Values of 13,14-Dihydro-15-Keto-PGF(2 alpha), Progesterone and Oestradiol After Injection of Prostaglandin at Different Periods of Buffalo Puerperium

Knowledge of the effectiveness of prostaglandins in uterine involution process led to the development of protocols with its analogues in postpartum period. However, this hormone mechanism of action is not yet fully elucidated. Thus, the objective of this study was to verify if chloprostenol administration, at early or intermediary puerperium, can induce changes on progesterone, PGFM and oestradiol plasma concentrations. 30 Murrah postpartum buffaloes were randomly divided into three groups: CONT (saline, n = 10); CLO2 (chloprostenol at days 2 and 5 postpartum, n = 10) and; CLO15 (chloprostenol at days 15 and 20 postpartum, n = 10). Blood samples were collected from jugular vein to measure progesterone, PGFM and oestradiol plasma concentrations at days 2, 7, 14, 21 and 28 postpartum. CLO2 group presented lower progesterone and PGFM plasma concentrations in relation to CONT and CLO15 groups (0.23 +/- 0.00 and 0.32 +/- 0.11, 0.19 +/- 0.00 and 0.23 +/- 0.11, 0.23 +/- 0.00 and 0.30 +/- 0.19, for groups CONT, CLO2 and CLO15, respectively; P < 0.05). There was no significant difference in oestradiol plasma concentration between experimental groups (P > 0.05). Prostaglandin synthetic analogue administration induced hormonal changes in postpartum buffaloes, which can partially explain its positive effect under reproductive function of this specie.

Comparison of Botu-bov and Tris as Freezing Extenders of Buffalo Sperm Recovered from Epididymal Cauda

Valuable genetic material can be preserved by the cryopreservation of epididymal sperm. This study evaluated the viability of pre-freezing and post-thawed sperm samples recovered from the epididymal cauda of buffaloes. Epididymides from eight Murrah buffaloes with 18 months of age were used. Semen samples were diluted in two different freezing extenders: Botu-bov (BB) and Tris (TRIS). Immediately after slaughter, both testicles from each animal were collected and transported at 4 degrees C for six hours interval. In laboratory, the removed epididymides were flushed to obtain sperm and the fractions were diluted in both freezing extenders (BB and TRIS). Semen doses were analyzed before and after frozen at -196 degrees C. BB and TRIS pre-freezing results were 38.54 +/- 22.33%(b) and 14.17 +/- 12.78%(a) for total motility (TM), 25.00 +/- 16.12(a) and 9.44 +/- 9.11(a) for progressive motility (PM), 7.21 +/- 0.98(a) and 5.09 +/- 2.65(a) for percentage of rapid cells (RAP), 91.08 +/- 12.53(b) and 63.33 +/- 31.47(a) for velocity of trajectory (VAP), 73.54 +/- 20.17(b) and 49.50 +/- 9.11(a) for linear progressive velocity (VSL), 172.21 +/- 24.55(a) and 116.94 +/- 59.48(a) for curvilinear velocity (VCL), respectively (P < 0.05). BB and TRIS post-thawing results were 42.25 +/- 21.50(b) and 17.62 +/- 19.46(a) for TM, 27.25 +/- 24.86(a) and 18.00 +/- 13.68(a) for PM, 7.35 +/- 0.98(a) and 6.26 +/- 1.13(a) for RAP, 91.42 +/- 16.86(a) and 75.96 +/- 13.17(a) for VAP, 67.96 +/- 12.13(a) and 60.04 +/- 10.42(a) for VSL, 177.54 +/- 23.53(b) and 141.29 +/- 24.97a for VCL, respectively (P < 0.05). The sperm recovered from the epididymal cauda, after 6 h storage of epididymides at 5 degrees C ensures sperm preservation demonstrating that the diluent Botu-bov had higher total motility both pre-and post-freezing when compared with TRIS. Additionally, the sperm frozen with the diluent Botu-bov showed higher values of VSL at post-thawing. These findings may reflect in improvement of conception rates.

