Microbial−mammalian cometabolites dominate the age-associated urinary metabolic phenotype in Taiwanese and American populations

Understanding the metabolic processes associated with aging is key to developing effective management and treatment strategies for age-related diseases. We investigated the metabolic profiles associated with age in a Taiwanese and an American population. 1H NMR spectral profiles were generated for urine specimens collected from the Taiwanese Social Environment and Biomarkers of Aging Study (SEBAS; n = 857; age 54–91 years) and the Mid-Life in the USA study (MIDUS II; n = 1148; age 35–86 years). Multivariate and univariate linear projection methods revealed some common age-related characteristics in urinary metabolite profiles in the American and Taiwanese populations, as well as some distinctive features. In both cases, two metabolites—4-cresyl sulfate (4CS) and phenylacetylglutamine (PAG)—were positively associated with age. In addition, creatine and β-hydroxy-β-methylbutyrate (HMB) were negatively correlated with age in both populations (p < 4 × 10–6). These age-associated gradients in creatine and HMB reflect decreasing muscle mass with age. The systematic increase in PAG and 4CS was confirmed using ultraperformance liquid chromatography–mass spectrometry (UPLC–MS). Both are products of concerted microbial–mammalian host cometabolism and indicate an age-related association with the balance of host–microbiome metabolism.

Analyses of single nucleotide polymorphisms in selected nutrient-sensitive genes in weight-regain prevention: the DIOGENES study

BACKGROUND: Differences in the interindividual response to dietary intervention could be modified by genetic variation in nutrient-sensitive genes. OBJECTIVE: This study examined single nucleotide polymorphisms (SNPs) in presumed nutrient-sensitive candidate genes for obesity and obesity-related diseases for main and dietary interaction effects on weight, waist circumference, and fat mass regain over 6 mo. DESIGN: In total, 742 participants who had lost ≥ 8% of their initial body weight were randomly assigned to follow 1 of 5 different ad libitum diets with different glycemic indexes and contents of dietary protein. The SNP main and SNP-diet interaction effects were analyzed by using linear regression models, corrected for multiple testing by using Bonferroni correction and evaluated by using quantile-quantile (Q-Q) plots. RESULTS: After correction for multiple testing, none of the SNPs were significantly associated with weight, waist circumference, or fat mass regain. Q-Q plots showed that ALOX5AP rs4769873 showed a higher observed than predicted P value for the association with less waist circumference regain over 6 mo (-3.1 cm/allele; 95% CI: -4.6, -1.6; P/Bonferroni-corrected P = 0.000039/0.076), independently of diet. Additional associations were identified by using Q-Q plots for SNPs in ALOX5AP, TNF, and KCNJ11 for main effects; in LPL and TUB for glycemic index interaction effects on waist circumference regain; in GHRL, CCK, MLXIPL, and LEPR on weight; in PPARC1A, PCK2, ALOX5AP, PYY, and ADRB3 on waist circumference; and in PPARD, FABP1, PLAUR, and LPIN1 on fat mass regain for dietary protein interaction. CONCLUSION: The observed effects of SNP-diet interactions on weight, waist, and fat mass regain suggest that genetic variation in nutrient-sensitive genes can modify the response to diet. This trial was registered at clinicaltrials.gov as NCT00390637.

Multilevel research in the field of organizational behavior: an empirical look at 10 years of theory and research

