557 resultados para threaded posts


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Disbound Original Held in Oak Street Library Facility.

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verso: Grand Island near Munising, Mich.

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Reports cover the calendar year unless otherwise noted.

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--VI. Insects, pt. II. Hymenoptera continued (Tubilifera and Aculeata), Coleoptera, Strepsiptera, Lepidotera, Diptera, Aphaniptera, Thysanoptera, Hemiptera, Anoplura. By David Sharp. 1901.--VII. Hemichordata, by S.F. Harmer. Ascidians and Amphioxus, by W.A. Herdman. Fishes (exclusive of th systematic account of Teleostei) by T.W. Bridge. Fishes (systematic account of Teleostei) by G.A. Bonlenger. 1904.--VIII. Amphibia and reptiles, by Hans Gadow. 1901.--IX. Birds, by A.H. Evans. 1900--X. Mammalia, by F.E. Beddard. 1902.

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Issued Successively by: Postmaster General; Dept. of Posts and Telegrpahs

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Thesis (Ph.D.)--University of Washington, 2016-06

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In recent years an increasing number of miniproteins containing an amide-cyclized backbone have been discovered. The cyclotide family is the largest group of such proteins and is characterized by a circular protein backbone and six conserved cysteine residues linked by disulfide bonds in a tight core of the molecule. These form a cystine knot in which an embedded ring formed by two of the disulfide bonds and the connecting backbone segment is threaded by a third disulfide bond. In the current study we have undertaken high resolution structural analysis of two prototypic cyclotides, kalata B1 and cycloviolacin O1, to define the role of the conserved residues in the sequence. We provide the first comprehensive analysis of the topological features in this unique family of proteins, namely rings (a circular backbone), twists (a cis-peptide bond in the Mobius cyclotides) and knots (a knotted arrangement of the disulfide bonds).

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Life cycle models have become important in explaining the changing size structure of firms based on the carrying capacity of regions or industries. In particular, the population ecology model predicts stages of growth, maturity and eventually decline in the number of firms in an industry. There has been criticism of such models because of their focus on external variables as pre-determinants of the potential for enterprise development. This paper attempts to reconcile the external focus of the population ecology model with relevant internal management factors in enterprise development. A survey was conducted of Australian services exporters, and the results not only confirm the existence of four separate life cycle stages in the population ecology model, but also identify the external and internal variables that are strategically relevant at each of the stages. The findings provide potentially useful information in a range of contexts including the design of small business assistance as well a providing “guide posts” to entrepreneurs engaged in enterprise development.

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sThe structure of a two-chain peptide formed by the treatment of the potent antimicrobial peptide microcin J25 (MccJ25) with thermolysin has been characterized by NMR spectroscopy and mass spectrometry. The native peptide is 21 amino acids in size and has the remarkable structural feature of a ring formed by linkage of the side chain of Glu8 to the N-terminus that is threaded by the C-terminal tail of the peptide. Thermolysin cleaves the peptide at the Phe10-Val11 amide bond, but the threading of the C-terminus through the N-terminal ring is so tight that the resultant two chains remain associated both in the solution and in the gas phases. The three-dimensional structure of the thermolysin-cleaved peptide derived using NMR spectroscopy and simulated annealing calculations has a well-defined core that comprises the N-terminal ring and the threading C-terminal tail. In contrast to the well-defined core, the newly formed termini at residues Phe10 and Val11 are disordered in solution. The C-terminal tail is associated to the ring both by hydrogen bonds stabilizing a short beta-sheet and by hydrophobic interactions. Moreover, unthreading of the tail through the ring is prevented by the bulky side chains of Phe19 and Tyr20, which flank the octapeptide ring. This noncovalent two-peptide complex that has a remarkable stability in solution and in highly denaturing conditions and that survives in the gas phase is the first example of such a two-chain peptide lacking disulfide or interchain covalent bonds.

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Cyclotides are peptides from plants of the Rubiaceae and Violaceae families that have the unusual characteristic of a macrocylic backbone. They are further characterized by their incorporation of a cystine knot in which two disulfides, along with the intervening backbone residues, form a ring through which a third disulfide is threaded. The cyclotides have been found in every Violaceae species screened to date but are apparently present in only a few Rubiaceae species. The selective distribution reported so far raises questions about the evolution of the cyclotides within the plant kingdom. In this study, we use a combined bioinformatics and expression analysis approach to elucidate the evolution and distribution of the cyclotides in the plant kingdom and report the discovery of related sequences widespread in the Poaceae family, including crop plants such as rice ( Oryza sativa), maize ( Zea mays), and wheat ( Triticum aestivum), which carry considerable economic and social importance. The presence of cyclotide-like sequences within these plants suggests that the cyclotides may be derived from an ancestral gene of great antiquity. Quantitative RT-PCR was used to show that two of the discovered cyclotide-like genes from rice and barley ( Hordeum vulgare) have tissue-specific expression patterns.

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Retrieving large amounts of information over wide area networks, including the Internet, is problematic due to issues arising from latency of response, lack of direct memory access to data serving resources, and fault tolerance. This paper describes a design pattern for solving the issues of handling results from queries that return large amounts of data. Typically these queries would be made by a client process across a wide area network (or Internet), with one or more middle-tiers, to a relational database residing on a remote server. The solution involves implementing a combination of data retrieval strategies, including the use of iterators for traversing data sets and providing an appropriate level of abstraction to the client, double-buffering of data subsets, multi-threaded data retrieval, and query slicing. This design has recently been implemented and incorporated into the framework of a commercial software product developed at Oracle Corporation.

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O presente estudo confirmou a existência da substituição do trabalho vivo pelo trabalho morto, descrita por Karl Marx e outros importantes estudiosos, pela EC ABC, empresa do setor eletroeletrônico brasileiro (segmento das empresas industriais estabelecidas no Brasil, responsáveis pela produção de equipamentos que atendem a linha marrom que congrega produtos como, Televisores, Monitores de Vídeo, Áudio, reprodutores e/ou gravadores de Disco de Vídeo Versátil - DVD e Disco Compacto - CD), inicialmente no seu Departamento de Serviços Nacional e em seguida, nos Postos de Serviços Credenciados da sua Rede de Serviços Nacional, em função da informatização e mudança do modelo de gestão do conhecimento o que ocasionou a descapitalização intelectual nos Postos de Serviços Credenciados e a perda de qualidade nos serviços oferecidos pelos PSC aos clientes e revendedores. Adotou-se a forma de pesquisa exploratória deste fenômeno, atualmente ainda pouco examinado, em 120 PSC no Brasil.(AU)

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O trabalho é resultado de pesquisa realizada sobre a gestão da Comunicação em processos de crise organizacional. Busca-se compreender como se processa, na atualidade, o fluxo informacional nesses momentos, seja pela mídia tradicional,seja pelas redes sociais online, e, dessa forma, trazer contribuições para a atuação das organizações sob uma perspectiva ética. Em termos metodológicos, a pesquisa apoia-se no estudo de caso da gestão da Comunicação da crise do Playcenter, ocorrida em abril de 2011. Utiliza-se de revisão bibliográfica, análises de matérias jornalísticas, posts do Twitter, além de entrevistas semiestruturadas. Os resultados revelam que a imagem não é uma questão de emissão, mas de recepção, resultado de percepções diversas e que esses fenômenos, na atualidade, têm sido potencializados pela rápida propagação dos fatos facilitada pela internet. Entretanto, ainda há um forte papel das mídias tradicionais nesses episódios, forte convergência entre as mídias tradicionais e as novas mídias, bem como convergência entre os cidadãos na propagação dos fatos.