Neural representations of courtship song in the Drosophila brain.

Acoustic communication in drosophilid flies is based on the production and perception of courtship songs, which facilitate mating. Despite decades of research on courtship songs and behavior in Drosophila, central auditory responses have remained uncharacterized. In this study, we report on intracellular recordings from central neurons that innervate the Drosophila antennal mechanosensory and motor center (AMMC), the first relay for auditory information in the fly brain. These neurons produce graded-potential (nonspiking) responses to sound; we compare recordings from AMMC neurons to extracellular recordings of the receptor neuron population [Johnston's organ neurons (JONs)]. We discover that, while steady-state response profiles for tonal and broadband stimuli are significantly transformed between the JON population in the antenna and AMMC neurons in the brain, transient responses to pulses present in natural stimuli (courtship song) are not. For pulse stimuli in particular, AMMC neurons simply low-pass filter the receptor population response, thus preserving low-frequency temporal features (such as the spacing of song pulses) for analysis by postsynaptic neurons. We also compare responses in two closely related Drosophila species, Drosophila melanogaster and Drosophila simulans, and find that pulse song responses are largely similar, despite differences in the spectral content of their songs. Our recordings inform how downstream circuits may read out behaviorally relevant information from central neurons in the AMMC.

Gamma-ray spectroscopy at TRIUMF-ISAC

The 8π spectrometer at TRIUMF-ISAC consists of 20 Compton-suppressed germanium detectors and various auxiliary devices. The Ge array, once used for studies of nuclei at high angular momentum, has been transformed into the world's most powerful device dedicated to radioactive-decay studies. Many improvements in the spectrometer have been made, including a high-throughput data acquisition system, installation of a moving tape collector, incorporation of an array of 20 plastic scintillators for β-particle tagging, 5 Si(Li) detectors for conversion electrons, and 10 BaF2 detectors for fast-lifetime measurements. Experiments can be performed where data from all detectors are collected simultaneously, resulting in a very detailed view of the nucleus through radioactive decay. A number of experimental programmes have been launched that take advantage of the versatility of the spectrometer, and the intense beams available at TRIUMF-ISAC. © 2006 American Institute of Physics.

MgB2 sample tests for possible applications of superconducting fault current limiters

Cheap to make and easy to shape, Magnesium Diboride (MgB2) throws the field of applied superconductivity wide open. Great efforts have been made to develop a super-conducting fault current limiter (SFCL) using MgB 2. With a superconducting transition temperature of 39 K, MgB 2 can be conveniently cooled with commercial cryocoolers. A cryogenic desktop test system, an ac pulse generation system and a real time data acquisition program in LabView/DAQmx were developed to investigate the quench behavior of MgB2 wires under pulse overcurrents at 25 K in self-field conditions. The experimental results on the current limitation behavior show the possibilities for using MgB2 for future SFCL applications. © 2007 IEEE.

Investigations of current limiting properties of the MgB2 wires subjected to pulse overcurrents in the benchtop tester

A laboratory scale desktop test system including a cryogenic system, an AC pulse generation system and a real time data acquisition program in LabView/DAQmx, has been developed to evaluate the quench properties of MgB 2 wires as an element in a superconducting fault current limiter under pulse overcurrents at 25K in self-field conditions. The MgB2 samples started from a superconducting state and demonstrated good current limiting properties characterized by a fast transition to the normal state during the first half of the cycle and a continuously limiting effect in the subsequent cycles without burnouts. The experimental and numerical simulation results on the quench behaviour indicate the feasibility of using MgB 2 for future superconducting fault current limiter (SFCL) applications. © IOP Publishing Ltd.

A critical analysis of loudness calculation methods

The physical meaning and calculation procedures for determining loudness was critically analyzed. Four noise sources were used in comparing the software packages dBFA dBSonic, which were used in the investigation to a public domain code. The purpose of the comparison was to evaluate the validity of the results obtained and to gain an idea of the shortcomings of the relevant standards. A comparison of the results for loudness was computed from various methods, used in the study. Two basic sources of input data such as a sound level meter (SLM) and a 01 dB data acquisition system (DAQ), were available for the comparison. The SLM directly gave 1/3 octave band levels, while the data from the DAQ was filtered to give the results. Five processing methods, including a Visual Basic (VB) program and a VB program adapted from dBFA, were used for the study. It was found that the calculation of loudness from 1/3 octave cannot be separated from the filtering process.

Encoding of mixtures in a simple olfactory system.

