Female pipefish can detect the immune status of their mates

Given the ubiquity of the parasites and their important fitness consequences on mate and offspring condition, selection for the ability to distinguish healthy from parasitized potential mates is a key process to enhance Darwinian fitness. In this study, we experimentally evaluated how the immunological experience of two potential partners influences mate choice, using the sex-role-reversed pipefish Syngnathus typhle. We exposed S. typhle to immune challenges with heat-killed Vibrio bacteria and investigated whether the activation of the immune system determined mate preferences. Our results demonstrate that the immune status of the potential partners influenced female mate preference, such that females that were exposed to an immune challenge became choosy and favored unchallenged males. Males, however, did not show any preferences for female immune status. In this context, we discuss mate choice decisions and behavioral plasticity as a complex result of immune challenge, severity of infection, as well as trans-generational effects.

On the harmonic analysis of cup anemometer rotation speed: A principle to monitor performance and maintenance status of rotating meteorological sensors

The calibration results of one anemometer equipped with several rotors, varying their size, were analyzed. In each case, the 30-pulses pert turn output signal of the anemometer was studied using Fourier series decomposition and correlated with the anemometer factor (i.e., the anemometer transfer function). Also, a 3-cup analytical model was correlated to the data resulting from the wind tunnel measurements. Results indicate good correlation between the post-processed output signal and the working condition of the cup anemometer. This correlation was also reflected in the results from the proposed analytical model. With the present work the possibility of remotely checking cup anemometer status, indicating the presence of anomalies and, therefore, a decrease on the wind sensor reliability is revealed.

Present status of the Liquid Breeder Validation

One of the objectives of IFMIF (International Fusion Materials Irradiation Facility), as stated in its specifications, is the validation of breeder blanket concepts for DEMO design. The so-called Liquid Breeder Validation Module (LBVM) will be used in IFMIF to perform experiments under irradiation on functional materials related to liquid breeder concepts for future fusion reactors. This module, not considered in previous IFMIF design phases, is currently under design by CIEMAT in the framework of the IFMIF/EVEDA project. In this paper, the present status of the design of the LBVM is presented.

Pathogen-Induced Changes in the Antioxidant Status of the Apoplast in Barley Leaves

Leaves of two barley (Hordeum vulgare L.) isolines, Alg-R, which has the dominant Mla1 allele conferring hypersensitive race-specific resistance to avirulent races of Blumeria graminis, and Alg-S, which has the recessive mla1 allele for susceptibility to attack, were inoculated with B. graminis f. sp. hordei. Total leaf and apoplastic antioxidants were measured 24 h after inoculation when maximum numbers of attacked cells showed hypersensitive death in Alg-R. Cytoplasmic contamination of the apoplastic extracts, judged by the marker enzyme glucose-6-phosphate dehydrogenase, was very low (less than 2%) even in inoculated plants. Dehydroascorbate, glutathione, superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase were present in the apoplast. Inoculation had no effect on the total foliar ascorbate pool size or the redox state. The glutathione content of Alg-S leaves and apoplast decreased, whereas that of Alg-R leaves and apoplast increased after pathogen attack, but the redox state was unchanged in both cases. Large increases in foliar catalase activity were observed in Alg-S but not in Alg-R leaves. Pathogen-induced increases in the apoplastic antioxidant enzyme activities were observed. We conclude that sustained oxidation does not occur and that differential strategies of antioxidant response in Alg-S and Alg-R may contribute to pathogen sensitivity.

Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.

Precise mapping of DNA methylation patterns in CpG islands has become essential for understanding diverse biological processes such as the regulation of imprinted genes, X chromosome inactivation, and tumor suppressor gene silencing in human cancer. We describe a new method, MSP (methylation-specific PCR), which can rapidly assess the methylation status of virtually any group of CpG sites within a CpG island, independent of the use of methylation-sensitive restriction enzymes. This assay entails initial modification of DNA by sodium bisulfite, converting all unmethylated, but not methylated, cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA. MSP requires only small quantities of DNA, is sensitive to 0.1% methylated alleles of a given CpG island locus, and can be performed on DNA extracted from paraffin-embedded samples. MSP eliminates the false positive results inherent to previous PCR-based approaches which relied on differential restriction enzyme cleavage to distinguish methylated from unmethylated DNA. In this study, we demonstrate the use of MSP to identify promoter region hypermethylation changes associated with transcriptional inactivation in four important tumor suppressor genes (p16, p15, E-cadherin, and von Hippel-Lindau) in human cancer.

