Identifying yeats belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media

The purpose of this work was to compare and optimise different selective and differential media to aid in isolating spoilage yeasts belonging to the Brettanomyces/Dekkera genera. Growth media containing selective and differential factors were employed. These were inoculated with strains of yeast representing Spanish oenological microbiota. Lastly, some of these isolation media were successfully applied in 24 types of wine with a high ethylphenol content, all of which were from the Haro Oenological Station (La Rioja, Spain). p-coumaric acid was determined using High performance liquid chromatography-photodiode-array detection-electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS); 4-ethylphenol by using Solid phase micro extraction-gas chromatography-mass spectrometry (SPME-GC-MS); and the rest of the analysis was carried out using official OIV methodology. Actidione is the most effective selective factor for isolating Brettanomyces/Dekkera yeast genera. Other secondary selective factors (selective carbon sources, sorbic acid and ethanol as a microbicide agent) may be used successfully to eliminate potential false positivities; however, they slow growth and delay the time to obtain results.

Summa nobilitatis Hispanicæ, et immunitatis regiorum tributorum, causas, ius, ordinem, iudicium, & excusationem breuiter complectens : nunc postremò recognita, atque infinitis propè locis emendata, nouisque addictionibus aucta / authore Ioanne Arce ab Ot

Marca tip. en port. y en Z4r (Vindel, 413)

Summa nobilitatis Hispanicæ, et immunitatis regiorum tributorum, causas, ius, ordinem, iudicium, & excusationem breuiter complectens : nunc postremò recognita, atque infinitis propè locis emendata, nouisque addictionibus aucta / authore Ioanne Arce ab Otalora ... ; cui accessit rerum [et] verborum index copiosissimus

Marca tip. en port. y en Z4r (Vindel, 413)

Explicit summa Angelica de casib[us] [con]sci[enti]e / p[er] fratre[m] Angelu[m] d[e] clauasio co[m]pilata ; maxima cu[m] dilige[n]tia reuisa, [et] fideli studio emendata ; sicut ip[sum] op[us] p[er] se sat[is] attestabit[ur].

Huecos para iniciales

Opuscula / Sancti Thome ; quibus alias impressis nuper hec addidimus, v[idelic]et Summa[m] totius logice, Tractatum celeberrimum de vsuris nusq[uam] alias impressum.

Pie de imp. tomado del colofón en 2P7v

Secunda pars que dicitur summa summaru[m] siluestrina nuperrime magna cum diligentia recognita : adiectis etiam adnotatiunculis et numeris hactenus non impressis, huius quoq[ue] summe tabula in p[ar]tes duas diuisa est, sicut [et] ipsa su[m]ma, vt cuiq[ue

Pie de imp. tomado del colofón en 2Z5

Interpretatio Georgij Bruxcellensis in summulas magistri Petri hispani vna cu[m] magistri Thome Bricot questionibus de nouo in cuiusuis fine tractatus additis, Textu quoq[ue] suppositionu[m] de nouo readdito, ... summa cura ac diligentia de nouo emendata

Índice a tres col.

Summa summarum que siluestrina dicitur nuperrime magna cum diligentia recognita ... huius quoq[ue] sum[m]e tabula in p[ar]tes duas diuisa est, sicut [et] ipsa su[m]ma, vt cuiq[ue] p[ar]ti sua p[ar]s r[espo]ndeat ...

Pie de imp. tomado del colofón de la segunda parte en 2Z5

Summa Caietana de peccatis ; et Noui Testame[n]ti ientacula / reuerendissimi domini Thome de Vio cardinalis sa[n]cti Xisti ; [per ... Ioannem Danielis ... accuratis reuisa].

Editor literario tomado del colofón

Summa theologica [quarta pars]

Tit. tomado de Craviotto, I, 435

Summa uniuersae theologiae, siue quaestiones super quattuor libros sententiarum Pars tertia

Tít. tomado de Craviotto, I, 250