cDNA cloning and expression of a novel cell surface-expressed \b heparan sulfate/heparin \b interacting \b protein (HIP) from human cell lines and functional studies of a synthetic HIP peptide

Heparan sulfate proteoglycans and their corresponding binding sites have been suggested to play an important role during the initial attachment of blastocysts to uterine epithelium and human trophoblastic cell lines to uterine epithelial cell lines. Previous studies on RL95 cells, a human uterine epithelial cell line, characterized a single class of cell surface heparin/heparan sulfate (HP/HS)-binding sites. Three major HP/HS-binding peptide fragments were isolated from RL95 cell surfaces by tryptic digestion and partial amino-terminal amino acid sequence from each peptide fragment was obtained. In the current study, using the approaches of reverse transcription-polymerase chain reaction and cDNA library screening, a novel cell surface $\rm\underline{H}$P/HS $\rm\underline{i}$nteracting $\rm\underline{p}$rotein (HIP) has been isolated from RL95 cells. The full-length cDNA of HIP encodes a protein of 259 amino acids with a calculated molecular weight of 17,754 Da and pI of 11.75. Transfection of HIP cDNA into NIH-3T3 cells demonstrated cell surface expression and a size similar to that of HIP expressed by human cells. Predicted amino acid sequence indicates that HIP lacks a membrane spanning region and has no consensus sites for glycosylation. Northern blot analysis detected a single transcript of 1.3 kb in both total RNA and poly(A$\sp+$) RNA. Examination of human cell lines and normal tissues using both Northern blot and Western blot analysis revealed that HIP is differentially expressed in a variety of human cell lines and normal tissues, but absent in some cell lines examined. HIP has about 80% homology, at the level of both mRNA and protein, to a rodent protein, designated as ribosomal protein L29. Thus, members of the L29 family may be displayed on cell surfaces where they participate in HP/HS binding events. Studies on a synthetic peptide derived from HIP demonstrate that HIP peptide binds HS/HP with high selectivity and has high affinity (Kd = 10 nM) for a subset of polysaccharides found in commercial HIP preparations. Moreover, HIP peptide also binds certain forms of cell surface, but not secreted or intracellular. HS expressed by RL95 and JAR cells. This peptide supports the attachment of several human trophoblastic cell lines and a variety of mammalian adherent cell lines in a HS-dependent fashion. Furthermore, studies on the subset of HP specifically recognized by HIP peptide indicate that this high-affinity HP (HA-HP) has a larger median MW and a greater negative charge density than bulk HP. The minimum size of oligosaccharide required to bind to HIP peptide with high affinity is a septa- or octasaccharide. HA-HP also quantitatively binds to antithrombin-III (AT-III) with high affinity, indicating that HIP peptide and AT-III may recognize the same or similar oligosaccharide structure(s). Furthermore, HIP peptide antagonizes HP action and promotes blood coagulation in both factor Xa- and thrombin-dependent assays. Finally, HA-HP recognized by HP peptide is highly enriched with anticoagulant activity relative to bulk HP. Collectively, these results demonstrate that HIP may play a role in the HP/HS-involved cell-cell and cell-matrix interactions and recognizes a motif in HP similar or identical to that recognized by AT-III and therefore, may modulate blood coagulation. ^

Cancer risk and use of protease inhibitor or nonnucleoside reverse transcriptase inhibitor-based combination antiretroviral therapy: the d: a: d study.

