Fasciola hepatica surface tegument: glycoproteins at the interface of parasite and host

Fasciola hepatica, commonly known as liver fluke, is a trematode which causes Fasciolosis in ruminants and humans. The outer tegumental coat of F. hepatica (FhTeg) is a complex metabolically active biological matrix that is continually exposed to the host immune system and therefore makes a good vaccine target. F. hepatica tegumental coat is highly glycosylated and helminth-derived immunogenic oligosaccharide motifs and glycoproteins are currently being investigated as novel vaccine candidates. This report presents the first systematic characterisation of FhTeg glycosylation using lectin microarrays to characterise carbohydrates motifs present, and lectin histochemistry to localize these on the F. hepatica tegument. We discovered that FhTeg glycoproteins are predominantly oligomannose oligosaccharides that are expressed on the spines, suckers and tegumental coat of F. hepatica and lectin blot analysis confirmed the abundance of N- glycosylated proteins. While some oligosaccharides are widely distributed on the fluke surface other subsets are restricted to distinct anatomical regions. We selectively enriched for FhTeg mannosylated glycoprotein subsets using lectin affinity chromatography and identified 369 proteins by mass spectrometric analysis. Among these proteins are a number of potential vaccine candidates with known immune modulatory properties including proteases, protease inhibitors, paramyosin, Venom Allergen-like II, Enolase and two proteins, nardilysin and TRIL, that have not been previously associated with F. hepatica Furthermore, we provide a comprehensive insight regarding the putative glycosylation of FhTeg components which could highlight the importance of further studies examining glycoconjugates in host-parasite interactions in the context of F. hepatica infection and the development of an effective vaccine.

Interface-mediated ferroelectric patterning and Mn valency in nano-structured PbTiO3/La0.7Sr0.3MnO3

We employed a multitechnique approach using piezo-force response microscopy and photoemission microscopy to investigate a self-organizing polarization domain pattern in PbTiO3/La0.7Sr0.3MnO3 (PTO/LSMO) nanostructures. The polarization is correlated with the nanostructure morphology as well as with the thickness and Mn valence of the LSMO template layer. On the LSMO dots, the PTO is upwards polarized, whereas outside the nanodots, the polarization appears both strain and interface roughness dependent. The results suggest that the electronic structure and strain of the PTO/LSMO interface contribute to determining the internal bias of the ferroelectric layer.

Surprised by Syntax: Reading the Latinity of Paradise Lost

Stanley Fish in his monumental study argued that the reader of Paradise Lost is “surprised by sin” as he or she in the course of engaging with the text falls, like Adam and Eve, into sin and error and is brought up short. Through a “programme of reader harassment” the experience of the fall is re-enacted in the process of reading, wherein lies the poem’s meaning. And reader response criticism was born. But if for Fish the twentieth-century reader is “surprised by sin,” might not the twenty-first century reader, an all too frequently Latinless reader, be surprised by syntax, a syntax which despite of (or maybe because of) its inherent Latinity and associated linguistic alterity functions as a seductively attractive other? The reader, like Eve, is indeed surprised: enchanted, bemused, seduced by the abundant classicism, by the formal Latinate rhetoric achieved by a Miltonic unison of “Voice and Verse” and also by the language of a Satanic tempter who is—in the pejorative sense of the Latin adjective bilinguis—“double-tongued, deceitful, treacherous.” It is hardly an accident that this adjective (with which Milton qualifies hellish betrayal in his Latin gunpowder epic) was typically applied to the forked tongue of a serpent. This study argues that key to the success of the double-tongued Miltonic serpens bilinguis, is his use and abuse of Latinate language and rhetoric. It posits the possible case that this is mirrored in the linguistic methodology of the poeta bilinguis, the geminus Miltonus? For if, like Eve, the twenty-first century reader of Paradise Lost is surprised by syntax, by the Miltonic use and the Satanic abuse of a Latinate voice, might not he or she also be surprised by the text’s bilingual speaking voice?

