Plant elicitor peptides are conserved signals regulating direct and indirect antiherbivore defense

Insect-induced defenses occur in nearly all plants and are regulated by conserved signaling pathways. As the first described plant peptide signal, systemin regulates antiherbivore defenses in the Solanaceae, but in other plant families, peptides with analogous activity have remained elusive. In the current study, we demonstrate that a member of the maize (Zea mays) plant elicitor peptide (Pep) family, ZmPep3, regulates responses against herbivores. Consistent with being a signal, expression of the ZmPROPEP3 precursor gene is rapidly induced by Spodoptera exigua oral secretions. At concentrations starting at 5 pmol per leaf, ZmPep3 stimulates production of jasmonic acid, ethylene, and increased expression of genes encoding proteins associated with herbivory defense. These include proteinase inhibitors and biosynthetic enzymes for production of volatile terpenes and benzoxazinoids. In accordance with gene expression data, plants treated with ZmPep3 emit volatiles similar to those from plants subjected to herbivory. ZmPep3-treated plants also exhibit induced accumulation of the benzoxazinoid phytoalexin 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside. Direct and indirect defenses induced by ZmPep3 contribute to resistance against S. exigua through significant reduction of larval growth and attraction of Cotesia marginiventris parasitoids. ZmPep3 activity is specific to Poaceous species; however, peptides derived from PROPEP orthologs identified in Solanaceous and Fabaceous plants also induce herbivory-associated volatiles in their respective species. These studies demonstrate that Peps are conserved signals across diverse plant families regulating antiherbivore defenses and are likely to be the missing functional homologs of systemin outside of the Solanaceae.

Current developments in the use of stem cell for therapeutic neovascularisation: is the future therapy "cell-free"?

The plasticity and self-regenerative properties of stem cells have opened new avenues in regenerative medicine. Greater understanding of the biology of stem cells is followed by growing expectations of a rapid translation into alternative therapeutic options. Recent preclinical studies and clinical trials employing stem and progenitor cells from different sources have shown encouraging results. However, their underlying mechanisms are still poorly understood, the potential adverse effects and the discrepancy in efficacy remain to be further investigated. Their essential role in vessel regeneration has made endothelial progenitor cells (EPC) a suitable candidate for therapeutic applications aiming at tissue revascularisation. Recent evidence suggests that EPC contribute to neovascularisation not only by direct participation in tissue homeostasis but mainly via paracrine mechanisms. In future, novel therapeutic strategies could be based on EPC paracrine factors or synthetic factors, and replace cell transplantation.

Direct visualization of the outer membrane of mycobacteria and corynebacteria in their native state.

The cell envelope of mycobacteria, which include the causative agents of tuberculosis and leprosy, is crucial for their success as pathogens. Despite a continued strong emphasis on identifying the multiple chemical components of this envelope, it has proven difficult to combine its components into a comprehensive structural model, primarily because the available ultrastructural data rely on conventional electron microscopy embedding and sectioning, which are known to induce artifacts. The existence of an outer membrane bilayer has long been postulated but has never been directly observed by electron microscopy of ultrathin sections. Here we have used cryo-electron microscopy of vitreous sections (CEMOVIS) to perform a detailed ultrastructural analysis of three species belonging to the Corynebacterineae suborder, namely, Mycobacterium bovis BCG, Mycobacterium smegmatis, and Corynebacterium glutamicum, in their native state. We provide new information that accurately describes the different layers of the mycobacterial cell envelope and challenges current models of the organization of its components. We show a direct visualization of an outer membrane, analogous to that found in gram-negative bacteria, in the three bacterial species examined. Furthermore, we demonstrate that mycolic acids, the hallmark of mycobacteria and related genera, are essential for the formation of this outer membrane. In addition, a granular layer and a low-density zone typifying the periplasmic space of gram-positive bacteria are apparent in CEMOVIS images of mycobacteria and corynebacteria. Based on our observations, a model of the organization of the lipids in the outer membrane is proposed. The architecture we describe should serve as a reference for future studies to relate the structure of the mycobacterial cell envelope to its function.

How does country risk matter for foreign direct investment?

In this paper, we aim to identify the political and financial risk components that matter most for the activities of multinational corporations. Our paper is the first paper to comprehensively examine the impact of various components of not only political risk but also financial risk on inward FDI, from both long-run and short-run perspectives. Using a sample of 93 countries (including 60 developing countries) for the period 1985-2007, we find that among the political risk components, government stability, socioeconomic conditions, investment profile, internal conflict, external conflict, corruption, religious tensions, democratic accountability, and ethnic tensions have a close association with FDI flows. In particular, socioeconomic conditions, investment profile, and external conflict appear to be the most influential components of political risk in attracting foreign investment. Among the financial risk components, only exchange rate stability yields statistically significant positive coefficients when estimated only for developing countries. In contrast, current account as a percentage of exports of goods and services, foreign debt as a percentage of GDP, net international liquidity as the number of months of import cover, and current account as a percentage of GDP yield negative coefficients in some specifications. Thus, multinationals do not seem to consider seriously the financial risk of the host country.

Efficient Model-based 3D Tracking by Using Direct Image Registration

This thesis deals with the problem of efficiently tracking 3D objects in sequences of images. We tackle the efficient 3D tracking problem by using direct image registration. This problem is posed as an iterative optimization procedure that minimizes a brightness error norm. We review the most popular iterative methods for image registration in the literature, turning our attention to those algorithms that use efficient optimization techniques. Two forms of efficient registration algorithms are investigated. The first type comprises the additive registration algorithms: these algorithms incrementally compute the motion parameters by linearly approximating the brightness error function. We centre our attention on Hager and Belhumeur’s factorization-based algorithm for image registration. We propose a fundamental requirement that factorization-based algorithms must satisfy to guarantee good convergence, and introduce a systematic procedure that automatically computes the factorization. Finally, we also bring out two warp functions to register rigid and nonrigid 3D targets that satisfy the requirement. The second type comprises the compositional registration algorithms, where the brightness function error is written by using function composition. We study the current approaches to compositional image alignment, and we emphasize the importance of the Inverse Compositional method, which is known to be the most efficient image registration algorithm. We introduce a new algorithm, the Efficient Forward Compositional image registration: this algorithm avoids the necessity of inverting the warping function, and provides a new interpretation of the working mechanisms of the inverse compositional alignment. By using this information, we propose two fundamental requirements that guarantee the convergence of compositional image registration methods. Finally, we support our claims by using extensive experimental testing with synthetic and real-world data. We propose a distinction between image registration and tracking when using efficient algorithms. We show that, depending whether the fundamental requirements are hold, some efficient algorithms are eligible for image registration but not for tracking.