BACKGROUND The association between combination antiretroviral therapy (cART) and cancer risk, especially regimens containing protease inhibitors (PIs) or nonnucleoside reverse transcriptase inhibitors (NNRTIs), is unclear. METHODS Participants were followed from the latest of D:A:D study entry or January 1, 2004, until the earliest of a first cancer diagnosis, February 1, 2012, death, or 6 months after the last visit. Multivariable Poisson regression models assessed associations between cumulative (per year) use of either any cART or PI/NNRTI, and the incidence of any cancer, non-AIDS-defining cancers (NADC), AIDS-defining cancers (ADC), and the most frequently occurring ADC (Kaposi sarcoma, non-Hodgkin lymphoma) and NADC (lung, invasive anal, head/neck cancers, and Hodgkin lymphoma). RESULTS A total of 41,762 persons contributed 241,556 person-years (PY). A total of 1832 cancers were diagnosed [incidence rate: 0.76/100 PY (95% confidence interval: 0.72 to 0.79)], 718 ADC [0.30/100 PY (0.28-0.32)], and 1114 NADC [0.46/100 PY (0.43-0.49)]. Longer exposure to cART was associated with a lower ADC risk [adjusted rate ratio: 0.88/year (0.85-0.92)] but a higher NADC risk [1.02/year (1.00-1.03)]. Both PI and NNRTI use were associated with a lower ADC risk [PI: 0.96/year (0.92-1.00); NNRTI: 0.86/year (0.81-0.91)]. PI use was associated with a higher NADC risk [1.03/year (1.01-1.05)]. Although this was largely driven by an association with anal cancer [1.08/year (1.04-1.13)], the association remained after excluding anal cancers from the end point [1.02/year (1.01-1.04)]. No association was seen between NNRTI use and NADC [1.00/year (0.98-1.02)]. CONCLUSIONS Cumulative use of PIs may be associated with a higher risk of anal cancer and possibly other NADC. Further investigation of biological mechanisms is warranted.

Norm and Anomaly in the Jewish Life of Jesus (Toledot Yeshu)

The Toledoth Yeshu, the “Generation,” or “Life of Jesus,” have been described as an anti-Gospel, or a parody of the Gospel. This protean tradition, witnessed in more than hundred manuscripts and printed editions, offers a “counter-history” of the life of Jesus and the origins of Christianity. According to this mischievous narrative Jesus was an illegitimate child turned charlatan, and his disciples a bunch of violent and senseless rogues who continued to stir up trouble in Israel even following their leader’s shameful hanging. The Toledoth Yeshu is the story of an anomaly (Jesus and the birth of Christianity). It is also a story about confusion: marital confusion, social confusion, and religious confusion. As an exercise in “historical imagination,” the Toledoth Yeshu offers a narrative of religions compared, and a reflection on social and religious borders, on their instability and fragility, and ultimately on their necessity. The present paper will explore the normative dimension of the Toledoth Yeshu tradition: the way the “disorder of things” the narrative relates also conveys a powerful discourse on social and religious norms. We will also seek to map this tradition in the broader context of medieval Jewish discussions on Jesus (particularly Maimonides) as a “case” in the religious history of mankind, addressing issues of false prophecy, religious deviation, transgression, and heresy.

Visibilidad de los archivos universitarios hispanoamericanos a través de Internet

Este trabajo busca detectar la presencia en Internet de archivos universitarios existentes en países del continente americano cuyo idioma oficial sea la lengua española, y analizar sus sitios web prestando especial atención a los contenidos difundidos en ellos. Se relevaron a junio de 2013 un total de 56 sitios web. Como conclusiones principales pueden señalarse las siguientes: a) se observa un lento incremento en la cantidad de sitios web, pasando de 42 en 2005 a 56 en 2013; b) 52 por ciento corresponden a la fase histórica; c) México es el país con mayor cantidad de archivos; d) 64 por ciento posee sitio web propio; e) 80 por ciento incluye misión del archivo; f) cerca de un 20 por ciento no incluye datos de contacto; g) 74 por ciento señala los servicios ofrecidos; h) 53 por ciento disponen información descriptiva general sobre los fondos custodiados; i) 21 por ciento ofrece la posibilidad de hojear documentos a texto completo; j) 28 por ciento difunde su reglamento y procedimientos de trabajo; k) poco más del 45 por ciento incluye información sobre pautas para el acceso, consulta y reproducción de documentos; l) un bajo porcentaje incluye informaciones adicionales, predominando noticias (39 por ciento) y fotos (18 por ciento), así como legislación archivística (20 por ciento) y recursos archivísticos de interés (27 por ciento); m) en cuanto a las herramientas de la Web 2.0, 18 por ciento posee blog, 16 por ciento canales de RSS, 27 por ciento Facebook y 23 por ciento Twitter

