Salmonid behaviour under winter conditions

Winter conditions are believed to play an important role in the population dynamics of northern temperate stream fish, challenging the ability of fish to physiologically and behaviourally adapt. Climate change is predicted to increase both mean temperature and temperature fluctuations, especially during winter, leading to dynamic environmental conditions in terms of river ice production and flow. Therefore, knowledge about the winter ecology of stream fish is important for predicting and mitigating anthropogenic impacts on fish production in boreal streams. Stream salmonids are relatively active throughout winter, and behavioural responses to different winter conditions may be critical for survival. Yet, relatively little is known about overwintering behaviour of salmonids, particularly in streams with ice. In this doctoral thesis, I report the results from experimental field and laboratory studies on the behavioural ecology of juvenile salmonids under winter conditions. My results from the field show that salmonids grow more and use a broader range of habitats in the presence of surface ice than in its absence. Results from the laboratory experiments show that the presence of surface ice increases food intake rates, reduces stress and affects social interactions. These laboratory results may explain the positive effects of ice cover on growth that was found in the field experiment. Moreover, I show that drift-feeding ability is reduced at low temperatures, and that nocturnal drift foraging under winter conditions has a low efficiency.

Diversity and Stability in Food Webs : Impacts of Non-Equilibrium Dynamics, Topology and Variation

With progressive climate change, the preservation of biodiversity is becoming increasingly important. Only if the gene pool is large enough and requirements of species are diverse, there will be species that can adapt to the changing circumstances. To maintain biodiversity, we must understand the consequences of the various strategies. Mathematical models of population dynamics could provide prognoses. However, a model that would reproduce and explain the mechanisms behind the diversity of species that we observe experimentally and in nature is still needed. A combination of theoretical models with detailed experiments is needed to test biological processes in models and compare predictions with outcomes in reality. In this thesis, several food webs are modeled and analyzed. Among others, models are formulated of laboratory experiments performed in the Zoological Institute of the University of Cologne. Numerical data of the simulations is in good agreement with the real experimental results. Via numerical simulations it can be demonstrated that few assumptions are necessary to reproduce in a model the sustained oscillations of the population size that experiments show. However, analysis indicates that species "thrown together by chance" are not very likely to survive together over long periods. Even larger food nets do not show significantly different outcomes and prove how extraordinary and complicated natural diversity is. In order to produce such a coexistence of randomly selected species—as the experiment does—models require additional information about biological processes or restrictions on the assumptions. Another explanation for the observed coexistence is a slow extinction that takes longer than the observation time. Simulated species survive a comparable period of time before they die out eventually. Interestingly, it can be stated that the same models allow the survival of several species in equilibrium and thus do not follow the so-called competitive exclusion principle. This state of equilibrium is more fragile, however, to changes in nutrient supply than the oscillating coexistence. Overall, the studies show, that having a diverse system means that population numbers are probably oscillating, and on the other hand oscillating population numbers stabilize a food web both against demographic noise as well as against changes of the habitat. Model predictions can certainly not be converted at their face value into policies for real ecosystems. But the stabilizing character of fluctuations should be considered in the regulations of animal populations.

Materiais electrocrómicos e mecaelectroquímicos: contextualização e experimentação na disciplina de química do 12º ano

