504 resultados para pyroligneous liquor


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Attributed to Lucius M. Sargent. Cf. NUC pre-1956 imprints.

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Formerly Internal Revenue Service publication 455.

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Mode of access: Internet.

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pt.1. House Committee on Agriculture.--pt.2. House Committee on Appropriations.--pt.3. House Committee on Accounts, House Committee on Alcoholic Liquor Traffic, House Committee on Banking and Currency, House Committee on the Census, House Committee on Claims [and] House Committee on Coinage, Weights, and Measures.

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Introductory note.--The defence of the realm acts, as passed, with notes.--The Defence of the realm regulations ... as provided by order in Council, with notes.--The Defence of the realm (liquor control) regulations, 1915, and orders in Council applying the same, as passed with notes.--Analytical index to acts, regulations, and introductory and other notes.

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"An encyclopedia of facts and figures dealing with the liquor traffic and the temperance reform"

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"Historical sketch of United States Brewers' Association": year book for 1909, p. [11]-22.

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Shipping list no. 86-418-P.

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Includes bibliographical references.

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A denitrifying microbial consortium was enriched in an anoxically operated, methanol-fed sequencing batch reactor (SBR) fed with a mineral salts medium containing methanol as the sole carbon source and nitrate as the electron acceptor. The SBR was inoculated with sludge from a biological nutrient removal activated sludge plant exhibiting good denitrification. The SBR denitrification rate improved from less than 0.02 mg of NO3-.N mg of mixed-liquor volatile suspended solids (MLVSS)(-1) h(-1) to a steady-state value of 0.06 mg of NO3-.N mg of MLVSS-1 h(-1) over a 7-month operational period. At this time, the enriched microbial community was subjected to stable-isotope probing (SIP) with [C-13] methanol to biomark the DNA of the denitrifiers. The extracted [C-13]DNA and [C-12]DNA from the SIP experiment were separately subjected to full-cycle rRNA analysis. The dominant 16S rRNA gene phylotype (group A clones) in the [C-13]DNA clone library was closely related to those of the obligate methylotrophs Methylobacillus and Methylophilus in the order Methylophilales of the Betaproteobacteria (96 to 97% sequence identities), while the most abundant clone groups in the [C-12]DNA clone library mostly belonged to the family Saprospiraceae in the Bacteroidetes phylum. Oligonucleotide probes for use in fluorescence in situ hybridization (FISH) were designed to specifically target the group A clones and Methylophilales (probes DEN67 and MET1216, respectively) and the Saprospiraceae clones (probe SAP553). Application of these probes to the SBR biomass over the enrichment period demonstrated a strong correlation between the level of SBR denitrification and relative abundance of DEN67-targeted bacteria in the SBR community. By contrast, there was no correlation between the denitrification rate and the relative abundances of the well-known denitrifying genera Hyphomicrobium and Paracoccus or the Saprospiraceae clones visualized by FISH in the SBR biomass. FISH combined with microautoradiography independently confirmed that the DEN67-targeted cells were the dominant bacterial group capable of anoxic [C-14] methanol uptake in the enriched biomass. The well-known denitrification lag period in the methanol-fed SBR was shown to coincide with a lag phase in growth of the DEN67-targeted denitrifying population. We conclude that Methylophilales bacteria are the dominant denitrifiers in our SBR system and likely are important denitrifiers in full-scale methanol-fed denitrifying sludges.