Heart rate variability in children with type 1 diabetes mellitus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Alterações da matriz extracelular causadas pelo diabetes: o impacto sobre a continência urinária

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effects of therapeutic approach on the neonatal evolution of very low birth weight infants with patent ductus arteriosus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Perfil comunicativo de crianças que ingressaram na educação infantil após os cinco anos de idade

Objective: To characterize the communicative behaviors of children who entered in Primary School after the age of five. Methods: It was a descriptive study, held in a city at the countryside of Parana state (Brazil), in the years 2007 and 2008, in both school and home environments. Twelve children of both genders joined in the study, with age ranging from five years and two months to six years, besides their mothers and respective teachers. Interviews were conducted with mothers and a questionnaire for teachers. Afterwards, observations of the communication of children in school and home environments were conducted. Data analysis focused on those relevant to children’s development and school performance, trying to determine their communicative profile in school and home environments, from protocols regarding the indicators of communication means and functions. Results: The results indicated the presence of a child with poor general health, with complications from the pregnancy period, with motor and language delays and low school performance. It was also observed that all children used verbal means to communicate and the highest frequency of communicative functions appeared in home environment, indicating an important aspect to be considered in the stimulation of communication of these children. Conclusion: Data from this study showed that the children benefited more of contexts planned with family interlocutors.

Estresse pré-natal, perfil de ansiedade de progenitoras na gestação e de sua progênie ao longo do desenvolvimento

Pós-graduação em Biociências - FCLAS

Repercusões a longo prazo do padrão alimentar ocidental ocorrido durante a gestação, lactação e fase juvenil na susceptibilidade ao desenvolvimento de câncer do cólon em ratos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeitos da restrição de sono em ratas prenhes no desenvolvimento da prole

Na sociedade atual, é comum a diminuição do tempo de sono da população visando cumprir as inúmeras exigências da rotina diária do estilo agitado de vida atual. As consequências da falta de sono são diversas e afetam mais as mulheres que os homens, podendo influenciar diretamente a gravidez. Assim, o objetivo do presente estudo foi avaliar os efeitos da restrição de sono durante o período gestacional no desenvolvimento dos filhotes. Os descendentes foram analisados quanto ao desenvolvimento físico e reflexológico desde o 1º dia de vida pós-natal até a vida adulta reprodutiva. Ao final dos experimentos, os animais foram eutanasiados para coleta de sangue para dosagens de testosterona e progesterona. Os resultados obtidos mostraram que a restrição de sono (RS) durante a prenhez foi prejudicial ao desenvolvimento pós-natal dos descendentes. Houve danos em diversos parâmetros do desenvolvimento físico e sensório-motor, como abertura dos olhos, desdobramento das orelhas e reflexo de sobressalto auditivo. O comportamento sexual dos machos também foi afetado, evidenciado pelo aumento da latência para monta e menor número de montas, caracterizando diminuição da motivação sexual. Isso se deve principalmente ao declínio de progesterona no grupo RS. O desempenho sexual desses animais, no entanto, não foi prejudicado, uma vez que não houve alteração na concentração de testosterona no grupo RS, comparado ao grupo CTRL. Houve também um aumento no interesse sexual nas descendentes fêmeas, possivelmente devido à elevação da testosterona no grupo RS. Esperou-se com este projeto traçar a dimensão dos efeitos da falta de sono durante a gestação sobre o desenvolvimento da prole, procurando conscientizar a mulher da importância do sono para o seu bebê