Durante os últimos 1O anos, em particular, tem-se assistido à descoberta e desenvolvimento de materiais com propriedades únicas e potencialmente úteis em vários domínios da Ciência e Tecnologia. Quer seja na área das Ciências Biomédicas e do Ambiente, na área das Novas Tecnologias de Informação e Comunicação ou nas Novas Tecnologias relacionadas com sistemas de Conversão e Armazenamento de Energia Eléctrica, encontram-se inúmeros exemplos de Novos Materiais com propriedades muito interessantes, e cujas aplicações inovadoras podem revolucionar o nosso Mundo. O presente trabalho é uma proposta de contextualização e experimentação do tema "Materiais Electrocrómicos e Mecaelectroquímicos" no Programa de Química de 12° Ano, nomeadamente na terceira Unidade - Plásticos, Vidros e Novos Materiais. Neste, é apresentada uma sub-unidade que possa integrar a unidade referida. Faz-se especial referência às aplicações dos polímeros condutores, que pela sua inovação e actualidade tomam a leccionação do tema mais apelativa, interessante e facilitadora de uma literacia científica. São apresentadas experiências laboratoriais demonstrativas e elucidativas do tema em questão. Como forma de apoiar a leccionação do referido tema, é apresentada a proposta de uma animação gráfica animada em Power Point. ABSTRACT: During the past 1O years, in particular, it has witnessed to the discovery and development of materials with unique properties and potentially useful in various fields of Science and Technology. There are numerous examples of new materials in different areas, such as Biomedical, Energy and Environmental Sciences and New information and Communication Technologies, with very interesting properties, and whose innovative applications can have an outstanding impact in our world. This dissertation is a proposal of contextualization and experimentation of theme "Electrochromic Materials and Electrochemical Artificial Muscles" in the Program of Chemistry, in 12th year, particularly in the third unit - Plastics, Glass and New Materials. lt will present a sub-unit that can integrate the unit above. The definitions and some applications of the Electrochromic Materials and Electrochemical Artificial Muscles are showed. Demonstrative and illustrative laboratory experiments of the theme are proposed. ln order to support the teaching of that subject, it is presented an animated graphical presentation in Power Point.

Design of experiment in drug development: optimal allocations in multi-arm clinical trials and design space for multi-step processes

In this thesis, we deal with the design of experiments in the drug development process, focusing on the design of clinical trials for treatment comparisons (Part I) and the design of preclinical laboratory experiments for proteins development and manufacturing (Part II). In Part I we propose a multi-purpose design methodology for sequential clinical trials. We derived optimal allocations of patients to treatments for testing the efficacy of several experimental groups by also taking into account ethical considerations. We first consider exponential responses for survival trials and we then present a unified framework for heteroscedastic experimental groups that encompasses the general ANOVA set-up. The very good performance of the suggested optimal allocations, in terms of both inferential and ethical characteristics, are illustrated analytically and through several numerical examples, also performing comparisons with other designs proposed in the literature. Part II concerns the planning of experiments for processes composed of multiple steps in the context of preclinical drug development and manufacturing. Following the Quality by Design paradigm, the objective of the multi-step design strategy is the definition of the manufacturing design space of the whole process and, as we consider the interactions among the subsequent steps, our proposal ensures the quality and the safety of the final product, by enabling more flexibility and process robustness in the manufacturing.

A methodological approach for the performance optimization of transient seepage models through inverse analysis

The topic of the Ph.D project focuses on the modelling of the soil-water dynamics inside an instrumented embankment section along Secchia River (Cavezzo (MO)) in the period from 2017 to 2018 and the quantification of the performance of the direct and indirect simulations . The commercial code Hydrus2D by Pc-Progress has been chosen to run the direct simulations. Different soil-hydraulic models have been adopted and compared. The parameters of the different hydraulic models are calibrated using a local optimization method based on the Levenberg - Marquardt algorithm implemented in the Hydrus package. The calibration program is carried out using different types of dataset of observation points, different weighting distributions, different combinations of optimized parameters and different initial sets of parameters. The final goal is an in-depth study of the potentialities and limits of the inverse analysis when applied to a complex geotechnical problem as the case study. The second part of the research focuses on the effects of plant roots and soil-vegetation-atmosphere interaction on the spatial and temporal distribution of pore water pressure in soil. The investigated soil belongs to the West Charlestown Bypass embankment, Newcastle, Australia, that showed in the past years shallow instabilities and the use of long stem planting is intended to stabilize the slope. The chosen plant species is the Malaleuca Styphelioides, native of eastern Australia. The research activity included the design and realization of a specific large scale apparatus for laboratory experiments. Local suction measurements at certain intervals of depth and radial distances from the root bulb are recorded within the vegetated soil mass under controlled boundary conditions. The experiments are then reproduced numerically using the commercial code Hydrus 2D. Laboratory data are used to calibrate the RWU parameters and the parameters of the hydraulic model.

