Lipase production by marine fungus Aspergillus Awamori Nagazawa

The present study indicate the scope for the utilization of the marine fungus Aspergillus awamori Nagazawa BTMFW 032 for extracellular lipase production employing submerged fermentation. To the best of our knowledge this is the first report on lipase production by a marine fungus employing statistical modeling towards industrial production. The characterization of purified lipase produced by A. awamori showed stability in organic solvents, oxidizing agent and reducing agents, I,3-regiospecificity and hydrolytic activity. These properties make this lipase an ideal candidate for biocatalysis in organic media for the production of novel compounds such as biodiesel and sugar fatty esters. 91.4 % reduction in oil and grease content in ayurvedic oil by the treatment of A. awamori lipase indicates that there is a scope for this enzyme in the treatment of oil effluents and bioremediation. There is ample scope for further research on the biochemistry of the enzyme, structure elucidation and enzyme engineering towards a wide range of further applications, besides enriching scientific knowledge on marine enzymes.

Molecular cloning of alkaline protease gene from marine fungus Engyodontium album

Department of Biotechnology, Cochin University of Science and Technology

Molecular cloning and homology modelling of a subtilisin-like serine protease from the marine fungus, Engyodontium album BTMFS10

An alkaline protease gene (Eap) was isolated for the first time from a marine fungus, Engyodontium album. Eap consists of an open reading frame of 1,161 bp encoding a prepropeptide consisting of 387 amino acids with a calculated molecular mass of 40.923 kDa. Homology comparison of the deduced amino acid sequence of Eap with other known proteins indicated that Eap encode an extracellular protease that belongs to the subtilase family of serine protease (Family S8). A comparative homology model of the Engyodontium album protease (EAP) was developed using the crystal structure of proteinase K. The model revealed that EAP has broad substrate specificity similar to Proteinase K with preference for bulky hydrophobic residues at P1 and P4. Also, EAP is suggested to have two disulfide bonds and more than two Ca2? binding sites in its 3D structure; both of which are assumed to contribute to the thermostable nature of the protein.

Utilization of prawn waste for chitinase production by the marine fungus Beauveria bassiana by solid state fermentation

Prawn waste, a chitinous solid waste of the shell®sh processing industry, was used as a substrate for chitinase production by the marine fungus Beauveria bassiana BTMF S10, in a solid state fermentation (SSF) culture. The process parameters in¯uencing SSF were optimized. A maximum chitinase yield of 248.0 units/g initial dry substrate (U/gIDS) was obtained in a medium containing a 5:1 ratio (w/v) of prawn waste/sea water, 1% (w/w) NaCl, 2.5% (w/w) KH2PO4, 425±600 lm substrate particle size at 27 °C, initial pH 9.5, and after 5 days of incubation. The presence of yeast extract reduced chitinase yield. The results indicate scope for the utilization of shell®sh processing (prawn) waste for the industrial production of chitinase by using solid state fermentation.

Molecular cloning and homology modelling of a subtilisin-like serine protease from the marine fungus, Engyodontium album BTMFS10

An alkaline protease gene (Eap) was isolated for the first time from a marine fungus, Engyodontium album. Eap consists of an open reading frame of 1,161 bp encoding a prepropeptide consisting of 387 amino acids with a calculated molecular mass of 40.923 kDa. Homology comparison of the deduced amino acid sequence of Eap with other known proteins indicated that Eap encode an extracellular protease that belongs to the subtilase family of serine protease (Family S8). A comparative homology model of the Engyodontium album protease (EAP) was developed using the crystal structure of proteinase K. The model revealed that EAP has broad substrate specificity similar to Proteinase K with preference for bulky hydrophobic residues at P1 and P4. Also, EAP is suggested to have two disulfide bonds and more than two Ca2? binding sites in its 3D structure; both of which are assumed to contribute to the thermostable nature of the protein.

Church-Rosser groups and growing context-sensitive groups

A finitely generated group is called a Church-Rosser group (growing context-sensitive group) if it admits a finitely generated presentation for which the word problem is a Church-Rosser (growing context-sensitive) language. Although the Church-Rosser languages are incomparable to the context-free languages under set inclusion, they strictly contain the class of deterministic context-free languages. As each context-free group language is actually deterministic context-free, it follows that all context-free groups are Church-Rosser groups. As the free abelian group of rank 2 is a non-context-free Church-Rosser group, this inclusion is proper. On the other hand, we show that there are co-context-free groups that are not growing context-sensitive. Also some closure and non-closure properties are established for the classes of Church-Rosser and growing context-sensitive groups. More generally, we also establish some new characterizations and closure properties for the classes of Church-Rosser and growing context-sensitive languages.

Cassava root peel as a replacement for maize in diets for growing pigs: effects on energy and nutrient digestibility, performance and carcass characteristics

Two experiments were conducted to evaluate cassava root peel (CRP) as diet component for fattening pigs. In the first experiment, ten male pigs were used to investigate the nutrient digestibility and the nutritive value of CRP as replacement for maize in the diet at 0 %, 30 %, 40 %, 50 % and 60 %, while supplementing free amino acids (fAA). During two experimental periods, faeces were quantitatively collected and analysed for chemical composition. In the second experiment, 40 pigs received the same diets as in Experiment 1, and daily feed intake and weekly weight changes were recorded. Four pigs per diet were slaughtered at 70 kg body weight to evaluate carcass traits. Digestibility of dry and organic matter, crude protein, acid detergent fibre and gross energy were depressed (p<0.05) at 60 % CRP; digestible energy content (MJ kg^(−1) DM) was 15.4 at 0 % CRP and 12.7 at 60 % CRP. In the second experiment, CRP inclusion had only a small impact on feed intake, weight gain and feed conversion ratio (p>0.05) as well as on the length of the small intestine and the Longissimus dorsi muscle area. The missing correlation of daily weight gain and feed-to-gain ratio up to a CRP inclusion of 40 % indicates that negative effects of CRP on pig growth can be avoided by respecting upper feeding limits. Hence, a combined use of CRP and fAA can reduce feeding costs for small-scale pig farmers in countries where this crop-by product is available in large amounts.

