Effect of Cage-Induced Stereotypies on Measures of Affective State and Recurrent Perseveration in CD-1 and C57BL/6 Mice.

Stereotypies are abnormal repetitive behaviour patterns that are highly prevalent in laboratory mice and are thought to reflect impaired welfare. Thus, they are associated with impaired behavioural inhibition and may also reflect negative affective states. However, in mice the relationship between stereotypies and behavioural inhibition is inconclusive, and reliable measures of affective valence are lacking. Here we used an exploration based task to assess cognitive bias as a measure of affective valence and a two-choice guessing task to assess recurrent perseveration as a measure of impaired behavioural inhibition to test mice with different forms and expression levels of stereotypic behaviour. We trained 44 CD- 1 and 40 C57BL/6 female mice to discriminate between positively and negatively cued arms in a radial maze and tested their responses to previously inaccessible ambiguous arms. In CD-1 mice (i) mice with higher stereotypy levels displayed a negative cognitive bias and this was influenced by the form of stereotypy performed, (ii) negative cognitive bias was evident in back-flipping mice, and (iii) no such effect was found in mice displaying bar-mouthing or cage-top twirling. In C57BL/6 mice neither route-tracing nor bar-mouthing was associated with cognitive bias, indicating that in this strain these stereotypies may not reflect negative affective states. Conversely, while we found no relation of stereotypy to recurrent perseveration in CD-1 mice, C57BL/6 mice with higher levels of route-tracing, but not bar-mouthing made more repetitive responses in the guessing task. Our findings confirm previous research indicating that the implications of stereotypies for animal welfare may strongly depend on the species and strain of animal as well as on the form and expression level of the stereotypy. Furthermore, they indicate that variation in stereotypic behaviour may represent an important source of variation in many animal experiments.

Compilation of results of the STRATAGEM project as ISO-image of the final CD-ROM

The study of the Neogene (Miocene to Holocene) stratigraphic record on the glaciated Atlantic margin of NW Europe has, to date, largely been undertaken on an ad-hoc basis. Whereas a systematic approach to understanding the stratigraphic development of Palaeogene and older strata has been undertaken in areas such as the North Sea, West of Shetland and Norway, the problem of establishing a Neogene framework has been only partly addressed by academia and the oil industry. In most cases where a Neogene stratigraphy has been constructed, this has been largely in response to problem solving and risk assessment in a restricted area. Nevertheless, in the past few years it has become increasingly apparent that there is a common history in the Neogene development of the passive Atlantic margin of NW Europe, between mid-Norway and SW Ireland. The inspection and interpretation of an extensive geophysical and geological database has identified several regionally significant and correlatable unconformities along this continental margin. Thus, a regional approach to the stratigraphical development of the Neogene succession on the glaciated European Atlantic margin is undertaken in this volume.