein phosphorylated at serine 129, which is a specific molecular marker of synucleinopathy. Phosphorylated α-synuclein-immunoreactive (p-syn IR) nerve fibers were present in 7/9 PD subjects and in 1/3 DLB subjects; these were sparsely distributed and superficially located near or at the inner retinal surface. The fibers were either long and straight or branching, often with multiple en-passant varicosities along their length. The straight fibers most often had an orientation that was radial with respect to the optic disk. Together, these features are suggestive of either retinopetal/centrifugal fibers or of ganglion cell axons. In one PD subject there were sparse p-syn IR neuronal cell bodies with dendritic morphology suggestive of G19 retinal ganglion cells or intrinsically photosensitive ganglion cells. There were no stained nerve fibers or other specific staining in any of the non-PD or non-DLB subjects. It is possible that at least some of the observed visual function impairments in PD subjects might be due to α-synucleinopathy.

Optic neuritis in pediatric population: A review in current tendencies of diagnosis and management

Optic neuritis is an inflammation of the optic nerve and may be related to different systemic conditions. The clinical presentation of this pathology usually includes sudden loss of visual acuity (VA) which may be unilateral or bilateral, visual field restriction, pain with eye movements, dyschromatopsia, a relative afferent pupillary defect and optic disk swelling. Optic neuritis in children has specific clinical features and a better prognosis than in adulthood. Although usually appears an underlying viral disease, the main concern for practitioners is the relationship of optic neuritis with multiple sclerosis. In addition to the classical techniques as magnetic resonance imaging (MRI), current tendencies of diagnosis for eye practitioners include new imaging devices as optical coherence tomography (OCT), useful to show a thinning of the retinal fibers layer (RFL) after the inflammatory episode. Regarding the management of these patients, short-term intravenous steroid dosages seem to be the best option to treat acute attacks characterized by a very poor bilateral VA.

Fitting of a New Design of Full Scleral Contact Lens in Advanced Keratoconus with Previous Implantation of Intracorneal Ring Segments

This case report reports the visual rehabilitation obtained with the fitting of a new design of full scleral contact lens (ICD 16.5 contact lens, Paragon Vision Sciences, distributed by Lenticon, Madrid, Spain) in a cornea with advanced keratoconus and previous implantation of intracorneal ring segment with a very limited effect. This eye had a refraction of –3.00 × 55° cylinder, providing a visual acuity of 0.5 LogMAR. The topographic pattern was very irregular with the presence of a significant central protrusion and a significant central corneal thinning. Some previous unsuccessful fittings have been performed with corneal and corneal-scleral lenses. A comfortable wearing was achieved with a fully scleral contact lens of 4600 μm of sagittal height, optical power of –11.25 D, and providing an apical clearance of 196 μm. A visual acuity of 0.0 LogMAR combined with a relevant aberrometric improvement was achieved with this contact lens. The patient was completely satisfied with the fitting. The result was maintained during 1 year after the fitting. Full scleral lenses are then able to provide comfortable wear and a significant increase in visual acuity combined with a significant improvement in the visual quality in eyes with advanced keratoconus.

Geochemistry, isotopic ratios and aldehyde concentrations of frost flowers, snow and ice near Barrow, Alaska

Frost flowers, intricate featherlike crystals that grow on refreezing sea ice leads, have been implicated in lower atmospheric chemical reactions. Few studies have presented chemical composition information for frost flowers over time and many of the chemical species commonly associated with Polar tropospheric reactions have never been reported for frost flowers. We undertook this study on the sea ice north of Barrow, Alaska to quantify the major ion, stable oxygen and hydrogen isotope, alkalinity, light absorbance by soluble species, organochlorine, and aldehyde composition of seawater, brine, and frost flowers. For many of these chemical species we present the first measurements from brine or frost flowers. Results show that major ion and alkalinity concentrations, stable isotope values, and major chromophore (NO3- and H2O2) concentrations are controlled by fractionation from seawater and brine. The presence of these chemical species in present and future sea ice scenarios is somewhat predictable. However, aldehydes, organochlorine compounds, light absorbing species, and mercury (part 2 of this research and Sherman et al. (2012, doi:10.1029/2011JD016186)) are deposited to frost flowers through less predictable processes that probably involve the atmosphere as a source. The present and future concentrations of these constituents in frost flowers may not be easily incorporated into future sea ice or lower atmospheric chemistry scenarios. Thinning of Arctic sea ice will likely present more open sea ice leads where young ice, brine, and frost flowers form. How these changing ice conditions will affect the interactions between ice, brine, frost flowers and the lower atmosphere is unknown.