Adding Motility Stimulants to Improve Freezing of Buffalo Sperm Recovered from Epididymal Cauda

The cryopreservation of epididymal sperm is important to preserve genetic material from valuable buffalo bulls. This study evaluated the viability of post-thawed sperm samples recovered from the epididymal cauda adding motility inductors. For that, were used epididymides from eight Murrah buffaloes with 18 months of age. Semen samples were submitted to three different conditions: (CT - control) without adding medium, (SPERM) adding Sperm Talp medium, and (FERT) adding Fert Talp medium. Immediately after slaughter, both testicles from each animal were collected and transported at 4 degrees C at maximum six hours interval. In laboratory, the removed epididymides was flushed to obtain sperm and diluted in the freezing extender. Each buffalo sperm were divided and fractions were submitted to all conditions (CT, SPERM and FERT). Semen doses were frozen at -196 degrees C. CT, SPERM and FERT post-thawing results were 13.63 +/- 8.91, 38.77 +/- 8.91 and 42.83 +/- 8.91 for total motility, 7.30 +/- 8.74, 24.87 +/- 8.74 and 29.70 +/- 8.74 for progressive motility, 6.04 +/- 0.92, 6.74 +/- 0.92 and 6.93 +/- 0.92 for percentage of rapid cells (P < 0.05). In conclusion, diluted semen supplementation with Sperm or Fert talp increases the motility of cauda epididymal sperm of buffalo bulls.

Uterine Involution, Fluid Accumulation and Ovarian Activity after Injection of Prostaglandin at Different Periods of Buffalo Puerperium

This study aimed to evaluate the effect of chloprostenol administration, at early or intermediary puerperium, under uterine involution, intrauterine fluid accumulation and ovarian activity return. 30 Murrah postpartum buffaloes were randomly divided into three groups: CONT (saline, n = 10); CLO2 (chloprostenol at days 2 and 5 postpartum, n = 10) and; CLO15 (chloprostenol at days 15 and 20 postpartum, n = 10). Gynecological exams were performed at days 2, 7, 14, 21 and 28 postpartum, when uterine involution degree (1 to 3 scale, by transrectal palpation), intrauterine fluid accumulation (0 to 3 scale, by ultrasound exam) and ovarian activity (B-mode ultrasound exam) were evaluated. CLO2 group presented higher uterine involution (2.00 +/- 0.23, 1.66 +/- 0.23, 1.58 +/- 0.23 for groups CLO2, CONT and CLO15, respectively) and faster ovarian activity return in relation to groups CONT and CLO15 (P < 0.05). Groups CLO2 and CLO15 showed lower intrauterine fluid accumulation compared to CONT group (2.04 +/- 0.20, 1.58 +/- 0.20, 1.92 +/- 0.20 for groups CONT, CLO2 and CLO15, respectively; P < 0.05). Prostaglandin analogue administration in postpartum buffalo benefited uterine involution, lochia expulsion and ovarian activity return, improving reproductive efficiency in this specie.

Tuberculosis prevalence and risk factors for water buffalo in Para, Brazil

The prevalence of and possible risk factors for tuberculosis were studied in water buffalo from Para, Brazil. In this study, 3,917 pregnant and nonpregnant female Murrah and Mediterranean buffaloes were studied; 2,089 originated from Marajo Island, and 1,108 were from the mainland. The comparative cervical tuberculin test was used as a diagnostic test for tuberculosis in these animals. The prevalence of positive buffaloes was 3.5 % (100/2,809) on Marajo Island and 7.2% (80/1,108) on the mainland. The municipalities with the highest tuberculosis prevalence rates in animals were Ipixuna do Para (10.1 %), Marapanim (9.8 %), Chaves (9.4 %), Paragominas (8.6 %), and Cachoeira do Arari (6.7 %). The tuberculosis prevalence was not significantly different between the Murrah (4.3 %) and Mediterranean (4.8 %) breeds or between pregnant (5%) and nonpregnant (4.3%) buffaloes. Tuberculosis was detected in water buffaloes from Para, Brazil; the mainland buffalo exhibited the highest tuberculosis prevalence. These results indicate that this disease is dangerous to public health and buffalo farming in Para.

Detection of Shiga toxigenic (STEC) and enteropathogenic (EPEC) Escherichia coli in dairy buffalo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Características qualitativas e quantitativas do músculo Longissimus de bubalinos e bovinos terminados em confinamento