During the last 30 years, significant debate has taken place regarding multilevel research. However, the extent to which multilevel research is overtly practiced remains to be examined. This article analyzes 10 years of organizational research within a multilevel framework (from 2001 to 2011). The goals of this article are (a) to understand what has been done, during this decade, in the field of organizational multilevel research and (b) to suggest new arenas of research for the next decade. A total of 132 articles were selected for analysis through ISI Web of Knowledge. Through a broad-based literature review, results suggest that there is equilibrium between the amount of empirical and conceptual papers regarding multilevel research, with most studies addressing the cross-level dynamics between teams and individuals. In addition, this study also found that the time still has little presence in organizational multilevel research. Implications, limitations, and future directions are addressed in the end. Organizations are made of interacting layers. That is, between layers (such as divisions, departments, teams, and individuals) there is often some degree of interdependence that leads to bottom-up and top-down influence mechanisms. Teams and organizations are contexts for the development of individual cognitions, attitudes, and behaviors (top-down effects; Kozlowski & Klein, 2000). Conversely, individual cognitions, attitudes, and behaviors can also influence the functioning and outcomes of teams and organizations (bottom-up effects; Arrow, McGrath, & Berdahl, 2000). One example is when the rewards system of one organization may influence employees’ intention to quit and the existence or absence of extra role behaviors. At the same time, many studies have showed the importance of bottom-up emergent processes that yield higher level phenomena (Bashshur, Hernández, & González-Romá, 2011; Katz-Navon & Erez, 2005; Marques-Quinteiro, Curral, Passos, & Lewis, in press). For example, the affectivity of individual employees may influence their team’s interactions and outcomes (Costa, Passos, & Bakker, 2012). Several authors agree that organizations must be understood as multilevel systems, meaning that adopting a multilevel perspective is fundamental to understand real-world phenomena (Kozlowski & Klein, 2000). However, whether this agreement is reflected in practicing multilevel research seems to be less clear. In fact, how much is known about the quantity and quality of multilevel research done in the last decade? The aim of this study is to compare what has been proposed theoretically, concerning the importance of multilevel research, with what has really been empirically studied and published. First, this article outlines a review of the multilevel theory, followed by what has been theoretically “put forward” by researchers. Second, this article presents what has really been “practiced” based on the results of a review of multilevel studies published from 2001 to 2011 in business and management journals. Finally, some barriers and challenges to true multilevel research are suggested. This study contributes to multilevel research as it describes the last 10 years of research. It quantitatively depicts the type of articles being written, and where we can find the majority of the publications on empirical and conceptual work related to multilevel thinking.

Can perceptual differences account for managerial success?: the case of Japanese aid workers

Purpose – There is a wealth of studies which suggest that managers' positive perceptions/expectations can considerably influence the organisational performance; unfortunately, little empirical evidence has been obtained from development studies. This research aims to focus on the perceptual and behavioural trait differences of successful and unsuccessful aid workers, and their relationship with organisational performance. Design/methodology/approach – Through web-based survey, 244 valid responses were obtained from the Japan International Cooperation Agency (JICA)-aid managers worldwide. Five perception related factors were extracted and used for cluster analysis to group the respondents. Each cluster's perception/behaviour-related factors and organisational performance variables were compared by ANOVA. Findings – It was discovered that Japanese's positive perception/expectation about work and their local colleagues was related to higher organisational performance, and conversely, the negative perception on their part was generally associated with negative behaviour and lower organisational performance. Moreover, in a development context, lower work-related stress and feelings of resignation toward work were strongly associated with the acceptability of cross-cultural work environment. Practical implications – The differences in perceptual tendencies suggest that cautious consideration is advised since these findings may mainly apply to Japanese aid managers. However, as human nature is universal, positive perception and behaviour would bring out positive output in most organisations. Originality/value – This study extended the contextualised “Pygmalion effect” and has clarified the influence of perception/expectation on counter-part behaviour and organisational performance in development aid context, where people-related issues have often been ignored. This first-time research provides imperial data on the significant role of positive perception on the incumbent role holder.

Building employee relationships through corporate social responsibility: the moderating role of social cynicism and reward for application

We explore the role of deeply held beliefs, known as social axioms, in the context of employee–organization relationships. Specifically, we examine how the beliefs identified as social cynicism and reward for application moderate the relationship between employees’ work-related experiences, perceptions of CSR, attitudes, and behavioral intentions toward their firm. Utilizing a sample of 130 retail employees, we find that CSR affects more positively employees low on social cynicism and reduces distrust more so than with cynical employees. Employees exhibiting strong reward for application are less positively affected by CSR, whereas their experiences of other work-related factors are more likely to reduce distrust. Our findings suggest the need for a differentiated view of CSR in the context of employee studies and offer suggestions for future research and management practice.

Political culture and corporate homicide liability in the UK and Europe

This chapter outlines recent developments in the emergence within Europe of systems of criminal law designed to hold corporate bodies liable where they cause the deaths of workers or members of the public. These changes point to the emergence of a new, more punitive, legal culture in relation to corporate crime. At the same time, however, there is evidence to suggest that this punitive culture is not uniform; different national jurisdictions reflect it to differing degrees. The chapter explores the degree to which the UK’s willingness to criminalise work-related deaths is mirrored elsewhere in Europe, and identifies some factors that might account for variations in this regard. In particular, attention is paid to the influence that social and political culture have on practices in this area. It is written as part of a research handbook on corporate crime in Europe, so has an eye on a more generalist audience in some regards.