Crises of social significances: adolescents and their projects for future work

This is a report on data raised through research, which has already finished, about Identification references in adolescents . Objetives : To analyze and reflect upon the collected data, adolescents' representations about the work field and their projects for future work. To conceptualize about the crises of social significances which produce and order sense and, therefore, organize our perceptions of work through social discourses and practices which have an impact on the subjectivity of adolescents and young adults. Methodology: Brief presentation of the concepts which frame the qualitative research in this area. Reading and analysis of fragments from adolescents' accounts, obtained in focal groups at different state secondary schools. Conclusions: The adolescents researched portray a self-image full of uncertainty and skepticism as regards their future possibilities. The impact produced by the fall of work as an ideal of doing and being is made evident in their accounts. Only some of them link their future project with an invested and valuable activity, even if this is not associated with economic safety and wellbeing. Others present themselves as incapable to modify the state of things, which offers a field connected with consumerist values and with the idea that "you are worth what you have (in material things)".

Visibilidad de los archivos universitarios hispanoamericanos a través de Internet

Este trabajo busca detectar la presencia en Internet de archivos universitarios existentes en países del continente americano cuyo idioma oficial sea la lengua española, y analizar sus sitios web prestando especial atención a los contenidos difundidos en ellos. Se relevaron a junio de 2013 un total de 56 sitios web. Como conclusiones principales pueden señalarse las siguientes: a) se observa un lento incremento en la cantidad de sitios web, pasando de 42 en 2005 a 56 en 2013; b) 52 por ciento corresponden a la fase histórica; c) México es el país con mayor cantidad de archivos; d) 64 por ciento posee sitio web propio; e) 80 por ciento incluye misión del archivo; f) cerca de un 20 por ciento no incluye datos de contacto; g) 74 por ciento señala los servicios ofrecidos; h) 53 por ciento disponen información descriptiva general sobre los fondos custodiados; i) 21 por ciento ofrece la posibilidad de hojear documentos a texto completo; j) 28 por ciento difunde su reglamento y procedimientos de trabajo; k) poco más del 45 por ciento incluye información sobre pautas para el acceso, consulta y reproducción de documentos; l) un bajo porcentaje incluye informaciones adicionales, predominando noticias (39 por ciento) y fotos (18 por ciento), así como legislación archivística (20 por ciento) y recursos archivísticos de interés (27 por ciento); m) en cuanto a las herramientas de la Web 2.0, 18 por ciento posee blog, 16 por ciento canales de RSS, 27 por ciento Facebook y 23 por ciento Twitter

Crises of social significances: adolescents and their projects for future work

This is a report on data raised through research, which has already finished, about Identification references in adolescents . Objetives : To analyze and reflect upon the collected data, adolescents' representations about the work field and their projects for future work. To conceptualize about the crises of social significances which produce and order sense and, therefore, organize our perceptions of work through social discourses and practices which have an impact on the subjectivity of adolescents and young adults. Methodology: Brief presentation of the concepts which frame the qualitative research in this area. Reading and analysis of fragments from adolescents' accounts, obtained in focal groups at different state secondary schools. Conclusions: The adolescents researched portray a self-image full of uncertainty and skepticism as regards their future possibilities. The impact produced by the fall of work as an ideal of doing and being is made evident in their accounts. Only some of them link their future project with an invested and valuable activity, even if this is not associated with economic safety and wellbeing. Others present themselves as incapable to modify the state of things, which offers a field connected with consumerist values and with the idea that "you are worth what you have (in material things)".

