Caso empresarial: comparación entre las empresas Ecopetrol en Colombia y Petrobras en Brasil, desde los programas de RSE que coadyuvan al mejoramiento competitivo en el periodo 2008-2012

Bogotá (Colombia) : Universidad de la Salle. Facultad de Ciencias Económicas y Sociales. Programa de Finanzas y Comercio Internacional

Alteraciones de la calidad y cantidad de la produccion de leche en presencia de map a traves de ELISA PPA

La paratuberculosis (PTB) o enfermedad de Johne es una enteritis granulomatosa infecto-contagiosa de curso crónico e incurable que afecta a los rumiantes, causada por Mycobacterium avium spp paratuberculosis (Map). Las pérdidas económicas por PTB se dan debido a la reducción de las producciones cárnica y láctea, disminución de la fertilidad, incremento en la incidencia de mastitis, menor expectativa de vida productiva de la hembra, eliminación prematura de animales, etc. El objetivo del trabajo es evaluar el impacto de la infección de Map identificada mediante la detección de anticuerpos en la leche, sobre la producción de leche (cantidad y calidad) a partir del estudio individual de las hembras en producción.

Detección serológica por Elisa indirecta de hemoparásitos y dirofilaria immitis en caninos en Bogotá, Colombia

Tesis (Médico Veterinario). -- Universidad de La Salle. Facultad de Ciencias Agropecuarias. Programa de Medicina Veterinaria, 2013

La gestión de procesos y el desempeño competitivo de las PYMES

En este artículo se añalizan los factores internos de las Pequeñas y Medianas empresas, PyMEs, del sector manufactura, con el enfoque hacia la gestión del proceso operativo, el cual es determinante en la competitividad de las empresas.

Poemas de Elisa Logan

- Convite- A desatiempo

Prevalencia de leucosis viral bovina en ganado Holstein lechero en el municipio de pacho Cundinamarca, vereda el Hatillo mediante Elisa indirecta

Bogotá (Colombia): Universidad de La Salle. Facultad de Ciencias Agropecuarias. Programa de Medicina Veterinaria

Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA

In the absence of effective vaccine(s), control of African swine fever caused by African swine fever virus (ASFV) must be based on early, efficient, cost-effective detection and strict control and elimination strategies. For this purpose, we developed an indirect ELISA capable of detecting ASFV antibodies in either serum or oral fluid specimens. The recombinant protein used in the ELISA was selected by comparing the early serum antibody response of ASFV-infected pigs (NHV-p68 isolate) to three major recombinant polypeptides (p30, p54, p72) using a multiplex fluorescent microbead-based immunoassay (FMIA). Non-hazardous (non-infectious) antibody-positive serum for use as plate positive controls and for the calculation of sample-to-positive (S:P) ratios was produced by inoculating pigs with a replicon particle (RP) vaccine expressing the ASFV p30 gene. The optimized ELISA detected anti-p30 antibodies in serum and/or oral fluid samples from pigs inoculated with ASFV under experimental conditions beginning 8 to 12 days post inoculation. Tests on serum (n = 200) and oral fluid (n = 200) field samples from an ASFV-free population demonstrated that the assay was highly diagnostically specific. The convenience and diagnostic utility of oral fluid sampling combined with the flexibility to test either serum or oral fluid on the same platform suggests that this assay will be highly useful under the conditions for which OIE recommends ASFV antibody surveillance, i.e., in ASFV-endemic areas and for the detection of infections with ASFV isolates of low virulence.

A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus

BACKGROUND Elephants are classified as critically endangered animals by the International Union for Conservation of Species (IUCN). Elephant endotheliotropic herpesvirus (EEHV) poses a large threat to breeding programs of captive Asian elephants by causing fatal haemorrhagic disease. EEHV infection is detected by PCR in samples from both clinically ill and asymptomatic elephants with an active infection, whereas latent carriers can be distinguished exclusively via serological assays. To date, identification of latent carriers has been challenging, since there are no serological assays capable of detecting seropositive elephants. RESULTS Here we describe a novel ELISA that specifically detects EEHV antibodies circulating in Asian elephant plasma/serum. Approximately 80 % of PCR positive elephants display EEHV-specific antibodies. Monitoring three Asian elephant herds from European zoos revealed that the serostatus of elephants within a herd varied from non-detectable to high titers. The antibody titers showed typical herpes-like rise-and-fall patterns in time which occur in all seropositive animals in the herd more or less simultaneously. CONCLUSIONS This study shows that the developed ELISA is suitable to detect antibodies specific to EEHV. It allows study of EEHV seroprevalence in Asian elephants. Results confirm that EEHV prevalence among Asian elephants (whether captive-born or wild-caught) is high.