Diagnóstico de gestação em cadelas: aspectos radiográficos e ultrassonográficos

The modernization of image equipaments associate to the veterinary professionals qualification allowed an advance in diagnosis veterinary medicine, and, consequently, in premature pregnancy diagnosis in bitches. Currently, owners and creators search for information related to pregnancy detection, fetal development and viability and litter size determination. Through diagnosis methods as radiology and ultrasonography, associated to clinics aspects, the diagnosis of early pregnancy become more acurated and precise, allowing more quality in the accompaniment of prenatal of these bitches. Ultrasonographic exams help the accompaniment of these pregnancies through of visuals recourses, which can supply information related to pregnancy detection, embrionary and fetal development, fetal viability e litter size determination through fetals mensurations. The radiographic study has as principal indication the fetal counting, and has been the most acurated for this analyses. It may be done after the da s after the luteini ing hormone surge, when occurs the fetal mineralization. Due to its importance in the present time and in small animals clinic, the objective of this study is to discuss the main radiographics and ultrassonographics aspects of the diagnosis of pregnancy in bitches

Ultrasonografia Doppler na reprodução de pequenos animais

The Doppler ultrasound allows the assessment of blood flow and the differentiate vessels according to each spectral pattern, location and organ that irrigate, helping the examinations and disease diagnosis. The main methods used are Pulsed Doppler, Color Doppler and Power Doppler. In Brazil veterinary medicine, it shows progress since 1990, but its use is limited by the equipment high cost, the need for operator experience and patient cooperation. There are studies in obstetrics and reproduction of dogs and cats, such as evaluation of the testicular vasculature, including the use of contrast material, and attempts to evaluate prostate in healthy dogs or pathological conditions (cancer and testicular torsion , orchitis and benign prostatic hyperplasia; estrous cycle monitoring, evaluation of irrigation in follicles and corpus luteum, uterus and breasts (mammary tumors) in bitches; monitoring pregnancy from the assessment of maternal, fetal and placental hemodynamics, both in dogs as in cats, and aspirations for early detection of pregnancy in dogs. However, new studies are still required, given the lack of these species, especially cats

Avaliação dos custos da assistência de recém-nascidos de mães hipertensas no Hospital das Clínicas - UNESP - Botucatu

The Hypertension Arterial Gestationis is a of largest complications to the pregnant women, a time that is associated with to high risk of morbimortalidade fetal and maternal ;the term If referred the levels pressure equal or above of 140mmhg to the pressure systolic and of 90mmhg to the pressure diastolic (1).Hypertension in pregnancy can be classified into gestational hypertension, chronic hypertension, preeclampsia and eclampsia(3). This study aimed to calculate and analyze the cost of care of newborns of hypertensive mothers hospitalized in rooming, nursery and the neonatal intensive care unit (Neonatal UTI). It’s a study of exploratory, descriptive and quantitative data analysis, in newborns of mothers with hypertension, who underwent prenatal care in HCFMB, from January 1 to 31 in December 2010. The data analysis showed that the cost of care for newborn in rooming was R$ 38.62 for the control group and groups of hypertensive mothers were R$ 19.93 to R$ 37.38. The costs of care to the newborn in the nursery were R$ 1,781.81 for the control group and groups of hypertensive mothers were R$ 680.03 to 7544.10. The costs for the newborn who Neonatal UTI were R$ 7,468.60 for the control group and groups of hypertensive mothers were R$ 5,228.02 to R $ 18,372.75. The total costs of care for newborn in rooming, nursery and Neonatal UTI were R$ 916.15 for the control group, R$ 1,385.98 for the HAC group, R$ 327.23 for the group HAS, R$ 3,896.57 for the group of preeclampsia and R$ 6,326.54 for the group of eclampsia. Considerations It can be concluded that the costs of mothers with preeclampsia and eclampsia were higher, being conditions with increased risk of maternal-fetal morbidity / mortality, requiring care in intensive care unit and longer stay in hospital

Determinação de adenosina deaminase, fator de necrose tumoral-alfa e interleucina-1 em gestantes portadoras de pré-eclâmpsia