Crises of social significances: adolescents and their projects for future work

This is a report on data raised through research, which has already finished, about Identification references in adolescents . Objetives : To analyze and reflect upon the collected data, adolescents' representations about the work field and their projects for future work. To conceptualize about the crises of social significances which produce and order sense and, therefore, organize our perceptions of work through social discourses and practices which have an impact on the subjectivity of adolescents and young adults. Methodology: Brief presentation of the concepts which frame the qualitative research in this area. Reading and analysis of fragments from adolescents' accounts, obtained in focal groups at different state secondary schools. Conclusions: The adolescents researched portray a self-image full of uncertainty and skepticism as regards their future possibilities. The impact produced by the fall of work as an ideal of doing and being is made evident in their accounts. Only some of them link their future project with an invested and valuable activity, even if this is not associated with economic safety and wellbeing. Others present themselves as incapable to modify the state of things, which offers a field connected with consumerist values and with the idea that "you are worth what you have (in material things)".

Visibilidad de los archivos universitarios hispanoamericanos a través de Internet

Este trabajo busca detectar la presencia en Internet de archivos universitarios existentes en países del continente americano cuyo idioma oficial sea la lengua española, y analizar sus sitios web prestando especial atención a los contenidos difundidos en ellos. Se relevaron a junio de 2013 un total de 56 sitios web. Como conclusiones principales pueden señalarse las siguientes: a) se observa un lento incremento en la cantidad de sitios web, pasando de 42 en 2005 a 56 en 2013; b) 52 por ciento corresponden a la fase histórica; c) México es el país con mayor cantidad de archivos; d) 64 por ciento posee sitio web propio; e) 80 por ciento incluye misión del archivo; f) cerca de un 20 por ciento no incluye datos de contacto; g) 74 por ciento señala los servicios ofrecidos; h) 53 por ciento disponen información descriptiva general sobre los fondos custodiados; i) 21 por ciento ofrece la posibilidad de hojear documentos a texto completo; j) 28 por ciento difunde su reglamento y procedimientos de trabajo; k) poco más del 45 por ciento incluye información sobre pautas para el acceso, consulta y reproducción de documentos; l) un bajo porcentaje incluye informaciones adicionales, predominando noticias (39 por ciento) y fotos (18 por ciento), así como legislación archivística (20 por ciento) y recursos archivísticos de interés (27 por ciento); m) en cuanto a las herramientas de la Web 2.0, 18 por ciento posee blog, 16 por ciento canales de RSS, 27 por ciento Facebook y 23 por ciento Twitter

Exploring the logic of mobile search

After more than a decade of development work and hopes, the usage of mobile Internet has finally taken off. Now, we are witnessing the first signs of evidence of what might become the explosion of mobile content and applications that will be shaping the (mobile) Internet of the future. Similar to the wired Internet, search will become very relevant for the usage of mobile Internet. Current research on mobile search has applied a limited set of methodologies and has also generated a narrow outcome of meaningful results. This article covers new ground, exploring the use and visions of mobile search with a users' interview-based qualitative study. Its main conclusion builds upon the hypothesis that mobile search is sensitive to a mobile logic different than today's one. First, (advanced) users ask for accessing with their mobile devices the entire Internet, rather than subsections of it. Second, success is based on new added-value applications that exploit unique mobile functionalities. The authors interpret that such mobile logic involves fundamentally the use of personalised and context-based services.

Sobre la identidad de la ciudad. El estudio de la interacción audiovisual en las nuevas herramientas para el proyecto. El caso de Cuenca