Influence of Current Pulse Shape on Directly Modulated Systems Performance in Metro Area Optical Networks

Due to the fact that a metro network market is very cost sensitive, direct modulated schemes appear attractive. In this paper a CWDM (Coarse Wavelength Division Multiplexing) system is studied in detail by means of an Optical Communication System Design Software; a detailed study of the modulated current shape (exponential, sine and gaussian) for 2.5 Gb/s CWDM Metropolitan Area Networks is performed to evaluate its tolerance to linear impairments such as signal-to-noise-ratio degradation and dispersion. Point-to-point links are investigated and optimum design parameters are obtained. Through extensive sets of simulation results, it is shown that some of these shape pulses are more tolerant to dispersion when compared with conventional gaussian shape pulses. In order to achieve a low Bit Error Rate (BER), different types of optical transmitters are considered including strongly adiabatic and transient chirp dominated Directly Modulated Lasers (DMLs). We have used fibers with different dispersion characteristics, showing that the system performance depends, strongly, on the chosen DML?fiber couple.

Impacto del cambio climático sobre los usos del agua en Europa

Esta Tesis realiza una contribución metodológica al estudio del impacto del cambio climático sobre los usos del agua, centrándose particularmente en la agricultura. Tomando en consideración su naturaleza distinta, la metodología aborda de forma integral los impactos sobre la agricultura de secano y la agricultura de regadío. Para ello incorpora diferentes modelos agrícolas y de agua que conjuntamente con las simulaciones de los escenarios climáticos permiten determinar indicadores de impacto basados en la productividad de los cultivos, para el caso de la agricultura de secano, e indicadores de impacto basados en la disponibilidad de agua para irrigación, para el caso de la agricultura de regadío. La metodología toma en consideración el efecto de la variabilidad climática en la agricultura, evaluando las necesidades de adaptación y gestión asociadas a los impactos medios y a la variabilidad en la productividad de los cultivos y el efecto de la variabilidad hidrológica en la disponibilidad de agua para regadío. Considerando la gran cantidad de información proporcionada por las salidas de las simulaciones de los escenarios climáticos y su complejidad para procesarla, se ha desarrollado una herramienta de cálculo automatizada que integra diferentes escenarios climáticos, métodos y modelos que permiten abordar el impacto del cambio climático sobre la agricultura, a escala de grandes extensiones. El procedimiento metodológico parte del análisis de los escenarios climáticos en situación actual (1961-1990) y futura (2071-2100) para determinar su fiabilidad y conocer qué dicen exactamente las proyecciones climáticas a cerca de los impactos esperados en las principales variables que intervienen en el ciclo hidrológico. El análisis hidrológico se desarrolla en los ámbitos territoriales de la planificación hidrológica en España, considerando la disponibilidad de información para validar los resultados en escenario de control. Se utilizan como datos observados las series de escorrentía en régimen natural estimadas el modelo hidrológico SIMPA que está calibrado en la totalidad del territorio español. Al trabajar a escala de grandes extensiones, la limitada disponibilidad de datos o la falta de modelos hidrológicos correctamente calibrados para obtener los valores de escorrentía, muchas veces dificulta el proceso de evaluación, por tanto, en este estudio se plantea una metodología que compara diferentes métodos de interpolación y alternativas para generar series anuales de escorrentía que minimicen el sesgo con respecto a los valores observados. Así, en base a la alternativa que genera los mejores resultados, se obtienen series mensuales corregidas a partir de las simulaciones de los modelos climáticos regionales (MCR). Se comparan cuatro métodos de interpolación para obtener los valores de las variables a escala de cuenca hidrográfica, haciendo énfasis en la capacidad de cada método para reproducir los valores observados. Las alternativas utilizadas consideran la utilización de la escorrentía directa simulada por los MCR y la escorrentía media anual calculada utilizando cinco fórmulas climatológicas basadas en el índice de aridez. Los resultados se comparan además con la escorrentía global de referencia proporcionada por la UNH/GRDC que en la actualidad es el “mejor estimador” de la escorrentía actual a gran escala. El impacto del cambio climático en la agricultura de secano se evalúa considerando el efecto combinado de los riesgos asociados a las anomalías dadas por los cambios en la media y la variabilidad de la productividad de los cultivos en las regiones agroclimáticas de Europa. Este procedimiento facilita la determinación de las necesidades de adaptación y la identificación de los impactos regionales que deben ser abordados con mayor urgencia en función de los riesgos y oportunidades identificadas. Para ello se utilizan funciones regionales de productividad que han sido desarrolladas y calibradas en estudios previos en el ámbito europeo. Para el caso de la agricultura de regadío, se utiliza la disponibilidad de agua para irrigación como un indicador del impacto bajo escenarios de cambio climático. Considerando que la mayoría de estudios se han centrado en evaluar la disponibilidad de agua en régimen natural, en este trabajo se incorpora el efecto de las infraestructuras hidráulicas al momento de calcular el recurso disponible bajo escenarios de cambio climático Este análisis se desarrolla en el ámbito español considerando la disponibilidad de información, tanto de las aportaciones como de los modelos de explotación de los sistemas hidráulicos. Para ello se utiliza el modelo de gestión de recursos hídricos WAAPA (Water Availability and Adaptation Policy Assessment) que permite calcular la máxima demanda que puede atenderse bajo determinados criterios de garantía. Se utiliza las series mensuales de escorrentía observadas y las series mensuales de escorrentía corregidas por la metodología previamente planteada con el objeto de evaluar la disponibilidad de agua en escenario de control. Se construyen proyecciones climáticas utilizando los cambios en los valores medios y la variabilidad de las aportaciones simuladas por los MCR y también utilizando una fórmula climatológica basada en el índice de aridez. Se evalúan las necesidades de gestión en términos de la satisfacción de las demandas de agua para irrigación a través de la comparación entre la disponibilidad de agua en situación actual y la disponibilidad de agua bajo escenarios de cambio climático. Finalmente, mediante el desarrollo de una herramienta de cálculo que facilita el manejo y automatización de una gran cantidad de información compleja obtenida de las simulaciones de los MCR se obtiene un proceso metodológico que evalúa de forma integral el impacto del cambio climático sobre la agricultura a escala de grandes extensiones, y a la vez permite determinar las necesidades de adaptación y gestión en función de las prioridades identificadas. ABSTRACT This thesis presents a methodological contribution for studying the impact of climate change on water use, focusing particularly on agriculture. Taking into account the different nature of the agriculture, this methodology addresses the impacts on rainfed and irrigated agriculture, integrating agricultural and water planning models with climate change simulations scenarios in order to determine impact indicators based on crop productivity and water availability for irrigation, respectively. The methodology incorporates the effect of climate variability on agriculture, assessing adaptation and management needs associated with mean impacts, variability in crop productivity and the effect of hydrologic variability on water availability for irrigation. Considering the vast amount of information provided by the outputs of the regional climate model (RCM) simulations and also its complexity for processing it, a tool has been developed to integrate different climate scenarios, methods and models to address the impact of climate change on agriculture at large scale. Firstly, a hydrological analysis of the climate change scenarios is performed under current (1961-1990) and future (2071-2100) situation in order to know exactly what the models projections say about the expected impact on the main variables involved in the hydrological cycle. Due to the availability of information for validating the results in current situation, the hydrological analysis is developed in the territorial areas of water planning in Spain, where the values of naturalized runoff have been estimated by the hydrological model SIMPA, which are used as observed data. By working in large-scale studies, the limited availability of data or lack of properly calibrated hydrological model makes difficult to obtain runoff time series. So as, a methodology is proposed to compare different interpolation methods and alternatives to generate annual times series that minimize the bias with respect to observed values. Thus, the best alternative is selected in order to obtain bias-corrected monthly time series from the RCM simulations. Four interpolation methods for downscaling runoff to the basin scale from different RCM are compared with emphasis on the ability of each method to reproduce the observed behavior of this variable. The alternatives consider the use of the direct runoff of the RCMs and the mean annual runoff calculated using five functional forms of the aridity index. The results are also compared with the global runoff reference provided by the UNH/GRDC dataset, as a contrast of the “best estimator” of current runoff on a large scale. Secondly, the impact of climate change on rainfed agriculture is assessed considering the combined effect of the risks associated with anomalies given by changes in the mean and variability of crop productivity in the agro-climatic regions of Europe. This procedure allows determining adaptation needs based on the regional impacts that must be addressed with greater urgency in light of the risks and opportunities identified. Statistical models of productivity response are used for this purpose which have been developed and calibrated in previous European study. Thirdly, the impact of climate change on irrigated agriculture is evaluated considering the water availability for irrigation as an indicator of the impact. Given that most studies have focused on assessing water availability in natural regime, the effect of regulation is incorporated in this approach. The analysis is developed in the Spanish territory considering the available information of the observed stream flows and the regulation system. The Water Availability and Adaptation Policy Assessment (WAAPA) model is used in this study, which allows obtaining the maximum demand that could be supplied under certain conditions (demand seasonal distribution, water supply system management, and reliability criteria) for different policy alternatives. The monthly bias corrected time series obtained by previous methodology are used in order to assess water availability in current situation. Climate change projections are constructed taking into account the variation in mean and coefficient of variation simulated by the RCM. The management needs are determined by the agricultural demands satisfaction through the comparison between water availability under current conditions and under climate change projections. Therefore, the methodology allows evaluating the impact of climate change on agriculture to large scale, using a tool that facilitates the process of a large amount of complex information provided by the RCM simulations, in order to determine the adaptation and management needs in accordance with the priorities of the indentified impacts.

