620 resultados para Whitken, Marian


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La lectura histórica del territorio en relación con el sistema agroalimentario aporta elementos claves para reconstruir el sistema territorial, aprovechando la oportunidad que ofrece un renovado interés por la alimentación local y sostenible. El análisis histórico transdisciplinar incorpora variables espaciales, económicas, energéticas, urbanísticas, agronómicas y nutricionales y se centra en el tramo medio del valle del Duero (Castilla y León, España). Se trata de un territorio tradicionalmente agrícola, donde un producto de la tierra -el vino- es motor de innovación y ha transformado paisajes y estructuras. Aún así, se enfrenta a un desarrollo desigual e ilustra las contradicciones del mundo rural en un contexto alimentario globalizado. El análisis de la región desde 1900 permite constatar la relación entre la organización del territorio, el sistema agroalimentario, y cada una de las etapas nutricionales: a) la superación de la desnutrición está asociada a una agricultura familiar y al territorio de proximidad, que persiste en la zona hasta 1950; b) el modelo de consumo de masas y sobrealimentación, se basa en una agricultura industrializada y un territorio polarizado ligado al desarrollismo, que se extiende hasta 1985; c) finalmente, el modelo de consumo segmentado se apoya en una agricultura terciarizada y un territorio de enclaves en un contexto de globalización, que dura hasta nuestros días. En la última fase aparecen nuevos modelos alternativos de reconstrucción territorial con sistemas emergentes que reconectan campo y ciudad, consumo y producción desde sistemas de alimentación sostenible. Conviven dos tendencias: una hacia la jerarquización y el productivismo tecnificado y otra hacia la multifuncionalidad y la recampesinización que se reapropia de las innovaciones técnicas. La adaptación a las condiciones locales y aprovechar los recursos endógenos son elementos clave de sostenibilidad ambiental y social. Incorporar la alimentación en la planificación urbana y territorial desde una perspectiva agroecológica reduciría la insostenibilidad del sistema alimentario. Las propuestas de ordenación han de tener en cuenta la tipología de municipios, sus interrelaciones, las características agrológicas y productivas, la relación del muncipcon los núcleos de referencia y con las poblaciones que concentran las necesidades de alimentación. Se debe considerar asimismo la disponiblidad de infraestructuras, de equipamientos y de capital humano y relacional para fijar cadena de valor local. La ordenación urbanística cuenta ya con mecanismos como la clasificación del suelo, la regulación de usos y el diseño de redes de equipamientos que inciden sobre la autonomía de los sistema de alimentación locales y permiten fomentar la biodiversidad y las variedades locales. Son mecanismos insuficientemente aprovechados. Una adecuada utilización de los instrumentos de ordenación existentes, junto con el desarrollo de otros nuevos mejorarían de forma significativa la resiliencia de los sistemas agroalimentarios locales. ABSTRACT The historical review of the relationship between territory and agrifood system provides key lessons to help rebuild the territorial fabric, seizing the opportunity offered by a renewed interest in local and sustainable food. The historical transdisciplinary analysis spans spatial, economic, energy, agronomic and nutritional variables, focuses on the middle reaches of the Douro valley (Castilla y Leon, Spain). This a traditionally agricultural region, which has managed to turn a land product – the wine– into an engine of innovation which has transformed landscapes and structures. Even so, it faces the challenges of uneven development and illustrates the contradictions of the rural world in a globalized context. After the analysis of the evolution of the region since 1900, it can be concluded that the territory has been organized over time according to three models of food system that are in turn linked to different nutritional stages: a) the nutritional stage of overcoming malnutrition is related to family agriculture and a territory of proximity, which persists in the studied area until 1950; b) the model of mass consumption and overeating, was built on an industrialized agriculture and a polarized territory with unhindered development, which runs until 1985; c) and, finally, the model of consumer segmentation associated with terciarized agriculture and enclave territories in the context of globalization, which lasts until present time. During this last stage new alternative models of small-scale territorial reconstruction appear, linked to emerging systems that, based on sustainable food systems, reconnect city and countryside, consumption and production. Actually two trends coexist: one towards hierarchisation and tech-based productivism, and another one towards multifunctionality and peasantization that reappropriates technical innovations. The adaptation to local conditions taking advantage of local resources is a key element of environmental and social sustainability. Integrating food into urban and regional planning from an agroecological perspective would help reduce the current unsustainability of the food system. Planning proposals for municipalities need to consider their typology, agrological characteristics, productive capacity, links to other municipalities, proximity to reference nodes and population concentrations with food demands that need to be met. Availability of infrastructure, facilities, as well as human and relational capital to establish and reinforce local value chains is another aspect to consider in planning proposals. Spatial and urban planning are already equipped with mechanisms, such as land classification and the design of facilities’ networks, that affect the autonomy and stability of local food systems and can support biodiversity and adoption of local varieties. We are, however, missing opportunities. An adequate use of existing planning tools and the development of new ones could significantly improve the resilience of local agrifood systems.