Esta investigación se centra en el estudio de la dimensión audiovisual de la arquitectura, como aproximación intersensorial a la aprehensión e ideación del espacio. Poniendo en evidencia la complejidad de la relación hombre-medio, se plantea la necesidad de desarrollar nuevas metodologías y herramientas que tengan en cuenta dicha complejidad y que favorezcan el desarrollo del proyecto. Nos mueve en esta investigación la convicción de que los cambios rápidos y profundos que caracterizan nuestros tiempos en todos los ámbitos, social, económico, político… entrañan inevita-blemente nuevos modos de conocimiento y experimentación del espacio, y por tanto nuevos ejes de investigación. La creciente valoración, en todos los campos del conocimiento, de los aspectos subjetivos y sensoriales, el desarrollo de las tecnologías que ha cambiado completamente nuestras relaciones interpersonales y con el entorno, las nuevas capacidades de análisis, grabación y conservación y manipulación de datos y por ultimo, aunque no menos importante, la puesta a disposición democrá¬tica y global de todo el saber a través de Internet, imponen otra aproximación al hacer, concebir y vivir la arquitectura. Esta investigación se centra en un análisis crítico del estado de la cuestión, construyendo nue¬vas redes de relación entre disciplinas, que permitan plantear la dimensión audiovisual como un nuevo eje de investigación dentro de la arquitectura, poniendo en evidencia la necesidad de desa¬rrollar análisis de forma trasversal e interdisciplinar. Hemos prestado particular atención a la evolución de lo sonoro y su aproximación cualitativa a la arquitectura, mostrando como el sonido, con su capacidad de introducir el tiempo y los aspectos dinámicos (el movimiento, la presencia del cuerpo…), no es simplemente otro canal sensorial en la aprehensión del espacio, ya que su interacción con lo visual genera un espacio-tiempo indisociable, propio, característico de cada momento y lugar. A partir de este planteamiento se ha hecho una revisión metodológica dirigida a utilizar el reco¬rrido como herramienta de análisis, que permita estudiar la relación entre el espacio, la acción y la percepción audio-visual, cruzando para ello los datos correspondientes a la morfología del espacio, con los datos de la experiencia perceptiva individual y con los de los usos colectivos del espacio, utilizándose finalmente el video como un herramienta, no sólo de representación de lo real, sino también como instrumento de análisis, que permite tomar datos (grabaciones audio, video, obser¬vaciones…), aislarlos, estudiarlos, clasificarlos, ordenarlos, y finalmente, restituirlos mediante el montaje. Se ha realizado una primera experimentación “in situ” que ha servido para explorar la aplicación del método, planteando nuevas preguntas y abriendo líneas de análisis para ulteriores investigacio¬nes. ABSTRACT This research is focused on the study of the audiovisual dimension of architecture, as an in¬tersensorial approach to space apprehension and design. It is posed the necessity to develop new methodologies and tools that keep this complexity, as a contribution to the development of a project, by means of putting into evidence the sophistication of the relationship between man and media The research moves us to the conviction that the quick and relevant changes that confer a distinc-tion to these contemporary times all over the social, economic and political environments, involve, unavoidably, new ways of knowledge and experimentation on space, and therefore, new trends of research. The growing valuation of subjective and sensorial aspects all over the fields of the knowledge and the development of the technologies that have changed completely our interpersonal and environmental relationships, the new tools for analysis, recording, conservation and manipulation of data and, last but not least, the setting to democratic and global availability of the whole knowledge through Inter¬net, impose another approach to the making, conception and experience of architecture. This research deals with a critical analysis of the state–of- the-art of the matter, modelling new webs of relationship among disciplines that allow to outline the audiovisual dimension as a new focus of research on architecture, putting evidence into practice as it is necessary to develop any analysis in a transversal and interdisciplinary way. It is paid a special attention to the evolution of sound objects and their qualitative approach to ar¬chitecture, showing how sound, with its capacity to transmit time and dynamic aspects of things (movement, the presence of the body), it is not simply another sensorial channel in the apprehension of space, since its interaction with the visual thing generates an undetachable association of space and time, an specific one of every moment and place. Starting from this position a methodological revision has been made leading to use a walk as a tool for analysis that allows to study the relationship among the space, the action and the audio-visual perception, by means of crossing data corresponding to the morphology of space, with the data of a perceptive experience from the perspective of an individual observer and with those of the collective uses of the space, as video has been finally used as a tool, not only as a representation of the real thing, but also as a tool for analysis that allows to take isolated data (audio recordings, video, obser¬vations), to be studied, classified, and put into their appropriate place, and finally, to restore them by means of a multimedia set up. A first experimentation in situ has been carried out, being useful to explore a method of appli¬cation, outlining new questions and beginning with new ways of analysis for further research.