Natural odors are usually mixtures; yet, humans and animals can experience them as unitary percepts. Olfaction also enables stimulus categorization and generalization. We studied how these computations are performed with the responses of 168 locust antennal lobe projection neurons (PNs) to varying mixtures of two monomolecular odors, and of 174 PNs and 209 mushroom body Kenyon cells (KCs) to mixtures of up to eight monomolecular odors. Single-PN responses showed strong hypoadditivity and population trajectories clustered by odor concentration and mixture similarity. KC responses were much sparser on average than those of PNs and often signaled the presence of single components in mixtures. Linear classifiers could read out the responses of both populations in single time bins to perform odor identification, categorization, and generalization. Our results suggest that odor representations in the mushroom body may result from competing optimization constraints to facilitate memorization (sparseness) while enabling identification, classification, and generalization.

Encoding of mixtures in a simple olfactory system

Natural odors are usually mixtures; yet, humans and animals can experience them as unitary percepts. Olfaction also enables stimulus categorization and generalization. We studied how these computations are performed with the responses of 168 locust antennal lobe projection neurons (PNs) to varying mixtures of two monomolecular odors, and of 174 PNs and 209 mushroom body Kenyon cells (KCs) to mixtures of up to eight monomolecular odors. Single-PN responses showed strong hypoadditivity and population trajectories clustered by odor concentration and mixture similarity. KC responses were much sparser on average than those of PNs and often signaled the presence of single components in mixtures. Linear classifiers could read out the responses of both populations in single time bins to perform odor identification, categorization, and generalization. Our results suggest that odor representations in the mushroom body may result from competing optimization constraints to facilitate memorization (sparseness) while enabling identification, classification, and generalization

Rapid patterning of 1-D collagenous topography as an ECM protein fibril platform for image cytometry.

Cellular behavior is strongly influenced by the architecture and pattern of its interfacing extracellular matrix (ECM). For an artificial culture system which could eventually benefit the translation of scientific findings into therapeutic development, the system should capture the key characteristics of a physiological microenvironment. At the same time, it should also enable standardized, high throughput data acquisition. Since an ECM is composed of different fibrous proteins, studying cellular interaction with individual fibrils will be of physiological relevance. In this study, we employ near-field electrospinning to create ordered patterns of collagenous fibrils of gelatin, based on an acetic acid and ethyl acetate aqueous co-solvent system. Tunable conformations of micro-fibrils were directly deposited onto soft polymeric substrates in a single step. We observe that global topographical features of straight lines, beads-on-strings, and curls are dictated by solution conductivity; whereas the finer details such as the fiber cross-sectional profile are tuned by solution viscosity. Using these fibril constructs as cellular assays, we study EA.hy926 endothelial cells' response to ROCK inhibition, because of ROCK's key role in the regulation of cell shape. The fibril array was shown to modulate the cellular morphology towards a pre-capillary cord-like phenotype, which was otherwise not observed on a flat 2-D substrate. Further facilitated by quantitative analysis of morphological parameters, the fibril platform also provides better dissection in the cells' response to a H1152 ROCK inhibitor. In conclusion, the near-field electrospun fibril constructs provide a more physiologically-relevant platform compared to a featureless 2-D surface, and simultaneously permit statistical single-cell image cytometry using conventional microscopy systems. The patterning approach described here is also expected to form the basics for depositing other protein fibrils, seen among potential applications as culture platforms for drug screening.

Dual-wavelength photochromic fulgides for parallel recording memory

Two photochromic fulgides, 2-{2-[4-(N,N-dimethylnilino)-5-methyl-4-oxazoly]}ethylidene-4-(1-methylethylidene) tetrahydrofuran-2,5-dione (A) and 3-(1,2-dimethyl-5-phenyl-3-pyrolloethylidene)-4-(1-methylethylidene)tetrahydrofuran-2,5-dione (B), doped in PMMA as candidates of dual-wavelength optical memory for parallel recording has been investigated. With 488 nm-laser and 650 nm-laser, both "cross" and "star" images are recorded on the fulgides-PMMA film and read out clearly, respectively. Crosstalk between two fulgides in PMMA matrix and nondestructive readout has also been explored. The results show that no significant cross-talk is detected between them, and nondestructive readout is up to 201 times. (C) 2005 Elsevier B.V. All rights reserved.