Sensory and consumer science methods used with older adults: a review of current methods and recommendations for the future

Capturing the sensory perception and preferences of older adults, whether healthy or with particular disease states, poses major methodological challenges for the sensory community. Currently a vastly under researched area, it is at the same time a vital area of research as alterations in sensory perception can affect daily dietary food choices, intake, health and wellbeing. Tailored sensory methods are needed that take into account the challenges of working with such populations including poor access leading to low patient numbers (study power), cognitive abilities, use of medications, clinical treatments and context (hospitals and care homes). The objective of this paper was to review current analytical and affective sensory methodologies used with different cohorts of healthy and frail older adults, with focus on food preference and liking. We particularly drew attention to studies concerning general ageing as well as to those considering age-related diseases that have an emphasis on malnutrition and weight loss. Pubmed and Web of Science databases were searched to 2014 for relevant articles in English. From this search 75 papers concerning sensory acuity, 41 regarding perceived intensity and 73 relating to hedonic measures were reviewed. Simpler testing methods, such as directional forced choice tests and paired preference tests need to be further explored to determine whether they lead to more reliable results and better inter-cohort comparisons. Finally, sensory quality and related quality of life for older adults suffering from dementia must be included and not ignored in our future actions.

Argininosuccinate Synthetase Is a Functional Target for a Snake Venom Anti-hypertensive Peptide ROLE IN ARGININE AND NITRIC OXIDE PRODUCTION

Bj-BPP-10c is a bioactive proline-rich decapeptide, part of the C-type natriuretic peptide precursor, expressed in the brain and in the venom gland of Bothrops jararaca. We recently showed that Bj-BPP-10c displays a strong, sustained anti-hypertensive effect in spontaneous hypertensive rats (SHR), without causing any effect in normotensive rats, by a pharmacological effect independent of angiotensin-converting enzyme inhibition. Therefore, we hypothesized that another mechanism should be involved in the peptide activity. Here we used affinity chromatography to search for kidney cytosolic proteins with affinity for Bj-BPP-10c and demonstrate that argininosuccinate synthetase (AsS) is the major protein binding to the peptide. More importantly, this interaction activates the catalytic activity of AsS in a dose-dependent manner. AsS is recognized as an important player of the citrulline-NO cycle that represents a potential limiting step in NO synthesis. Accordingly, the functional interaction of Bj-BPP-10c and AsS was evidenced by the following effects promoted by the peptide: (i) increase of NO metabolite production in human umbilical vein endothelial cell culture and of arginine in human embryonic kidney cells and (ii) increase of arginine plasma concentration in SHR. Moreover, alpha-methyl-DL-aspartic acid, a specific AsS inhibitor, significantly reduced the anti-hypertensive activity of Bj-BPP-10c in SHR. Taken together, these results suggest that AsS plays a role in the anti-hypertensive action of Bj-BPP-10c. Therefore, we propose the activation of AsS as a new mechanism for the anti-hypertensive effect of Bj-BPP-10c in SHR and AsS as a novel target for the therapy of hypertension-related diseases.

Occupational airborne contamination in south Brazil: 1. Oxidative stress detected in the blood of coal miners

Reactive oxygen species and nitrogen species have been implicated in the pathogenesis of coal dust-induced toxicity. The present study investigated several oxidative stress biomarkers (Contents of lipoperoxidation = TBARS, reduced = GSH, oxidized = GSSG and total glutathione = TG, alpha-tocopherol, and the activities of glutathione S-transferase = GST, glutathione reductase = GR, glutathione peroxidase = GPx, catalase = CAT and superoxide dismutase = SOD), in the blood of three different groups (n = 20 each) exposed to airborne contamination associated with coal mining activities: underground workers directly exposed, surface workers indirectly exposed, residents indirectly exposed (subjects living near the mines), and controls (non-exposed subjects). Plasma TBARS were increased and whole blood TG and GSH levels were decreased in all groups compared to controls. Plasma alpha-tocopherol contents showed approximately half the values in underground workers compared to controls. GST activity was induced in workers and also in residents at the vicinity of the mining plant, whilst CAT activity was induced only in mine workers. SOD activity was decreased in all groups examined, while GPx activity showed decreased values only in underground miners, and GR did not show any differences among the groups. The results showed that subjects directly and indirectly exposed to coal dusts face an oxidative stress condition. They also indicate that people living in the vicinity of the mine plant are in health risk regarding coal mining-related diseases.

