EFFECT OF FOOD SHORTAGE ON GROWTH, ENERGETIC RESERVES MOBILIZATION, AND WATER QUALITY IN JUVENILES OF THE REDCLAW CRAYFISH, CHERAX QUADRICARINATUS, REARED IN GROUPS

The aim of this study is to analyze the impact of food shortage on growth performance, by means of energetic reserves (proteins, glycogen and lipids) mobilization and hepatopancreas cells analysis in C. quadricarinatus juveniles maintained in groups, as well as the effect on culture water quality. Two experiments were performed, each of them with two feeding regimes during 45 days. The Control feeding regime, in which crayfish were fed daily (once a day) throughout the experimental period (DF), and the Cyclic feeding regime, in which juveniles were fed for 2 or 4 days (once a day) followed by 2 or 4 days of food deprivation (2F/2D and 4F/4D, respectively) in repeated cycles. Cyclic feeding influenced growth, biochemical composition from hepatopancreas and muscle, and water quality. Juveniles cyclically fed were unable to maintain a normal growth trajectory during 45 days. Apparent feed conversion ratio, apparent protein efficiency ratio, hepatosomatic index and relative pleon mass were similar in cyclic and daily fed animals and no structural damage was found in the hepatopancreas of juveniles subjected to cyclic feeding. The novelty of this study was the significant accumulation of proteins in pleonal muscle in both cyclic feeding regimes (approx. 18%) suggesting that the storage of this constitutive material during food shortage may be an adaptation for a compensatory growth when food becomes abundant again. The cyclic feeding regimes had a positive effect on water quality decreasing inorganic nitrogen concentration. This was due to the reduction in the amount of animal excretes and feces in the group that received approx. 50% less feed. Additionally, water pH was higher in cyclic feeding tanks, as a result of lower organic matter decomposition and consequent release of CO2. Accordingly, total ammonia in the water was significantly lower for the cyclic feeding regimes compared to their respective controls. This study suggests that the protocol of cyclic feeding could be applied at least 45 days in 1 g juveniles maintained in group conditions, without affecting the energetic reserves and hepatopancreas structure, emphasizing the high tolerance of this species to food restriction.

Adequacy of antenatal care and its relationship with low birth weight in Botucatu, Sao Paulo, Brazil: a case-control study

Background: Birth weight reflects gestational conditions and development during the fetal period. Low birth weight (LBW) may be associated with antenatal care (ANC) adequacy and quality. The purpose of this study was to analyze ANC adequacy and its relationship with LBW in the Unified Health System in Brazil.Methods: A case-control study was conducted in Botucatu, Sao Paulo, Brazil, 2004 to 2008. Data were collected from secondary sources (the Live Birth Certificate), and primary sources (the official medical records of pregnant women). The study population consisted of two groups, each with 860 newborns. The case group comprised newborns weighing less than 2,500 grams, while the control group comprised live newborns weighing greater than or equal to 2,500 grams. Adequacy of ANC was evaluated according to three measurements: 1. Adequacy of the number of ANC visits adjusted to gestational age; 2. Modified Kessner Index; and 3. Adequacy of ANC laboratory studies and exams summary measure according to parameters defined by the Ministry of Health in the Program for Prenatal and Birth Care Humanization.Results: Analyses revealed that LBW was associated with the number of ANC visits adjusted to gestational age (OR = 1.78, 95% CI 1.32-2.34) and the ANC laboratory studies and exams summary measure (OR = 4.13, 95% CI 1.36-12.51). According to the modified Kessner Index, 64.4% of antenatal visits in the LBW group were adequate, with no differences between groups.Conclusions: Our data corroborate the association between inadequate number of ANC visits, laboratory studies and exams, and increased risk of LBW newborns. No association was found between the modified Kessner Index as a measure of adequacy of ANC and LBW. This finding reveals the low indices of coverage for basic actions already well regulated in the Health System in Brazil. Despite the association found in the study, we cannot conclude that LBW would be prevented only by an adequate ANC, as LBW is associated with factors of complex and multifactorial etiology. The results could be used to plan monitoring measures and evaluate programs of health care assistance during pregnancy, at delivery and to newborns, focusing on reduced LBW rates.

Mecanismo de ação do estrógeno no gene Amphiregulin em células MCF7 e MDA-MB-231 via P13K

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Prevalência dos principais vírus respiratórios em bovinos da raça holandesa, no estado do Paraná

Pós-graduação em Medicina Veterinária - FMVZ

Expressão gênica de células do cumulus e oócitos bovinos sbmetidos à maturação un vitro com diferentes macromoléculas, EGF e Butirolactina I

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da prevalência das doenças peri-implantares e do sucesso e sobrevivência de implantes osseointegrados com 8 a 10 anos de função

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Modos de aplicação e reaplicação de calcário nas culturas de milho e soja em plantio direto: Efeito residual

Pós-graduação em Agronomia - FEIS

Sevoflurane induces DNA damage whereas isoflurane leads to higher antioxidative status in anesthetized rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Early loading of 6-mm-short implants with a moderately rough surface supporting single crowns: a prospective 5-year cohort study