Eruptive and depositional processes of widely dispersed volcanic ash: Insights from the Brown Tuffs (Aeolian Islands)

This is a multidisciplinary study of the Brown Tuffs (BT) ash deposits of the Aeolian Islands in northern Sicily and representing the most voluminous and widely distributed tephra deposit in this region. A large dataset of major and minor elements of the BT glass has defined a range from K-series basaltic-andesites and trachy-andesites through to tephri-phonolites and trachytes that is consistent with the Vulcano magmatic system. Combined with stratigraphic information and new radiocarbon ages, four stratigraphic macro-units are defined: the Lower (80-56 ky; LBT), Intermediate (56-27 ky; IBT), Intermediate-upper (26-24 ky; IBT-upper) and Upper BT (24-6 ky; UBT). Glass compositional data provide constraints on proximal-distal correlations of the BT with deep-sea tephra layers in the Tyrrhenian and Adriatic Seas and new insights on the definition of the dispersal area of the BT eruptions. Sedimentological evidence of massive to stratified deposits and shear-related structures, coupled with grain-size and componentry analyses, have allowed to interpret the BT as the result of laterally-spreading, concentrated ash-rich PDCs, with a high potential of erosion of the substratum. Shear-structures similar to those observed in the field in the BT deposits have been reproduced by small-medium scale laboratory experiments carried out on ash granular flows, which have also allowed to describe the behaviour of ash-rich PDcs and their mobility depending on variations of slope-ratio, grain size and flow channelization. The resulting integrated dataset provides a contribution to the knowledge of the BT eruptions and insights on long-term hazard assessment in the study area. The eruptive dynamics of the BT may have a role in characterizing the whole magmatic system of the La Fossa Caldera on Vulcano, in the light of the geochemical link highlighted between the UBT macrounit and the early products of the La Fossa cone.

CFD Applications for Wave Energy Conversion Devices (MoonWEC) and Turbulent Fountains for Environmental Fluid Mechanics

This thesis is based on two studies that are related to floating wave energy conversion (WEC) devices and turbulent fountains. The ability of the open-source CFD software OpenFOAM® has been studied to simulate these phenomena. The CFD model has been compared with the physical experimental results. The first study presents a model of a WEC device, called MoonWEC, which is patented by the University of Bologna. The CFD model of the MoonWEC under the action of waves has been simulated using OpenFOAM and the results are promising. The reliability of the CFD model is confirmed by the laboratory experiments, conducted at the University of Bologna, for which a small-scale prototype of the MoonWEC was made from wood and brass. The second part of the thesis is related to the turbulent fountains which are formed when a heavier source fluid is injected upward into a lighter ambient fluid, or else a lighter source fluid is injected downward into a heavier ambient fluid. For this study, the first case is considered for laboratory experiments and the corresponding CFD model. The vertical releases of the source fluids into a quiescent, uniform ambient fluid, from a circular source, were studied with different densities in the laboratory experiments, conducted at the University of Parma. The CFD model has been set up for these experiments. Favourable results have been observed from the OpenFOAM simulations for the turbulent fountains as well, indicating that it can be a reliable tool for the simulation of such phenomena.

Fatigue Design Challenges of Transition Pieces from Decommissioned Platforms for Offshore Wind Energy

All structures are subjected to various loading conditions and combinations. For offshore structures, these loads include permanent loads, hydrostatic pressure, wave, current, and wind loads. Typically, sea environments in different geographical regions are characterized by the 100-year wave height, surface currents, and velocity speeds. The main problems associated with the commonly used, deterministic method is the fact that not all waves have the same period, and that the actual stochastic nature of the marine environment is not taken into account. Offshore steel structure fatigue design is done using the DNVGL-RP-0005:2016 standard which takes precedence over the DNV-RP-C203 standard (2012). Fatigue analysis is necessary for oil and gas producing offshore steel structures which were first constructed in the Gulf of Mexico North Sea (the 1930s) and later in the North Sea (1960s). Fatigue strength is commonly described by S-N curves which have been obtained by laboratory experiments. The rapid development of the Offshore wind industry has caused the exploration into deeper ocean areas and the adoption of new support structural concepts such as full lattice tower systems amongst others. The optimal design of offshore wind support structures including foundation, turbine towers, and transition piece components putting into consideration, economy, safety, and even the environment is a critical challenge. In this study, fatigue design challenges of transition pieces from decommissioned platforms for offshore wind energy are proposed to be discussed. The fatigue resistance of the material and structural components under uniaxial and multiaxial loading is introduced with the new fatigue design rules whilst considering the combination of global and local modeling using finite element analysis software programs.