Faktorer som påverkar sjuksköterskans arbetsrelaterade stress samt konsekvenserna av detta : En litteraturstudie

Syfte: Syftet med litteraturstudien var att undersöka vilka faktorer som påverkar sjuksköterskans arbetsrelaterade stress samt att ta reda på vilka konsekvenser detta kan leda till. Metod: Examensarbetet utfördes som en litteraturstudie med 14 vetenskapliga artiklar. Datainsamling gjordes i databaserna PubMed och CINAHL. Huvudresultat: De faktorer som tydligast framkom genom artiklarnas resultat handlade om sjuksköterskornas psykiska och fysiska arbetsmiljö, vilket innefattar bland annat skiftarbete, relationer till arbetsgruppen, att bli utsatt för olika typer av hälso- och säkerhetsrisker samt att bemöta död och döende. Det framkom dessutom att besparingar och omstruktureringar var en av de mest frekvent nämnda stressfaktorerna för sjuksköterskor, liksom arbetsbelastning, krav och förväntningar. De konsekvenser som tydligast dök upp genom studiernas resultat handlade om hur sjuksköterskorna kunde drabbas både psykiskt och fysiskt, samt hur det kunde leda till konsekvenser gällande patientsäkerhetsrisker. Slutsats: Litteraturstudiens författare har utifrån resultatet kunnat dra slutsatsen av hur viktig en god arbetsmiljö är för att kunna erbjuda en trygg och säker vård. De faktorer som påverkar sjuksköterskans arbetsrelaterade stress leder inte endast till konsekvenser för hur sjuksköterskan kan drabbas både psykiskt och fysiskt, utan kan även leda till konsekvenser gällande patientsäkerhetsrisker.

Medarbetarundersökningens roll i chefernas arbetsmiljöarbete : En kvalitativ studie hos Trafikverket

En god arbetsmiljö är en lagstadgad rättighet för arbetstagaren enligt Arbetsmiljölagen. Arbetet med att säkerställa en god arbetsmiljö kallas arbetsmiljöarbete och är en nyckelfaktor för att skapa hälsosamma och attraktiva arbetsplatser. Medarbetarundersökningar är en undersökningsmetod i det systematiska arbetsmiljöarbetet men kan också användas i syfte att ta reda på medarbetarnas uppfattningar om sin arbetssituation med målet att skapa verksamhetsförbättringar. I denna studie har syftet varit att undersöka vilken roll som medarbetarundersökningen spelar i chefernas arbetsmiljöarbete. Studien undersökte hur cheferna arbetar med arbetsmiljöarbete och hur arbetet med resultatet från en medarbetarundersökning genomförts. Studien genomfördes på en funktion med tre organisationsnivåer och bygger på 12 kvalitativa intervjuer med funktions-, avdelnings- och enhetschefer. Studien visar att medarbetarundersökningen spelar en viktig roll i chefernas arbetsmiljöarbete på alla organisationsnivåer både som diskussionsunderlag i ledningsgrupper och i dialogen med medarbetarna samt som bevis på att genomförda arbetsmiljöinsatser fungerar. Enhetscheferna använder både medarbetarundersökningen och andra undersökningsmetoder i sitt arbetsmiljöarbete.

Hälsofrämjande friskvårdsarbete : En fallstudie om friskvårdsarbete inom kommunal vård- och omsorgsförvaltning

Under senare år har frågan om ett hållbart arbetsliv blivit allt viktigare. Det innebär att goda arbetsförhållanden kommer att bidra till att människor orkar arbeta fram till pensionen. Friskvård har gått från att enbart vara en förmån till att bli en investering för att främja hälsa. Anställdas hälsa och arbetsmiljö är en del av personalfunktionens övergripande arbete för att behålla personal. Fysiskt krävande yrken som omsorgsarbete är ett stort arbetsmiljöproblem som kräver att organisationer försöker förhindra att arbetsrelaterad sjukdom inträffar. Syftet med detta examensarbete är att undersöka vilka förutsättningarna, ur ett medarbetarperspektiv, organisationer bör ta i beaktande för att skapa ett framgångsrikt arbete med hälsofrämjande friskvårdsaktiviteter inom kommunal vård- och omsorgsförvaltning. Arbetet är begränsat till personal inom Ludvika kommun. Friskvård kan förebygga sjukdom, men det kan också öka individens välbefinnande och kan därmed utgöra en framgångsrik strategi för att skapa hälsosammare arbetsplatser. En kombination av metoder användes för datainsamling, i form av en kvantitativ enkätundersökning och en kvalitativ fokusgruppintervju. Resultaten visade bland annat att majoriteten av de anställda är positiva till friskvård på arbetstid och att det är ett fördelaktigt sätt att öka deltagandet, även för dem som är mindre fysiskt aktiva. Tidsbrist och socialt stöd är skäl till varför fysisk aktivitet är lägre eller uteblir. Förutom att de anställda som har ett individuellt ansvar är det chefer, i samråd med organisationen, som har det övergripande ansvaret för att skapa goda förutsättningar för att främja hälsan.