AimTo evaluate prospectively the clinical and radiographic outcomes after 5years of early loading of 6-mm implants with a moderately rough (SLActive((R))) surface supporting single crowns in the posterior regions.Material and methodsThirty-five consecutive patients received 40 SLActive((R)) (Straumann) 6-mm implants with a diameter of 4.1mm (n=19) or 4.8mm (n=21). Insertion torque and resonance frequency analysis (RFA) were measured at implant installation. RFA was also measured at abutment connection. SynOcta abutments were tightened with 35Ncm after 6weeks of healing, and single porcelain fuse to metal crowns was cemented within 1week. Implant survival rate and marginal bone loss were evaluated at various time intervals until 5years after loading. The clinical crown/implant ratio was calculated as well.ResultsTwo of 40 implants were lost before loading (incorporation rate 95%), and no further implant loss or technical complications were encountered during the 5-year follow-up period. A mean marginal bone loss of 0.70.6mm was found after 5years of function. The clinical crown/implant ratio increased with time from 1.6 at the delivery of the prosthesis to 2 after 5years of loading.ConclusionSix millimeter implants with a SLActive((R)) moderately rough surface supporting single crowns in the posterior region and loaded after 6-7weeks maintained full function for at least 5year with low marginal bone resorption.

Prevalence of and risk factors for bacterial vaginosis among women of reproductive age attending cervical screening in southeastern Brazil

To determine the prevalence of and risk factors for bacterial vaginosis. A cross-sectional study of women aged 14-54 years attending 18 primary healthcare units in Botucatu, Brazil, for cervical screening was undertaken between September 1, 2012, and January 31, 2013. Data on sociodemographics, sexual behavior, and medical history were obtained by interview. Vaginal swabs were taken to classify the vaginal flora according to the Nugent scoring system. Candida sp. hyphae and infection by Trichomonas vaginalis were also evaluated by microscopy and culture, respectively. Stepwise logistic regression analysis was performed to identify risk factors independently associated with bacterial vaginosis. Among 1519 women included in analyses, 457 (30.1%) had bacterial vaginosis. Variables independently associated with bacterial vaginosis were a single marital status (OR 1.4; 95%CI 1.1-1.8), partner infidelity (OR 1.5; 95%CI 1.2-1.9), abnormal discharge in the previous year (OR 1.5; 95%CI 1.2-2.0), and concurrent trichomoniasis (OR 4.1; 95%CI 1.5-11.5). Current use of hormonal contraception (OR 0.7; 95%CI 0.5-0.9), luteal phase of menstrual cycle (OR 0.8; 95%CI 0.6-0.9), higher income (OR 0.8; 95%CI 0.6-0.9), and vaginal candidiasis (OR 0.5; 95%CI 0.3-0.9) all had protective effects. The prevalence of bacterial vaginosis in the study population is high. The epidemiological data provide evidence of the sexual transmissibility of bacterial vaginosis.

Avaliação comparativa da ancestralidade em mulheres com melasma facial: um estudo transversal

Pós-graduação em Patologia - FMB

Relação lisina digestível e energia metabolizável para leitões em fase pré-inicial de creche

Estudou-se a melhor relação entre lisina digestível (LIS) e energia metabolizável (EM) e efeitos no desempenho e balanço de nitrogênio em leitões na fase de creche. No desempenho foram usados 216 leitões dos 6,90 ± 1,11 kg de peso inicial aos 12 kg, aproximadamente. No metabolismo eram 48 leitões com 9,31 ± 2,09 kg. As concentrações de LIS eram: 1,302; 1,390 e 1,497% e de EM: 3.510, 3.700 e 3.830 kcal/kg, combinadas em um arranjo fatorial 3 × 4 e distribuídas em delineamento de blocos ao acaso. No desempenho dos leitões houve interação LIS em no ganho de peso e conversão alimentar. No ganho de peso a resposta para LIS foi linear crescente nas dietas com 3.510 kcal e 3.830 kcal de EM. Na conversão alimentar observou-se efeito quadrático de LIS nas dietas que continham 3.510 kcal e efeito linear de LIS nas dietas com 3.830 kcal de EM. O efeito quadrático de LIS dentro de 3.510 kcal indicou 1,44% do aminoácido digestível como nível ótimo ou a ingestão de 4,16 g LIS/Mcal de em consumida. A resposta linear positiva na conversão alimentar ao acréscimo de LIS nas dietas com 3.830 kcal em indica maior eficiência dos leitões ao acréscimo do aminoácido quando o nível de energia é alto. No ensaio de metabolismo o aumento de em sugere maior retenção de N, enquanto o acréscimo de LIS sugere menor retenção do N ou na relação Mcal de EM:% LIS. A interação observada nos dois ensaios, todavia, indica que a relação LIS:EM, depende do nível energético e da característica considerada. A variação de LIS e em na dieta indica 4 g LIS/Mcal de em consumida, o que corresponde à ingestão diária aproximada de 7,8 g de LIS.

Efeitos do uso de programas de luz e de triptofano suplementar na dieta sobre o desempenho, comportamento e parâmetros de estresse de leitões recém-desmamados

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Incidência e determinantes de infecção de sítio cirúrgico em hospitais de pequeno porte nas divisões regionais de saúde de Araçatuba, Bauru e Botucatu

Pós-graduação em Doenças Tropicais - FMB

Binômio mãe-filho: estudo epidemiológico e de base populacional desenvolvido em maternidades de baixo e alto risco

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)