Sedimentological and geophysical investigation of sediment cores from the Amundsen Sea

Ice loss from the marine-based, potentially unstable West Antarctic Ice Sheet (WAIS) contributes to current sea-level rise and may raise sea level by up to 3.3 to 5 meters in the future. Over the past few decades, glaciers draining the WAIS into the Amundsen Sea Embayment (ASE) have shown accelerated ice flow, rapid thinning and grounding-line retreat. However, the long-term context of this ice-sheet retreat is poorly constrained, limiting our ability to accurately predict future WAIS behaviour. Here we present a new chronology for WAIS retreat from the inner continental shelf of the eastern ASE based on radiocarbon dates from three marine sediment cores. The ages document a retreat of the grounding line to within ~93 km of its modern position before 11.7±0.7 kyr BP (thousand years before present). This early deglaciation is consistent with ages for grounding-line retreat from the western ASE. Our new data demonstrate that, other than in the Ross Sea, WAIS retreat in the ASE has not continued progressively since the Last Glacial Maximum. Furthermore, our results suggest that the grounding-line position in the ASE was predominantly stable throughout the Holocene, and that any episodes of fast retreat similar to that observed today must have been short-lived. Alternatively, today's rapid retreat was unprecedented during the Holocene. Therefore, the current ice loss must originate in recent changes in regional climate, ocean circulation or ice-sheet dynamics. Incorporation of these results into models is essential to produce robust predictions of future ice-sheet change and its contribution to sea-level rise.

(Table 1) Annual melt rates of the South Branch of Jacobshavn Isbrae between July 1984-1985

Photogrammetric reanalysis of 1985 aerial photos has revealed substantial submarine melting of the floating ice tongue of Jakobshavn Isbrae, west Greenland. The thickness of the floating tongue determined from hydrostatic equilibrium tapers from ~940 m near the grounding zone to ~600 m near the terminus. Feature tracking on orthophotos shows speeds on the July 1985 ice tongue to be nearly constant (~18.5 m/d), indicating negligible dynamic thinning. The thinning of the ice tongue is mostly due to submarine melting with average rates of 228 ± 49 m/yr (0.62 ± 0.13 m/d) between the summers of 1984 and 1985. The cause of the high melt rate is the circulation of warm seawater (thermal forcing of up to 4.2°C) beneath the tongue with convection driven by the substantial discharge of subglacial freshwater from the grounding zone. We believe that this buoyancy-driven convection is responsible for a deep channel incised into the sole of the floating tongue. A dramatic thinning, retreat, and speedup began in 1998 and continues today. The timing of the change is coincident with a 1.1°C warming of deep ocean waters entering the fjord after 1997. Assuming a linear relationship between thermal forcing and submarine melt rate, average melt rates should have increased by ~25% (~57 m/yr), sufficient to destabilize the ice tongue and initiate the ice thinning and the retreat that followed.

Bellingshausen Sea bathymetric compilation

The southern Bellingshausen Sea (SBS) is a rapidly-changing part of West Antarctica, where oceanic and atmospheric warming has led to the recent basal melting and break-up of the Wilkins ice shelf, the dynamic thinning of fringing glaciers, and sea-ice reduction. Accurate sea-floor morphology is vital for understanding the continued effects of each process upon changes within Antarctica's ice sheets. Here we present a new bathymetric grid for the SBS compiled from shipborne echo-sounder, spot-sounding and sub-ice measurements. The 1-km grid is the most detailed compilation for the SBS to-date, revealing large cross-shelf troughs, shallow banks, and deep inner-shelf basins that continue inland of coastal ice shelves. The troughs now serve as pathways which allow warm deep water to access the ice fronts in the SBS. Our dataset highlights areas still lacking bathymetric constraint, as well as regions for further investigation, including the likely routes of palaeo-ice streams. The new compilation is a major improvement upon previous grids and will be a key dataset for incorporating into simulations of ocean circulation, ice-sheet change and history. It will also serve forecasts of ice stability and future sea-level contributions from ice loss in West Antarctica, required for the next IPCC assessment report in 2013.

Volume changes of Jacobshavn Isbrae between 1985, 1997 and 2007

Following three decades of relative stability, Jakobshavn Isbrae, West Greenland, underwent dramatic thinning, retreat and speed-up starting in 1998. To assess the amount of ice loss, we analyzed 1985 aerial photos and derived a 40 m grid digital elevation model (DEM). We also obtained a 2007 40 m grid SPOT DEM covering the same region. Comparison of the two DEMs over an area of ~4000 km**2 revealed a total ice loss of 160 ± 4 km**3, with 107 ± 0.2 km**3 in grounded regions (0.27 mm eustatic sea-level rise) and 53 ± 4 km**3 from the disintegration of the floating tongue. Comparison of the DEMs with 1997 NASA Airborne Topographic Mapper data indicates that this ice loss essentially occurred after 1997, with +0.7 ± 5.6 km**3 between 1985 and 1997 and -160 ± 7 km**3 between 1997 and 2007. The latter is equivalent to an average specific mass balance of -3.7 ± 0.2 m/a over the study area. Previously reported thickening of the main glacier during the early 1990s was accompanied by similar-magnitude thinning outside the areas of fast flow, indicating that the land-based ice continued reacting to longer-term climate forcing.