Preeclampsia is a specific disorder of pregnancy, characterized by arterial hypertension and proteinuria detected after 20 weeks of gestation. This pathology is associated with hyperuricemia, higher levels of pro-inflammatory cytokines, enhanced leukocyte activation and oxidative stress. Adenosine deaminase (ADA) is an enzyme present in all human tissues and, it is involved with the maturation of the immune system. Although its function is not fully understood, ADA is considered an indicator of cellular inflammation and, its increased serum concentration is observed in inflammatory diseases, such as tuberculosis and rheumatoid arthritis. This study aimed to assess serum ADA levels in preeclamptic patients (PE) compared with normotensive pregnant (NT) and non-pregnant women (NP), and to correlate these values with TNF-α and IL-1β production. Ninety pregnant women were included: 60 were pre-eclamptic and 30 were normotensive matched for gestational age. As control group 20 healthy non-pregnant women matched with pregnant for age were included. Peripheral blood mononuclear cells (PMMC) obtained from the three groups studied were cultured with or without lipopolysaccharide (LPS) for 18h at 37oC, and TNF-α and IL-1β production was assessed in the supernatant of cultures by enzyme immunoassay (ELISA). ADA plasmatic concentration was determined by colorimetric method. The results show that ADA plasma levels were significantly higher in PE group compared with NT and NP groups. A positive correlation between ADA and uric acid levels was detected in preeclamptic women. There was no significant difference in relation to ADA levels when PE patients were classified in early and late-onset PE. The endogenous production of IL-1β and TNF-α by PBMC was significantly higher in PE group than in NT and NP women, showing the activation state of these cells in PE. LPS induced...(Complete abstract click electronic access below)

Polimorfismo do gene IL6 candidato à predisposição à rotura prematura de membranas pré-termo e ao trabalho de parto pré-termo

Introduction: Preterm Labor (PTL) and Preterm Premature Rupture of Membranes (PPROM) cause severe complications for both mother and fetus. Among the risk factors associated with preterm labor and PPROM, genetic predisposition has been gaining importance. However, the association between polymorphic genes and the pathogenesis of PTL and PPROM remains elusive. A better understanding of the genetic mechanisms underlying these adverse pregnancy outcomes may enable the identification of high risk patients and allow new approaches to minimize the deleterious effects of prematurity. Aim: To determine the association between maternal IL-6 polymorphism gene and the occurrence of PTL and PPROM. Patients and Methods: The study included 109 patients with prior history of PL and/or PPROM that delivered prematurely at the Obstetrical Unit Care of Botucatu Medical School, UNESP between 2003 and 2012. The control group consisted of 68 patients that delivered at term, matched to the case group by age, ethnicity, and sex of the newborn. Oral swabs (Cath-AllTM – Epicentre Biotechnologies) were collected for analysis of genetic polymorphisms by PCR. Statistical tests were performed to compare genotype, clinical and socio-demographic data from the groups. A p-value of <0.05 was considered significant. Results: The sociodemographic characteristics in both groups were homogeneously distributed. The frequency of the polymorphic allele C, associated with less production of IL-6, and therefore thought to be protective against PTL and PPROM, was 32,5% in the study group and 30,9% in the control group, without statistically significant differences. Conclusion: Considering the sample size included in this study, the frequency of the mutated allele is similar in pregnant women who delivered at term and gestational complications as PTL and PPROM

Perfil epidemiológico de parturientes submetidas à cesariana na maternidade de um hospital escola de Botucatu-SP

During the pregnancy process, the maternal organism commonly undergoes changes. Such changes follow the normal course of pregnancy; however, some maternal or fetal factors can interfere with pregnancy and adversely affect its outcomes, thus triggering what is referred to as high-risk pregnancy, which is responsible for increasing maternal, fetal or newborns’ morbimortality rates1,2. One of the obstetric alternatives for a safe resolution of this pregnancy type is the caesarean section. Nevertheless, an expressive increase in the number of caesarean sections has been observed worldwide, and they are, many times, unnecessarily indicated8. The world Health Organization (WHO) recommends that the total number of caesarean sections in relation to the total number of deliveries performed at health service should be a maximum of 15% 11, a limit that is easily surpassed in various services. To outline the epidemiological profile of pregnant women submitted to caesarean sections at a reference health care service in the city of Botucatu-SP. This is a cross-sectional, retrospective, exploratory, descriptive, quantitative study. The target population consisted of one hundred pregnant women assisted in this institution, who had been submitted to caesarean sections in 2010 and were randomly selected to compose the sample. In the analyzed period, there were 1,189 deliveries, of which 601 (50.5%) were natural deliveries, 588 (49.4%) were caesarean sections. As regards maternal age, 76% were in the age range of 19 to 36 years. A high percentage of patients (27%) had not completed elementary education and did not have a paid job; 67% were homemakers. Most of the participants were married (56%); 34% of the women were primiparas, but 19% were in their fourth or more pregnancies. Concerning prenatal care, little was found, since many of them had consultations out of the institution which were not recorded... (Complete abstract click electronic access below)