Integration of socioeconomic and genetic aspects involved in the conservation of animal genetic resources = Integración de aspectos socioeconómicos y genéticos involucrados en la conservación de recursos genéticos animales

El sector ganadero está siendo gradualmente dominado por sistemas intensivos y especializados en los que los factores de producción están controlados y en los que los caracteres productivos son los criterios principales para la selección de especies y razas. Entretanto, muchos de los bienes y servicios que tradicionalmente suministraba el ganado, tales como los fertilizantes, la tracción animal o materias primas para la elaboración vestimenta y calzado están siendo reemplazados por productos industriales. Como consecuencia de ambos cambios, las razas seleccionadas intensivamente, las cuales están estrechamente ligadas a sistemas agrícolas de alta producción y altos insumos, han desplazado a muchas razas autóctonas, en las que la selección prácticamente ha cesado o es muy poco intensa. Actualmente existe una mayor conciencia social sobre la situación de las razas autóctonas y muchas funciones del ganado que previamente habían sido ignoradas están siendo reconocidas. Desde hace algunas décadas, se ha aceptado internacionalmente que las razas de ganado cumplen funciones económicas, socio-culturales, medioambientales y de seguridad alimentaria. Por ello, diferentes organismos internacionales han reconocido que la disminución de los recursos genéticos de animales domésticos (RGADs) es un problema grave y han recomendado su conservación. Aun así, la conservación de RGADs es un tema controvertido por la dificultad de valorar las funciones del ganado. Esta valoración es compleja debido que los RGADs tiene una doble naturaleza privada - pública. Como algunos economistas han subrayado, el ganado es un bien privado, sin embargo debido a algunas de sus funciones, también es un bien público. De esta forma, el aumento del conocimiento sobre valor de cada una de sus funciones facilitaría la toma de decisiones en relación a su conservación y desarrollo. Sin embargo, esta valoración es controvertida puesto que la importancia relativa de las funciones del ganado varía en función del momento, del lugar, de las especies y de las razas. El sector ganadero, debido a sus múltiples funciones, está influenciado por factores técnicos, medioambientales, sociales, culturales y políticos que están interrelacionados y que engloban a una enorme variedad de actores y procesos. Al igual que las funciones del ganado, los factores que afectan a su conservación y desarrollo están fuertemente condicionados por localización geográfica. Asimismo, estos factores pueden ser muy heterogéneos incluso dentro de una misma raza. Por otro lado, es razonable pensar que el ganadero es el actor principal de la conservación de razas locales. Actualmente, las razas locales están siendo Integration of socioeconomic and genetic aspects involved in the conservation of animal genetic resources 5 explotadas por ganaderos muy diversos bajo sistemas de producción también muy diferentes. Por todo ello, es de vital importancia comprender y evaluar el impacto que tienen las motivaciones, y el proceso de toma de decisiones de los ganaderos en la estructura genética de las razas. En esta tesis doctoral exploramos diferentes aspectos sociales, económicos y genéticos involucrados en la conservación de razas locales de ganado vacuno en Europa, como ejemplo de RGADs, esperando contribuir al entendimiento científico de este complejo tema. Nuestro objetivo es conseguir una visión global de los procesos subyacentes en la conservación y desarrollo de estas razas. Pretendemos ilustrar como se pueden utilizar métodos cuantitativos en el diseño y establecimiento de estrategias de conservación y desarrollo de RGADs objetivas y adecuadas. En primer lugar, exploramos el valor económico total (VET) del ganado analizando sus componentes públicos fuera de mercado usando como caso de estudio la raza vacuna Alistana-Sanabresa (AS). El VET de cualquier bien está formado por componentes de uso y de no-uso. Estos últimos incluyen el valor de opción, el valor de herencia y el valor de existencia. En el caso del ganado local, el valor de uso directo proviene de sus productos. Los valores de uso indirecto están relacionados con el papel que cumple las razas en el mantenimiento de los paisajes y cultura rural. El valor de opción se refiere a su futuro uso potencial y el valor de herencia al uso potencial de las generaciones venideras. Finalmente, el valor de existencia está relacionado con el bienestar que produce a la gente saber que existe un recurso específico. Nuestro objetivo fue determinar la importancia relativa que tienen los componentes fuera de mercado sobre el VET de la raza AS. Para ello evaluamos la voluntad de la gente a pagar por la conservación de la AS mediante experimentos de elección (EEs) a través de encuestas. Estos experimentos permiten valorar individualmente los distintos componentes del VET de cualquier bien. Los resultados los analizamos mediante de uso de modelos aleatorios logit. Encontramos que las funciones públicas de la raza AS tienen un valor significativo. Sus valores más importantes son el valor de uso indirecto como elemento cultural Zamorano y el valor de existencia (ambos representaron el 80% de VET). Además observamos que el valor que gente da a las funciones públicas de la razas de ganado dependen de sus características socioeconómicas. Los factores que condicionaron la voluntad a pagar para la conservación de la raza AS fueron el lugar de residencia (ciudad o pueblo), el haber visto animales de la raza o haber consumido sus productos y la actitud de los encuestados ante los conflictos entre el desarrollo económico y el medioambiente. Por otro lado, encontramos que no todo el mundo tiene una visión completa e integrada de todas las funciones públicas de la raza AS. Por este motivo, los programas o actividades de concienciación sobre su estado deberían hacer hincapié en este aspecto. La existencia de valores públicos de la raza AS implica que los ganaderos deberían recibir compensaciones económicas como pago por las funciones públicas que cumple su raza local. Las compensaciones asegurarían un tamaño de población que permitiría que la raza AS siga realizando estas funciones. Un mecanismo para ello podría ser el desarrollo del turismo rural relacionado con la raza. Esto aumentaría el valor de uso privado mientras que supondría un elemento añadido a las estrategias de conservación y desarrollo. No obstante, los ganaderos deben analizar cómo aprovechar los nichos de mercado existentes, así como mejorar la calidad de los productos de la raza prestando especial atención al etiquetado de los mismos. Una vez evaluada la importancia de las funciones públicas de las razas locales de ganado, analizamos la diversidad de factores técnicos, económicos y sociales de la producción de razas locales de ganado vacuno existente en Europa. Con este fin analizamos el caso de quince razas locales de ocho países en el contexto de un proyecto de colaboración internacional. Investigamos las diferencias entre los países para determinar los factores comunes clave que afectan a la viabilidad de las razas locales. Para ello entrevistamos mediante cuestionarios a un total de 355 ganaderos en las quince razas. Como indicador de viabilidad usamos los planes de los ganaderos de variación del tamaño de las ganaderías. Los cuestionarios incluían diferentes aspectos económicos, técnicos y sociales con potencial influencia en las dinámicas demográficas de las razas locales. Los datos recogidos los analizamos mediante distintas técnicas estadísticas multivariantes como el análisis discriminante y la regresión logística. Encontramos que los factores que afectan a la viabilidad de las razas locales en Europa son muy heterogéneos. Un resultado reseñable fue que los ganaderos de algunos países no consideran que la explotación de su raza tenga un alto valor social. Este hecho vuelve a poner de manifiesto la importancia de desarrollar programas Europeos de concienciación sobre la importancia de las funciones que cumplen las razas locales. Además los países analizados presentaron una alta variabilidad en cuanto a la importancia de los mercados locales en la distribución de los productos y en cuanto al porcentaje en propiedad del total de los pastos usados en las explotaciones. Este estudio reflejó la variabilidad de los sistemas y medios de producción (en el sentido socioeconómico, técnico y ecológico) que existe en Europa. Por ello hay que ser cautos en la implementación de las políticas comunes en los diferentes países. También encontramos que la variabilidad dentro de los países puede ser elevada debido a las diferencias entre razas, lo que implica que las políticas nacionales deber ser suficientemente flexibles para adaptarse a las peculiaridades de cada una de las razas. Por otro