Fisiologia e metabolismo da Videira cv. Syrah no submédio do vale do São Francisco sob três estratégias de irrigação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

No contorno da serra: campesinato, cultura e turismo em Guaramiranga - CE

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identificação de marcas moleculares ligadas à ausência de sementes em videira

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Maturação in vitro de oócitos bovinos em meios suplementados com quercetina e seu efeito sobre o desenvolvimento embrionário

A quercetina é um flavonoide, amplamente encontrada em frutas, vegetais, grãos, flores, com elevada concentração no vinho tinto, e tem sido caracterizada funcionalmente pela atividade antioxidante. Para avaliação da maturação nuclear e do desenvolvimento embrionário bovino, os oócitos foram maturados por 22h na presença de quercetina (0,4, 2, 10 e 50µM), cisteamina (100µM) e na ausência dos antioxidantes. Os oócitos maturados foram corados com Hoechst para avaliação da maturação in vitro. Para avaliação do desenvolvimento embrionário, os oócitos foram fertilizados e cultivados in vitro, as taxas de desenvolvimento embrionário foram determinadas no sétimo dia de cultivo e o percentual de eclosão e o número de células dos embriões no oitavo dia. Os níveis de glutationa (GSH) dos oócitos foram mensurados por emissão de fluorescência com CMF₂HC. A porcentagem de maturação nuclear (±89%) não diferiu entre os grupos. O desenvolvimento embrionário variou entre os tratamentos, o percentual de blastocisto foi superior (P<0,05) nos grupos tratados com 0,4, 2, 10 e 50∝M de quercetina (56,9, 59,5, 53,6 e 49,6%, respectivamente) e com 100∝M de cisteamina (50,4%) em relação ao grupo controle (42,3%). Na comparação entre os dois antioxidantes, a quercetina (0,4 e 2µM) foi superior na produção de embriões (56,9 e 59,5%, respectivamente) em comparação com cisteamina (50,4%). As taxas de embriões eclodidos foram similares (P>0,05) entre os grupos (±63,0%). O número médio de células dos embriões também foi similar entre os grupos (±233). Os níveis intracelulares de GSH foram superiores nos oócitos maturados com cisteamina, mas similares entre os oócitos tratados com quercetina e o controle. A suplementação da maturação in vitro com antioxidantes melhora as taxas de blastocistos. A quercetina foi superior à cisteamina, que, por sua vez, foi superior ao controle. Mas os níveis de GSH foram superiores somente nos oócitos tratados com cisteamina.

Desenvolvimento de eletrodos modificados com poli ácido glutâmico e sua aplicação na análise de compostos antioxidantes e farmacêuticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação físico-química e sensorial de fermentado de acerola

Fruit processing for beverage production purposes is a way of adding value to the raw material and of raising the farmer s income by increasing the demand. Thus the objective of this work was the production of fermented West Indian cherry beverages and their physicochemical and sensory evaluation. The beverages were produced based on the Brazilian legislation for fermented fruit beverages and wine. The fermented beverages were produced from West Indian cherry pulp (pulper) and juice (press) and sweetened with sugar to obtain three types of beverage: dry, semidry and sweet. The beverages were submitted to the following physicochemical analyses: pH, alcohol, reducing sugars, total reducing sugars, total acidity, volatile acidity, fixed acidity, dry extract, reduced dry extract, alcohol to reduced dry extract ratio, free sulphur dioxide, total sulphur dioxide and turbidity. In the sensory analysis, the beverages were assessed using a 9-point hedonic scale, evaluating the attributes of appearance, odour, flavour and overall assessment. The chemical and sensory results were submitted to an analysis of variance and the means compared using Tukey s test (5%). Both the raw materials (pulp and juice) and the different sugar concentrations (dry, semidry and sweet) interfered in the sensory and physicochemical parameters of the fermented West Indian cherry beverages, the tasters showing preference for the sweetened beverages.