Factors associated with evidence-based practice among registered nurses in Sweden : a national cross-sectional study

Background: Evidence-based practice (EBP) is emphasized to increase the quality of care and patient safety. EBP is often described as a process consisting of distinct activities including, formulating questions, searching for information, compiling the appraised information, implementing evidence, and evaluating the resulting practice. To increase registered nurses' (RNs') practice of EBP, variables associated with such activities need to be explored. The aim of the study was to examine individual and organizational factors associated with EBP activities among RNs 2 years post graduation. Methods: A cross-sectional design based on a national sample of RNs was used. Data were collected in 2007 from a cohort of RNs, included in the Swedish Longitudinal Analyses of Nursing Education/Employment study. The sample consisted of 1256 RNs (response rate 76%). Of these 987 RNs worked in healthcare at the time of the data collection. Data was self-reported and collected through annual postal surveys. EBP activities were measured using six single items along with instruments measuring individual and work-related variables. Data were analyzed using logistic regression models. Results: Associated factors were identified for all six EBP activities. Capability beliefs regarding EBP was a significant factor for all six activities (OR = 2.6 - 7.3). Working in the care of older people was associated with a high extent of practicing four activities (OR = 1.7 - 2.2). Supportive leadership and high collective efficacy were associated with practicing three activities (OR = 1.4 - 2.0). Conclusions: To be successful in enhancing EBP among newly graduated RNs, strategies need to incorporate both individually and organizationally directed factors.

Kostnader och risker med internationell verksamhet i Mexiko

Detta examensarbetes syfte är att identifiera vilka faktorer som påverkar kostnader och risker som kan finnas vid internationellt företagande i Mexiko utifrån ett perspektiv där företagande samt företagsomgivningar vanligtvis är stöttande och välfungerande. En litteraturstudie utfördes på Internationella verksamheter (International Business), och skapade intervjuunderlaget som användes för att intervjua företagare i Mexiko med erfarenheter av både internationell verksamhet samt USA/EU, och enbart erfarenheter från företagande inom landet. Resultat visar att det finns sex stycken samlingsområden som påverkar kostnader och risker; Politiska faktorer som innebär att drastiska förändringar orsakas i ett lands affärsomgivning, och som negativt påverkar ett företags vinster eller andra mål; Ekonomiska faktorer som innebär att det kostar mer att ha verksamhet på grund av brister inom infrastruktur och stödjande verksamheter; Den ekonomiska utvecklingen; Det finns utbredd korruption hos Mexikanska myndigheter på både lokal- och statsnivå; Ledningsprocesser och metoder kan behöva anpassas enligt kulturellt bestämda arbetsrelaterade värderingar; och Internationella verksamheter bör anställa lokala individer till hjälp att utföra affärer inom en specifik kultur.

Ökat hot och våld i arbetslivet – en följd av förändrade arbetsförhållanden? : En studie utifrån de svenska Arbetsmiljöundersökningarna 1991–2005

Increase in work related violence. A reflection of changes in working conditions? An analysis based on the Swedish Work Environment surveys. Victim surveys from Sweden show that the proportion reporting exposure to work related violence has increased. On the basis of the Swedish Work Environment surveys 1991–2005 this article focuses on the following questions: What kind of situations and working conditions are related to workplace violence? And, has the number of employees exposed to these working conditions increased parallel to the rise of reported workplace violence? Logistic regression analysis shows that some situations and working conditions are indeed related tothe risk of violence. To some extent exposure to these working conditions co-varies with exposure to violence. This result is more prominent for women than for men. Further research is needed to understand how changes in working conditions affect the risk of violence and the development thereof, not least from a gender perspective. Even so, changes in working conditions can not alone explain the increase of reported workplace violence in Sweden during this period. It seems that the influence of changed working conditions offers an interesting complement to criminological theories of broadened definitions and decreasing tolerance against violence in problematizing how an increase in reported workplace violence should and could be understood.