lado, encontramos una serie de factores comunes a la viabilidad de las razas en los distintos países; la edad de los ganaderos, la colaboración entre ellos y la apreciación social de las funciones culturales, medioambientales y sociales del ganado local. El envejecimiento de los ganaderos de razas locales no es solo un problema de falta de transferencia generacional, sino que también puede suponer una actitud más negativa hacia la inversión en las actividades ganaderas y en una menor capacidad de adaptación a los cambios del sector. La capacidad de adaptación de los ganaderos es un factor crucial en la viabilidad de las razas locales. Las estrategias y políticas de conservación comunes deben incluir las variables comunes a la viabilidad de las razas manteniendo flexibilidad suficiente para adaptarse a las especificidades nacionales. Estas estrategias y políticas deberían ir más allá de compensación económica a los ganaderos de razas locales por la menor productividad de sus razas. Las herramientas para la toma de decisiones ayudan a generar una visión amplia de la conservación y desarrollo de las razas locales. Estas herramientas abordan el diseño de estrategias de conservación y desarrollo de forma sistemática y estructurada. En la tercera parte de la tesis usamos una de estas herramientas, el análisis DAFO (Debilidades, Amenazas, Fortalezas y Oportunidades), con este propósito, reconociendo que la conservación de RGADs depende de los ganaderos. Desarrollamos un análisis DAFO cuantitativo y lo aplicamos a trece razas locales de ganado vacuno de seis países europeos en el contexto del proyecto de colaboración mencionado anteriormente. El método tiene cuatro pasos: 1) la definición del sistema; 2) la identificación y agrupación de los factores influyentes; 3) la cuantificación de la importancia de dichos factores y 4) la identificación y priorización de estrategias. Identificamos los factores utilizando multitud de agentes (multi-stakeholder appproach). Una vez determinados los factores se agruparon en una estructura de tres niveles. La importancia relativa de los cada uno de los factores para cada raza fue determinada por grupos de expertos en RGADs de los países integrados en el citado proyecto. Finalmente, desarrollamos un proceso de cuantificación para identificar y priorizar estrategias. La estructura de agrupación de factores permitió analizar el problema de la conservación desde el nivel general hasta el concreto. La unión de análisis específicos de cada una de las razas en un análisis DAFO común permitió evaluar la adecuación de las estrategias a cada caso concreto. Identificamos un total de 99 factores. El análisis reveló que mientras los factores menos importantes son muy consistentes entre razas, los factores y estrategias más relevantes son muy heterogéneos. La idoneidad de las estrategias fue mayor a medida que estas se hacían más generales. A pesar de dicha heterogeneidad, los factores influyentes y estrategias más importantes estaban ligados a aspectos positivos (fortalezas y oportunidades) lo que implica que el futuro de estas razas es prometedor. Los resultados de nuestro análisis también confirmaron la gran relevancia del valor cultural de estas razas. Las factores internos (fortalezas y debilidades) más importantes estaban relacionadas con los sistemas de producción y los ganaderos. Las oportunidades más relevantes estaban relacionadas con el desarrollo y marketing de nuevos productos mientras que las amenazas más importantes se encontraron a la hora de vender los productos actuales. Este resultado implica que sería fructífero trabajar en la motivación y colaboración entre ganaderos así como, en la mejora de sus capacidades. Concluimos que las políticas comunes europeas deberían centrarse en aspectos generales y ser los suficientemente flexibles para adaptarse a las singularidades de los países y las razas. Como ya se ha mencionado, los ganaderos juegan un papel esencial en la conservación y desarrollo de las razas autóctonas. Por ello es relevante entender que implicación puede tener la heterogeneidad de los mismos en la viabilidad de una raza. En la cuarta parte de la tesis hemos identificado tipos de ganaderos con el fin de entender cómo la relación entre la variabilidad de sus características socioeconómicas, los perfiles de las ganaderías y las dinámicas de las mismas. El análisis se ha realizado en un contexto sociológico, aplicando los conceptos de capital cultural y económico. Las tipologías se han determinado en función de factores socioeconómicos y culturales indicadores del capital cultural y capital económico de un individuo. Nuestro objetivo era estudiar si la tipología socioeconómica de los ganaderos afecta al perfil de su ganadería y a las decisiones que toman. Entrevistamos a 85 ganaderos de la raza Avileña-Negra Ibérica (ANI) y utilizamos los resultados de dichas entrevistas para ilustrar y testar el proceso. Definimos los tipos de ganaderos utilizando un análisis de clúster jerarquizado con un grupo de variables canónicas que se obtuvieron en función de cinco factores socioeconómicos: el nivel de educación del ganadero, el año en que empezó a ser ganadero de ANI, el porcentaje de los ingresos familiares que aporta la ganadería, el porcentaje de propiedad de la tierra de la explotación y la edad del ganadero. La tipología de los ganaderos de ANI resultó ser más compleja que en el pasado. Los resultados indicaron que los tipos de ganaderos variaban en muchos aspectos socioeconómicos y en los perfiles de sus Integration of socioeconomic and genetic aspects involved in the conservation of animal genetic resources 9 ganaderías. Los tipos de ganaderos determinados toman diferentes decisiones en relación a la modificación del tamaño de su ganadería y a sus objetivos de selección. Por otro lado, reaccionaron de forma diferente ante un hipotético escenario de reducción de las compensaciones económicas que les planteamos. En este estudio hemos visto que el capital cultural y el económico interactúan y hemos explicado como lo hacen en los distintos tipos de ganaderos. Por ejemplo, los ganaderos que poseían un mayor capital económico, capital cultural formal y capital cultural adquirido sobre la raza, eran los ganaderos cuyos animales tenían una mayor demanda por parte de otros ganaderos, lo cual podría responder a su mayor prestigio social dentro de la raza. Uno de los elementos claves para el futuro de la raza es si este prestigio responde a una superioridad genética de las animales. Esto ocurriría si los ganaderos utilizaran las herramientas que tienen a su disposición a la hora de seleccionar animales. Los tipos de ganaderos identificados mostraron también claras diferencias en sus formas de colaboración y en su reacción a una hipotética variación de las compensaciones económicas. Aunque algunos tipos de ganaderos mostraron un bajo nivel de dependencia a estas compensaciones, la mayoría se manifestaron altamente dependientes. Por ello cualquier cambio drástico en la política de ayudas puede comprometer el desarrollo de las razas autóctonas. La adaptación las políticas de compensaciones económicas a la heterogeneidad de los ganaderos podría aumentar la eficacia de las mismas por lo que sería interesante explorar posibilidades a este respecto. Concluimos destacando la necesidad de desarrollar políticas que tengan en cuenta la heterogeneidad de los ganaderos. Finalmente abordamos el estudio de la estructura genética de poblaciones ganaderas. Las decisiones de los ganaderos en relación a la selección de sementales y su número de descendientes configuran la estructura demográfica y genética de las razas. En la actualidad existe un interés renovado por estudiar las estructuras poblacionales debido a la influencia potencial de su estratificación sobre la predicción de valores genómicos y/o los análisis de asociación a genoma completo. Utilizamos dos métodos distintos, un algoritmo de clústeres basados en teoría de grafos (GCA) y un algoritmo de clustering bayesiano (STRUCTURE) para estudiar la estructura genética de la raza ANI. Prestamos especial atención al efecto de la presencia de parientes cercanos en la población y de la diferenciación genética entre subpoblaciones sobre el análisis de la estructura de la población. En primer lugar evaluamos el comportamiento de los dos algoritmos en poblaciones simuladas para posteriormente analizar los genotipos para 17 microsatélites de 13343 animales de 57 ganaderías distintas de raza ANI. La ANI es un ejemplo de raza con relaciones complejas. Por otro lado, utilizamos el archivo de pedigrí de la raza para estudiar el flujo de genes, calculando, entre otras cosas, la contribución de cada ganadería a la constitución genética de la raza. En el caso de las poblaciones simuladas, cuando el FST entre subpoblaciones fue suficientemente alto, ambos algoritmos, GCA y STRUCTURE, identificaron la misma estructura genética independientemente de que existieran o no