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Euan Macrae was funded by a NERC Open CASE PhD award (NE/F013728/1) with Midland Valley Exploration Ltd. as the industry partner. We thank the 763 geoscientists for their participation, and in particular, the reference experts who gave their time freely to the project. Marian Scott (University of Glasgow, UK) is thanked for assisting with the statistical analysis. Four reviewers are thanked for their constructive comments which improved the manuscript.

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Acknowledgements The first author has been supported by a Georg Forster Research Fellowship granted by the Alexander von Humboldt Foundation, Germany

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The “peroxy” intermediate (P form) of bovine cytochrome c oxidase was prepared by reaction of the two-electron reduced mixed-valence CO complex with 18O2 after photolytic removal of CO. The water present in the reaction mixture was recovered and analyzed for 18O enrichment by mass spectrometry. It was found that approximately one oxygen atom (18O) per one equivalent of the P form was present in the bulk water. The data show that the oxygen–oxygen dioxygen bond is already broken in the P intermediate and that one oxygen atom can be readily released or exchanged with the oxygen of the solvent water.

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We describe a genome-wide characterization of mRNA transcript levels in yeast grown on the fatty acid oleate, determined using Serial Analysis of Gene Expression (SAGE). Comparison of this SAGE library with that reported for glucose grown cells revealed the dramatic adaptive response of yeast to a change in carbon source. A major fraction (>20%) of the 15,000 mRNA molecules in a yeast cell comprised differentially expressed transcripts, which were derived from only 2% of the total number of ∼6300 yeast genes. Most of the mRNAs that were differentially expressed code for enzymes or for other proteins participating in metabolism (e.g., metabolite transporters). In oleate-grown cells, this was exemplified by the huge increase of mRNAs encoding the peroxisomal β-oxidation enzymes required for degradation of fatty acids. The data provide evidence for the existence of redox shuttles across organellar membranes that involve peroxisomal, cytoplasmic, and mitochondrial enzymes. We also analyzed the mRNA profile of a mutant strain with deletions of the PIP2 and OAF1 genes, encoding transcription factors required for induction of genes encoding peroxisomal proteins. Induction of genes under the immediate control of these factors was abolished; other genes were up-regulated, indicating an adaptive response to the changed metabolism imposed by the genetic impairment. We describe a statistical method for analysis of data obtained by SAGE.

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The (X;1)(p11;q21) translocation is a recurrent chromosomal abnormality in a subset of human papillary renal cell carcinomas, and is sometimes the sole cytogenetic abnormality present. Via positional cloning, we were able to identify the genes involved. The translocation results in a fusion of the transcription factor TFE3 gene on the X chromosome to a novel gene, designated PRCC, on chromosome 1. Through this fusion, reciprocal translocation products are formed, which are both expressed in papillary renal cell carcinomas. PRCC is ubiquitously expressed in normal adult and fetal tissues and encodes a putative protein of 491 aa with a relatively high content of prolines. No relevant homologies with known sequences at either the DNA or the protein level were found.

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With the development of an insulin autoantibody (IAA) assay performed in 96-well filtration plates, we have evaluated prospectively the development of IAA in NOD mice (from 4 weeks of age) and children (from 7 to 10 months of age) at genetic risk for the development of type 1 diabetes. NOD mice had heterogeneous expression of IAA despite being inbred. IAA reached a peak between 8 and 16 weeks and then declined. IAA expression by NOD mice at 8 weeks of age was strongly associated with early development of diabetes, which occurred at 16–18 weeks of age (NOD mice IAA+ at 8 weeks: 83% (5/6) diabetic by 18 weeks versus 11% (1/9) of IAA negative at 8 weeks; P < .01). In man, IAA was frequently present as early as 9 months of age, the first sampling time. Of five children found to have persistent IAA before 1 year of age, four have progressed to diabetes (all before 3.5 years of age) and the fifth is currently less than age 2. Of the 929 children not expressing persistent IAA before age 1, only one has progressed to diabetes to date (age onset 3), and this child expressed IAA at his second visit (age 1.1). In new onset patients, the highest levels of IAA correlated with an earlier age of diabetes onset. Our data suggest that the program for developing diabetes of NOD mice and humans is relatively “fixed” early in life and, for NOD mice, a high risk of early development of diabetes is often determined by 8 weeks of age. With such early determination of high risk of progression to diabetes, immunologic therapies in humans may need to be tested in children before the development of IAA for maximal efficacy.