TRIBUNAL ARBITRAL DE MARÍA YANNETH CHAUTA PINILLA Y MARTHA VICTORIA CHAUTA GONZÁLEZ VS. LEONARDO LEÓN CHAUTA IBARRA, MAYRA ALEJANDRA CHAUTA IBARRA Y MARÍA ELISA IBARRA RAMÍREZ

3992

El Diálogo Competitivo como instrumento para la maduración de proyectos de infraestructura vial

En Colombia, mediante la Ley 1508 de 2102, se estableció el régimen jurídico de las Asociaciones Público Privadas (APP) y con la aprobación de la Ley 1682 de 2013, se regularon los proyectos de infraestructura de transporte. Con la presente tesis se pretende demostrar la figura del Diálogo Competitivo como procedimiento incompleto e insuficiente, apropiado así por nuestro régimen jurídico en la Ley de APP, visto desde la perspectiva de la Unión Europea, especialmente de los modelos español, inglés y francés, porque carece de la mayoría de sus elementos característicos. Posteriormente se realiza una crítica al Diálogo Competitivo colombiano desde la perspectiva de la Unión Europea, ya que se evidencia en la figura interna su precariedad frente a un procedimiento debidamente definido y organizado como el de la Unión Europea. Entre las instituciones jurídicas adoptadas por la Ley de APP, se encuentra el Diálogo Competitivo, que es un procedimiento, verificado en la etapa de estructuración de los contratos APP de iniciativa pública, cuyos antecedentes registran el mayor reconocimiento internacional en la Unión Europea (UE). Ésta introdujo esa figura a su régimen jurídico, a través de la Directiva 2004/18/CE, a su vez derogada por la Directiva 2014/24/UCE. La tesis culmina con una serie de conclusiones y recomendaciones que se estiman útiles para repensar y reestructurar el Diálogo Competitivo en los proyectos de infraestructura vial en Colombia, tarea que le corresponde al Congreso Nacional, pues así lo ha dispuesta la jurisprudencia del Consejo de Estado.

Sindicatos Políticos y Economía (1972-1986) San José: Editorial Alma Mater, 1987. (Elisa Donato y Manuel Rojas)

Introducción Sindicatos, política y economía (1972-1986) es un interesante libro sobre la situación del movimiento sindical costarricense y sus perspectivas futuras. Se divide en siete capítulos: el movimiento sindical después de 1978,  el nivel de vida, características estructurales del movimiento sindical en el periodo sindical 1972-1984,  la actividad del movimiento sindical en el periodo (1972-1984),  el estado y el sindicalismo, Sindicatos, empresarios y sacerdotes. El libro muestra un excelente uso de fuentes estadísticas, una buena radiografía del movimiento sindical durante los años 1972-1986, pero como los mismos autores lo señalan “los números solo son parte de la realidad”. A nuestro juicio, los autores no contextualizan, lo suficiente, los datos que arrojan las estadísticas del Ministerio de Trabajo y Seguridad Social.

L'ibridazione degli OTT. Adattamento competitivo, strategie manageriali e retoriche

Il presente studio analizza l’attuale tendenza del sistema televisivo statunitense all’ibridazione con i modelli affermati già nell’era dominata dai network prima, e dalla CATV poi. Dopo una mappatura storica che permette di comprendere e apprezzare i continui cambiamenti dell’industria televisiva, lo scritto si concentra sull’analisi delle varie strategie manageriali (e sulle retoriche) che stanno alla base delle prime fasi dei servizi non lineari. Si esaminano inoltre le modalità che governano ora i tentativi di recupero di esperienze che sembravano ormai superate per lasciare spazio proprio ai modelli OTT. Infatti, data la non sostenibilità sul lungo periodo delle forme economiche, distributive, produttive, etc., adottate delle piattaforme streaming come Netflix, Amazon Prime Video e Disney+, queste cercano ora di ricostruire una dimensione lineare, alla cui matrice troviamo la weekly release, la pubblicità, la sincronizzazione sociale, la diretta e altro ancora – si pensi ai casi Pluto TV e Peacock TV, esempi di servizi HVOD (Hybrid Video on Demand) che stanno tracciando dei nuovi percorsi pur senza dimenticare ciò che ha funzionato in passato.

