TRM1, a YY1-like suppressor of rbcS-m3 expression in maize mesophyll cells

The genes rbcS and rbcL encode, respectively, the small and large subunits of the photosynthetic carbon dioxide fixation enzyme ribulose bisphosphate carboxylase/oxygenase. There is a single rbcL gene in each chloroplast chromosome; a family of rbcS genes is located in the nuclear genome. These two genes are not expressed in mesophyll cells but are in adjacent bundle-sheath cells of leaves of the C4 plant Zea mays. Two regions of the maize gene rbcS-m3 are required for suppressing expression in mesophyll cells. One region is just beyond the translation termination site in the 3′ region, and the other is several hundred base pairs upstream of the transcription start site. A binding site for a protein with limited homology to the viral, yeast, and mammalian transcription repressor-activator YY1 (Yin-Yang I), has now been identified in the 3′ region. A maize gene for a protein with zinc fingers homologous to those of YY1 has been isolated, characterized, and expressed in Escherichia coli. The gene is designated trm1 (transcription repressor-maize 1). The protein TRM1 binds to the YY1-like site and, in addition, TRM1 binds to two sequence regions in the 5′ region of the gene that have no homology to the YY1 site. Mutagenesis or deletion of any of these three sequences eliminates repression of rbcS-m3 reporter genes in mesophyll cells.

The glycine binding site of the N-methyl-D-aspartate receptor subunit NR1: identification of novel determinants of co-agonist potentiation in the extracellular M3-M4 loop region.

The N-methyl-D-aspartate (NMDA) subtype of ionotropic glutamate receptors is a heterooligomeric membrane protein composed of homologous subunits. Here, the contribution of the M3-M4 loop of the NR1 subunit to the binding of glutamate and the co-agonist glycine was investigated by site-directed mutagenesis. Substitution of the phenylalanine residues at positions 735 or 736 of the M3-M4 loop produced a 15- to 30-fold reduction in apparent glycine affinity without affecting the binding of glutamate and the competitive glycine antagonist 7-chlorokynurenic acid; mutation of both residues caused a >100-fold decrease in glycine affinity. These residues are found in a C-terminal region of the M3-M4 loop that shows significant sequence similarity to bacterial amino acid-binding proteins. Epitope tagging revealed both the N-terminus and the M3-M4 loop to be exposed extracellularly, whereas a C-terminal epitope was localized intracellularly. These results indicate that the M3-M4 loop is part of the ligand-binding pocket of the NR1 subunit and provide the basis for a refined model of the glycine-binding site of the NMDA receptor.

A single residue in the M2-M3 loop is a major determinant of coupling between binding and gating in neuronal nicotinic receptors.

Binding of agonists to nicotinic acetylcholine receptors generates a sequence of changes that activate a cation-selective conductance. By measuring electrophysiological responses in chimeric alpha7/alpha3 receptors expressed in Xenopus oocytes, we have showed the involvement of the M2-M3 loop in coupling agonist binding to the channel gate. An aspartate residue therein, Asp-266 in the alpha7 subunit, was identified by site-directed mutagenesis as crucial, since mutants at this position exhibited very poor functional responses to three different nicotinic agonists. We have extended this investigation to another neuronal nicotinic receptor (alpha3/beta4), and found that a homologous residue in the beta4 subunit, Asp-268, played a similar role in coupling. These findings are consistent with a hypothesis that the aspartate residue in the M2-M3 loop, which is conserved in all homomer-forming alpha-type subunits and all neuronal beta-type subunits that combine to form functional receptors, is a major determinant of information transmission from binding site to channel gate in all neuronal nicotinic receptors.

Red/far-red and blue light-responsive regions of maize rbcS-m3 are active in bundle sheath and mesophyll cells, respectively.

