CLEC-2 expression is maintained on activated platelets and on platelet microparticles

The C-type lectin-like receptor CLEC-2 mediates platelet activation through a hem-immunoreceptor tyrosine-based activation motif (hemITAM). CLEC-2 initiates a Src- and Syk-dependent signaling cascade that is closely related to that of the 2 platelet ITAM receptors: glycoprotein (GP)VI and FcγRIIa. Activation of either of the ITAM receptors induces shedding of GPVI and proteolysis of the ITAM domain in FcγRIIa. In the present study, we generated monoclonal antibodies against human CLEC-2 and used these to measure CLEC-2 expression on resting and stimulated platelets and on other hematopoietic cells. We show that CLEC-2 is restricted to platelets with an average copy number of ∼2000 per cell and that activation of CLEC-2 induces proteolytic cleavage of GPVI and FcγRIIa but not of itself. We further show that CLEC-2 and GPVI are expressed on CD41+ microparticles in megakaryocyte cultures and in platelet-rich plasma, which are predominantly derived from megakaryocytes in healthy donors, whereas microparticles derived from activated platelets only express CLEC-2. Patients with rheumatoid arthritis, an inflammatory disease associated with increased microparticle production, had raised plasma levels of microparticles that expressed CLEC-2 but not GPVI. Thus, CLEC-2, unlike platelet ITAM receptors, is not regulated by proteolysis and can be used to monitor platelet-derived microparticles.

Characterization of anodic silicon oxide films grown in room temperature ionic liquids

The electroformation of silicon oxide was performed in two room temperature ionic liquids (RTIL), 1-butyl-3-methyl-imidazolium bis(trifluoromethane sulfonyl) imide (BMITFSI) and N-n-butyl-N-methylpiperidinium bis(trifluoromethane sulfonyl) imide (BMPTFSI). This phenomenon was studied by electrochemical techniques and it was observed that the oxide growth follows a high-field mechanism. X-ray Photoelectron Spectroscopy experiments have shown that a non-stoichiometric oxide film was formed, related to the low water content present in both RTILs (< 30 ppm). The roughness values obtained by using AFM technique of the silicon surface after etching with HF was 1.5 nm (RMS). The electrochemical impedance spectroscopy at low frequencies range was interpreted as a resistance in parallel with a CPE element, the capacitance obtained was associated with the dielectric nature of the oxide formed and the resistance was interpreted considering the chemical dissolution of the oxide by the presence of the TFSI anion. The CPE element was associated with the surface roughness and the very thin oxide film obtained. (C) 2007 Elsevier Ltd. All rights reserved.

Immobilization of Catalysts of Biological Interest on Porous Oxidized Silicon Surfaces

The present paper deals with the immobilization of redox mediators and proteins onto protected porous silicon surfaces to obtain their direct electrochemical reactions and to retain their bioactivities. This paper shows that MP-11 and viologens are able to establish chemical bonds with 3-aminopropyltriethoxylsilane-modified porous silicon surface. The functionalization of the surfaces have been fully characterized by energy dispersive X-ray analysis (EDX) and X-ray photoelectron spectroscopy (XPS) to examine the immobilization of these mediators onto the solid surface. Amperometric and open circuit potential measurements have shown the direct electron transfer between glucose oxidase and the electrode in the presence of the viologen mediator covalently linked to the 3-aminopropyltriethoxylsilane (APTES)-modified porous silicon surfaces.

Utveckling av en ny modul i WM-datas arbetsorderhanteringssystem för registrering av snöröjning respektive sandning

WM-data tillhandahåller en lösning för mobil arbetsorderhantering. Lösningen innehåller planeringsverktyg med ärenden, personal och karta och möjlighet att leverera arbetsorder till fältpersonal. Den innehåller dessutom handhållna mobilenheter, vilka fältpersonalen använder för att ta emot aktuella arbetsorder.Vissa kunder önskar ytterligare en modul i denna mobila lösning. Den nya modulen skall användas för att kontinuerligt rapportera in genomförda åtgärder i snöröjningssammanhang.Detta examensarbete går ut på att utveckla den här modulen för att registrera snöröjning respektive sandning. Applikationen exekveras på en PDA, en så kallad Pocket PC med Windows Mobile 5.0. Anledningen till att just Windows Mobile 5.0 skall användas är att detta operativsystem har inbyggt stöd för GPS-positionering. Ett önskemål av WM-data var att detta inbyggda stöd skulle undersökas, rapporteras och användas i applikationen.Resultatet blev en applikation där man via en Pocket PC kontinuerligt sparar undan fordons GPS-position, arbetsåtgärd samt tidpunkterna när GPS-positionerna togs. Denna information är sedan anpassad för att kunna skickas vidare via GPRS till en befintlig databasserver under vissa tidsintervaller.Applikationen utvecklades med hjälp av programmeringsspråket C Sharp (C#) i Visual Studio 2005.