基于ACEX1K30的HIRFL-CSRe高精度线性插值异步FIFO

本设计实现了HIRFL-CSRe同步系统控制器DSP插件内的FPGA中的FIFO(First in first out)功能,数据入口是16位DSP总线,数据出口是16位DAC总线。其核心机制采用双缓冲"乒乓操作",并在FPGA内完成一次线性插值。程序采用VHDL硬件描述语言在Altera公司的现场可编程逻辑器件ACEX1K30上实现。FIFO实现机制完全自行设计,解决了传统异步FIFO由于读写时钟异步造成的空/满标志难以准确给出及数据输出时间不能精确保证的难题,满足了HIRFL-CSRe对于输出数据不间断(每微秒一个)的要求,并由于在FPGA内实现了一次线性插值,从而把从DSP中接收到的已插值数据量增加了一倍,在宏观上降低了DSP的数据运算量。模块经现场工作证实FIFO数据输出时间误差控制在40ns内,达到设计要求。

基于FPGA的VME总线控制器及其驱动程序

本文论述了用于兰州重离子加速器冷却储存环(HIRFL-CSR)前端控制系统的总线控制器的FPGA设计及其基于ARM-Linux下的驱动程序设计。该总线控制器采用Altera公司的ACEX系列中的EP1K30实现,通过VME总线背板同其它VME设备(CPLD)通信,可读VME设备数据,监视电源运行状况,也可向VME设备发送命令和写数据,并且能够响应VME设备中断,读中断数据。为了能够通过AT91RM9200控制器访问VME总线控制器,必须为其编写相应的驱动程序。驱动程序定义了应用程序调用接口和数据格式,并实现了中断机制、多进程访问和数据的突发(burst)读写。

An online monitor ionization chamber used in particle therapy

The clinical trials of tumor therapy using heavy ions beam C-12 are now in progress at Institute of Modem Physics in Lanzhou. In order to achieve the precise radiotherapy with the high energy C-12 beam in active pencil beam scanning mode, we have developed an ionization chamber(IC) as an online monitor for beam intensity and also a dosimeter after calibration. Through the choosing of working gas and voltage, optimizing of the electrics and the read-out system, calibrating the linearity, the detector system provide us one of the simple and highly reliable way to monitoring the beam during the active pencil beam scanning treatments. The measurement results of this detector system show that it could work well under the condition of high energy C-12 beam in active pencil beam scanning mode.

Development of multi-channel gated integrator and PXI-DAQ system for nuclear detector arrays

A multi-channel gated integrator and PXI based data acquisition system have been developed for nuclear detector arrays with hundreds of detector units. The multi-channel gated integrator can be controlled by a programmable Cl controller. The PXI-DAQ system consists of NI PXI-1033 chassis with several PXI-DAQ cards. The system software has a user-friendly GUI which is written in C language using LabWindows/CVI under Windows XP operating system. The performance of the PXI-DAQ system is very reliable and capable of handling event rate up to 40 kHz. (C) 2010 Elsevier B.V. All rights reserved.

Dynamic Measurement System for Dipoles of RCS for CS

A dynamic measurement system was developed by the Institute of Modern Physics (IMP) for the dipole prototype of Rapid Cycle Synchrotron (RCS) of China Spallation Neutron Source (CSNS). The repetition frequency of RCS is 25 Hz. The probe is a moving arc searching-coil, and the data acquisition system is based on the dynamic analysis modular of National Instrument. To get the error of high order harmonics of the field at basic frequency, the hardware integrator is replaced by a high speed ADC with software filter and integrator. A series of harmonic coefficients of field are used to express the varieties of dynamic fields in space and time simultaneously. The measurement system has been tested in Institute of High Energy Physics (IHEP), and the property of the dipole prototype of RCS has been measured. Some measurement results and the repeatability of system are illustrated in this paper.

光谱测量与能级寿命测量的数据获取电子学系统

随着物理实验要求的提高，利用先进的计算机技术来改造现有的物理实验设备具有重要意义。因此，我们设计研制了光谱测量与能级寿命测量数据获取电子学系统。本文全面论述了这一系统的组成结构。本系统由微机进行控制，通过基于Pentium机上工SA总线的接口电路来控制数据采集过程，实现了测量设备的智能化，并具有高速度、高可靠性的特点。配合软件工作可完全实现实验的无人监控。论文第一部分介绍了这个系统开发的背景及意义。第二部分是这个系统的组成和硬件结构、性能。这个系统完成的主要功能是：（1）采集数据并处理；（2）控制外设马达的运动；（3）显示系统的状态。第三部分是调试过程以及针对在调试中出现的各种实际问题提出的解决办法和预防措施。在设计工作完成之后，本系统在兰州近代物理研究所的加速器实验大厅中进行了模拟实验，并采集了部分数据。通过对获取数据的处理，获得了比较满意的结果。在论文的最后一部分中给出了本系统在实际运行后得出的实验结果以及此系统中可待完善之处。