Safety and effectiveness of nurse telephone consultation in out of hours primary care: randomised controlled trial

Objective To determine the safety and effectiveness of nurse telephone consultation in out of hours primary care by investigating adverse events and the management of calls.

Identification of sample-specific sequences in mammalian cDNA and genomic DNA by the novel ligation-mediated subtraction (Limes)

The representational difference analysis (RDA) and other subtraction techniques are used to enrich sample-specific sequences by elimination of ubiquitous sequences existing in both the sample of interest (tester) and the subtraction partner (driver). While applying the RDA to genomic DNA of cutaneous lymphoma cells in order to identify tumor relevant alterations, we predominantly isolated repetitive sequences and artificial repeat-mediated fusion products of otherwise independent PCR fragments (PCR hybrids). Since these products severely interfered with the isolation of tester-specific fragments, we developed a considerably more robust and efficient approach, termed ligation-mediated subtraction (Limes). In first applications of Limes, genomic sequences and/or transcripts of genes involved in the regulation of transcription, such as transforming growth factor β stimulated clone 22 related gene (TSC-22R), cell death and cytokine production (caspase-1) or antigen presentation (HLA class II sequences), were found to be completely absent in a cutaneous lymphoma line. On the assumption that mutations in tumor-relevant genes can affect their transcription pattern, a protocol was developed and successfully applied that allows the identification of such sequences. Due to these results, Limes may substitute/supplement other subtraction/comparison techniques such as RDA or DNA microarray techniques in a variety of different research fields.

Antigenic peptides containing large PEG loops designed to extend out of the HLA-A2 binding site form stable complexes with class I major histocompatibility complex molecules.

Recognition of peptides bound to class I major histocompatibility complex (MHC) molecules by specific receptors on T cells regulates the development and activity of the cellular immune system. We have designed and synthesized de novo cyclic peptides that incorporate PEG in the ring structure for binding to class I MHC molecules. The large PEG loops are positioned to extend out of the peptide binding site, thus creating steric effects aimed at preventing the recognition of class I MHC complexes by T-cell receptors. Peptides were synthesized and cyclized on polymer support using high molecular weight symmetrical PEG dicarboxylic acids to link the side chains of lysine residues substituted at positions 4 and 8 in the sequence of the HLA-A2-restricted human T-lymphotrophic virus type I Tax peptide. Cyclic peptides promoted the in vitro folding and assembly of HLA-A2 complexes. Thermal denaturation studies using circular dichroism spectroscopy showed that these complexes are as stable as complexes formed with antigenic peptides.

Taking The 'Sport' Out of Sports Parenting: Toward A Theory of Sport Related Parent-Child Communication Competency

Research has shown that over-emphasis on winning is the number one reason why approximately seventy percent of the forty million children who participate in youth sports will quit by age 13. This study utilized a constructivist grounded theory approach to investigate the role of parent-child communication within the context of youth sports. A total of 22 athletes and 20 parents were recruited through a Western university to discuss messages exchanged during youth sport participation. The results suggest that the delineation between messages of support and pressure is largely dependent on discursive work done by both parent and child. Parents who employed competent communicative strategies to avoid miscommunications regarding participation and sports goals were able to provide support and strengthen the relationship despite pressurized situations. The present study frames the youth sport dilemma within a developing conceptualization of communicative (in)competence and offers theoretical implications for sport related parent-child communication competency (SRPCCC).

The Psychology of Adolescent Out-of Wedlock Pregnancy and Childbearing

Illegitimate adolescent pregnancy creates a variety of problems, beginning with the difficult decision about whether or not to terminate the pregnancy. If the pregnancy is carried to term, choices follow regarding marriage or single parenthood and keeping or relinquishing the child. All of these choices involve consequences for the adolescent, many of them negative ones. This paper examines the problem of out-of-wedlock teen pregnancy and its possible psychological sources. It also introduces a method for analyzing the psychology of unwed teen pregnancy and childbearing and reviews the literature on the subject by this method. NOTE: Approvals page submitted to digital archive lacks signatures

Out of the ordinary : Anne Ryan and collage

Anne Ryan (1889-1954) was an active member of New York’s vibrant avant-garde art community during the tumultuous period marked by World War II. Ryan participated in the famed 1951 “Ninth Street Show,” and was an early member of Betty Parsons’s legendary stable of artists. She is not widely known today, however, and her influence is rarely acknowledged. Ryan is primarily known for her abstract collages—works that are frequently linked to Abstract Expressionism.

Confession of students for walking out of commons, April 1807

This document, signed by 108 students, contains a confession for their part in the 1807 student rebellion. Students were required to sign this confession in order to avoid expulsion.