Growing through Connections – A Multi-Case Study of Two Alternative Food Networks in Cluj-Napoca, Romania

In a context of urgent global socio-ecological challenges, the aim of this paper has been to explore the potential of localised and socially connected food systems. More specifically, through a multi-case study of two alternative food networks in the city of Cluj-Napoca, Romania, their contribution to a sustainable food paradigm has been explored. An important synergy within the networks is how good food is equated with peasant produce, but issues regarding quantity, delivery arrangement, power relations and inclusiveness constitute potential conflicts. Although challenged by unfavourable trends on national and EU levels, the networks are becoming more embedded horizontally, through an intrinsic focus on community in one case and through quality food stimulating good relations in the other case. The networks contribute to a sustainable food paradigm by promoting agroecology, by reclaiming socio-cultural factors of food provisioning and by being part of a (re)-peasantisation process. Exploring how these kinds of initiatives can emerge, be sustained and be developed is of relevance, especially considering their potential for improving the prospects of environmentally sustainable and socially just futures in Romania and beyond.

A concurrent region growing algorithm guided by circumscribed contours

Image segmentation of natural scenes constitutes a major problem in machine vision. This paper presents a new proposal for the image segmentation problem which has been based on the integration of edge and region information. This approach begins by detecting the main contours of the scene which are later used to guide a concurrent set of growing processes. A previous analysis of the seed pixels permits adjustment of the homogeneity criterion to the region's characteristics during the growing process. Since the high variability of regions representing outdoor scenes makes the classical homogeneity criteria useless, a new homogeneity criterion based on clustering analysis and convex hull construction is proposed. Experimental results have proven the reliability of the proposed approach

A new approach to segmentation based on fusing circumscribed contours, region growing and clustering

One of the major problems in machine vision is the segmentation of images of natural scenes. This paper presents a new proposal for the image segmentation problem which has been based on the integration of edge and region information. The main contours of the scene are detected and used to guide the posterior region growing process. The algorithm places a number of seeds at both sides of a contour allowing stating a set of concurrent growing processes. A previous analysis of the seeds permits to adjust the homogeneity criterion to the regions's characteristics. A new homogeneity criterion based on clustering analysis and convex hull construction is proposed

Ants.

Se pretende enseñar a los niños el ciclo de vida de las hormigas y la forma en que interactúan con su entorno, sus hábitos, su estilo de vida y las amenazas a los que se enfrenta por la actuación de los hombres al modificar las condiciones ambientales. La obra contiene un mapa de distribución del hábitat de las hormigas, glosario y bibliografía.

Can Earth support our growing population?

Para conocer las causas y consecuencias de la explosión demográfica. Invita a los lectores a pensar y expresarse de manera independiente. Incluye actividades que fomentan el pensamiento crítico y creativo para evaluar diferentes perspectivas sobre las cuestiones relacionadas con la población y la distribución de recursos. Para animar al debate se ofrece estudios de casos, relatos, informes, discusiones.

101 more life skills games for children : learning, growing, getting along (ages 9 to 15).

Recurso para todo lo que se refiere a grupos de niños, ya sea en la escuela, en la guardería, o simplemente la hora de organizar una reunión de juego no estructurado. Los juegos son una manera ideal para ayudar a los niños a desarrollar habilidades sociales y emocionales. Cada juego tiene claramente especificados los objetivos, sugerencias de variaciones, y un conjunto de preguntas que ayudan al grupo a reflexionar sobre las cuestiones abordadas en el juego. Los juegos cuentan con la improvisación, la pantomima, juegos de rol, de relajación, de competencia y de cooperación. También hay de movimiento, de lenguaje y juegos de adivinanzas, juegos de corro que ayudan a niños de 9 a 15 años a entender los problemas y necesidades que surgen en la interacción diaria con otros niños.

101 life skills games for children : learning, growing, getting along (ages 6 to 12).

Recurso para todo lo que se refiere a grupos de niños, ya sea en la escuela, en la guardería, o simplemente la hora de organizar una reunión de juego no estructurado. Los juegos son una manera ideal para ayudar a los niños a desarrollar habilidades sociales y emocionales. Cada juego tiene claramente especificados los objetivos, sugerencias de variaciones, y un conjunto de preguntas que ayudan al grupo a reflexionar sobre las cuestiones abordadas en el juego. Los juegos cuentan con la improvisación, la pantomima, juegos de rol, de relajación, de competencia y de cooperación. También hay de movimiento, de lenguaje y juegos de adivinanzas, juegos de corro que ayudan a niños de 6 a 12 años a entender los problemas y necesidades que surgen en la interacción diaria con otros niños.

Effect of Community of Residence on Neurobehavioral Development in Infants and Young Children in a Flower-Growing Region of Ecuador

In this study we compared neurobehavioral development in Ecuadoran children living in two communities with high potential for exposure to organophosphate (OP) and carbamate pesticides to that of children living in a community with low potential for exposure. Residence in communities with high potential for exposure to OP and carbamate pesticides was associated with poorer neurobehavioral development of the child even after controlling for major determinants of delayed development. Malnourished populations may be particularly vulnerable to neurobehavioral effects of pesticide exposure.