Aspectos pedológicos relacionados à irrigação em tabuleiros costeiros (Platô de Neópolis-SE)

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Terra e vida: a geografia dos camponeses no norte do Paraná

Pós-graduação em Geografia - FCT

Alfabetização matemática no contexto ribeirinho: um olhar sobre as classes multisseriadas da realidade amazônica

Esta dissertação, intitulada “Alfabetização matemática no contexto ribeirinho: um olhar sobre as classes multisseriadas da realidade amazônica”, teve como objetivo investigar abordagens teórico-metodológicas para o ensino e aprendizagem de matemática, no âmbito da alfabetização matemática, possíveis de serem estabelecidas em classes multisseriadas de escolas ribeirinhas da realidade amazônica. Nesta pesquisa, a visão de alfabetização matemática foi construída levando em consideração não só as primeiras habilidades de leitura e escrita inicial da linguagem matemática escolar pela criança (DANYLUK, 1997), mas como um caminho para a aprendizagem do homem e mulher no mundo das relações matemáticas, a partir de concepções teóricas de D‟Ambrosio (2002), Domite e Mesquita (2003), Sebastiani Ferreira (1997), I. Mendes (2009) e Freire (2002). A pesquisa foi realizada no município de São Domingos do Capim, Estado do Pará, Brasil, entre outubro de 2010 e agosto de 2011. Na investigação sobre a alfabetização matemática em classes multisseriadas de escolas ribeirinhas, foram realizadas interações com representantes da Secretaria Municipal de Educação, visitas às escolas ribeirinhas multisseriadas, entrevistas com professores deste município. Como instrumentos de construção de dados foram utilizados diário de campo, entrevistas e observações. A análise da pesquisa de campo foi organizada nos seguintes itens: a) As classes multisseriadas e a alfabetização matemática no contexto ribeirinho; b) Recursos didáticos e alfabetização matemática; e, c) Cultura e educação matemática na Amazônia: entre saberes científicos e saberes da tradição. É possível afirmar que a alfabetização matemática expressa no contexto ribeirinho ainda restringe-se as experiências matemáticas referentes estritamente ao currículo escolar sem levar em consideração a riqueza de possibilidades de conhecimentos das experiências dos estudantes em seu contexto diário como localização espacial no deslocamento pelos rios, nos elementos matemáticos envolvidos na comercialização de frutos e pescado, por exemplo.

O Programa de Microbacias no contexto do desenvolvimento rural da região de Jales-SP

Pós-graduação em Geografia - FCT

Pitaya: propagação e crescimento de plantas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Doses de fósforo na produção e qualidade de frutos de melão amarelo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Enxertia e testes de resistência à Ceratocystis fimbriata em variedades de Figueira (Ficus carica L)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Assessing melatonin and its oxidative metabolites amounts in biological fluid and culture medium by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento de Zaprionus indianus Gupta, 1970 (Diptera: Drosophilidae) em diferentes dietas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fenologia reprodutiva da pitaya vermelha em Jaboticabal, SP

The dragon fruit (Hylocereus undatus) is recent crop cultivation in Brazil and there is still lack of studies to support the farmers. So, this research aimed to characterize the reproductive phenology of the crop in the region of Jaboticabal, Sao Paulo State, Brazil. It was evaluated red dragon fruit clones over two environmental conditions - under plastic screen black and white, with 50% of shady level, from March 2009 to December 2010. It was observed that the issuance of floral buds and the flowering on dragon fruit culture occurs with a combination of high temperatures and rainfall, with constant emission of floral buds from November to March while the flowering on dragon fruit culture occurs until mid-April. The color of plastic screen had influenced on amount of flowers. The time elapsed since the issuance of flower buds to anthesis is from 18 to 23 days, while the harvest occurs from 34 to 43 days after flower opening. At Jaboticabal, the time of appearance of flower bud to fruit harvest is from 52 to 66 days.

Embalagem para produtos da fruticultura: proposta para uma reflexão sistêmica

This research is based on the reflection about the current culture of fruit packages, the standard that states the format and its materials, in the context of a contemporaneous design. It covers space representation issues, material and form representation in addition to the conditioner of the format of the pack as standard load and not the product itself. To analyze the factors related to packaging, we studied the systematic thinking, holistic or ecological and data were arranged in the form of systemic mind maps. The research aims to contribute with information to help in diagnosing the problem in its complexity and interconnected web of factors in order to facilitate better planning of packaging for fruit culture in a future sustainable society.

