729 resultados para Keating, Jenny


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In the present study, allele frequency distributions for the 15 STR loci included in the PowerPlex® 16 Systems (Promega) were obtained from a sample of 55 unrelated individuals living in Araraquara region (SP, Brazil). The frequency of each allele for each locus tested, the exact test and the forensic and paternity parameters were calculated using POWERSTATS ver. 1.2 (Promega) and GENEPOP ver. 3.2 software. All loci are in the Hardy-Weinberg equilibrium and they reached a combined power discrimination of 0.999999999999999973 and combined power exclusion of 0.99999987, showing to be a powerful tool for paternity testing and individual identification in the population analyzed. © 2005 Elsevier B.V. All rights reserved.

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Includes bibliography

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Incluye Bibliografía

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Pós-graduação em Letras - FCLAS

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Estudos Literários - FCLAR

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Pós-graduação em Letras - FCLAS

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Propolis effect on the growth and apoptosis of human lung adenocarcinoma (A549 cells) was investigated as well as its mechanisms. Cells were incubated with propolis for 72 h, and 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were employed to assess cell viability and the inhibitory concentration (IC). Apoptosis was detected by Acridine Orange/Ethidium Bromide and 4',6-diamidino-2-phenylindole staining after 24 and 48 h of incubation with ¼ IC50 of propolis by testing the mitochondrial membrane potential (ΔΨm) and the expression of apoptosis-related genes (p53, Caspase-3, Bax, Bcl-2, Bcl-XL , Noxa, Puma and p21) by reverse transcription polymerase chain reaction. Propolis displayed antiproliferative and cytotoxic effects on A549 cells in a dose- and time-dependent manner, but it did not suppress the growth of normal Vero cells. An enhanced apoptosis was seen in A549 propolis-treated cells after 48 h compared with the control cells. Propolis decreased mitochondrial membrane potential by overexpression of pro-apoptotic genes (Bax and Noxa) and reduction of the antiapoptotic gene Bcl-XL . The expression level of other genes remained unchanged (p53, Caspse-3 and Bax), whereas p21 expression was increased. Propolis induced caspase-independent apoptosis through a p53-independent mitochondrial pathway, and cell cycle arrest by upregulation of p21. Although propolis induces apoptosis mainly by p53-independent manner, it may be induced by another pathway, and new insights may arise for preventing or treating lung cancer.

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Abstract The goal of this study was to conduct a comparative analysis of three university recycling programs. This study looked at several aspects of the programs that included the diversion rates, per capita ratios of materials recycled and disposed, and the average net costs of waste disposal and waste diversion. The universities included in this study were the University of Nebraska-Lincoln, the University of Colorado at Boulder, and the University of Oregon. To gather the information necessary for this analysis, I contacted each of the university’s recycling coordinators. To determine the average net costs of waste disposal and waste diversion I requested both the recycling budget and solid waste budget from each university for the fiscal years of interest which included: 2006-2007, 2007-2008, and 2008-2009. To calculate the diversion rates and per capita ratios, I requested performance records from each university listing the tonnage of materials recycled and disposed for the same years. This study’s findings reported that the average net costs for waste diversion in all three universities were $22-$122 less per ton than costs for waste collection and disposal. This study also indicated that the universities with the highest diversion and recycling rates were the University of Colorado at Boulder and the University of Oregon. The university with the lowest waste generated per capita was the University of Oregon followed by the University of Nebraska-Lincoln.