CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere

Inheritance of each chromosome depends upon its centromere. A histone H3 variant, centromere protein A (CENP-A), is essential for epigenetically marking centromere location. We find that CENP-A is quantitatively retained at the centromere upon which it is initially assembled. CENP-C binds to CENP-A nucleosomes and is a prime candidate to stabilize centromeric chromatin. Using purified components, we find that CENP-C reshapes the octameric histone core of CENP-A nucleosomes, rigidifies both surface and internal nucleosome structure, and modulates terminal DNA to match the loose wrap that is found on native CENP-A nucleosomes at functional human centromeres. Thus, CENP-C affects nucleosome shape and dynamics in a manner analogous to allosteric regulation of enzymes. CENP-C depletion leads to rapid removal of CENP-A from centromeres, indicating their collaboration in maintaining centromere identity.

National Conference on Less Costly Wastewater Treatment Systems for Small Communities, U.S. Environmental Protection Agency, Date: April 12, 13, and 14, 1977, Location: Reston, Virginia.

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Employment and inflation goals of the Humphrey-Hawkins act and the conduct of monetary policy [microform] : hearings before the Subcommittee on Domestic Monetary Policy of the Committee on Banking, Finance, and Urban Affairs, House of Representatives, Ninety-sixth Congress, first session, March 13 and 14, 1979.

Reuse of record except for individual research requires license from Congressional Information Service, Inc.

Allegations of improper lobbying by Department of Defense personnel of the C-5B and B-1B aircraft and sale to Saudi Arabia of the airborne warning and control system : report together with separate views of the Investigations Subcommittee of the Committee on Armed Services, House of Representatives, Ninety-seventh Congress, second session, December 30, 1982.

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Publications de la Cour Permanente de Justice Internationale. Publications of the Permanent Court of International Justice. Series C, Acts and documents relating to judgements and advisory opinions given by the Court.

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Reorganization plan no. 5 of 1961: National Labor Relations Board. Hearings before the Committee on Government Operations, United States Senate, Eighty-seventh Congress first session, resolution of disapproval, S. Res. 158. July 12, 13, and 14, 1961.

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Diagnostic motor vehicle inspection demonstration projects, program engineering support. Volume 4 - appendix C: survey and evaluation of inspector training programs. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

The cases of polygamy, concubinage, adultery, divorce, &c. seriously and learnedly discussed. : Being a compleat collection of all the remarkable tryals and tracts which have been written on those important subjects. /

Reissued 1736 with title: Select and curious cases of polygamy.