Heat flow determinations of ODP Hole 111-504B

Hole 504B in the eastern equatorial Pacific has been the focus of five scientific drilling expeditions since it was first drilled in 1979. During these five legs, a series of temperature logs has been obtained over a time span of almost 8 yr, documenting the geothermal and hydrologic state of the oceanic crust in this region. Immediately following reentry at the onset of ODP Leg 111 operations, a high-resolution temperature probe was lowered into the borehole and a precise record of temperature vs. depth in Hole 504B was recorded down to 1300 mbsf. As was observed during previous legs, the temperature gradient in the upper 400 m was reduced, indicating that downhole flow of cool ocean waters through the casing continued, though at a diminished rate. As subhydrostatic pressures in the upper basement have gradually diminished, the volume of flow has decayed from an estimated 6000-7000 L/hr in late 1979 to about 80 L/hr during Leg 111. At depths below 480 mbsf, a predominantly conductive heat transfer environment enabled the temperature gradient log to be analyzed with respect to lithology on both fine and broad scales. Anomalies in the gradient log in the cased section through the sedimentary column were found to correspond to biostratigraphic age markers and/or sharp changes in sediment composition and texture. Broad variations in temperature gradient within the basement correlated with large-scale porosity trends. Conductive heatflow estimates depict a systematic reduction with depth, ranging from approximately 196 mW/m**2 in the sediments to 120 ± 17 mW/m**2 at 1300 mbsf. Possible causes for this observation were examined from several perspectives, but none was suitably convincing. A fluid instability analysis indicated the likely existence of convection cells within the borehole and substantiated the hypothesis of mixing within the borehole postulated from isotopic and chemical studies of borehole waters. However, such mixing of borehole fluids does not provide an adequate explanation for the heatflow variations, and the disparity between surficial and deep values of heat flow remains unresolved.

University of Michigan 150-pound football team, 1948

Top Row: Dick Bodycomb, Gus Eaton, Bud Marshall, Tony Cybulski, Herb Taggart, Bob Kash, Burl Selden (ass't coach), Cliff Keen (coach).

Third Row: Gene Kiddon, Gordon Smith, Vaughn Lowther, Jim Coleman, Larry Shaw, James Johnson, Frank Whitehouse, Gene Freed, Everet Knapp, George Sipp, Stan Emerling.

Second Row: Bill Clark, James Richter, Jack Keller, Dave Bradbury Fred Koehn, Hyman Berman, Bill Parshall, Bill Connoly, M. Richard Fleishman, Frank Murphy, Jim Briskin, Leon Hinz.

Bottom Row: John Wilcox, Prentice Ryan, Ed Morey, Jim Costa, Barry Breakey, Jere Ogle, Jim Armelagos, Don O'Connell, Jerry Burns, John Picard, James Sakai, Ted Karmazin.

Yudaya minzoku kenkyū shiryō /

Confidential document stamped "gokuhi".

Taiwan shihō

Mode of access: Internet.

1947 150 Pound Football team, UM Football

Top Row (left to right): ? Nelson*, Aram Nahabedian*, Robert Hicks, George Strong, Bradshaw McKee, James Costa, John Olson, George Sipp, Gene Kiddon, Don O'Connell, Doug Wicks.

Third Row: Barry Breakey, Ross Marshall, Byron Parshall, George Bradley, Herbert Hurell, Leon Hinz, Eugene Freed, James Smith, William Clark, Meryl Englander, Edwin Morey, Edward Rosatti.

Second: Dave Bradbury, James Sakai, Russell Buster, Robert Rodgers, assistant coach George Allen, captain, Charlie Ketterer, head coach Cliff Keen, Dick Mandeville*,? Singer*, Frank Whitehouse,

Bottom Row: John Wilcox*, Larry Shaw, ? Schnider*,? Budick*, Stanley Emerling*

*Did not earn 150 pound letter

Letterwinners not pictured: John Allred

Systematic expression profiling of the mouse transcriptome using RIKEN cDNA mircoarrays