relaciones familiares. Por el contrario, cuando el grado de diferenciación entre poblaciones fue bajo, el STRUCTURE identificó la estructura familiar mientras que GCA no permitió obtener ningún resultado concluyente. El GCA resultó ser un algoritmo más rápido y eficiente para de inferir la estructura genética en poblaciones con relaciones complejas. Este algoritmo también puede ser usado para reducir el número de clústeres a testar con el STRUTURE. En cuanto al análisis de la población de ANI, ambos algoritmos describieron la misma estructura, lo cual sugiere que los resultados son robustos. Se identificaron tres subpoblaciones diferenciadas que pudieran corresponderse con tres linajes distintos. Estos linajes estarían directamente relacionados con las ganaderías que han tenido una mayor contribución a la constitución genética de la raza. Por otro lado, hay un conjunto muy numeroso de individuos con una mezcla de orígenes. La información molecular describe una estructura estratificada de la población que se corresponde con la evolución demográfica de la raza. Es esencial analizar en mayor profundidad la composición de este último grupo de animales para determinar cómo afecta a la variabilidad genética de la población de ANI. SUMMARY Summary Livestock sector is gradually dominated by intensive and specialized systems where the production environment is controlled and the production traits are the main criteria for the selection of species and breeds. In the meantime, the traditional use of domestic animals for draught work, clothes and manure has been replaced by industrial products. As a consequence of both these changes, the intensively selected breeds closely linked with high-input highoutput production systems have displaced many native breeds where the selection has practically ceased or been very mild. People are now more aware of the state of endangerment among the native breeds and the previously ignored values of livestock are gaining recognition. For some decades now, the economic, socio-cultural, environmental and food security function of livestock breeds have been accepted worldwide and their loss has been recognized as a major problem. Therefore, the conservation of farm animal genetic resources (FAnGR) has been recommended. The conservation of FAnGR is controversial due to the complexity of the evaluation of its functions. This evaluation is difficult due to the nature of FAnGR both as private and public good. As some economists have highlighted, livestock animals are private goods, however, they are also public goods by their functions. Therefore, there is a need to increase the knowledge about the value of all livestock functions since to support the decision-making for the sustainable conservation and breeding of livestock. This is not straightforward since the relative importance of livestock functions depends on time, place, species and breed. Since livestock play a variety of roles, their production is driven by interrelated and everchanging economic, technical, environmental, social, cultural and political elements involving an enormous range of stakeholders. Not only FAnGR functions but also the importance of factors affecting the development and conservation of FAnGR can be very different across geographical areas. Furthermore, heterogeneity can be found even within breeds. Local breeds are nowadays raised by highly diverse farmers in equally diverse farms. It is quite reasonable to think that farmer is the major actor in the in situ conservation of livestock breeds. Thus, there is a need to understand the farmers’ motivations, decision making processes and the impact of their decisions on the genetic structure of breeds. In this PhD thesis we explore different social, economic and genetic aspects involved in the conservation of local cattle breeds, i.e. FAnGR, in Europe seeking to contribute to the scientific understanding of this complex issue. We aim to achieve a comprehensive view of the processes involved in the conservation and development of local cattle breeds and have made special efforts in discussing the implications of the research results in this respect. The final outcome of the thesis is to illustrate how quantitative methods can be exploited in designing and establishing sound strategies and programmes for the conservation and development of local livestock breeds. Firstly we explored the public non-market attributes of the total economic value (TEV) of livestock, using the Spanish Alistana-Sanabresa (AS) cattle breed as a case study. Total economic value of any good comprises both use and non-use components, where the latter include option, bequest and existence values. For livestock, the direct use values are mainly stemming from production outputs. Indirect use values relate to the role of livestock as a maintainer of rural culture and landscape. The option value is related to the potential use of livestock, the bequest values relate to the value associated with the inheritance of the resources to future generation and the existence values relate to the utility perceived by people from knowing that specific resources exist. We aimed to determine the relative importance of the non-market components of the TEV of the AS breed, the socio-economic variables that influence how people value the different components of TEV and to assess the implications of the Spanish national conservation strategy for the AS breed. To do so, we used a choice experiment (CE) approach and applied the technique to assess people’s willingness to pay (WTP) for the conservation of AS breed. The use of CE allows the valuation of the individual components of TEV for a given good. We analysed the choice data using a random parameter logit (RPL) model. AS breed was found to have a significant public good value. Its most important values were related to the indirect use value due to the maintenance of Zamorian culture and the existence value (both represent over 80% of its TEV). There were several socioeconomic variables influencing people’s valuation of the public service of the breed. In the case of AS breed, the place of living (city or rural area), having seen animals of the breed, having eaten breed products and the respondents’ attitude towards economic development – environment conflicts do influence people’s WTP for AS conservation. We also found that people do not have a complete picture of all the functions and roles that AS breed as AnGR. Therefore, the actions for increasing awareness of AS should go to that direction. The farmers will need incentives to exploit some of the public goods values and maintain the breed population size at socially desirable levels. One such mechanism could be related to the development of agritourism, which would enhance the private good value and provide an important addition to the conservation and utilisation strategy. However, the farmers need a serious evaluation on how to invest in niche product development or how to improve product quality and brand recognition. Using the understanding on the importance of the public function of local cattle we tried to depict the current diversity regarding technical, economic and social factors found in local cattle farming across Europe. To do so we focused in an international collaborative project on the case of fifteen local cattle breeds in eight European countries. We investigated the variation among the countries to detect the common key elements, which affect the viability of local breeds. We surveyed with interviews a total of 355 farms across the fifteen breeds. We used the planned herd size changes by the farmer as an indicator of breed viability. The questionnaire included several economic, technical and social aspects with potential influence on breeds’ demographic trends. We analysed the data using multivariate statistical techniques, such as discriminat analysis and logistic regression. The factors affecting a local breed’s viability were highly heterogeneous across Europe. In some countries, farmers did not recognise any high social value attached to keeping a local cattle breed. Hence there is a need to develop communication programmes across EU countries making people aware about the diversity and importance of values associated to raising local breeds. The countries were also very variable regarding the importance of local markets and the percentage of farm land owned by the farmers. Despite the country specificities, there were also common factors affecting the breed viability across Europe. The factors were from different grounds, from social, such as the age of the farmer and the social appreciation of their work, to technicalorganizational, such as the farmers’ attitude to collaborating with each other. The heterogeneity found reflects the variation in breeding systems and production environment (in the socioeconomic, technical and ecological sense) present in Europe. Therefore, caution should be taken in