Própolis na produção de cachaça orgânica de qualidade

Pós-graduação em Microbiologia Agropecuária - FCAV

Produção de etanol utilizando mix de sorgo sacarino e cana-de-açúcar em processo de maturação

Pós-graduação em Microbiologia Agropecuária - FCAV

Extratos de moringa no tratamento do caldo de cana e reflexos sobre a fermentação alcoólica

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Produção de hidrolisado enzimático de farinha de batata-doce para fermentação etanólica

Recently there is a great quest of producing alcohol from starchy resources, replacing the sugar cane. The most common starchy sources are cassava, maize and sweet potatoes and a lot of research are been realized with excellent results. In this work it was evaluated the influence of the concentration of dry matter on the enzymatic hydrolysis process of starch from sweet potato for ethanol production. Through the sweet potato was produced a flour using a low-cost method and easy operation equipments. The sweet potato flour was characterized physical and chemically and from these results was prepared the treatments for enzymatic hydrolysis. The experimental design considered as independent variable the dry matter concentration of the sweet potato flour in 3 levels; 10, 15 and 20% in the formulation of suspensions. The other variables were keeping constant being: temperature in the 1° hydrolysis step of 90°C and time of 2 hours; temperature in the 2° saccharification step of 60°C and time of 17 hours. The hydrolysates obtained at the three assays were transferred to six liter enlerynmeyer and inoculated with a biologic catalyst, Saccharomyces, dehydrated yeasts of Saccharomyces cerevisiae CAT 1, at a rate of 5% in weight. The flasks were placed in a shaker type orbital with controlled temperature of 30°C during a time of 15 hours. The initial reducer sugars concentration and respective ethanol concentrations in wine were: 11.2% glucose and 2.16% ethanol in the suspension with 10% of dry matter; 13.5% glucose and 4.39% ethanol with 15% and 17.5% glucose and 6.03% ethanol in suspension with 20% of dry matter. ix The results showed that the higher percentage of dry matter carried out to higher sugar yield in hydrolyzed. It was possible observed that products quality improved with a higher concentration of dry matter

Comunidade de formigas (Hymenoptera: formicidae) em cultivo de videiras no município de São Roque - São Paulo

The cultivation of grapes is one of the most traditional cultures and the world's oldest one. In Brazil, cultivars occupy an area of approximately 90 thousand hectares, covering the south, southeast and northeast, in latitudes ranging from 30 º 56 '15''S up to 5 º 11' 15''S. The culture of the vine may be the target of the attack of some diseases and pests, and currently the main concern for growers is the subterranean mealybug, Eurhizococcus brasiliensis (Hemiptera: Margarodidae), known as pearl-ground. This arthropod infests the roots causing a progressive languish of the vine, even causes its death. In southern Brazil it is known that some species of ants can disperse pearl-ground for short distances, however, there is no information about that in the State of Sao Paulo. Thus, it is extremely important to perform studies with this focus on wine regions in this State. Therefore, this study aimed to identify the species of underground ant community in different grape varieties found in vineyards in the town of São Roque – SP, Brazil. With that purpose, samples were collected within one year. We used underground pitfalls spread across twelve points. In each of the points were placed two traps, one containing honey and the other containing sardine as attractive. The results revealed the presence of three morphospecies of ants in Niágara Rosada and four in other grape varieties, there was no significant difference in the species richness among the cultivars (Kruskal-Wallis - 0.33, p = 0.5). In both trials, the most frequent species was Brachymyrmex sp.4, suggesting that there is no dominance of distinct species of Niágara Rosada and the other grape varieties. There was no dissimilarity in species richness of the ones visiting honey or sardines (Kruskal-Wallis - 0.08, p = 0.7) and the analysis of variance indicated a great similarity in species richness between the rainy and dry seasons (Kruskal-Wallis - 0.09, p = 0.7)

Quantificação de carbamato de etila em vinhos

The ethyl carbamate is a compound present in most foods yeast-distillates; due to its carcinogenic potential, national legislation has established a limit of 0.150 µg / mL for spirits, so the values above the same, pose a threat to both public health as well for the economic sector by preventing the export of these products. The aim of this work is to provide the optimization of an analytical method employing the technique of gas chromatography equipped with flame ionization detector (FID) to determine the concentration levels of ethyl carbamate in some samples of wine. The use of ethyl acetate as solvent employed in the extraction, the ethyl carbamate present in wine samples proved to be suitable, where Recoveries were between 97.6 to 103.3% (m / m), with a standard deviation for between 0.56 to 3.50%. The concentrations of ethyl carbamate in particular wine samples vary between 3.22 µg / ml and 3.80 µg / mL, with a mean of 3.48 µg / mL. These valuesare all above the limit set by law. Thus, the results indicate the need for changes in the process of wine production, in order to control the levels of the substance