Ground-based electromagnetic (EM) and drill-hole ice and snow thickness measurements during Polarstern cruise ARK-XVII/2 in 2001

Late-summer thickness distributions of large ice floes in the Transpolar Drift between Svalbard and the North Pole in 1991, 1996, 1998, and 2001 are compared. They have been derived from drilling and electromagnetic (EM) sounding. Results show a strong interannual variability, with significantly reduced thickness in 1998 and 2001. The mean thickness decreased by 22.5% from 3.11 m in 1991 to 2.41 m in 2001, and the modal thickness by 22% from 2.50 m in 1991 to 1.95 m in 2001. Since modal thickness represents the thickness of level ice, the observed thinning reflects changes in thermodynamic conditions. Together with additional data from the Laptev Sea obtained in 1993, 1995, and 1996, results are in surprising agreement with recently published thickness anomalies retrieved from satellite radar altimetry for Arctic regions south of 81.5°N. This points to a strong sensitivity of radar altimetry data to level ice thickness.

(Table 1) Sedimentary thicknesses, lithologies, ages, and interpreted paleowater depths for ODP Sites 135-840 and 135-841

The sedimentary succession drilled at Sites 840 and 841 on the Tonga forearc allows the sedimentary evolution of the active margin to be reconstructed since shortly after the initiation of subduction during the mid Eocene. Sedimentation has been dominated by submarine fan deposits, principally volcaniclastic turbidites and mass-flows derived from the volcanic arc. Volcaniclastic sedimentation occurred against a background of pelagic nannofossil sedimentation. A number of upward-fining cycles are recognized and are correlated to regional tectonic events, such as the rifting of the Lau Basin at 5.6 Ma. Episodes of sedimentation dating from 16.0 and 10.0 Ma also correlate well with major falls in eustatic sea level and may be at least partially caused by the resulting enhanced erosion of the arc edifice. The early stages of rifting of the Lau Basin are marked by the formation of a brief hiatus at Site 840 (Horizon A), probably a result of the uplift of the Tonga Platform. Controversy exists as to the degree and timing of the uplift of Site 840 before Lau Basin rifting, with estimates ranging from 2500 to 300 m. Structural information favors a lower value. Breakup of the Tonga Arc during rifting resulted in deposition of dacite-dominated, volcaniclastic mass flows, probably reflecting a maximum in arc volcanism at this time. A pelagic interval at Site 840 suggests that no volcanic arc was present adjacent to the Tonga Platform from 5.0 to 3.0 Ma. This represents the time between separation of the Lau Ridge from the Tonga Platform and the start of activity on the Tofua Arc at 3.0 Ma. The sedimentary successions at both sites provide a record of the arc volcanism despite the reworked nature of the deposits. Probe analyses of volcanic glass grains from Site 840 indicate a consistent low-K tholeiite chemistry from 7.0 Ma to the present, possibly reflecting sediment sourcing from a single volcanic center over long periods of time. Trace and rare-earth-element (REE) analyses of basaltic glass grains indicate that thinning of the arc lithosphere had begun by 7.0 Ma and was the principle cause of a progressive depletion of the high-field-strength (HFSE), REE, and large-ion-lithophile (LILE) elements within the arc magmas before rifting. Magmatic underplating of the Tofua Arc has reversed this trend since that time. Increasing fluid flux from the subducting slab since basin rifting has caused a progressive enrichment in LILEs. Subduction erosion of the underside of the forearc lithosphere has caused continuous subsidence and tilting toward the trench since 37.0 Ma. Enhanced subsidence occurred during rifting of the South Fiji and Lau basins. Collision of the Louisville Ridge with the trench has caused no change in the nature of the sedimentation, but it may have been responsible for up to 300 m of uplift at Site 840.

Quantification of retinal layer thickness changes in acute macular neuroretinopathy.

PURPOSE To quantitatively evaluate retinal layer thickness changes in acute macular neuroretinopathy (AMN). METHODS AMN areas were identified using near-infrared reflectance (NIR) images. Intraretinal layer segmentation using Heidelberg software was performed. The inbuilt ETDRS -grid was moved onto the AMN lesion and the mean retinal layer thicknesses of the central grid were recorded and compared with the corresponding area of the fellow eye at initial presentation and during follow-up. RESULTS Eleven patients were included (mean age 26±6 years). AMN lesions at baseline had a significantly thinner outer nuclear layer (ONL) (51±21 µm vs 73±17 µm, p=0.002). The other layers, including inner nuclear layer (37±8 µm vs 38±6 µm, p=0.9) and outer plexiform layer (OPL) (45±19 µm vs 33±16 µm, p=0.1) did not show significant differences between the study eyes and fellow eyes. Adjacent to NIR image lesions, areas of OPL thickening were identified (study eye: 50±14 µm vs fellow eye: 39±16 µm, p=0.005) with corresponding thinning of ONL (study eye: 52±16 µm vs fellow eye: 69±16 µm, p=0.002). CONCLUSIONS AMN presents with characteristic quantitative retinal changes and the extent of the lesion may be more extensive than initially presumed from NIR image lesions.

Long-range planning for developed sites in the Pacific Northwest : the context of hazard tree management /

"FPM-TP039-92."

Quantifying ice-shelf basal melt and ice-stream dynamics using high-resolution DEM and GPS time series

Thesis (Ph.D.)--University of Washington, 2016-06

Characterization of Organophosphate Pesticides in Urine and Home Environment Dust in an Agricultural Community

Thesis (Master's)--University of Washington, 2016-06