Plan de Marketing de la Empresa Almadía S.L.

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Plan de marketing Industrias Lotu S.A.

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Plan de marketing Berlys

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Continuación de la asesoría en rescate, desarrollo de nuevos productos con carácter utilitario. Plan para el mejoramiento del sistema de quema. Desarrollo y edición de la etiqueta empaque y sistema de embalaje.

60 hojas.

Plan general de trabajo : departamento del Choco comunidades: Nuqui, Arusi, Jurubida, El Valle, Acandí.

[50] hojas : ilustraciones.

Diseño y desarrollo de producto : diseño plan de producción.

6 hojas : ilustraciones, fotografías.

Informe Final de actividades relacionadas en el plan de trabajo del contrato interinstitucional suscrito entre Artesanías de Colombia S. A. y la Alcaldía de Palmira, Valle del Cauca.

40 hojas : ilustraciones fotografías.

Proyecto plan de vida Puerto Asís (Artesanos independientes) Putumayo.

18 hojas : Ilustraciones.

[Plan de negocio para la asociación de joyeros Asojomar de Marmato].

30 hojas : ilustraciones

Phosphorus export from a restored wetland ecosystem in response to natural and experimental hydrologic fluctuations

Wetland restoration is a commonly used approach to reduce nutrient loading to freshwater and coastal ecosystems, with many wetland restoration efforts occurring in former agricultural fields. Restored wetlands are expected to be effective at retaining or removing both nitrogen and phosphorus (P), yet restoring wetland hydrology to former agricultural fields can lead to the release of legacy fertilizer P. Here, we examined P cycling and export following rewetting of the Timberlake Restoration Project, a 440 ha restored riverine wetland complex in the coastal plain of North Carolina. We also compared P cycling within the restored wetland to two minimally disturbed nearby wetlands and an adjacent active agricultural field. In the restored wetland we observed increased soluble reactive phosphorus (SRP) concentrations following initial flooding, consistent with our expectations that P bound to iron would be released under reducing conditions. SRP concentrations in spring were 2.5 times higher leaving the restored wetland than a forested wetland and an agricultural field. During two large-scale drawdown and rewetting experiments we decreased the water depth by 1 m in ∼10 ha of inundated wetland for 2 weeks, followed by reflooding. Rewetting following experimental drainage had no effect on SRP concentrations in winter, but SRP concentrations did increase when the experiment was repeated during summer. Our best estimates suggest that this restored wetland could release legacy fertilizer P for up to a decade following hydrologic restoration. The time lag between restoration and biogeochemical recovery should be incorporated into management strategies of restored wetlands. Copyright 2010 by the American Geophysical Union.

Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1.

Activation of the Cyclin B/Cdc2 kinase complex triggers entry into mitosis in all eukaryotic cells. Cyclin B1 localization changes dramatically during the cell cycle, precipitously transiting from the cytoplasm to the nucleus at the beginning of mitosis. Presumably, this relocalization promotes the phosphorylation of nuclear targets critical for chromatin condensation and nuclear envelope breakdown. We show here that the previously characterized cytoplasmic retention sequence of Cyclin B1, responsible for its interphase cytoplasmic localization, is actually an autonomous nuclear export sequence, capable of directing nuclear export of a heterologous protein, and able to bind specifically to the recently identified export mediator, CRM1. We propose that the observed cytoplasmic localization of Cyclin B1 during interphase reflects the equilibrium between ongoing nuclear import and rapid CRM1-mediated export. In support of this hypothesis, we found that treatment of cells with leptomycin B, which disrupted Cyclin B1-CRM1 interactions, led to a marked nuclear accumulation of Cyclin B1. In mitosis, Cyclin B1 undergoes phosphorylation at several sites, a subset of which have been proposed to play a role in Cyclin B1 accumulation in the nucleus. Both CRM1 binding and the ability to direct nuclear export were affected by mutation of these phosphorylation sites; thus, we propose that Cyclin B1 phosphorylation at the G2/M transition prevents its interaction with CRM1, thereby reducing nuclear export and facilitating nuclear accumulation.