Estudio intertextual en dos cuentos de Borges : Pierre Menard, autor del Quijote y La biblioteca de Babel

This studyfocuses on the theory of intertextuality and on the most important approachesof Julia Kristeva, Gerard Genette and Ronald Barthestothis theory. It also examines the intertextual relationships in twoworksof Jorge Luis Borges:Pierre Menard, author of the Quixoteand The library of Babel. This studyconcludes that intertextual relations and issues are very often used in the works of Jorge Luis Borges. Analysis of histwoworks has shown the most obvious indicators of intertextuality such as allusions, quotations and references.

Comparison Of Demand Pattern Calibration In Water Distribution Networks With Geographic And Non-Geographic Parameterization

Demands are one of the most uncertain parameters in a water distribution network model. A good calibration of the model demands leads to better solutions when using the model for any purpose. A demand pattern calibration methodology that uses a priori information has been developed for calibrating the behaviour of demand groups. Generally, the behaviours of demands in cities are mixed all over the network, contrary to smaller villages where demands are clearly sectorised in residential neighbourhoods, commercial zones and industrial sectors. Demand pattern calibration has a final use for leakage detection and isolation. Detecting a leakage in a pattern that covers nodes spread all over the network makes the isolation unfeasible. Besides, demands in the same zone may be more similar due to the common pressure of the area rather than for the type of contract. For this reason, the demand pattern calibration methodology is applied to a real network with synthetic non-geographic demands for calibrating geographic demand patterns. The results are compared with a previous work where the calibrated patterns were also non-geographic.

The network of intertextual relations in Naipaul's half a life and magic seeds

Esta dissertação mapeia a rede de relações intertextuais em Half a Life (2001) e sua continuação Magic Seeds (2004), os romances mais recentes do Prêmio Nobel de Literatura de 2001, V. S. Naipaul, como contribuição para o estudo da obra do autor. A noção de intertextualidade permeia os estudos literários, e o termo tem sido largamente empregado desde que foi cunhado por Julia Kristeva nos anos sessenta. Desde então as mais variadas, e muitas vezes divergentes, teorias sobre intertextualidade compartilham a idéia de que um texto só adquire significado pleno na interação com outros textos. A abordagem metodológica proposta é baseada na teoria da transtextualidade de Gérard Genette. Esta escolha implica o estudo de intertextos, paratextos, metatextos, arquitextos e hipertextos que constituem a interface entre os dois romances e outros escritos. O nome do protagonista "William Somerset Chandran" constitui o fio que guia o estudo das várias relações transtextuais nos dois romances. A partir do prenome do protagonista – William – este estudo situa os romances no contexto da tradição do Bildungsroman, e argumenta que estes estabelecem uma paródia arquitextual do gênero na medida em que subvertem seu cerne, ou seja, a formação do caráter do protagonista. O nome do meio do protagonista – Somerset – remete à ficcionalização do escritor Somerset Maugham na narrativa, ao mesmo tempo em que esta desmistifica a ótica ocidental sobre o hinduísmo popularizada por Maugham em The Razor's Edge. O sobrenome do protagonista – Chandran – leva ao estudo do conjunto de referências à origem indiana de Naipaul e o papel desta na produção do autor. Este nome se reporta ao romance de Narayan The Bachelor of Arts, cujo protagonista também é nomeado Chandran. Narayan é um escritor de destaque na literatura anglo-indiana e referência recorrente na obra de Naipaul. Os temas de migração e choque cultural apresentados nos dois romances têm sido presença constante na obra de Naipaul. Esta pesquisa mapeia a relação de continuidade entre os dois romances em questão e o conjunto da obra de Naipaul, salientando o papel da ambientação geográfica da narrativa, marcada pela jornada do protagonista através de três continentes. A teoria da transtextualidade é uma ferramenta operacional para a pesquisa, a qual examina a densidade das referências geográficas, históricas e literárias em Half a Life e Magic Seeds, visando aportar elementos para o estudo da produção literária de Naipaul, na medida em que estes romances recentes condensam e revisitam a visão de mundo deste autor.

OpenWordNet-PT: an open brazilian wordnet for reasoning

Brazilian Portuguese needs a Wordnet that is open access, downloadable and changeable, so that it can be improved by the community interested in using it for knowledge representation and automated deduction. This kind of resource is also very valuable to linguists and computer scientists interested in extracting and representing knowledge obtained from texts. We discuss briefly the reasons for a Brazilian Portuguese Wordnet and the process we used to get a preliminary version of such a resource. Then we discuss possible steps to improving our preliminary version.

Transfer function-noise modeling and spatial interpolation to evaluate the risk of extreme (shallow) water-table levels in the Brazilian Cerrados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Do teatro Elisabetano ao sertão do século XIX: a presença de Shakespeare em Inocência

The Shakespearean theater is a result of the genius of this playwright alongside the material provided by the period in which they came out the Elizabethan Age. Most of his works bring up themes and elements which keep them up-to-date, arousing an ongoing interest of readers and theatergoers, and also serving as inspiration for other writers to create their own works. Taking these ideas into account, this work aims to bring up questions concerning the presence of Shakespeare in a nineteenth-century Brazilian novel, Inocência, by Viscount of Taunay. In this novel, Taunay makes references to this playwright, using some epigraphs taken from Romeo and Juliet, from which we seek to understand how the novel dialogues with this Shakespearean drama. In order to develop such a study, we take into account some theoretical assumptions of hypertextuality, as proposed by the French scholar Gerard Genette, whose ideas about the dialogue between literary works support the analysis of the relationship between Taunay s novel and the above-referenced play of Shakespeare.