Seasonal fluctuations of bacterial community diversity in agricultural soil and experimental validation by laboratory disturbance experiments.

Natural fluctuations in soil microbial communities are poorly documented because of the inherent difficulty to perform a simultaneous analysis of the relative abundances of multiple populations over a long time period. Yet, it is important to understand the magnitudes of community composition variability as a function of natural influences (e.g., temperature, plant growth, or rainfall) because this forms the reference or baseline against which external disturbances (e.g., anthropogenic emissions) can be judged. Second, definition of baseline fluctuations in complex microbial communities may help to understand at which point the systems become unbalanced and cannot return to their original composition. In this paper, we examined the seasonal fluctuations in the bacterial community of an agricultural soil used for regular plant crop production by using terminal restriction fragment length polymorphism profiling (T-RFLP) of the amplified 16S ribosomal ribonucleic acid (rRNA) gene diversity. Cluster and statistical analysis of T-RFLP data showed that soil bacterial communities fluctuated very little during the seasons (similarity indices between 0.835 and 0.997) with insignificant variations in 16S rRNA gene richness and diversity indices. Despite overall insignificant fluctuations, between 8 and 30% of all terminal restriction fragments changed their relative intensity in a significant manner among consecutive time samples. To determine the magnitude of community variations induced by external factors, soil samples were subjected to either inoculation with a pure bacterial culture, addition of the herbicide mecoprop, or addition of nutrients. All treatments resulted in statistically measurable changes of T-RFLP profiles of the communities. Addition of nutrients or bacteria plus mecoprop resulted in bacteria composition, which did not return to the original profile within 14 days. We propose that at less than 70% similarity in T-RFLP, the bacterial communities risk to drift apart to inherently different states.

Laboratory shearing experiments at low sliding velocities of IODP Hole 343-C0019E

The 2011 Tohoku-Oki earthquake demonstrated that the shallowest reaches of plate boundary subduction megathrusts can host substantial coseismic slip that generates large and destructive tsunamis, contrary to the common assumption that the frictional properties of unconsolidated clay-rich sediments at depths less than View the MathML source should inhibit rupture. We report on laboratory shearing experiments at low sliding velocities (View the MathML source) using borehole samples recovered during IODP Expedition 343 (JFAST), spanning the plate-boundary décollement within the region of large coseismic slip during the Tohoku earthquake. We show that at sub-seismic slip rates the fault is weak (sliding friction µs=0.2-0.26), in contrast to the much stronger wall rocks (µs>~0.5). The fault is weak due to elevated smectite clay content and is frictionally similar to a pelagic clay layer of similar composition. The higher cohesion of intact wall rock samples coupled with their higher amorphous silica content suggests that the wall rock is stronger due to diagenetic cementation and low clay content. Our measurements also show that the strongly developed in-situ fabric in the fault zone does not contribute to its frictional weakness, but does lead to a near-cohesionless fault zone, which may facilitate rupture propagation by reducing shear strength and surface energy at the tip of the rupture front. We suggest that the shallow rupture and large coseismic slip during the 2011 Tohoku earthquake was facilitated by a weak and cohesionless fault combined with strong wall rocks that drive localized deformation within a narrow zone.

... Heat transmission through walls, concretes, and plasters; experiments carried out at the National physical laboratory /

"Investigations relating to the heat-insulating efficiency of building construction, by H. Kreüger and A. Eriksson": p. 28-31.