991 resultados para Solling, Gustav,
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Tübingen, Univ., Diss., 1852
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Tübingen, Univ., Diss., 1917
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mit e. Geleitwort von S. Ehrmann u. Proben aus d. Werken von Leopold Kompert [u.a.]. Buchschmuck von Gustav Böhm
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[Verf.: Wilhelm Johann Christian Casparson]
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Bern, Univ. Diss.
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bearb. von Gustav Adolf Schimmer. Hrsg. von der K. K. Statistischen Central-Commission
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hrsg. vom Vorstande der Gesellschaft zur Förderung der Wissenschaft des Judentums
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von H. Steinthal. Hrsg. von Gustav Karpeles
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Gustav Friedrich Oehler
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Membrane proteins carry out functions such as nutrient uptake, ATP synthesis or transmembrane signal transduction. An increasing number of reports indicate that cellular processes are underpinned by regulated interactions between these proteins. Consequently, functional studies of these networks at a molecular level require co-reconstitution of the interacting components. Here, we report a SNARE protein-based method for incorporation of multiple membrane proteins into artificial membrane vesicles of well-defined composition, and for delivery of large water-soluble substrates into these vesicles. The approach is used for in vitro reconstruction of a fully functional bacterial respiratory chain from purified components. Furthermore, the method is used for functional incorporation of the entire F1F0 ATP synthase complex into native bacterial membranes from which this component had been genetically removed. The novel methodology offers a tool to investigate complex interaction networks between membrane-bound proteins at a molecular level, which is expected to generate functional insights into key cellular functions.
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Membrane proteins carry out functions such as nutrient uptake, ATP synthesis or transmembrane signal transduction. An increasing number of reports indicate that cellular processes are underpinned by regulated interactions between these proteins. Consequently, functional studies of these networks at a molecular level require co-reconstitution of the interacting components. Here, we report a SNARE-protein based method for incorporation of multiple membrane proteins into membranes, and for delivery of large water-soluble substrates into closed membrane vesicles. The approach is used for in vitro reconstruction of a fully functional bacterial respiratory chain from purified components. Furthermore, the method is used for functional incorporation of the entire F1F0-ATP synthase complex into native bacterial membranes from which this component had been genetically removed. The novel methodology offers a tool to investigate complex interaction networks between membrane-bound proteins at a molecular level, which is expected to generate functional insights into key cellular functions.
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scripsit Gust. Frid. Oehler
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von Gustav Westphal
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von Georg Gustav Roskoff
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von Gustav Moritz Redslob