Sea Urchin Body Plan Development and Evolution: An Integrative Transcriptomic Approach

My dissertation work integrates comparative transcriptomics and functional analyses to investigate gene expression changes underlying two significant aspects of sea urchin evolution and development: the dramatic developmental changes associated with an ecologically significant shift in life history strategy and the development of the unusual radial body plan of adult sea urchins.

In Chapter 2, I investigate evolutionary changes in gene expression underlying the switch from feeding (planktotrophic) to nonfeeding (lecithotrophic) development in sea urchins. In order to identify these changes, I used Illumina RNA-seq to measure expression dynamics across 7 developmental stages in three sea urchin species: the lecithotroph Heliocidaris erythrogramma, the closely related planktotroph Heliocidaris tuberculata, and an outgroup planktotroph Lytechinus variegatus. My analyses draw on a well-characterized developmental gene regulatory network (GRN) in sea urchins to understand how the ancestral planktotrophic developmental program was altered during the evolution of lecithotrophic development. My results suggest that changes in gene expression profiles occurred more frequently across the transcriptome during the evolution of lecithotrophy than during the persistence of planktotrophy. These changes were even more pronounced within the GRN than across the transcriptome as a whole, and occurred in each network territory (skeletogenic, endomesoderm and ectoderm). I found evidence for both conservation and divergence of regulatory interactions in the network, as well as significant changes in the expression of genes with known roles in larval skeletogenesis, which is dramatically altered in lecithotrophs. I further explored network dynamics between species using coexpression analyses, which allowed me to identify novel players likely involved in sea urchin neurogenesis and endoderm patterning.

In Chapter 3, I investigate developmental changes in gene expression underlying radial body plan development and metamorphosis in H. erythrogramma. Using Illumina RNA-seq, I measured gene expression profiles across larval, metamorphic, and post-metamorphic life cycle phases. My results present a high-resolution view of gene expression dynamics during the complex transition from pre- to post-metamorphic development and suggest that distinct sets of regulatory and effector proteins are used during different life history phases.

Collectively, my investigations provide an important foundation for future, empirical studies to investigate the functional role of gene expression change in the evolution of developmental differences between species and also for the generation of the unusual radial body plan of sea urchins.

Plan de marketing Muebles Maldonado

El presente plan de marketing considera el portafolio de MUEBLES MALDONADO S.A.C. empresa que cuenta con el respaldo de más de 22 años de experiencia y trabajo, desarrolla sus actividades en el sector maderero tanto de fabricación como comercialización de muebles teniendo como línea de productos muebles de sala, comedor, dormitorio y accesorios decorativos; perfilado con diseñados novedosos y exclusivos, muebles lanzados al mercado con las mejores maderas (tornillo, pino, caoba) y de la mejor calidad y precios acorde a productos (diseños o modelos). Cuenta con un equipo de personal eficiente y apto tanto en la fabricación de los productos al cual se debe gran parte de sus éxito como en el área de ventas para satisfacer las necesidades y deseos de sus clientes actuales y potenciales en un futuro; teniendo como competencia a las empresas en productos sustitutos y a la competencia directa. Se analiza la situación del mercado para conocer el mercado objetivo y se describe las estrategias del marketing estratégico y operativo para diagnosticar la situación actual del marketing de la empresa MUEBLES MALDONADO. Además, se tienen en cuenta a los principales competidores, las estrategias, los canales de distribución y el análisis de oportunidades y alternativas de los productos

D9.1 - RAGE Dissemination Plan

This Dissemination Plan aims to guide and support the efforts of each and all RAGE partners in convincing Applied Games stakeholders to use the outputs of the Project. This is a live document as it will be periodically updated, enhanced and completed along the entire RAGE project lifecycle.

D10.2 - Research Data Management Plan, version 1.0

This document is the first out of three iterations of the DMP that will be formally delivered during the project. Version 2 is due in month 24 and version 3 towards the end of the project. The DMP thus is not a fixed document; it evolves and gains more precision and substance during the lifespan of the project. In this first version we describe the planned research data sets related to the RAGE evaluation and validation activities, and the fifteen principles that will guide data management in RAGE. The former are described in the format of the EU data management template, and the latter in terms of their guiding principle, how we propose to implement them, and when they will be implemented. This document is thus first of all relevant to WP5 and WP8 members.