Cachaça tradicional e bidestilada: aceitação e perfis sensoriais

O mercado da cachaça, bebida tipicamente brasileira, vem crescendo muito no Brasil e no mundo. Porém, apesar do grande volume de produção, nosso país ainda exporta uma quantidade muito pequena do produto, cerca de 1%. Visando atingir o mercado internacional, e também atender às crescentes exigências do mercado interno, é ainda necessária uma melhor padronização dessa bebida, além da obtenção de um produto com boas características físico-químicas e sensoriais. O principal objetivo deste trabalho foi realizar estudos, visando avaliar o efeito do processo de bidestilação e do envelhecimento, na padronização e na qualidade sensorial, da cachaça comparando-se a aceitação de amostras de cachaça obtidas tradicionalmente, por bidestilação e também envelhecidas. Assim, foram produzidas oito amostras de cachaça, por destilação simples e utilizando-se o processo de bidestilação, em alambiques de cobre, a partir do mesmo vinho de cana, e então envelhecidas em tonéis de carvalho durante seis meses. As referidas amostras foram submetidas a testes de aceitação sensorial utilizando-se sessenta provadores e os resultados obtidos, mostraram que a bidestilação produz uma aguardente mais padronizada e ainda que sua aceitação, aumenta de forma mais acentuada durante o processo de envelhecimento

Compostos fenólicos de 10 variedades de uva

Phenolic compounds represent one of the main groups of secondary metabolites. Due to their chemical diversity, they have a variety of functions in plants, such as protection against ultraviolet radiation, herbivores and pathogens, and attraction of pollinators or dispersers of fruits and seeds. For human, the phenolic compounds are used like food colorings and flavors and, due to their pharmacological properties, mainly to antioxidant activity, they are associated with several health benefits, such as delay senility, prevention and therapy of cardiovascular diseases and of some cancers. The grapes are considered one of the main source of phenolic compounds and the fruit and its products are consumed in Brazil and worldwide. Considering the phenolic compounds diversity and their different distribution in the grape parts, this work had like aims the extraction, identification and quantification of the main classes of phenolic compounds of 10 grape varieties. The content of total phenols, flavanols and anthocyanins were determined, respectively, according to the Folin-Ciocalteu, DMACA and comparison of pH spectrophotometric methods. The content of total phenols varied from 142.75 + 1.86 to 483.39 + 5.90 mg.L-1 in the peel and from 86.50 + 0.54 to 146.32 + 9.97 mg.L-1 in the pulp. The amount of total flavanols varied from 3.68 + 0.03 to 6.92 + 0.26 mg.L-1 in the peel and from 0.90 + 0.00 to 1.36 + 0.00 mg.L-1 in the pulp. The content of total anthocyanins varied from 7.00 + 0.99 to 406.56 + 39.50 mg.L-1 in the peel and from 2.88 + 0.28 to 46.36 + 1.89 mg.L-1 in the pulp. The phenolic compounds concentration was higher in the peel than in the pulp. The total phenols and anthocyanins varied a lot while the total flavanols were more constant. The flavanols represent the smaller portion of phenolic compounds

Caracterização físico-química e sensorial de vinhos brancos da região noroeste de São Paulo

Brazilian wines are appreciated by consumers for having fruity odor and taste which are typical from Vitis labrusca cultivars. The rusticity of these cultivars, the winemaking and the physicochemical properties are some of the key factors to obtain a quality beverage. In this context, the aim of this article was to analyze the quality of three white wines from wineries located in northwest region of São Paulo state by determining physicochemical properties and sensory profile. Data were analyzed by statistical tests and by Principal Component Analysis (PCA). It was possible to observe statistical differences (P<0,05) in most physicochemical properties. Furthermore, the PCA showed that the attributes body and color significantly explained the variance of the first and second component, respectively, higlighting the Vitis vinifera wine. Thus the connection between the physicochemical and sensory properties of white wines was important for characterization of these beverages produced in the northwest of São Paulo.