Leaves of the C4 plant maize have two major types of photosynthetic cells: a ring of five large bundle sheath cells (BSC) surrounds each vascular bundle and smaller mesophyll cells (MC) lie between the cylinders of bundle sheath cells. The enzyme ribulose bisphosphate carboxylase/oxygenase is encoded by nuclear rbcS and chloroplast rbcL genes. It is not present in MC but is abundant in adjacent BSC of green leaves. As reported previously, the separate regions of rbcS-m3, which are required for stimulating transcription of the gene in BSC and for suppressing expression of reporter genes in MC, were identified by an in situ expression assay; expression was not suppressed in MC until after leaves of dark-grown seedlings had been illuminated for 24 h. Now we have found that transient expression of rbcS-m3 reporter genes is stimulated in BSC via a red/far-red reversible phytochrome photoperception and signal transduction system but that blue light is required for suppressing rbcS-m3 reporter gene expression in MC. Blue light is also required for the suppression system to develop in MC. Thus, the maize gene rbcS-m3 contains certain sequences that are responsive to a phytochrome photoperception and signal transduction system and other regions that respond to a UVA/blue light photoperception and signal transduction system. Various models of "coaction" of plant photoreceptors have been advanced; these observations show the basis for one type of coaction.

Comércio, bens de prestígio e insígnias de poder: as agências centro-ocidentais africanas nos relatos de viagem de Henrique de Carvalho em sua expedição à Lunda (1884-1888)

Ao longo da segunda metade do século XIX, a região da África Centro-Ocidental foi palco do processo de intensificação de expedições europeias rumo ao interior do continente que conjugavam interesses econômicos, políticos e científicos. Esta pesquisa busca analisar o papel de relevo ocupado pela cultura material na agenda científica da expedição portuguesa à Lunda chefiada pelo militar português Henrique de Carvalho entre 1884 e 1888. Pretendemos também avaliar as potencialidades que o estudo sobre os objetos apresentam enquanto fontes para a compreensão mais ampla acerca das agências históricas africanas. Para tanto, selecionamos as obras Descripção da Viagem à Mussumba do Muatiânvua (1890-1894) e Ethnographia e História Tradicional dos Povos da Lunda (1890) e, de maneira complementar, o Album da Expedição ao Muatianvua (1887) e o catálogo Collecção Henrique de Carvalho (Expedição à Lunda), editado pela Sociedade de Geografia de Lisboa (1896). Assim, pela ótica da história social, pretendemos investigar como as exigências e predileções centro-africanas por determinados artigos moldaram as relações comerciais travadas nesse período, abordando os processos de incorporação e ressignificação de objetos particularmente, bens de prestígio e insígnias de poder - interpretados como expressões de identidades, códigos sociais e hierarquias políticas no âmbito dessas sociedades e de suas relações com os europeus.

World Atmospheric Temperature Isotherms (January), 1884 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Karte der Januar-Isotherme. It is part of a two map set: [Karten der Isothermen, von Alexander Supan]. It was published by Ed. Holzel in 1884. Scale [ca. 1:110,000,000]. Map in German. The image inside the map neatline is georeferenced to the surface of the earth and fit to a non-standard 'Mercator' projection with the central meridian at 17.666 degrees west. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, selected cities, shoreline features, and more. Isotherms are shown at 2 degree intervals for January. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection and the Harvard University Library as part of the Open Collections Program at Harvard University project: Organizing Our World: Sponsored Exploration and Scientific Discovery in the Modern Age. Maps selected for the project correspond to various expeditions and represent a range of regions, originators, ground condition dates, scales, and purposes.

World Atmospheric Temperature Isotherms (July), 1884 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Karte der Juli-Isothermen. It is part of a two map set: [Karten der Isothermen, von Alexander Supan]. It was published by Ed. Holzel in 1884. Scale [ca. 1:110,000,000]. Map in German. The image inside the map neatline is georeferenced to the surface of the earth and fit to a non-standard 'Mercator' projection with the central meridian at 17.666 degrees west. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, selected cities, shoreline features, and more. Isotherms are shown at 2 degree intervals for July. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection and the Harvard University Library as part of the Open Collections Program at Harvard University project: Organizing Our World: Sponsored Exploration and Scientific Discovery in the Modern Age. Maps selected for the project correspond to various expeditions and represent a range of regions, originators, ground condition dates, scales, and purposes.