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DNA vaccines express antigens intracellularly and effectively induce cellular immune responses. Because only chimpanzees can be used to model human hepatitis C virus (HCV) infections, we developed a small-animal model using HLA-A2.1-transgenic mice to test induction of HLA-A2.1-restricted cytotoxic T lymphocytes (CTLs) and protection against recombinant vaccinia expressing HCV-core. A plasmid encoding the HCV-core antigen induced CD8+ CTLs specific for three conserved endogenously expressed core peptides presented by human HLA-A2.1. When challenged, DNA-immunized mice showed a substantial (5–12 log10) reduction in vaccinia virus titer compared with mock-immunized controls. This protection, lasting at least 14 mo, was shown to be mediated by CD8+ cells. Thus, a DNA vaccine expressing HCV-core is a potential candidate for a prophylactic vaccine for HLA-A2.1+ humans.

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The Snf1 protein kinase family has been conserved in eukaryotes. In the yeast Saccharomyces cerevisiae, Snf1 is essential for transcription of glucose-repressed genes in response to glucose starvation. The direct interaction between Snf1 and its activating subunit, Snf4, within the kinase complex is regulated by the glucose signal. Glucose inhibition of the Snf1-Snf4 interaction depends on protein phosphatase 1 and its targeting subunit, Reg1. Here we show that Reg1 interacts with the Snf1 catalytic domain in the two-hybrid system. This interaction increases in response to glucose limitation and requires the conserved threonine in the activation loop of the kinase, a putative phosphorylation site. The inhibitory effect of Reg1 appears to require the Snf1 regulatory domain because a reg1Δ mutation no longer relieves glucose repression of transcription when Snf1 function is provided by the isolated catalytic domain. Finally, we show that abolishing the Snf1 catalytic activity by mutation of the ATP-binding site causes elevated, constitutive interaction with Reg1, indicating that Snf1 negatively regulates its own interaction with Reg1. We propose a model in which protein phosphatase 1, targeted by Reg1, facilitates the conformational change of the kinase complex from its active state to the autoinhibited state.

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The pir gene of plasmid R6K encodes the protein, π, a replication and transcription factor. Two translational options for the pir gene give rise to two forms of π protein: a 35.0-kDa form (π35.0) and a shortened 30.5-kDa form (π30.5). Although both proteins bind to a series of 22-bp direct repeats essential for plasmid R6K replication, only π35.0 can bind to a site in the (A⋅T)-rich segment of its γ ori and activate the γ ori in vivo and in vitro. However, unlike π35.0, π30.5can inhibit in vivo and in vitro replication (activated by π35.0). We propose that the two forms of π might have distinct functions in replication. We show that although both forms of π produce dimers, the nature of these dimers is not identical. The N-terminal 37 amino acid residues appear to control the formation of the more stable π35.0 dimers, whereas another, apparently weaker interface holds together dimers of π30.5. We speculate that the leucine zipper-like motif, absent in π30.5, controls very specific functions of π protein.

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Building on the experiences of librarian representatives to curriculum committees in the colleges of dentistry, medicine, and nursing, the Health Science Center Libraries (HSCL) Strategic Plan recommended the formation of a Library Liaison Work Group to create a formal Library Liaison Program to serve the six Health Science Center (HSC) colleges and several affiliated centers and institutes. The work group's charge was to define the purpose and scope of the program, identify models of best practice, and recommend activities for liaisons. The work group gathered background information, performed an environmental scan, and developed a philosophy statement, a program of liaison activities focusing on seven |primary areas, and a forum for liaison communication. Hallmarks of the plan included intensive subject specialization (beyond collection development), extensive communication with users, and personal information services. Specialization was expected to promote competence, communication, confidence, comfort, and customization. Development of the program required close coordination with other strategic plan implementation teams, including teams for collection development, education, and marketing. This paper discusses the HSCL's planning process and the resulting Library Liaison Program. Although focusing on an academic health center, the planning process and liaison model may be applied to any library serving diverse, subject-specific user populations.

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The cytokinin group of plant hormones regulates aspects of plant growth and development, including the release of lateral buds from apical dominance and the delay of senescence. In this work the native promoter of a cytokinin synthase gene (ipt) was removed and replaced with a Cu-controllable promoter. Tobacco (Nicotiana tabacum L. cv tabacum) transformed with this Cu-inducible ipt gene (Cu-ipt) was morphologically identical to controls under noninductive conditions in almost all lines produced. However, three lines grew in an altered state, which is indicative of cytokinin overproduction and was confirmed by a full cytokinin analysis of one of these lines. The in vitro treatment of morphologically normal Cu-ipt transformants with Cu2+ resulted in delayed leaf senescence and an increase in cytokinin concentration in the one line analyzed. In vivo, inductive conditions resulted in a significant release of lateral buds from apical dominance. The morphological changes seen during these experiments may reflect the spatial aspect of control exerted by this gene expression system, namely expression from the root tissue only. These results confirmed that endogenous cytokinin concentrations in tobacco transformants can be temporally and spatially controlled by the induction of ipt gene expression through the Cu-controllable gene-expression system.