Algumas considerações sobre o princípio da culpa enquanto factor de autonomização do Direito das Contra-ordenações

Dissertação de mestrado em Direito Judiciário

Folate-targeted nanoparticles for rheumatoid arthritis therapy

Rheumatoid arthritis (RA) is the most common inflammatory rheumatic disease, affecting almost 1% of the world population. Although the cause of RA remains unknown, the complex interaction between immune mediators (cytokines and effector cells) is responsible for the joint damage that begins at the synovial membrane. Activated macrophages are critical in the pathogenesis of RA and have been shown to specifically express a receptor for the vitamin folic acid (FA), folate receptor (FR). This particular receptor allows internalization of FA-coupled cargo. In this review we will address the potential of nanoparticles as an effective drug delivery system for therapies that will directly target activated macrophages. Special attention will be given to stealth degree of the nanoparticles as a strategy to avoid clearance by macrophages of the mononuclear phagocytic system (MPS). This review summarizes the application of FA-target nanoparticles as drug delivery systems for RA and proposes prospective future directions.

Desarrollo de una técnica de detección rápida y precisa de preñez en cerdos. Segunda etapa: Purificación de factor precoz de preñez y desarrollo de un radioinmunoensayo

Una de las principales causas de pérdida reproductiva en condiciones normales, es la mortalidad embrionaria basal. Por lo tanto, estaría ampliamente justificado el diseño de experiencias que nos permitan encontrar las causas de las pérdidas y detectar la preñez tan precozmente, como fuere posible. Las causas de las pérdidas embrionarias en muchos casos se relacionan con fallas en las señales del embrión dirigidas a la madre con el fin de establecer inmunotolerancia, fenómeno imprescindible para la sobrevida del embrión porque modifica la respuesta inmune de modo favorable para éste. La inmunomodulación es ejercida por sustancias de naturaleza proteica detectadas durante la gestación. Entre estas se encuentra el Factor Precoz de Preñez (EPF). Durante la preñez, el EPF tiene dos fuentes de producción: una materna y otra embrionaria. El EPF materno, también llamado temprano, se produce durante los períodos pre y periimplantacional y persiste hasta el último tercio de la preñez. Hasta el presente, la única técnica desarrollada para la detección del EPF, es un ensayo in vitro propuesto por Morton que mide de manera indirecta la actividad del factor. Dicha técnica se basa en la capacidad que tiene el EPF de potenciar la acción de un suero antilinfocitario (SAL), para inhibir la formación de rosetas entre linfocitos T y eritrocitos heterólogos en presencia de complemento. Esta técnica es efectiva pero muy compleja para realizarse rutinariamente en el laboratorio. De todo lo expuesto surge claramente la necesidad del aislamiento y purificación del EPF, lo que permitiría desarrollar metodologías inmunológicas aplicables para el diagnóstico de rutina de detección de preñez en cerdos y un notable adelanto en el estudio de alguno de los factores que afectan los sucesos de concepción y el curso de preñez, momentos claves en la fisiología reproductiva. Objetivos Generales - Desarrollar una técnica de detección rápida y precisa del Factor Precoz de Preñez (EPF) en cerdos. Objetivos Específicos - Aislar, purificar y caracterizar al Factor Precoz de Preñez (EPF) a partir de suero y placenta de cerdas gestantes. - Obtener y valorar un antisuero policlonal anti-EPF en conejos a partir de la fracción purificada con mayor índice de actividad EPF.

Jacob - an activity regulated morphogenetic factor for synapto-dendritic cytoarchitecture

Magdeburg, Univ., Fak. für Naturwiss., Diss., 2008

Cross-talk of protein kinase B (PKB/Akt) with the transcription factor NFAT and the Src kinase Fyn in T lymphocytes

Magdeburg, Univ., Fak. für Naturwiss., Diss., 2010

Modulation of [My]-opioid receptor signal transduction and endocytosis by ADP-ribosylation factor proteins

Magdeburg, Univ., Fak. für Naturwiss., Diss., 2010

Konstruktion einer nicht-IGF-bindenden Insulin-like Growth Factor Binding Protein-2-Variante und Untersuchung ihrer Wirkung auf das Tumorzellwachstum

Magdeburg, Univ., Medizin. Fakultät, Diss., 2008

Regulation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-[kappa]B) by protein Kinase C theta (PKC-[theta]) and cylindromatosis (CYLD) in murine listeriosis and toxoplasmosis

Magdeburg, Univ., Fak. für Naturwiss., Diss., 2013

Width-weight relationship and condition factor of Ucides cordatus (Crustacea, Decapoda, Ucididae) at tropical mangroves of Northeast Brazil

The present contribution aims at evaluating the carapace width vs. humid weight relationship and the condition factor of Ucides cordatus (Linnaeus, 1763), in the mangrove forests of the Ariquindá and Mamucabas rivers, state of Pernambuco, Brazil. These two close areas present similar characteristics of vegetation and substrate, but exhibit different degrees of environmental conservation: the Ariquindá River is the preserved area, considered one of the last non-polluted of Pernambuco, while the Mamucabas River suffers impacts from damming, deforestation and deposition of waste. A total of 1,298 individuals of U. cordatus were collected. Males were larger and heavier than females, what is commonly observed in Brachyura. Ucides cordatus showed allometric negative growth (p < 0.05), which is probably related to the dilatation that this species develops in the lateral of the carapace, which stores six pairs of gills. The values of b were within the limit established for aquatic organisms. Despite of the condition factor being considered an important feature to confirm the reproductive period, since it varies with cyclic activities, in the present study it was not correlated to the abundance of ovigerous females. However, it was considered a good parameter to evaluate environmental impacts, being significantly lower at the impacted area.

Condition factor of Goniopsis cruentata (Crustacea, Brachyura, Grapsidae) from Mundaú/Manguaba estuarine complex, Alagoas, Brazil

The condition factor is a parameter which acts as a general indicator of the "well-being" of a species, and it can be obtained through the analysis of width vs. weight relationships. The present work aims to investigate size vs. weight relationship and the condition factor of the crab Goniopsis cruentata (Latreille, 1803). The study area was the Mundaú/Manguaba estuarine complex, Maceió, state of Alagoas, Northeast Brazil. Samplings were monthly accomplished from August 2007 to July 2008. A total of 626 individuals were analyzed, being 309 males and 317 females. Males were larger and heavier than females, what is expected in many brachyuran. The growth was positive allometric to both males (b = 3.42) and females (b = 3.30), not obeying the "cube law". The condition factor of female was higher than that of male crabs, probably due to the gonad weight of females. It also varied seasonally for both sexes, being higher in the autumn and winter in males, and in the autumn and spring in females, and related to the molt and period of spawning intensification.

Influência do propianato de testosterona e do "Diffusing factor" sôbre a cicatrização cutânea de lesões experimentais

The effect of testosterone propionate in different treatments was tested in adult male rats (250 g.) with mechanical skin experimental lesions. The whole period of cicatrization was investigated in normals, castrated and testosterone treated animals. We could not detect any alteration in the regeneration process in both treated and untreated rats (normals and castrated). Diffusing factor obtained from homologous testis, directly applied upon the lesions also do not change the healing period. Related to the course of the healing process, little evidence is presented by variance analysis that significative differences could be detected in the first periods, in both castrated and testosterone treated groups; however new well planed experiments should be carried to test this point.