Dephosphorylation of ezrin as an early event in renal microvillar breakdown and anoxic injury.

Disruption of the renal proximal tubule (PT) brush border is a prominent early event during ischemic injury to the kidney. The molecular basis for this event is unknown. Within the brush border, ezrin may normally link the cytoskeleton to the cell plasma membrane. Anoxia causes ezrin to dissociate from the cytoskeleton and also causes many cell proteins to become dephosphorylated in renal PTs. This study examines the hypothesis that ezrin dephosphorylation accompanies and may mediate the anoxic disruption of the rabbit renal PT. During normoxia, 73 +/- 3% of the cytoskeleton-associated (Triton-insoluble) ezrin was phosphorylated, but 88 +/- 6% of dissociated (Triton-soluble) ezrin was dephosphorylated. Phosphorylation was on serine/threonine resides, since ezrin was not detectable by an antibody against phosphotyrosine. After 60 min of anoxia, phosphorylation of total intracellular ezrin significantly decreased from 72 +/- 2% to 21 +/- 9%, and ezrin associated with the cytoskeleton decreased from 91 +/- 2% to 58 +/- 2%. Calyculin A (1 microM), the serine/threonine phosphatase inhibitor, inhibited the dephosphorylation of ezrin during anoxia by 57% and also blocked the dissociation of ezrin from the cytoskeleton by 53%. Our results demonstrate that (i) the association of ezrin with the renal microvillar cytoskeleton is correlated with phosphorylation of ezrin serine/threonine residues and (ii) anoxia may cause disruption of the renal brush border by dephosphorylating ezrin and thereby dissociating the brush border membrane from the cytoskeleton.

La historia de la química: Pequeña guía para navegantes. Parte I: Viejas y nuevas tendencias

Esta guía consta de dos partes. En la primera se describe el desarrollo de la historia de la química como área de investigación. Se analizan tanto los primeros trabajos de los químicos-historiadores de los siglos XVIII como las obras de los autores del siglo XIX como Hermann Kopp. También se estudia la incipiente institucionalización en las primeras décadas del siglo XX, con especial atención a las obras de Hélène Metzger y Aldo Mieli. Finalmente, se discuten las principales tendencias actuales. En un próximo artículo se ofrecerá una lista ordenada de recursos bibliográficos y electrónicos para trabajar en historia de la química.

University of Michigan Football Team, 1917

Back Row: Chester Morrison, ?, Angus Goetz, Harold Cherry, ?, Abraham Cohn, ?, Eddy, Ward Culver, ?

Third Row: ?, Archie Weston, Oscar Cartwright, Skinner, trainer Harold Tuthill

Second Row: ?, J .Orton Goodsell, William Fortune, ?, Alan W. Boyd, Tad Wieman, Clifford Sparks

Front Row: Fred Hendershott, others unidentified

Others on team bur not identified in this picture:Charles Booth, Lee Bonar, William Cruse, Harold Froemke, Thomas Garrett, Lowell Genebach, Joseph Hanzlik, Oscar Lambert, L.O. Lindstom, Harold Rye, Richard F. Weske

1948 University of Michigan Men's Golf Team

Back Row: David Barclay, Roger Kessler, Charles MacCallum, Douglas Beath, Tom Messinger

Front Row: Ed Scahlon, coach Bert Katzenmeyer, ?, Morton Cohn

Employment and income distribution objectives for A.I.D. programs and policies /

Includes bibliographical references.

The Across North America Tracer Experiment (ANATEX) model evaluation study /

Mode of access: Internet.

David Wolfsohn, Herzl's successor,

Mode of access: Internet.

The Voyage of Admiral George Carlton, in search of loyalty. : A poetic epistle.

Page [2] is blank.

Principles of knowledge representation and reasoning : proceedings of the Seventh International Conference (KR2000), Breckenridge, Colorado, April 12-15 2000 /

Includes bibliographical references and index.

The works of Charles Dickens,

Title-pages in red and black.

Reise in Ostafrika in den Jahren 1903-1905, mit Mitteln der Hermann und Elise geb. Heckmann Wentzel-Stiftung ausgeführt. Wissenschaftliche Ergebnisse.

Contributions in German, English, French or Italian.

Combustion on wheels, an informal history of the automobile age ...

At head of title: David L. Cohn.

Philonis Alexandrini Opera qvæ svpersvnt /

Vols. I, IV, V edited by Leopold Cohn, vol. II, III by Paul Wendland, vol. VI by Leopold Cohn and Siegfried Reiter, vol. VII by Hans Leisegang.

Die suffixwandlungen im Vulgärlatein und im vorlitterarischen Französisch nach ihren Spuren im Neufranzösischen /

Includes bibliographical references and index.