A complexidade do ser fictício: a construção da personagem Marcela no romance A ostra e o vento

It is possible to notice that the modern narrative increasingly seeks to invest its characters with a broad and complex nature, away from the well-defined beings of the traditional narrative. Through this work, we aim to make a study of Marcela, a character of the novel A ostra e o vento, by Moacir Costa Lopes, taking into account the way the complexity of the fictitious being is constituted through an analysis of structural, semantic, and thematic elements. We first will bring up a brief theoretical discussion about the character in a novel, and we also will carefully analyze the diagetic universe, presenting the complex path of the character further. Subsequently, we will focus on the structure of the narrative which creates a complex picture of the character, using the technique of crossed points of view as well as the technique of temporal fragmentation. Finally, we will investigate conflicting social relations that portray Marcela s disturbed inner side, as well as the metaphoric symbolic language, which furnishes a number of different representation of this character, impeding the creation of a well-structured coherent character. Studies of scholars such as Antonio Candido, Anatol Rosenfeld, Vitor Manuel de Aguiar e Silva, Fernando Segolin, Gerard Genette, Michel Zéraffa, among others, will guide our analysis

Collider aspects of flavor physics at high Q

This chapter of the "Flavor in the era of LHC" workshop report discusses flavor-related issues in the production and decays of heavy states at the LHC at high momentum transfer Q, both from the experimental and the theoretical perspective. We review top quark physics, and discuss the flavor aspects of several extensions of the standard model, such as supersymmetry, little Higgs models or models with extra dimensions. This includes discovery aspects, as well as the measurement of several properties of these heavy states. We also present publicly available computational tools related to this topic.

FÉCULA E FARELO DE MANDIOCACOMO SUBSTRATO NA PRODUÇÃO DE CICLODEXTRINAS

Ciclodextrinas (CDs) são oligossacarídeos cíclicos, não redutores, capazes de formar complexos de inclusão com outras moléculas, modificando suas características químicas e físicas. São de interesse industrial, mas o fator limitante para sua utilização ainda é o alto custo de produção. Devido à sua pureza e ao teor de amilopectina, a fécula de mandioca se apresenta como substrato potencial para a produção de CDs, tendo mais de 95% de amido. Outro substrato potencial é o farelo, resíduo da extração da fécula de mandioca, com cerca de 70% de amido e custo consideravelmente inferior ao da fécula. O objetivo deste trabalho foi avaliar a produção de CDs usando fécula e farelo de mandioca como substratos, empregando ciclodextrina glucosiltransferase (CGTase; E. C. 2.4.1.19) proveniente de Bacillus macerans. A conversão do amido em a-CDs foi de 19% para a fécula e 21% para o farelo. Para b-CDs, os valores foram de 27% e 15% para a fécula e o farelo, respectivamente. A proporção de a: b-CDs produzidas a partir da fécula foi de 1,0:1,4, enquanto que para o farelo foi de 1,5:1,0. Após 4 horas a 50ºC houve considerável perda da atividade enzimática, indicando tendência de estabilização da reação.

GDP 4.0 transfer to SGP 3.0 for SCIAMACHY no2 column processing: Verification with SDOAS / GDOAS prototype algorithms and delta-validation with NDACC / UV-visible network data

Until mid 2006, SCIAMACHY data processors for the operational retrieval of nitrogen dioxide (NO2) column data were based on the historical version 2 of the GOME Data Processor (GDP). On top of known problems inherent to GDP 2, ground-based validations of SCIAMACHY NO2 data revealed issues specific to SCIAMACHY, like a large cloud-dependent offset occurring at Northern latitudes. In 2006, the GDOAS prototype algorithm of the improved GDP version 4 was transferred to the off-line SCIAMACHY Ground Processor (SGP) version 3.0. In parallel, the calibration of SCIAMACHY radiometric data was upgraded. Before operational switch-on of SGP 3.0 and public release of upgraded SCIAMACHY NO2 data, we have investigated the accuracy of the algorithm transfer: (a) by checking the consistency of SGP 3.0 with prototype algorithms; and (b) by comparing SGP 3.0 NO2 data with ground-based observations reported by the WMO/GAW NDACC network of UV-visible DOAS/SAOZ spectrometers. This delta-validation study concludes that SGP 3.0 is a significant improvement with respect to the previous processor IPF 5.04. For three particular SCIAMACHY states, the study reveals unexplained features in the slant columns and air mass factors, although the quantitative impact on SGP 3.0 vertical columns is not significant.