The number of known mRNA transcripts in the mouse has been greatly expanded by the RIKEN Mouse Gene Encyclopedia project. Validation of their reproducible expression in a tissue is an important contribution to the study of functional genomics. In this report, we determine the expression profile of 57,931 clones on 20 mouse tissues using cDNA microarrays. Of these 57,931 clones, 22,928 clones correspond to the FANTOM2 clone set. The set represents 20,234 transcriptional units (TUs) out of 33,409 TUs in the FANTOM2 set. We identified 7206 separate clones that satisfied stringent criteria for tissue-specific expression. Gene Ontology terms were assigned for these 7206 clones, and the proportion of 'molecular function' ontology for each tissue-specific clone was examined. These data will provide insights into the function of each tissue. Tissue-specific gene expression profiles obtained using our cDNA microarrays were also compared with the data extracted from the GNF Expression Atlas based on Affymetrix microarrays. One major outcome of the RIKEN transcriptome analysis is the identification of numerous nonprotein-coding mRNAs. The expression profile was also used to obtain evidence of expression for putative noncoding RNAs. In addition, 1926 clones (70%) of 2768 clones that were categorized as unknown EST, and 1969 (58%) clones of 3388 clones that were categorized as unclassifiable were also shown to be reproducibly expressed.

An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination

Several pathogenic strains of Escherichia coli exploit type III secretion to inject effector proteins into human cells, which then subvert eukaryotic cell biology to the bacterium's advantage. We have exploited bioinformatics and experimental approaches to establish that the effector repertoire in the Sakai strain of enterohemorrhagic E. coli (EHEC) O157:H7 is much larger than previously thought. Homology searches led to the identification of > 60 putative effector genes. Thirteen of these were judged to be likely pseudogenes, whereas 49 were judged to be potentially functional. In total, 39 proteins were confirmed experimentally as effectors: 31 through proteomics and 28 through translocation assays. At the protein level, the EHEC effector sequences fall into > 20 families. The largest family, the NleG family, contains 14 members in the Sakai strain alone. EHEC also harbors functional homologs of effectors from plant pathogens (HopPtoH, HopW, AvrA) and from Shigella (OspD, OspE, OspG), and two additional members of the Map/IpgB family. Genes encoding proven or predicted effectors occur in > 20 exchangeable effector loci scattered throughout the chromosome. Crucially, the majority of functional effector genes are encoded by nine exchangeable effector loci that lie within lambdoid prophages. Thus, type III secretion in E. coli is linked to a vast phage metagenome, acting as a crucible for the evolution of pathogenicity.

Engaging tutors in using e-repositories for learning and teaching

Universities which set up online repositories for the management of learning and teaching resources commonly find that uptake is poor. Tutors are often reluctant to upload their materials to e-repositories, even though the same tutors are happy to upload resources to the virtual learning environment (e.g. Blackboard, Moodle, Sakai) and happy to upload their research papers to the university’s research publications repository. The paper reviews this phenomenon and suggests constructive ways in which tutors can be encouraged to engage with an e-repository. The authors have recently completed a major project “Developing Repositories at Worcester” which is part of a group of similar projects in the UK. The paper includes the feedback and the lessons learned from these projects, based on the publications and reports they have produced. They cover ways of embedding repository use into institutional working practice, and give examples of different types of repository designed to meet the needs of those using different kinds of learning and teaching resources. As well as this specific experience, the authors summarise some of the main findings from UK publications, in particular the December 2008 report of Joint Information Systems Committee: Good intentions: improving the evidence base in support of sharing learning materials and Online Innovation in Higher Education, Ron Cooke’s report to a UK government initiative on the future of Higher Education. The issues covered include the development of Web 2.0 style repositories rather than conventionally structured ones, the use of tags rather than metadata, the open resources initiative, the best use for conventional repositories, links to virtual learning environments, and the processes for the management and support of repositories within universities. In summary the paper presents an optimistic, constructive view of how to embed the use of e-repositories into the working practices of university tutors. Equally, the authors are aware of the considerable difficulties in making progress and are realistic about what can be achieved. The paper uses evidence and experience drawn from those working in this field to suggest a strategic vision in which the management of e-learning resources is productive, efficient and meets the needs of both tutors and their students.