Evidence for a QTL on chromosome 19 influencing LDL cholesterol levels in the general population

The genetic basis of cardiovascular disease (CVD) with its complex etiology is still largely elusive. Plasma levels of lipids and apolipoproteins are among the major quantitative risk factors for CVD and are well-established intermediate traits that may be more accessible to genetic dissection than clinical CVD end points. Chromosome 19 harbors multiple genes that have been suggested to play a role in lipid metabolism and previous studies indicated the presence of a quantitative trait locus (QTL) for cholesterol levels in genetic isolates. To establish the relevance of genetic variation at chromosome 19 for plasma levels of lipids and apolipoproteins in the general, out-bred Caucasian population, we performed a linkage study in four independent samples, including adolescent Dutch twins and adult Dutch, Swedish and Australian twins totaling 493 dizygotic twin pairs. The average spacing of short-tandem-repeat markers was 6 - 8 cM. In the three adult twin samples, we found consistent evidence for linkage of chromosome 19 with LDL cholesterol levels ( maximum LOD scores of 4.5, 1.7 and 2.1 in the Dutch, Swedish and Australian sample, respectively); no indication for linkage was observed in the adolescent Dutch twin sample. The QTL effects in the three adult samples were not significantly different and a simultaneous analysis of the samples increased the maximum LOD score to 5.7 at 60 cM pter. Bivariate analyses indicated that the putative LDL-C QTL also contributed to the variance in ApoB levels, consistent with the high genetic correlation between these phenotypes. Our study provides strong evidence for the presence of a QTL on chromosome 19 with a major effect on LDL-C plasma levels in outbred Caucasian populations.

Texto para discuss??o 16: novos padr??es gerenciais no setor p??blico: medidas do governo americano orientadas para o desempenho e resultados

O estudo realizado envolve um benchmarking de novos padr??es gerenciais adotados pelo governo dos Estados Unidos, notadamente no que se refere a formas inovadoras de gest??o, de controle de gest??o, de gest??o or??ament??ria e de avalia????o institucional. Para a realiza????o do presente trabalho, os autores valeram-se de consulta bibliogr??fica a livros e documentos produzidos nos ??ltimos cinco anos, cabendo destacar, dentre os diversos documentos citados nas refer??ncias bibliogr??ficas e notas, os estudos produzidos pela National Academy of Public Administration ??? NAPA e pelo General Accounting Office ??? GAO sobre o processo de ???reinven????o do governo??? americano. Integram, tamb??m, o presente trabalho informa????es obtidas atrav??s de participa????o em semin??rios e palestras conduzidas pela NAPA e pela Virginia Polytechnic Institute and State University, no per??odo de 03 a 14 de junho de 1996, no ??mbito de projeto de Avalia????o de Programas P??blicos Orientada para Resultados, desenvolvido pela Escola Brasileira de Administra????o P??blica da Funda????o Get??lio Vargas (EBAP/FGV) para o Tribunal de Contas da Uni??o (TCU) do Brasil. Tais palestras inclu??ram, dentre outros assuntos, a vis??o geral da avalia????o no ??mbito do governo federal americano; o papel do Office of Management and Budget (OMB) na avalia????o e mensura????o de desempenho; a evolu????o da auditoria interna e externa, bem como a avalia????o de programas efetuada pela Rand Corporation. Finalmente, ainda no ??mbito do programa referido, foram utilizados materiais e informa????es coletadas a partir de entrevistas efetuadas em organiza????es governamentais como, dentre outras, o U.S. Department of Health and Human Services, e em organiza????es particulares que realizam avalia????o de programas, por for??a de contratos de presta????o de servi??os, como o Urban Institute, a Mathematic Policy Research e a Maximus, todas situadas em Washington D.C

Forty Years of the United Nations General Assembly Resolution 3379 (XXX) on Zionism and Racism: the Brazilian Vote as an instance of United States - Brazil Relations

Abstract In 1975, Brazil voted in favor of the United Nations General Assembly resolution 3379 (XXX), equating Zionism with a form of racism. Focusing on the decision-making process of president Ernesto Geisel's (1974-1979) foreign policy, "responsible pragmatism", this article discusses how the ultimate decision to vote in favor of resolution was taken taking into account mainly US-Brazil relationship.