A cross-sectional evaluation of community pharmacists’ perceptions of intermediate care and medicines management across the healthcare interface

Background Despite the importance placed on the concept of the multidisciplinary team in relation to intermediate care (IC), little is known about community pharmacists’ (CPs) involvement. 
Objective To determine CPs’ awareness of and involvement with IC services, perceptions of the transfer of patients’ medication information between healthcare settings and views of the development of a CP–IC service. 
Setting Community pharmacies in Northern Ireland. 
Methods A postal questionnaire, informed by previous qualitative work was developed and piloted. 
Main outcome measure CPs’ awareness of and involvement with IC. Results The response rate was 35.3 % (190/539). Under half (47.4 %) of CPs ‘agreed/strongly agreed’ that they understood the term ‘intermediate care’. Three quarters of respondents were either not involved or unsure if they were involved with providing services to IC. A small minority (1.2 %) of CPs reported that they received communication regarding medication changes made in hospital or IC settings ‘all of the time’. Only 9.5 and 0.5 % of respondents ‘strongly agreed’ that communication from hospital and IC, respectively, was sufficiently detailed. In total, 155 (81.6 %) CPs indicated that they would like to have greater involvement with IC services. ‘Current workload’ was ranked as the most important barrier to service development.
Conclusion It was revealed that CPs had little awareness of, or involvement with, IC. Communication of information relating to patients’ medicines between settings was perceived as insufficient, especially between IC and community pharmacy settings. CPs demonstrated willingness to be involved with IC and services aimed at bridging the communication gap between healthcare settings.

The role of Lean at the interface with between operations management and applied services within a large aerospace organisation: a boundary spanning perspective

Increased complexity in large design and manufacturing organisations requires improvements at the operations management (OM)–applied service (AS) interface areas to improve project effectiveness. The aim of this paper is explore the role of Lean in improving the longitudinal efficiency of the OM–AS interface within a large aerospace organisation using Lean principles and boundary spanning theory. The methodology was an exploratory longitudinal case approach including exploratory interviews (n = 21), focus groups (n = 2), facilitated action-research workshops (n = 2) and two trials or experiments using longitudinal data involving both OM and AS personnel working at the interface. The findings draw upon Lean principles and boundary spanning theory to guide and interpret the findings. It was found that misinterpretation, and forced implementation, of OM-based Lean terminology and practice in the OM–AS interface space led to delays and misplaced resources. Rather both OM and AS staff were challenged to develop a cross boundary understanding of Lean-based boundary (knowledge) objects in interpreting OM requests. The longitudinal findings from the experiments showed that the development of Lean Performance measurements and lean Value Stream constructs was more successful when these Lean constructs were treated as boundary (knowledge) objects requiring transformation over time to orchestrate improved effectiveness and in leading to consistent terminology and understanding between the OM–AS boundary spanning team.

Interface de busca do Google e Yahoo: a experiência do usuário sob o olhar do eye tracking

A quantidade de páginas disponibilizadas na Web atingiu um tamanho tão grande que se tornou impossível recuperar informações manualmente, necessitando-se de mecanismos que possam ajudar nesse processo. Nesse contexto, os mecanismos de busca podem ser considerados como uma importante categoria do ciberespaço, especialmente para a área da Ciência da Informação, porque diz respeito à organização do conhecimento nesse ambiente, de tal forma que o Google tem sido considerado a porta de entrada no ciberespaço. Isso evidencia a importância que as interfaces de tais mecanismos podem ter sobre o comportamento informacional das pessoas. Recentes pesquisas mostram que nos últimos anos novos elementos contendo dados estruturados foram inseridos nas páginas de resultados do Google o que pode criar condições para mudanças no comportamento dos usuários. Neste trabalho apresenta-se características da tecnologia de eye tracking e seu uso em User Experience, com a apresentação de alguns resultados obtidos por meio uma investigação experimental, comparando o comportamento de usuários diante das páginas de resultados do Google e Yahoo. Observou-se que nos testes com o Google os participantes precisaram de cerca de 30% a menos de tempo para se decidirem sobre a escolha do link. Acredita-se que os participantes podem ter sofrido influência do elemento conhecido como rich snippet. Os resultados mostram que a interface foi capaz de influenciar o comportamento dos participantes quanto à escolha do melhor link, evidenciando a importância da apresentação dos resultados no processo de tomada de decisão de seus usuários.