Testosterone Therapy Differently Regulates The Anti- And Pro-inflammatory Cytokines In The Plasma And Prostate Of Rats Submitted To Chronic Ethanol Consumption (uchb).

Ethanol consumption damages the prostate, and testosterone is known by anti-inflammatory role. The cytokines were investigated in the plasma and ventral prostate of UChB rats submitted or not to testosterone therapy by ELISA and Western blot, respectively. Additionally, inflammatory foci and mast cells were identified in the ventral prostate slides stained by hematoxylin and eosin and toluidine blue, respectively. Inflammatory foci were found in the ethanol-treated animals and absent after testosterone therapy. Plasma levels of IL-6 and IL-10 were not changed while TNFα and TFG-β1 were increased in the animals submitted testosterone therapy. Regarding to ventral prostate, IL-6 did not alter, while IL-10, TNFα, and TFG-β1 were increased after testosterone therapy. Ethanol increases NFR2 in addition to high number of intact and degranulated mast cell which were reduced after testosterone therapy. So, ethanol and testosterone differentially modulates the cytokines in the plasma and prostate.

Profile Of The Use Of Disease Modifying Drugs In The Brazilian Registry Of Spondyloarthritides.

Few studies have evaluated the profile of use of disease modifying drugs (DMD) in Brazilian patients with spondyloarthritis (SpA). A common research protocol was applied prospectively in 1505 patients classified as SpA by criteria of the European Spondyloarthropathies Study Group (ESSG), followed at 29 referral centers in Rheumatology in Brazil. Demographic and clinical variables were obtained and evaluated, by analyzing their correlation with the use of DMDs methotrexate (MTX) and sulfasalazine (SSZ). At least one DMD was used by 73.6% of patients: MTX by 29.2% and SSZ by 21.7%, while 22.7% used both drugs. The use of MTX was significantly associated with peripheral involvement, and SSZ was associated with axial involvement, and the two drugs were more administered, separately or in combination, in the mixed involvement (p < 0.001). The use of a DMD was significantly associated with Caucasian ethnicity (MTX , p = 0.014), inflammatory back pain (SSZ, p = 0.002) , buttock pain (SSZ, p = 0.030), neck pain (MTX, p = 0.042), arthritis of the lower limbs (MTX, p < 0.001), arthritis of the upper limbs (MTX, p < 0.001), enthesitis (p = 0.007), dactylitis (MTX, p < 0.001), inflammatory bowel disease (SSZ, p < 0.001) and nail involvement (MTX, p < 0.001). The use of at least one DMD was reported by more than 70% of patients in a large cohort of Brazilian patients with SpA, with MTX use more associated with peripheral involvement and the use of SSZ more associated with axial involvement.

A Putative Otu Domain-containing Protein 1 Deubiquitinating Enzyme Is Differentially Expressed In Thyroid Cancer And Identifies Less-aggressive Tumours.

This study aimed to identify novel biomarkers for thyroid carcinoma diagnosis and prognosis. We have constructed a human single-chain variable fragment (scFv) antibody library that was selected against tumour thyroid cells using the BRASIL method (biopanning and rapid analysis of selective interactive ligands) and phage display technology. One highly reactive clone, scFv-C1, with specific binding to papillary thyroid tumour proteins was confirmed by ELISA, which was further tested against a tissue microarray that comprised of 229 thyroid tissues, including: 110 carcinomas (38 papillary thyroid carcinomas (PTCs), 42 follicular carcinomas, 30 follicular variants of PTC), 18 normal thyroid tissues, 49 nodular goitres (NG) and 52 follicular adenomas. The scFv-C1 was able to distinguish carcinomas from benign lesions (P=0.0001) and reacted preferentially against T1 and T2 tumour stages (P=0.0108). We have further identified an OTU domain-containing protein 1, DUBA-7 deubiquitinating enzyme as the scFv-binding antigen using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. The strategy of screening and identifying a cell-surface-binding antibody against thyroid tissues was highly effective and resulted in a useful biomarker that recognises malignancy among thyroid nodules and may help identify lower-risk cases that can benefit from less-aggressive management.