Building the Directorate General for Urban Affairs in European Union

LUDA is a research project of Key Action 4 "City of Tomorrow & Cultural Heritage" of the programme "Energy, Environment and Sustainable Development" within the Fifth Framework Programme of the European Commission

A high-throughput screening method for general cytotoxicity part I Chemical toxicity

Foi desenvolvido um método destinado a fazer a triagem rápida e o escalonamento da toxicidade geral exercida por xenobióticos tendo como modelo o Saccharomyces cerevisiae. Para padronizar as condições de experimentação foi estabelecida a relação entre a absorvência a 525 nm e o número de células em suspensão por mililitro de meio de cultura e calculadas uma curva padrão e respectiva equação definidora (Y=6,8219E-08X + 0,0327) Culturas de Saccharomyces cerevisiae em meio completo para leveduras (YPD - 1% de glucose 2%, de peptona 0,5% e extracto de levedura 1%) foram expostas a diferentes concentrações de nicotina e a inibição do crescimento avaliada.

General Insights into Word Choice in Business Communication

Words are the smallest units of messages. Attention should be given to each word used to be sure it is the most effective one. An effective word is one that the receiver will understand and that will elicit the wanted response. The ability to choose words by (a) using a dictionary and a thesaurus and (b) following some of the principles of business communication described in this text can be improved.

General Ribeiro Arthur militar e artista

Ribeiro Arthur foi militar, crítico de Arte e foi sobretudo um dos mais importantes investigadores e ilustradores da história dos uniformes em Portugal. Produziu um número muito significativo de aguarelas dedicadas a este tema que, reunidas em colecção, estão preservadas no Arquivo Histórico Militar. ABSTRACT - Artur Ribeiro Arthur was military, art critic and was one of the most important researchers and illustrators in the history of the uniforms in Portugal. He produced a significant number of watercolors dedicated to this theme, gathered in collections that are kept in the Military Historical Archive.

Epidemiología de la enfermedad de Chagas, Departamento General Paz, Argentina

OBJETIVO: El objetivo del presente trabajo fue investigar la colonización por triatominos en ambientes domésticos y peridomésticos y evaluar la seroprevalencia de infectados chagásicos en localidades rurales. MÉTODOS: La investigación se realizó en General Paz, Corrientes, Argentina. Las viviendas y peridomicilios se seleccionaron mediante un muestreo simple al azar. La búsqueda de triatominos se efectuó por el método captura/hora/hombre. Los insectos se identificaron taxonómicamente y se clasificaron según sexo y edad. La infección por Trypanosoma cruzi se detectó por observación directa de las heces al microscopio y por la técnica de la reacción en cadena de la polimerasa (PCR). Se evaluaron serológicamente a pobladores voluntarios mediante las técnicas de hemaglutinación indirecta (HAI) y ensayo inmunoenzimático (ELISA). Se hallaron los índices de infestación, densidad, colonización, infección natural y dispersión. RESULTADOS: Se analizaron 42 viviendas y 50 peridomicilios. En el domicilio los índices de infestación e infección de Triatoma infestans fueron 23,8 y 19,4 respectivamente. Los índices de densidad, colonización y dispersión fueron 2,1; 47,0 y 50,0 respectivamente. La infestación del peridomicilio por T. infestans fue 5,9% y por T. sordida 11,8%. T. sordida resultó infectada por Trypanosoma cruzi en un 2,0%. La seroprevalencia al T. cruzi de los 85 pacientes fue del 22,3%. CONCLUSIONES: Se estima que en el área investigada la transmisión del T. cruzi está interrumpida. Sin embargo, la presencia de infectados humanos y de T. infestans parasitadas posibilitan el reinicio del ciclo de transmisión. Se proponen acciones de rociado con insecticidas con el objeto de eliminar las poblaciones del vector.