Automatic dynamic mask extraction for PIV images containing an unsteady interface, bubbles, and a moving structure

When performing Particle Image Velocimetry (PIV) measurements in complex fluid flows with moving interfaces and a two-phase flow, it is necessary to develop a mask to remove non-physical measurements. This is the case when studying, for example, the complex bubble sweep-down phenomenon observed in oceanographic research vessels. Indeed, in such a configuration, the presence of an unsteady free surface, of a solid–liquid interface and of bubbles in the PIV frame, leads to generate numerous laser reflections and therefore spurious velocity vectors. In this note, an image masking process is developed to successively identify the boundaries of the ship and the free surface interface. As the presence of the solid hull surface induces laser reflections, the hull edge contours are simply detected in the first PIV frame and dynamically estimated for consecutive ones. As for the unsteady surface determination, a specific process is implemented like the following: i) the edge detection of the gradient magnitude in the PIV frame, ii) the extraction of the particles by filtering high-intensity large areas related to the bubbles and/or hull reflections, iii) the extraction of the rough region containing these particles and their reflections, iv) the removal of these reflections. The unsteady surface is finally obtained with a fifth-order polynomial interpolation. The resulted free surface is successfully validated from the Fourier analysis and by visualizing selected PIV images containing numerous spurious high intensity areas. This paper demonstrates how this data analysis process leads to PIV images database without reflections and an automatic detection of both the free surface and the rigid body. An application of this new mask is finally detailed, allowing a preliminary analysis of the hydrodynamic flow.

The symmetric syntax of Japanese complex verbs and Slavic prefixes

We report on a robust symmetry between the Polish and the Japanese sequence of syntactic projections in what superficially looks like different domains, namely verbal prefixes (Polish) and multiple verbs (Japanese). This paralellism strongly supports the thesis about the finegrained sequence of positions in syntax, namely that there exists the functional sequence of syntactic heads (fseq) which is invariantly ordered by UG (see e.g. Cinque (1999)) and the surface differences among particular langauges in the order of elements that instantiate this fseq result solely from movement (not from the variation in fseq itself).

Paths in remnant movement: A single solution to three problems in the Polish OVS syntax

On the one hand it has been advanced that remnant movement (RM) serves as a replacement for head movement and leads to certain permutations in word order while it disallows some others (e.g. Cinque (2005)), on the other hand, little attention has been devoted to the consequences RM has for clausal syntax. In this work, I illustrate one such consequence, namely the rise of crossing and nesting movement dependencies and their reflexes. In particular, I make a case for the existence of massive RM that involves entire clausal subtrees in Polish. The analysis provides a uniform solution to three robust puzzles in the Polish OVS construction in a straightforward way.

User Interface Abstraction for enabling TV set based Inclusive Access to the Information Society

199 p.

Diversity of decapod crustaceans at the interface of unconsolidated seabed areas and rocky shores in tropical/subtropical Brazil

The decapod crustacean assemblages from unconsolidated seabed areas located near rocky shores in the Ubatuba region on the south-east coast of Brazil were analysed by investigating the abundance, richness, diversity and dominance of species. The relationships of the sediment and bottom-water temperature to total species abundance, richness and diversity were also evaluated. Monthly samples were taken from January 1998 through December 1999 at sites in Ubatumirim and Mar Virado. At each locality, three areas were defined as either 'exposed', 'sheltered' or 'island'. The highest species abundance was found in the sheltered area of Ubatumirim and the exposed area of Mar Virado, and during autumn. At Ubatumirim, the highest richness occurred in the exposed area and the highest diversity near the island. At Mar Virado, the highest richness and diversity were found near the island. Autumn and winter collections yielded the highest richness but the lowest diversity. The higher diversities, which were measured near the islands and during spring and summer, were consequences of the greater evenness of the data. The areas with the highest diversity also had the most heterogeneous sediment composition, thus reinforcing the hypothesis that areas with more complex substrata support more diverse faunas.

Spotter: towards a unified search interface in IDEs

Program comprehension requires developers to reason about many kinds of highly interconnected software entities. Dealing with this reality prompts developers to continuously intertwine searching and navigation. Nevertheless, most integrated development environments (IDEs) address searching by means of many disconnected search tools, making it difficult for developers to reuse search results produced by one search tool as input for another search tool. This forces developers to spend considerable time manually linking disconnected search results. To address this issue we propose Spotter, a model for expressing and combining search tools in a unified way. The current implementation shows that Spotter can unify a wide range of search tools. More information about Spotter can be found at scg.unibe.ch/research/moldablespotter