implementing common policies at the country level. Variability could also be rather high within countries due to breed specificities. Therefore, the national policies should be flexible to adapt to the specificities. The variables significantly associated with breed viability should be positively incorporated in the conservation strategies, and considered in developing common and/or national policies. The strategy preparation and policy planning should go beyond the provision of a general economic support to compensate farmers for the lower profitability of local breeds. Of particular interest is the observation that the opportunity for farmer collaboration and the appreciation by the society of the cultural, environmental and social role of local cattle farming were positively associated with the breed survival. In addition, farmer's high age is not only a problem of poor generation transfer but it is also a problem because it might lead to a lower attitude to investing in farming activities and to a lower ability to adapt to environment changes. The farmers’ adaptation capability may be a key point for the viability of local breeds. Decision making tools can help to get a comprehensive view on the conservation and development of local breeds. It allows us to use a systematic and structured approach for identifying and prioritizing conservation and development strategies. We used SWOT (Strengths, Weaknesses Opportunities and Threats) analysis for this purpose and recognized that many conservation and development projects rely on farmers. We developed a quantified SWOT method and applied it in the aforementioned collaborative research to a set of thirteen cattle breeds in six European countries. The method has four steps: definition of the system, identification and grouping of the driving factors, quantification of the importance of driving factors and identification and prioritization of the strategies. The factors were determined following a multi-stakeholder approach and grouped with a three level structure. FAnGR expert groups ranked the factors and a quantification process was implemented to identify and prioritize strategies. The structure of the SWOT analysis allowed analyzing the conservation problem from general down to specific perspectives. Joining breed specific analyses into a common SWOT analysis permitted comparison of breed cases across countries. We identified 99 driving factors across breeds. The across breed analysis revealed that irrelevant factors were consistent. There was high heterogeneity among the most relevant factors and strategies. The strategies increased eligibility as they lost specificity. Although the situation was very heterogeneous, the most promising factors and strategies were linked to the positive aspects (Strengths and Opportunities). Therefore, the future of the studied local breed is promising. The results of our analysis also confirmed the high relevance of the cultural value of the breeds. The most important internal factors (strengths and weaknesses) were related farmers and production systems. The most important opportunities were found in developing and marketing new products, while the most relevant threats were found in selling the current conventional products. In this regard, it should be fruitful to work on farmers’ motivation, collaboration, and capacity building. We conclude that European policies should focus on general aspects and be flexible enough to be adapted to the country and breed specificities. As mentioned, farmers have a key role in the conservation and development of a local cattle breed. Therefore, it is very relevant to understand the implications of farmer heterogeneity within a breed for its viability. In the fourth part of the thesis, we developed a general farmer typology to help analyzing the relations between farmer features and farm profiles, herd dynamics and farmers’ decision making. In the analysis we applied and used the sociological framework of economic and cultural capital and studied how the determined farmer types were linked to farm profiles and breeding decisions, among others. The typology was based on measurable socioeconomic factors indicating the economic and cultural capital of farmers. A group of 85 farmers raising the Spanish Avileña-Negra Ibérica (ANI) local cattle breed was used to illustrate and test the procedure. The farmer types were defined by a hierarchical cluster analysis with a set of canonical variables derived from the following five the socioeconomic factors: the formal educational level of the farmer, the year the farmer started keeping the ANI breed, the percentage of the total family income covered by the farm, the percentage of the total farm land owned by the farmer and the farmer’s age. The present ANI farmer types were much more complex than what they were in the past. We found that the farmer types differed in many socioeconomic aspects and in the farms profile. Furthermore, the types also differentiate farmers with respect to decisions about changing the farm size, breeding aims and stated reactions towards hypothetical subsidy variation. We have verified that economic and cultural capitals are not independent and further showed how they are interacting in the different farmer types. The farmers related to the types with high economic, institutionalized and embodied cultural capitals had a higher demand of breeding animals from others farmers of the breed, which may be related to the higher social prestige within the breed. One of the key implications of this finding for the future of the breed is whether or not the prestige of farmers is related to genetic superiority of their animals, what is to say, that it is related with a sound use of tools that farmers have available to make selection decisions. The farmer types differed in the form of collaboration and in the reactions to the hypothetical variation in subsidies. There were farmers with low dependency on subsidies, while most of them are highly dependent on subsidies. Therefore, any drastic change in the subsidy programme might have influence on the development of local breeds. The adaptation of these programme to the farmers’ heterogeneity might increase its efficacy, thus it would be interesting to explore ways of doing it. We conclude highlighting the need to have a variety of policies, which take into account the heterogeneity among the farmers. To finish we dealt with the genetic structure of livestock populations. Farmers’ decisions on the breeding animals and their progeny numbers shape the demographic and genetic structure of the breeds. Nowadays there is a renovated interest in studying the population structure since it can bias the prediction of genomic breeding values and genome wide association studies. We determined the genetic structure of ANI breed using two different methods, a graphical clustering algorithm (GCA) and a Bayesian clustering algorithm (STRUCTURE) were used. We paid particular attention to the influence that the presence of closely related individuals and the genetic differentiation of subpopulations may have on the inferences about the population structure. We first evaluated the performance of the algorithms in simulated populations. Then we inferred the genetic structure of the Spanish cattle breed ANI analysing a data set of 13343 animals (genotyped for 17 microsatellites) from 57 herds. ANI breed is an example of a population with complex relationships. We used the herdbook to study the gene flow, estimation among other things, the contribution of different herds to the genetic composition of the ANI breed. For the simulated scenarios, when FST among subpopulations was sufficiently high, both algorithms consistently inferred the correct structure regardless of the presence of related individuals. However, when the genetic differentiation among subpopulations was low, STRUCTURE identified the family based structure while GCA did not provide any consistent picture. The GCA was a fast and efficient method to infer genetic structure to determine the hidden core structure of a population with complex history and relationships. GCA could also be used to narrow down the number of clusters to be tested by STRUCTURE. Both, STRUCTURE and GCA describe a similar structure for the ANI breed suggesting that the results are robust. ANI population was found to have three genetically differentiated clusters that could correspond to three genetic lineages. These are directly related to the herds with a major contribution to the breed. In addition, ANI breed has also a large pool made of individuals with an admixture of origins. The genetic structure of ANI, assessed by molecular information, shows a stratification that corresponds to the demographic evolution of the breed. It will be of great importance to learn more about the composition of the pool and study how it is related to the existing genetic variability of the breed.