Pós-graduação em Zootecnia - FCAV

Análise da heterocedasticidade em bubalinos utilizando informações genômicas

Pós-graduação em Genética e Melhoramento Animal - FCAV

Ultra-som para predição da composição e características de carcaça em bubalinos

Este trabalho teve como objetivo avaliar características de carcaça por ultra-som de 28 bubalinos jovens Mediterrâneo terminados em confinamento e abatidos aos 450, 480, 510 e 540 kg de peso vivo (PV). Foi utilizado um equipamento de ultra-som Piemedical Scanner 200 Vet, com transdutor linear de 178 mm e 3,5 MHz, a cada intervalo de aproximadamente 28 dias, para obter a área do longíssímus dorsí (ALOU), espessura de gordura subcutânea (EGSU) entre a 12ª e 13ª costelas e a espessura de gordura na picanha (EGP8U), sob o terço superior do músculo bíceps femorís. Após atingirem os pesos de abate pré-estabelecidos, os animais foram abatidos e obteve-se o peso de carcaça quente (PCQ) e o rendimento de carcaça (RC). Após 24 horas de resfriamento, as carcaças foram seccionadas entre a 12a e 13a costelas e obtidas a área do longíssímus dorsí (ALOC), a espessura de gordura (EGSC) e a espessura de gordura sob o bíceps femorís (EGP8C) na carcaça. As correlações entre as medidas por ultra-som e na carcaça foram de 0,96 entre ALOU e ALOC, de 0,99 entre EGSU e EGSC e de 0,91 entre EGP8U e EGP8C. Equações de regressão utilizando o peso vivo (PV), ALOU, EGSU e EGP8U explicaram 95% da variação do PCQ quando a medida foi realizada imediatamente antes do abate. As equações para estimar o RC utilizando as mesmas características explicaram cerca de 32% da variação quando a medida foi realizada imediatamente antes do abate. O peso da porção comestível do corte traseiro a partir de medidas efetuadas por ultra-som e na carcaça é predito com maior magnitude que a percentagem da porção comestível. Os resultados indicam que as equações para as medidas ultra-sônicas apresentaram boa acurácia e podem ser utilizadas para estimar diferenças entre grupos de animais, mas há a necessidade de mais estudos envolvendo maior número de animais e de outros grupos genéticos de bubalinos.

Water buffalo genome characterization by the Illumina BovineHD BeadChip

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Random regression models using different functions to model milk flow in dairy cows

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção de leite, duração da lactação e intervalo de partos em búfalas mestiças Murrah

This study aimed to evaluate the potential for milk production (MP), lactation length (LL) and calving interval (CI), analyze the environmental component affecting these traits, and to estimate the heritability and repeatability for milk production in crossbreds of Murrah buffalo cows in the state of Alagoas, Brazil. Data was composed of 487 observations of MP from 136 lactations recorded between the years of 2000 and 2010. In the analysis of variance for PL, the fixed effects were season (1- October to March, 2 -April to September) and year of the beginning of lactation, calving order and the LL (covariate). For the analysis of LL only the fixed effect of year of the beginning of lactation was included, and finally for the CI analysis, year of the beginning of lactation and calving order. The estimates of covariance were obtained using unicharacteristic analysis by Bayesian inference method, applyingan animal model, through Gibbs sampling. The additive genetic, permanent environment and residual effects were included as random effects in the model. The averages (sd) of MP, LL and CI were 2,218.03 kg (408.18), 282.59 days (39.48) e 422.49 days (91.05), respectively. All the effects included in the models were important (P<0.01). The estimates of heritability and repeatability for PL were 0.29 and 0.69, respectively. The results suggest that there is a moderate genetic variability among individuals for PL, indicating the possibility to obtain gain using selection.

Monitoramento da Qualidade do leite: Procedimentos Diagnósticos

Means for milk, not otherwise specified, the product from complete and uninterrupted milking , in a hygienic, healthy cows, well fed and rested. The milk of other animals must be called according to origin of species. One of the main characteristics that defined the milk is the set of their sensory characteristics, and the main flavor. The control of milk quality in Brazil has been an important factor for the consolidation of the entire production chain, passing necessarily by the dairy industry. The production of milk with good quality guarantees, of course, food safety for consumers. The measures to obtain milk begin on the property, in carrying out correct procedures for milking, storage and transportation of the product also in the dairy. Later, in the laboratory evaluation of physicochemical properties, research fraud, and microbiological examinations. Finally, measures of health education are need for producers to become aware of the economic benefits by obtaining a better quality milk. The objective of this review is to emphasize the importance of using diagnostic procedures methods for the control of mastitis and consequently obtain better Milk quality

Estudo da interação genótipo ambiente em búfalos leiteiros do Brasil e da Colômbia

Pós-graduação em Zootecnia - FCAV

Práticas higiênico-sanitárias na ordenha de vacas e percepção de risco de produtores rurais no Estado de São Paulo

Pós-graduação em Ciência Animal - FMVA