Host genome influences on susceptibility to HIV-1

In vitro and in vivo analyses identified a significant component of heritability in cellular or host susceptibility to HIV-1. The bases for susceptibility can be traced to genetic differences (inter-species) resulting from evolutionary adaptation to exogenous (and endogenous) retroviral infections, and to intra-species and inter-individual (human) differences associated with genetic variation. We have completed large scale evolutionary analysis of genes involved in HIV life cycle and pathogenesis, as well as participating and conducting genome-wide association studies, linkage analysis, and transcriptome analysis. These studies allowed a better understanding of the influence of common human variants in HIV-1 susceptibility and define a number of experimental challenges in the filed: understanding of the role of rare and private mutations in susceptibility, and the development of better tools for the integration of data from large-scale studies.

Defining the RNA polymerase III transcriptome: Genome-wide localization of the RNA polymerase III transcription machinery in human cells.

Our view of the RNA polymerase III (Pol III) transcription machinery in mammalian cells arises mostly from studies of the RN5S (5S) gene, the Ad2 VAI gene, and the RNU6 (U6) gene, as paradigms for genes with type 1, 2, and 3 promoters. Recruitment of Pol III onto these genes requires prior binding of well-characterized transcription factors. Technical limitations in dealing with repeated genomic units, typically found at mammalian Pol III genes, have so far hampered genome-wide studies of the Pol III transcription machinery and transcriptome. We have localized, genome-wide, Pol III and some of its transcription factors. Our results reveal broad usage of the known Pol III transcription machinery and define a minimal Pol III transcriptome in dividing IMR90hTert fibroblasts. This transcriptome consists of some 500 actively transcribed genes including a few dozen candidate novel genes, of which we confirmed nine as Pol III transcription units by additional methods. It does not contain any of the microRNA genes previously described as transcribed by Pol III, but reveals two other microRNA genes, MIR886 (hsa-mir-886) and MIR1975 (RNY5, hY5, hsa-mir-1975), which are genuine Pol III transcription units.

Condition of the State of Iowa, 1888 (22nd General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Larrabee delivered at the State Capitol.

Condition of the State of Iowa, 1902 (29nd General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Shaw delivered at the State Capitol.

Condition of the State of Iowa, 1904 (30th General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Cummins delivered at the State Capitol.

Condition of the State of Iowa, 1906 (31st General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Cummins delivered at the State Capitol.

Condition of the State of Iowa, 1923 (40th General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Kendall delivered at the State Capitol.

Condition of the State of Iowa, 1927 (42nd General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Hammill delivered at the State Capitol.

Condition of the State of Iowa, 1929 (43rd General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Hammill delivered at the State Capitol.

Condition of the State of Iowa, 1931 (44th General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Hammill delivered at the State Capitol.

Condition of the State of Iowa, 1933 (45th General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Turner delivered at the State Capitol.

Condition of the State of Iowa, 1935 (46th General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Herring delivered at the State Capitol.

Condition of the State of Iowa, 1937 (47th General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Herring delivered at the State Capitol.

Condition of the State of Iowa, 1939 (48th General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Kraschel delivered at the State Capitol.

Condition of the State of Iowa, 1941 (49th General Assembly)

A transcript of the Condition of the State of Iowa speech by Governor Wilson delivered at the State Capitol.