Custo energético da produção do maracujá amarelo na região de Marília-SP

The study evaluated the energy balance of the yellow passion fruit (Passiflora edulis Sims) production system in the region of Marilia-SP. There were analyzed the "inputs" of energy originated from biological, fossil, and industrial sources, and the "output" of energy in the form of fruits produced per unit area, as well as five energy indicators. The phytotechnical indices considered were: planting in February (seedling in tubes), production cycle: 18 months, and yield: 20 t/ha. The input energy totaled 173,707.86 MJ/ha. The most representative items corresponded to industrial indirect energy (38%) and to biological (34%). Chemical fertilizers and pesticides accounted for 65% and 31% of indirect energy industry, respectively. The activity generated 391,800.00 MJ per cycle/ha, and the culture productivity, 0.12 MJ/kg. The efficiency of the culture was 2.26. The net cultural energy totaled 218,092.15 MJ/ha. The energy balance was positive (126,050.23 MJ/ha). Energy efficiency was favorable, with a value of 3.64. The direct power consumption of fossil origin is significant in the system evaluated, but it still allows the production of the yellow passion fruit in an environmentally sustainable way.

Bacterial leakage along the implant-abutment interface: culture and DNA Checkerboard hybridization analyses

Objective Bacterial species have been found harboring the internal surface of dental implants as consequence of their failed connections. The aim of the present study was to compare the detection frequency of bacterial leakage from human saliva through the implantabutment interface, under non-loading conditions, using either DNA Checkerboard or culture method. Materials and methods Thirty dental implants with hexagonal platforms were connected to pre-machined abutments according to the manufacturers specifications. The assemblies were individually incubated in human saliva under anaerobic conditions for 7 similar to days at 37 degrees C. Afterward, contents from the inner parts of the implants were collected and evaluated with either DNA Checkerboard (s similar to=similar to 15) or culture (n similar to=similar to 15). Subsequently, identification and quantitation of bacterial species from saliva and implants were carried out for the group evaluated with the DNA Checkerboard method. Results Both DNA Checkerboard and culture showed positive signals of bacterial leakage in 6 of the 15 evaluated samples. Capnocytophaga gingivalis and Streptococcus mutans were the most frequently detected species harboring the internal surface of the implants followed by Veillonella parvula. Conclusion Occurrence of bacterial leakage along the implantabutment interface is comparably detected with both DNA Checkerboard hybridization and conventional culture methods.

Phenotypic and functional analysis of human fetal liver hematopoietic stem cells in culture

Steady-state hematopoiesis and hematopoietic transplantation rely on the unique potential of stem cells to undergo both self-renewal and multilineage differentiation. Fetal liver (FL) represents a promising alternative source of hematopoietic stem cells (HSCs), but limited by the total cell number obtained in a typical harvest. We reported that human FL nonobese diabetic/severe combined immunodeficient (NOD/SCID) repopulating cells (SRCs) could be expanded under simple stroma-free culture conditions. Here, we sought to further characterize FL HSC/SRCs phenotypically and functionally before and following culture. Unexpanded or cultured FL cell suspensions were separated into various subpopulations. These were tested for long-term culture potential and for in vivo repopulating function following transplantation into NOD/SCID mice. We found that upon culture of human FL cells, a tight association between classical stem cell phenotypes, such as CD34(+) /CD38(-) and/or side population, and NOD/SCID repopulating function was lost, as observed with other sources. Although SRC activity before and following culture consistently correlated with the presence of a CD34(+) cell population, we provide evidence that, contrary to umbilical cord blood and adult sources, stem cells present in both CD34(+) and CD34(-) FL populations can sustain long-term hematopoietic cultures. Furthermore, upon additional culture, CD34-depleted cell suspensions, devoid of SRCs, regenerated a population of CD34(+) cells possessing SRC function. Our studies suggest that compared to neonatal and adult sources, the phenotypical characteristics of putative human FL HSCs may be less strictly defined, and reinforce the accumulated evidence that human FL represents a unique, valuable alternative and highly proliferative source of HSCs for clinical applications.

Nonviral Gene Delivery of Growth and Differentiation Factor 5 to Human Mesenchymal Stem Cells Injected into a 3D Bovine Intervertebral Disc Organ Culture System

Intervertebral disc (IVD) cell therapy with unconditioned 2D expanded mesenchymal stem cells (MSC) is a promising concept yet challenging to realize. Differentiation of MSCs by nonviral gene delivery of growth and differentiation factor 5 (GDF5) by electroporation mediated gene transfer could be an excellent source for cell transplantation. Human MSCs were harvested from bone marrow aspirate and GDF5 gene transfer was achieved by in vitro electroporation. Transfected cells were cultured as monolayers and as 3D cultures in 1.2% alginate bead culture. MSC expressed GDF5 efficiently for up to 21 days. The combination of GDF5 gene transfer and 3D culture in alginate showed an upregulation of aggrecan and SOX9, two markers for chondrogenesis, and KRT19 as a marker for discogenesis compared to untransfected cells. The cells encapsulated in alginate produced more proteoglycans expressed in GAG/DNA ratio. Furthermore, GDF5 transfected MCS injected into an IVD papain degeneration organ culture model showed a partial recovery of the GAG/DNA ratio after 7 days. In this study we demonstrate the potential of GDF5 transfected MSC as a promising approach for clinical translation for disc regeneration.