Providence, Rhode Island, -- Sewers, 1884 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Topographical map of the city of Providence showing proposed sewerage system together with sewers already constructed, compiled in the City Engineers Office. It was published Oct. 24, 1884 by the City Engineers Office, Sewer Dept. Scale [ca. 1:20,000]. Covers the city of Providence, Rhode Island and portions of the surrounding towns/cities. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Rhode Island State Plane Coordinate System (Feet) (FIPS 3800). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as roads, railroads, topography, drainage, selected buildings, constructed and proposed sewer lines, town boundaries, city districts, and more. Relief shown by contours. This layer is part of a selection of digitally scanned and georeferenced historic maps of New England from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of regions, originators, ground condition dates, scales, and map purposes.

Washington, D.C., 1884 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Topographical map of the District of Columbia and a portion of Virginia, compiled under the direction of Major G.J. Lydecker, Corps of Engineers, Engineer Commissioner D.C., by Captain F.V. Greene, Corps of Engineers ; drawn by W.T.O. Bruff. It was published in 1884. Scale [1:15,840]. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Maryland State Plane Coordinate System Meters NAD83 (Fipszone 1900). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads, drainage, selected public buildings, selected private residences and names of landowners, built-up areas, parks, and more. Relief shown by contours. Depths shown by soundings. Includes source materials note. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

New Orleans, Louisiana, 1884 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Map of the city of New Orleans showing location of exposition grounds and all approaches thereto by land & water, [by] the World's Industrial and Cotton Centennial Exposition, New Orleans, La., U.S.A., Department of Installation. It was published by The Exposition ca. 1884. Scale [ca. 1:2,000]. Covers also adjacent portions of Jefferson and St. Bernard Parishes. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Louisiana State Plane Coordinate System, South NAD83 (in Feet) (Fipszone 1702). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as exposition grounds, railroads, roads, canals, levees, drainage, block numbers, land ownership in outlying areas, selected public and industrial buildings, cemeteries, Parish boundaries, ferry routes, and more. Depths shown by soundings. Includes inset views, plans, and engravings: Perspective view of the buildings and grounds from the Northeast -- Mexican national headquarters -- Grand Rapids (Mich.) furniture pavilion -- [South pass] -- View of New Orleans in 1719 -- Railroad map of Louisiana and Texas -- Plan of New Orleans in 1770 by Capt.n Pittman of the British Army -- Ground plan -- United States and state exhibits -- Art gallery -- Main building -- Factories and Mills -- Horticultural hall. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Kansas City, Missouri, 1884 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Wright's map of Kansas City, compiled and drawn by Carl Gothe de Grote ; engraved by H.R. Page & Co. It was published by H.T. Wright in 1884. Scale [ca. 1:10,000]. Covers portions of Kansas City, Missouri, and Kansas City, Kansas. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Missouri West State Plane Coordinate System NAD83 (in Feet) (Fipszone 2403). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This is a partial cadastral map showing features such as roads, railroads and stations, drainage, selected public buildings, schools, churches, and industry locations (e.g. mills, factories, mines, etc.), names of property owners, and more. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

London, England : parishes, districts, and extra parochial places, 1884 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Map of London and its environs : shewing the boundary of the jurisdiction of the metropolitan board of work, also the boundaries of the city of London, the Parishes, the Districts & Extra Parochial Places. It was published by Edward Standford, April 21, 1884. Scale [ca. 1:31,680]. This map is part of a 5 map set showing various thematic districts and boundaries of the London region. The image inside the map neatline is georeferenced to the surface of the earth and fit to the British National Grid coordinate system (British National Grid, Airy Spheroid OSGB (1936) Datum). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads, drainage, selected private and public buildings, towns and villages, cemeteries, parks, farms, parish, district, and extra parochial place boundaries, and more. Relief is shown by hachures. Includes notes and indices. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.