Factores asociados a mortalidad perinatal en el hospital general de Chiapas, México

OBJETIVO: El objetivo del estudio es identificar factores socioeconómicos, gineco-obstétricos y del producto asociados a mortalidad perinatal. MÉTODOS: Se realizó un estudio de casos y controles pareado. Se consideró caso a los nacidos vivos o muertos que nacieron y fallecieron entre las 28 semanas de gestación a los 7 días de vida extrauterina. y control al producto nacido vivo entre las 28 semanas de gestación y los 7 días de vida extrauterina. Los datos se obtuvieron de los expedientes clínicos hospitalarios. Se estudiaron 99 casos y 197 controles. Se hizo un análisis estadístico utilizando Stata 6.0. RESULTADOS La media de edad de la madre fue de 24.82 años y del producto de 37.78 semanas de gestación. El promedio de peso del producto fue de 2,760 gramos. Los factores asociados a mortalidad perinatal fueron: ocupación del padre agricultor (RM ajustada 3,31; IC 95% 1,26-8,66); índice de riesgo obstétrico alto (RM ajustada 10,57; IC 95% 2,82-39,66), antecedente de cesárea (RM ajustada 2,75; IC 95% 1,37-5,51); cinco y más consultas prenatales (RM ajustada 4,43; IC 95% 1.86-10,54); producto pretérmino (RM ajustada 9,20; IC 95% 4,39-19,25). CONCLUSIONES: Los resultados muestran que es necesario implementar medidas de prevención y control que aseguren la identificación del riesgo en las mujeres embarazadas, con el fin de abatir la incidencia de mortalidad perinatal.

Paranoia in the General Population : a revised version of the General Paranoia Scale for Adults

Background: Paranoid ideation has been regarded as a cognitive and a social process used as a defence against perceived threats. According to this perspective, paranoid ideation can be understood as a process extending across the normal-pathological continuum. Methods: In order to refine the construct of paranoid ideation and to validate a measure of paranoia, 906 Portuguese participants from the general population and 91 patients were administered the General Paranoia Scale (GPS), and two conceptual models (one - and tridimensional) were compared through confirmatory factor analysis (CFA). Results: Results from the CFA of the GPS confirmed a different model than the one-dimensional model proposed by Fenigstein and Vanable, which com-prised three dimensions (mistrust thoughts, persecutory ideas, and self-deprecation). This alternative model presented a better fit and increased sensitivity when compared with the one-dimensional model. Further data analysis of the scale revealed that the GPS is an adequate assessment tool for adults, with good psychometric characteristics and high internal consistency. Conclusion: The model proposed in the current work leads to further refinements and enrichment of the construct of paranoia in different populations, allowing the assessment of three dimensions of paranoia and the risk of clinical paranoia in a single measure for the general population.

Paranoia in the General Population : a revised version of the General Paranoia Scale for adolescents

The aim of the current study was to validate the General Paranoia Scale for Portuguese Adolescents population (GPS-A). This scale assesses the paranoid ideation in non-clinical population. Results from a confirmatory factor analysis of the scale on 1218 youths confirmed an alternative model to the one-dimensional model proposed by Fenigstein and Vanable (1992) comprising three different dimensions (Mistrust thoughts, persecutory ideas and depreciation). This alternative model presented a good fit: χ2 (162)= 727.200, p = .000; CFI = .925; RMSEA = .054, P(rmsea ≤0.05) = .000; PCFI = .788; AIC = 863.200. All items presented adequate factor loadings (λij ≥0.5) and individual reliability ((λij)2 ≥0.25). Further data analysis on the scale revealed that the GPS-A is an adequate assessment tool for adolescents, with good psychometric characteristics and high internal consistency.

The economics of language or how can language be an asset? General reflections on the European case and brief focus on the case of portuguese

In this paper, we will focus on the importance of languages as an asset to people and companies in knowledge-based society, giving special attention to the case of portuguese, not forgetting the role of Higher Education Institutions in preparing students to be part of the new creative multilingual and sucsessful class.