Zambujeira e Serra do Calvo: uma equipa júnior de futsal em evolução

RESUMO: O Futsal é uma modalidade em plena ascensão e com crescente popularidade em Portugal e no Mundo, com a particularidade de ser a modalidade com mais praticantes femininos em Portugal. Neste relatório pretendo apresentar a forma como interpreto o jogo e o treino de Futsal, aplicando os conhecimentos alcançados ao longo de dois anos de estudo da modalidade, no meu percurso académico e nos seis anos como treinador principal. Ao longo do relatório irei evidenciar os aspectos fundamentais da minha visão sobre o processo de treino através da elaboração de um Modelo de Jogo coerente e adequado à realidade do Grupo Desportivo Cultural e Recreativo Zambujeira e Serra do Calvo, no escalão de Júnior “A”. Cabe ao treinador a criação de um Modelo de Jogo a atingir, em que este irá direccionar todo o processo de treino através de intervenções ajustadas à jogadora de acordo com situação em que está inserida. Ao longo do processo de treino, o treinador tem que saber observar e analisar os contextos em que o clube, equipa e competição se inserem, de modo a que consiga elaborar e dotar o planeamento de uma flexibilidade desejável. ABSTRACT: The Futsal is a sport booming in popularity in Portugal and the World, with the distinction of being the sport with more female athlete’s in Portugal. In this report, I intend to present the way I play the game of Futsal, by training and applying the knowledge, achieved over two years of studying the sport during my academic background and over six years as head coach. Throughout the report will highlight the fundamental aspects of my vision of the training process by developing a coherent model of game and suitable for the reality of the Cultural and Recreational Sports Group Zambujeira E Serra do Calvo in Junior "A" team. It is the coach responsibility to create a game model to be achieved this is the model that will direct the entire training process through adjusted interventions to the player according to the situation in which it operates. Throughout the training process, the coach has to know how to observe and analyze the contexts in which the club, team and competition is been held, so he can prepare and provide the planning of a desirable flexibility if necessary the possible changes.

Construction of a Festuca pratensis BAC library for map-based cloning in Festulolium substitution lines

Introgression in Festulolium is a potentially powerful tool to isolate genes for a large number of traits which differ between Festuca pratensis Huds. and Lolium perenne L. Not only are hybrids between the two species fertile, but the two genomes can be distinguished by genomic in situ hybridisation and a high frequency of recombination occurs between homoeologous chromosomes and chromosome segments. By a programme of introgression and a series of backcrosses, L. perenne lines have been produced which contain small F. pratensis substitutions. This material is a rich source of polymorphic markers targeted towards any trait carried on the F. pratensis substitution not observed in the L. perenne background. We describe here the construction of an F. pratensis BAC library, which establishes the basis of a map-based cloning strategy in L. perenne. The library contains 49,152 clones, with an average insert size of 112 kbp, providing coverage of 2.5 haploid genome equivalents. We have screened the library for eight amplified fragment length polymorphism (AFLP) derived markers known to be linked to an F. pratensis gene introgressed into L. perenne and conferring a staygreen phenotype as a consequence of a mutation in primary chlorophyll catabolism. While for four of the markers it was possible to identify bacterial artificial chromosome (BAC) clones, the other four AFLPs were too repetitive to enable reliable identification of locus-specific BACs. Moreover, when the four BACs were partially sequenced, no obvious coding regions could be identified. This contrasted to BACs identified using cDNA sequences, when multiple genes were identified on the same BAC.

A maize bacterial artificial chromosome (BAC) library from the European flint inbred line F2

We report here the construction and characterisation of a BAC library from the maize flint inbred line F2, widely used in European maize breeding programs. The library contains 86,858 clones with an average insert size of approximately 90 kb, giving approximately 3.2-times genome coverage. High-efficiency BAC cloning was achieved through the use of a single size selection for the high-molecular-weight genomic DNA, and co-transformation of the ligation with yeast tRNA to optimise transformation efficiency. Characterisation of the library showed that less than 0.5% of the clones contained no inserts, while 5.52% of clones consisted of chloroplast DNA. The library was gridded onto 29 nylon filters in a double-spotted 8 × 8 array, and screened by hybridisation with a number of single-copy and gene-family probes. A 3-dimensional DNA pooling scheme was used to allow rapid PCR screening of the library based on primer pairs from simple sequence repeat (SSR) and expressed sequence tag (EST) markers. Positive clones were obtained in all hybridisation and PCR screens carried out so far. Six BAC clones, which hybridised to a portion of the cloned Rp1-D rust resistance gene, were further characterised and found to form contigs covering most of this complex resistance locus.

Redescription of Culex (Culex) dolosus (Lynch Arribalzaga) (Diptera : Culicidae), based on specimens from Pico do Itapeva, Serra da Mantiqueira, Sao Paulo, Brazil

Culex (Culex) dolosus (Lynch Arribalzaga, 1891) is re-described and compared with Cx. eduardoi Casal & Garcia ( 1968) based on morphological characteristics. Both species are morphologically similar, and they have been largely misidentified throughout Brazil. Adult male and female, fourth instar larvae, and pupae of Cx. dolosus were examined, based on specimens from Pico do Itapeva, Pindamonhangaba Municipality, Serra da Mantiqueira, Sao Paulo State, southern Brazil. Male genitalia, larvae and pupae are illustrated. Geographical distribution is summarized from published records, and information on bionomics is based on the literature and field data.