Selective ablation with UV lasers of a-Si:H thin film solar cells in direct scribing configuration

Monolithical series connection of silicon thin-film solar cells modules performed by laser scribing plays a very important role in the entire production of these devices. In the current laser process interconnection the two last steps are developed for a configuration of modules where the glass is essential as transparent substrate. In addition, the change of wavelength in the employed laser sources is sometimes enforced due to the nature of the different materials of the multilayer structure which make up the device. The aim of this work is to characterize the laser patterning involved in the monolithic interconnection process in a different configurations of processing than the usually performed with visible laser sources. To carry out this study, we use nanosecond and picosecond laser sources working at 355nm of wavelength in order to achieve the selective ablation of the material from the film side. To assess this selective removal of material has been used EDX (energy dispersive using X-ray) analysis

A silviculture-oriented spatio-temporal model for germination in Pinus pinea L. in the Spanish Northern Plateau based on a direct seeding experiment

Natural regeneration in Pinus pinea stands commonly fails throughout the Spanish Northern Plateau under current intensive regeneration treatments. As a result, extensive direct seeding is commonly conducted to guarantee regeneration occurrence. In a period of rationalization of the resources devoted to forest management, this kind of techniques may become unaffordable. Given that the climatic and stand factors driving germination remain unknown, tools are required to understand the process and temper the use of direct seeding. In this study, the spatio-temporal pattern of germination of P. pinea was modelled with those purposes. The resulting findings will allow us to (1) determine the main ecological variables involved in germination in the species and (2) infer adequate silvicultural alternatives. The modelling approach focuses on covariates which are readily available to forest managers. A two-step nonlinear mixed model was fitted to predict germination occurrence and abundance in P. pinea under varying climatic, environmental and stand conditions, based on a germination data set covering a 5-year period. The results obtained reveal that the process is primarily driven by climate variables. Favourable conditions for germination commonly occur in fall although the optimum window is often narrow and may not occur at all in some years. At spatial level, it would appear that germination is facilitated by high stand densities, suggesting that current felling intensity should be reduced. In accordance with other studies on P. pinea dispersal, it seems that denser stands during the regeneration period will reduce the present dependence on direct seeding.