Georges de La Tour : the Status of Scholarship

Seventeenth-century French painter, Georges de La Tour, was a forgotten artist. His rediscovery in the nineteenth century set off a firestorm of research and a hunt to find more works by the artist. One problem after another arose as scholars attempted to define the artist by his works, his style, and the remnants of his personal history. There remains a volume of contradictory reports, authentication issues, and new scientific techniques which continue to influence study on the artist.

Mangrove, Crab and Shorebird Activity as Bioindicators to the Health Status of Mangrove Ecosystems along the Surinamese Coastline, South America

This study focused on the shorebird activity along the Surinamese coast in relation to the mangrove ecosystem health. The health of three estuarine mangrove areas was assessed using important bioindicators of the mangrove ecosystem: crabs, birds and mangroves. Mangrove vegetation was measured at Weg Naar Zee, Matapica canal delta and Coronie coast. Crab activity was measured by burrow and crab counts. Occurring shorebirds were also counted at these areas. The results show that mangrove regeneration and shorebird activity is significantly related to the health of the ecosystem. Weg Naar Zee was the most damaged and highest at risk. Matapica canal delta and the Coronie coast were the least damaged, with Coronie coast showing greatest health and biodiversity of the indicators.

On the status of conversion in present-day American English: controversial issues and corpus-based study

This paper examines both theoretical and practical issues related to conversion. A quite detailed characterization of the 5329 instances identified in a 300.000-word corpus of American English written in the late 90s is provided. The examples are grouped according to the type of conversion involved. Frequency and the internal structure of words are also considered and compared with the results obtained by earlier scholars. In spite of the limitations that a corpus study imposes, the conclusions obtained seem to suggest that any item, independent of its morphological structure, may undergo conversion and this may happen in any register. Moreover, conversion seems to be an important source of new items in American English nowadays.

The circumstellar environment and evolutionary status of the supergiant B[e] star Wd1-9

Context. Historically, supergiant (sg)B[e] stars have been difficult to include in theoretical schemes for the evolution of massive OB stars. Aims. The location of Wd1-9 within the coeval starburst cluster Westerlund 1 means that it may be placed into a proper evolutionary context and we therefore aim to utilise a comprehensive multiwavelength dataset to determine its physical properties and consequently its relation to other sgB[e] stars and the global population of massive evolved stars within Wd1. Methods. Multi-epoch R- and I-band VLT/UVES and VLT/FORS2 spectra are used to constrain the properties of the circumstellar gas, while an ISO-SWS spectrum covering 2.45−45μm is used to investigate the distribution, geometry and composition of the dust via a semi-analytic irradiated disk model. Radio emission enables a long term mass-loss history to be determined, while X-ray observations reveal the physical nature of high energy processes within the system. Results. Wd1-9 exhibits the rich optical emission line spectrum that is characteristic of sgB[e] stars. Likewise its mid-IR spectrum resembles those of the LMC sgB[e] stars R66 and 126, revealing the presence of equatorially concentrated silicate dust, with a mass of ~10−4M⊙. Extreme historical and ongoing mass loss (≳ 10−4M⊙yr−1) is inferred from the radio observations. The X-ray properties of Wd1-9 imply the presence of high temperature plasma within the system and are directly comparable to a number of confirmed short-period colliding wind binaries within Wd1. Conclusions. The most complete explanation for the observational properties of Wd1-9 is that it is a massive interacting binary currently undergoing, or recently exited from, rapid Roche-lobe overflow, supporting the hypothesis that binarity mediates the formation of (a subset of) sgB[e] stars. The mass loss rate of Wd1-9 is consistent with such an assertion, while viable progenitor and descendent systems are present within Wd1 and comparable sgB[e] binaries have been identified in the Galaxy. Moreover, the rarity of sgB[e] stars - only two examples are identified from a census of ~ 68 young massive Galactic clusters and associations containing ~ 600 post-Main Sequence stars - is explicable given the rapidity (~ 104yr) expected for this phase of massive binary evolution.

Current status of research on school refusal

School refusal behavior refers to the avoidance of a child attending school and/or persistent difficulty staying in the classroom throughout the school day. Based on a review of the scientific literature, the aim of this study is to describe the current state of research on school refusal, differentiating between the findings and progress made in Spain from those achieved in the international field. For this purpose, the significance of this phenomenon, in addition to associated risk factors and variables, will be reviewed in the child and youth population. In turn, the commonly used assessment methods and most recommended treatment proposals, mainly based on cognitive behavioral therapy, are discussed. The results reveal several gaps and subjects for debate in some areas of knowledge about school refusal behavior, with differences being found between Spanish and international studies. In conclusion, future studies and challenges in this field are required.