Interface virtual com sensor 3D para interação de pessoas acamadas

A interação homem-máquina tem evoluído significativamente nos últimos anos, a ponto de permitir desenvolver soluções adequadas para apoio a pessoas que possuem um certo tipo de limitação física ou cognitiva. O desenvolvimento de técnicas naturais e intuitivas de interação, as chamadas Natural User Interface (NUI), permitem, hoje, que pessoas que estejam acamadas e/ou com incapacidade motora possam executar um conjunto de ações por intermédio de gestos, aumentando assim a sua qualidade de vida. A solução implementada neste projecto é baseada em processamento de imagem e visão por computador através do sensor 3D Kinect e consiste numa interface natural para o desenvolvimento de uma aplicação que reconheça gestos efetuados por uma mão humana. Os gestos identificados pela aplicação acionam um conjunto de ações adequados a uma pessoa acamada, como, por exemplo, acionar a emergência, ligar ou desligar a TV ou controlar a inclinação da cama. O processo de desenvolvimento deste projeto implicou várias etapas. Inicialmente houve um trabalho intenso de investigação sobre as técnicas e tecnologias consideradas importantes para a realização do trabalho - a etapa de investigação, a qual acompanhou praticamente todo o processo. A segunda etapa consistiu na configuração do sistema ao nível do hardware e do software. Após a configuração do sistema, obtiveram-se os primeiros dados do sensor 3D Kinect, os quais foram convertidos num formato mais apropriado ao seu posterior tratamento. A segmentação da mão permitiu posteriormente o reconhecimento de gestos através da técnica de matching para os seis gestos implementados. Os resultados obtidos são satisfatórios, tendo-se contabilizado cerca de 96% de resultados válidos. A área da saúde e bem-estar tem necessidade de aplicações que melhorem a qualidade de vida de pessoas acamadas, nesse sentido, o protótipo desenvolvido faz todo o sentido na sociedade actual, onde se verifica o envelhecimento da população.

Electrospun scaffolds for regeneration of musculoskeletal interface tissues

L’apparato muscolo scheletrico è composto da strutture muscolari, articolari e ossee. Tali tessuti sono molto diversi tra loro e hanno proprietà meccaniche estremamente variabili, pertanto presentano una transizione graduale in corrispondenza della loro giunzione, onde evitare l’insorgere di concentrazioni di tensione. L’evoluzione ha portato alla formazione di particolari interfacce che permettono la corretta trasmissione dei carichi distribuendo le tensioni su una superficie più ampia in corrispondenza della giunzione. Le interfacce che vanno a inserirsi nell’osso vengono definite entesi e in particolare, in questa review, analizzeremo il caso di quelle tra tendini/legamenti e osso. In questo lavoro ci siamo anche concentrati sulla giunzione miotendinea, ovvero tra muscolo e tendine. Sono numerose le lesioni che riguardano muscoli, ossa, tendini o legamenti e molto spesso l’infortunio avviene a livello della giunzione. Quando ciò accade vi sono diverse strade, ciascuna con i suoi vantaggi e svantaggi: sutura, autograft, allograft o xenograft. Oltre a queste soluzioni si è fatta gradualmente più spazio la possibilità di realizzare degli scaffold che vadano temporaneamente a sostituire la parte danneggiata e a promuovere la sua rigenerazione, degradandosi man mano. L’elettrofilatura (Elettrospinning) è un processo produttivo che negli ultimi decenni si è affermato come tecnica per la fabbricazione di questi scaffold, fino a diventare uno tra i principali processi utilizzati dai ricercatori in questo campo. Questa tecnica infatti permette di realizzare scaffold di nanofibre porose utilizzando polimeri biodegradabili e soprattutto biocompatibili. Lo scopo della review è proprio quello di scoprire tutti i lavori e gli studi che utilizzano l’elettrofilatura per realizzare degli scaffold per interfacce, delineando così lo stato dell’arte sui progressi fatti e sulle varie tecniche utilizzate.

Analysis and development of interface components and procedures for the design and commissioning toolchains in automation systems

The objective of the thesis project, developed within the Line Control & Software Engineering team of G.D company, is to analyze and identify the appropriate tool to automate the HW configuration process using Beckhoff technologies by importing data from an ECAD tool. This would save a great deal of time, since the I/O topology created as part of the electrical planning is presently imported manually in the related SW project of the machine. Moreover, a manual import is more error-prone because of human mistake than an automatic configuration tool. First, an introduction about TwinCAT 3, EtherCAT and Automation Interface is provided; then, it is analyzed the official Beckhoff tool, XCAD Interface, and the requirements on the electrical planning to use it: the interface is realized by means of the AutomationML format. Finally, due to some limitations observed, the design and implementation of a company internal tool is performed. Tests and validation of the tool are performed on a sample production line of the company.