Gestión de la información en redes de sensores inalámbricos

La gestión del conocimiento (KM) es el proceso de recolectar datos en bruto para su análisis y filtrado, con la finalidad de obtener conocimiento útil a partir de dichos datos. En este proyecto se pretende hacer un estudio sobre la gestión de la información en las redes de sensores inalámbricos como inicio para sentar las bases para la gestión del conocimiento en las mismas. Las redes de sensores inalámbricos (WSN) son redes compuestas por sensores (también conocidos como motas) distribuidos sobre un área, cuya misión es monitorizar una o varias condiciones físicas del entorno. Las redes de sensores inalámbricos se caracterizan por tener restricciones de consumo para los sensores que utilizan baterías, por su capacidad para adaptarse a cambios y ser escalables, y también por su habilidad para hacer frente a fallos en los sensores. En este proyecto se hace un estudio sobre la gestión de la información en redes de sensores inalámbricos. Se comienza introduciendo algunos conceptos básicos: arquitectura, pila de protocolos, topologías de red, etc.… Después de esto, se ha enfocado el estudio hacia TinyDB, el cual puede ser considerado como parte de las tecnologías más avanzadas en el estado del arte de la gestión de la información en redes de sensores inalámbricos. TinyDB es un sistema de procesamiento de consultas para extraer información de una red de sensores. Proporciona una interfaz similar a SQL y permite trabajar con consultas contra la red de sensores inalámbricos como si se tratara de una base de datos tradicional. Además, TinyDB implementa varias optimizaciones para manejar los datos eficientemente. En este proyecto se describe también la implementación de una sencilla aplicación basada en redes de sensores inalámbricos. Las motas en la aplicación son capaces de medir la corriente a través de un cable. El objetivo de esta aplicación es monitorizar el consumo de energía en diferentes zonas de un área industrial o doméstico, utilizando redes de sensores inalámbricas. Además, se han implementado las optimizaciones más importantes que se han aprendido en el análisis de la plataforma TinyDB. Para desarrollar esta aplicación se ha utilizado como sensores la plataforma open-source de creación de prototipos electrónicos Arduino, y el ordenador de placa reducida Raspberry Pi como coordinador. ABSTRACT. Knowledge management (KM) is the process of collecting raw data for analysis and filtering, to get a useful knowledge from this data. In this project the information management in wireless sensor networks is studied as starting point before knowledge management. Wireless sensor networks (WSN) are networks which consists of sensors (also known as motes) distributed over an area, to monitor some physical conditions of the environment. Wireless sensor networks are characterized by power consumption constrains for sensors which are using batteries, by the ability to be adaptable to changes and to be scalable, and by the ability to cope sensor failures. In this project it is studied information management in wireless sensor networks. The document starts introducing basic concepts: architecture, stack of protocols, network topology… After this, the study has been focused on TinyDB, which can be considered as part of the most advanced technologies in the state of the art of information management in wireless sensor networks. TinyDB is a query processing system for extracting information from a network of sensors. It provides a SQL-like interface and it lets us to work with queries against the wireless sensor network like if it was a traditional database. In addition, TinyDB implements a lot of optimizations to manage data efficiently. In this project, it is implemented a simple wireless sensor network application too. Application’s motes are able to measure amperage through a cable. The target of the application is, by using a wireless sensor network and these sensors, to monitor energy consumption in different areas of a house. Additionally, it is implemented the most important optimizations that we have learned from the analysis of TinyDB platform. To develop this application it is used Arduino open-source electronics prototyping platform as motes, and Raspberry Pi single-board computer as coordinator.

Design, development and evaluation of a portable recording system to capture dynamic presentations using the teacher's tablet PC

Computers and multimedia equipment have improved a lot in the last years. They have reduced their cost and size while at the same time increased their capabilities. These improvements allowed us to design and implement a portable recording system that also integrates the teacher´s tablet PC to capture what he/she writes on the slides and all that happens in it. This paper explains this system in detail and the validation of the recordings that we did after using it to record all the lectures the “Communications Software” course in our university. The results show that pupils used the recordings for different purposes and consider them useful for a variety of things, especially after missing a lecture.