Excitación y control de IGBTs de un inversor trifásico sinusoidal

El origen del proyecto se encuentra en la mejora de un inversor trifásico sinusoidal comercial sobre la base del estudio de las técnicas de excitación óptimas para los IGBTs que lo componen en su etapa de potencia. En las primeras fases de planteamiento del proyecto se propone una idea mucho más ambiciosa, la realización de un nuevo convertidor de emergencia, destinado al sector ferroviario, para dar servicio de climatización. Este convertidor está formado por la asociación en cascada de un bloque DC/DC elevador y un bloque inversor DC/AC trifásico controlado mediante PWM con modulación sinusoidal. Se pretendía así dar solución a las siguientes problemáticas detectadas en los convertidores comercializados hasta el momento: un bloque elevador excesivamente sobredimensionado, subsistemas de control independientes para los dos bloques que configuran el convertidor, adicionalmente, la tarjeta driver se rediseña con cada cambio de especificaciones por parte de un nuevo cliente y finalmente, las comunicaciones tanto de diagnosis como de mantenimiento necesitaban una importante actualización. Inicialmente, se ha realizado un estudio teórico de los bloques elevador e inversor para poder realizar el diseño y dimensionamiento de sus componentes tanto semiconductores como electromagnéticos. Una vez completada la parte de potencia, se estudia el control que se realiza mediante medidas directas y simulación tanto de la estrategia de control del elevador como del inversor. Así se obtiene una información completa de la funcionalidad de las tarjetas existentes. Se desea realizar el diseño de una única tarjeta controladora y una única tarjeta de drivers para ambos bloques. Por problemas ajenos, en el transcurso de este proyecto se cancela su realización comercial, con lo que se decide al menos crear la placa de control y poder gobernar un convertidor ya existente, sustituyendo la tarjeta de control del bloque elevador. Para poder fabricar la placa de control se divide en dos tarjetas que irán conectadas en modo sándwich. En una tarjeta está el microcontrolador y en otra está todo el interface necesario para operar con el sistema: entradas y salidas digitales, entradas y salidas analógicas, comunicación CAN, y un pequeño DC/DC comercial que proporciona alimentación al prototipo. Se realiza un pequeño programa funcional para poder manejar el convertidor, el cual con una tensión de 110V DC, proporciona a la salida una tensión de 380V AC. Como ya se ha expuesto, debido a la cancelación del proyecto industrial no se profundiza más en su mejora y se decide proponerlo para su evaluación en su fase actual. ABSTRACT. The beginning of the project is found in the improvement of a commercial sine wave three phase inverter which is based in a study about optimal excitation techniques to IGBTs which compose in the power stage. In the early phases of project it is proposed a much more ambitious idea, the fact of a new emergency converter, proposed for the rail sector to work in an air condition unit. This converter is formed by an association of a block cascaded DC/DC booster and a block DC/AC inverter three-phase controlled by a sine wave modulation PWM. The purposed was to give a solution to following problems detected in commercial converters nowadays: an excessively oversized block boost, independent control subsystems for two blocks that configure the converter. In addition, driver board is redesigned with each specifications change demand it a new customer, and finally, the communications, diagnostic and maintenance that needed a important upgrade. Initially, it has been performed a theoretical study of boost and the inverter blocks to be able to perform the component’s design and the size (semiconductor and electromagnetic fields). Once finished power study, it is analysed the control performed using direct measures and simulation of boost control strategy and inverter. With this it is obtained complete information about existing cards functionality. The project is looking for the design of just one controller card and one drivers´ card for both blocks. By unrelated problems, during the course of this project a commercial realization. So at least its decided to create control board to be able to existing converter, replacing boost block’s control board. To be able to manufacture control board it is divided in two cards connected in sandwiching mode. In a card is microcontroller and in another is all needed interface to operate with the system: digital inputs and outputs, analogical inputs and outputs, CAN communication, and a small DC / DC business that provide power supply to the prototype. It is performed a small functional program to handle the converter, which with an input voltage 110V DC provides an output voltage 380V AC. As already has been exposed, due to industrial project cancellation it is decided no to continue with all improvements and directly to evaluate it in the current phase.

Current collection by an active spherical electrode in an unmagnetized plasma

A theoretical model for the steady-state response of anodic contactors that emit a plasma current Ii and collect electrons from a collisionless, unmagnetized plasma is presented. The use of a (kinetic) monoenergetic population for the attracted species, well known in passive probe theory, gives both accuracy and tractability to the theory. The monoenergetic population is proved to behave like an isentropic fluid with radial plus centripetal motion, allowing direct comparisons with ad hoc fluid models. Also, a modification of the original monoenergetic equations permits analysis of contactors operating in orbit-limited conditions. Besides that, the theory predicts that, only for plasma emissions above certain threshold current a presheath/double layer/core structure for the potential is formed (the core mode), while for emissions below that threshold, a plasma contactor behaves exactly as a positive-ion emitter with a presheath/sheath structure (the no-core mode). Ion emitters are studied as a particular case. Emphasis is placed on obtaining dimensionless charts and approximate asymptotic laws of the current-voltage characteristic.

The Lagrangian description of aperiodic flows: a case study of the Kuroshio Current

This article reviews several recently developed Lagrangian tools and shows how their com- bined use succeeds in obtaining a detailed description of purely advective transport events in general aperiodic flows. In particular, because of the climate impact of ocean transport processes, we illustrate a 2D application on altimeter data sets over the area of the Kuroshio Current, although the proposed techniques are general and applicable to arbitrary time depen- dent aperiodic flows. The first challenge for describing transport in aperiodical time dependent flows is obtaining a representation of the phase portrait where the most relevant dynamical features may be identified. This representation is accomplished by using global Lagrangian descriptors that when applied for instance to the altimeter data sets retrieve over the ocean surface a phase portrait where the geometry of interconnected dynamical systems is visible. The phase portrait picture is essential because it evinces which transport routes are acting on the whole flow. Once these routes are roughly recognised it is possible to complete a detailed description by the direct computation of the finite time stable and unstable manifolds of special hyperbolic trajectories that act as organising centres of the flow.

Low Voltage Ride-through in DFIG Wind Generators by Controlling the Rotor Current without Crowbars

Among all the different types of electric wind generators, those that are based on doubly fed induction generators, or DFIG technology, are the most vulnerable to grid faults such as voltage sags. This paper proposes a new control strategy for this type of wind generator, that allows these devices to withstand the effects of a voltage sag while following the new requirements imposed by grid operators. This new control strategy makes the use of complementary devices such as crowbars unnecessary, as it greatly reduces the value of currents originated by the fault. This ensures less costly designs for the rotor systems as well as a more economic sizing of the necessary power electronics. The strategy described here uses an electric generator model based on space-phasor theory that provides a direct control over the position of the rotor magnetic flux. Controlling the rotor magnetic flux has a direct influence on the rest of the electrical variables enabling the machine to evolve to a desired work point during the transient imposed by the grid disturbance. Simulation studies have been carried out, as well as test bench trials, in order to prove the viability and functionality of the proposed control strategy.

Direct Vlasov simulations of electron-attracting cylindrical Langmuir probes in flowing plasmas

Current collection by positively polarized cylindrical Langmuir probes immersed in flowing plasmas is analyzed using a non-stationary direct Vlasov-Poisson code. A detailed description of plasma density spatial structure as a function of the probe-to-plasma relative velocity U is presented. Within the considered parametric domain, the well-known electron density maximum close to the probe is weakly affected by U. However, in the probe wake side, the electron density minimum becomes deeper as U increases and a rarified plasma region appears. Sheath radius is larger at the wake than at the front side. Electron and ion distribution functions show specific features that are the signature of probe motion. In particular, the ion distribution function at the probe front side exhibits a filament with positive radial velocity. It corresponds to a population of rammed ions that were reflected by the electric field close to the positively biased probe. Numerical simulations reveal that two populations of trapped electrons exist: one orbiting around the probe and the other with trajectories confined at the probe front side. The latter helps to neutralize the reflected ions, thus explaining a paradox in past probe theory.