Iowa Department of Transportation Major Items - Preliminary Quantity Report, May 20, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, May 20, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, June 17, 2014; With Addendums

Weekly letting report.

Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster Gαo-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036.

Regulator of G-protein signalling (RGS) proteins negatively regulate heterotrimeric G-protein signalling through their conserved RGS domains. RGS domains act as GTPase-activating proteins, accelerating the GTP hydrolysis rate of the activated form of Gα-subunits. Although omnipresent in eukaryotes, RGS proteins have not been adequately analysed in non-mammalian organisms. The Drosophila melanogaster Gαo-subunit and the RGS domain of its interacting partner CG5036 have been overproduced and purified; the crystallization of the complex of the two proteins using PEG 4000 as a crystallizing agent and preliminary X-ray crystallographic analysis are reported. Diffraction data were collected to 2.0 Å resolution using a synchrotron-radiation source.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, July 15, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, June 17, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, September 16, 2014; With Addendums

Weekly letting report.

Crystallization and preliminary X-ray analysis of monalysin, a novel β-pore-forming toxin from the entomopathogen Pseudomonas entomophila.

Monalysin was recently described as a novel pore-forming toxin (PFT) secreted by the Drosophila pathogen Pseudomonas entomophila. Recombinant monalysin is multimeric in solution, whereas PFTs are supposed to be monomeric until target membrane association. Monalysin crystals were obtained by the hanging-drop vapour-diffusion method using PEG 8000 as precipitant. Preliminary X-ray diffraction analysis revealed that monalysin crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 162.4, b = 146.2, c = 144.4 Å, β = 122.8°, and diffracted to 2.85 Å resolution using synchrotron radiation. Patterson self-rotation analysis and Matthews coefficient calculation indicate that the asymmetric unit contains nine copies of monalysin. Heavy-atom derivative data were collected and a Ta6Br14 cluster derivative data set confirmed the presence of ninefold noncrystallographic symmetry.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, August 19, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, August 19, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, October 21, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, September 16, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, November 18, 2014; With Addendums

Weekly letting report.