Expressão do mRNA do gene Capn 2 e índice de apoptose de ilhotas pancreáticas de rato tratadas com fibrilas de amilina e com oligômeros de amilina de tamanho intermediário

O depósito de amilina é um achado histopatológico freqüente em pacientes portadores de diabetes melito tipo 2 (DM 2) e parece estar relacionado à disfunção da célula beta pancreática característica desta doença. Apenas as moléculas de amilina que sofrem agregação in vivo, tal como a amilina humana, são citotóxicas para as células beta, enquanto as variedades não agregantes, como a amilina de rato, não apresentam efeito deletério. Com o objetivo de compreender melhor os mecanismos de toxicidade associados à forma agregante da amilina, um projeto em andamento em nosso laboratório estudou, por micro-arranjos de cDNA, o perfil de genes modulados pela amilina humana em ilhotas pancreáticas murídeas, comparando duas situações específicas: ilhotas tratadas com fibrilas de amilina (“amilina madura”) e ilhotas tratadas com oligômeros de tamanho intermediário (“amilina fresca”), já que evidências recentes apontam para um efeito deletério apenas de oligômeros de amilina. As ilhotas foram isoladas a partir de ratos Wistar, mantidas em cultura por 24 horas e a seguir tratadas com 10

Incidência de colonização retovaginal por Streptococcus agalactiae em gestantes e avaliação de cultura de swabs combinados

A identificação de fatores de risco para colonização vaginal materna por Streptococcus agalactiae tem sido objeto de estudo na literatura mundial pois essa colonização frequentemente é assintomática e pode causar bacteremia nos recémnascidos, com significante morbidade e mortalidade, especialmente em prematuros. O objetivo do estudo foi associar a colonização por S. agalactiae com o padrão da microbiota vaginal das gestantes e avaliar a eficácia de swabs combinados na detecção de S. agalactiae. Foram incluídas no estudo 405 gestantes em idade gestacional entre 35 e 37 semanas, atendidas no Pré-Natal do Hospital das Clínicas da Faculdade de Medicina de Botucatu, UNESP. Utilizando-se swabs estéreis foram obtidas amostras da região anorretal, do intróito vaginal e do terço distal da parede vaginal. O material coletado foi cultivado em caldo Todd Hewit suplementado com colistina (10g/mL) e ácido nalidíxico (15g/mL), por 18 a 24 horas à 37oC, em seguida, realizada subcultura em ágar-sangue a 5% sob as mesmas condições. As colônias sugestivas de S. agalactiae foram submetidas a coloração de Gram e ao teste da catalase e ao CAMP test. O padrão de microbiota vaginal foi avaliado empregandose a técnica de coloração de Gram. Os dados sócio-demográficos e obstétricos foram obtidos por formulário próprio. Considerando como variável resposta a colonização materna ou não por S. agalactiae, foi ajustado um modelo de regressão logística adotando o método stepwise, considerando as variáveis explanatórias quantitativas e qualitativas. Para positividade de cultura em swabs combinados e isolados foi empregado o teste de Tukey. colonização materna por S. agalactiae foi de 25,4%. Em relação à microbiota vaginal, as alterações mais freqüentes foram vaginose citolítica (11,3%) seguido de vaginose bacteriana (10,9%), candidíase (8,2%) e Flora II ...(Resumo completo, clicar acesso eletrônico abaixo)