Cyclosporin A potentiates the dexamethasone-induced mouse mammary tumor virus-chloramphenicol acetyltransferase activity in LMCAT cells: a possible role for different heat shock protein-binding immunophilins in glucocorticosteroid receptor-mediated gene expression.

As previously observed for FK506, we report here that cyclosporin A (CsA) treatment of mouse fibroblast cells stably transfected with the mouse mammary tumor virus-chloramphenicol acetyltransferase (MMTV-CAT) reporter plasmid (LMCAT cells) results in potentiation of dexamethasone (Dex)-induced CAT gene expression. Potentiation by CsA is observed in cells treated with 10-100 nM Dex but not in cells treated with 1 microM Dex, a concentration of hormone which results in maximum CAT activity. At 10 nM Dex, 1-5 microM CsA provokes an approximately 50-fold increase in CAT gene transcription, compared with transcription induced by Dex alone. No induction of CAT gene expression is observed in cells treated with CsA or FK506 in the absence of Dex. The antisteroid RU 486 abolishes effects obtained in the presence of Dex. Using a series of CsA, as well as FK506, analogs, including some devoid of calcineurin phosphatase inhibition activity, we conclude that the potentiation effects of these drugs on Dex-induced CAT gene expression in LMCAT cells do not occur through a calcineurin-mediated pathway. Western-blotting experiments following immunoprecipitation of glucocorticosteroid receptor (GR) complexes resulted in coprecipitation of GR, heat shock protein hsp90 and two immunophilins: the FK506-binding protein FKBP59 and the CsA-binding protein cyclophilin 40 (CYP40). Two separate immunophilin-hsp90 complexes are present in LMCAT cells: one containing CYP40-hsp90, the other FKBP59-hsp90. Thus, both FKBP59 and CYP40 can be classified as hsp-binding immunophilins, and their possible involvement as targets of immunosuppressants potentiating the GR-mediated transcriptional activity is discussed.

Astrology in medicine : the Fitzpatrick lectures delivered before the Royal College of Physicians on November 6 and 11, 1913 : with addendum on saints and signs /

Mode of access: Internet.

Tabulae in grammaticen linguae Chaldaeae, quae & Syriaca dicitur : multa interim de rabbinico & Talmudico stilo traduntur : accessit ad calcem libellus De abbreuiaturis Hebraeorum, quibus & in Masora, & Talmudicis at que alijs scriptis passim vtuntur /

Mode of access: Internet.

The history of Canada : from its first discovery to the present time.

Mode of access: Internet.

Le crime de Guillaume II et la Belgique; récits d'un témoin oculaire.

Annexes: I. Deux proclamations allemandes en Belgique.--II. Une lettre de Maximilien Harden.--III. Le manifeste des intellectuels allemandes et les réponses venues de divers pays]--IV. La lettre pastorale du cardinal Mercier et les incidents qu'elle a provoqubes.--V. Extraits des rapports de la Commission belge d'enquête sur la violation des règles du droit des gens, des lois et des coutumes de la guerre.--Une lettre de M. Lafontaine.

Statesmen of the war in retrospect, 1918-1928 /

William II.--Francis-Joseph.--Nicholas II.--Pachitch.--Von Bethmann-Hollweg.--Count Tisza.--Poincaré.--Lord Grey.--Cardinal Mercier.--Baron Sonnino.--Venizelos.--Jon Bratiano.--Asquith.--Briand.--Hoover.--Gustave Ador.--President Wilson.--Colonel House.--Count Czernin.--President Masaryk and Dr. Bénèš.--Pilsudski and Paderewski.--Lloyd George.--Clémenceau.

Reports on the violation of the rights of nations and of the laws ans customs of war in Belgium.

[Vol. I] includes a preface by J. van den Heuvel, minister of state, reports 1-12, and extracts from the pastoral letter of Cardinal Mercier, archbishop of Malines; vol. II includes reports 13-22, with facsimiles of German soldiers' diaries, correspondence between Cardinal Mercier and the German authorities, the protest of Mgr. Heylen, bishop of Namur, etc., etc., appended.

Vie des martyrs, 1914-1916.

A travers le territoire.--Histoire de Carré et de Lerondeau.--Mémorial de la vie des martyrs.--Mort de Mercier.--A Verdun.--Le sacrifice.--La troisième symphonie.--La grace.--Nuits en Artois.

The new book of martyrs, from the French of Georges Duhamel,

Translation of Vie des martyrs.

La formation de l'État Tchécoslovaque,

Mode of access: Internet.

Histoire generale des auteurs sacrés et ecclesiastiques ... /

Mode of access: Internet.

Histoire generale des auteurs sacrés et ecclesiastiques ... /

Mode of access: Internet.

Histoire generale des auteurs sacrés et ecclesiastiques ... /

Mode of access: Internet.

Histoire generale des auteurs sacrés et ecclesiastiques ... /

Mode of access: Internet.