1000 resultados para Contaminação microbiológica
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Pós-graduação em Biologia Geral e Aplicada - IBB
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Cirurgia Veterinária - FCAV
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Geociências e Meio Ambiente - IGCE
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Pós-graduação em Medicina Veterinária - FCAV
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Pós-graduação em Química - IQ
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Background: The use of all by-products of bovine slaughter is of high economic importance for the industries of products of animal origin. Among these products, fat has an important role, once fat rendering may generate several different products, such as protein material that may be used in the manufacture of meat products. However, in spite of the importance that the use of all by-products has for the economic balance of the industry, there are no reports on their use in Brazil, or studies that supply data on microbiological and physical-chemical local standards for this protein. Thus, the objective of this study was to evaluate microbiological and physical-chemical characteristics of protein material obtained from fat rendering, as well as to provide support for companies to use fat rendering to generate protein material, adding value to industrialized meat products.Materials, Methods & Results: The experimental production of edible protein obtained of fat rendering was conducted in slaughterhouse with supervision of the Brazilian Ministry of Agriculture, Livestock and Food Supply. Protein material was obtained in a continuous, humid heat system at high temperatures. Fat scraps containing protein were ground and cooked at high temperature (85 degrees C), and placed in a three phase decanter centrifuge. After centrifugation, protein material was ground again and packed. Samples were collected from 15 batches of protein material, and the following microbiological analyses were carried out: counts of aerobic mesophilic and psychrotrophic microorganisms, coliforms at 35 degrees C, Escherichia coli, sulfite-reducing Clostridium, and Staphylococcus aureus, besides presence or absence of Salmonella and Listeria monocytogens. The following physical-chemical analyses were also carried out: protein, total lipid, moisture, ash, carbohydrate, and energy content. Mean counts of mesophiles, psychrotrophs, and coliforms at 35 degrees C were 4.17; 3.69 and 1.87 (log CFU/g), respectively. Levels of protein, total lipids, moisture, ashes and carbohydrates were 27.50; 7.83; 63.88%; 0.24%; and 0.55%, respectively, and energy content was 182.63 kcal/100g.Discussion: Results of microbiological analyses demonstrated that, although low, the final product showed to be contaminated. Contamination that occurred during the second grinding procedure may be an explanation for these bacterial counts. Also, the temperature used for fat fusion was not enough to eliminate thermoduric microorganisms. However, even with the presence of indicator microorganisms in the samples, none was contaminated by E. coli, sulfite-reducing Clostridium, S. aureus, Salmonella or L. monocytogenes. Physical-chemical analyses showed that the product had adequate nutritional quality. Based on these results, it was possible to conclude that protein material obtained in fat rendering showed characteristics that enable the use of this product as raw material for processed meat products. Besides, the present study was the first one to present scientific results in relation to edible by-products obtained in fat rendering, supplying important information for slaughterhouses and meat-processing plants. The study also produced relevant data on the innocuousness of the product, which may be used to guide decision-making of health inspectors.
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Pós-graduação em Microbiologia Agropecuária - FCAV
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Pós-graduação em Odontologia - FOAR
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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A pesquisa teve como objetivos gerais à aplicação dos métodos geoelétricos na hidrogeologia, visando: (1) estudos para captação de águas subterrâneas para abastecimento em geral; e, (2) estudos das contaminações de solos, rochas e águas subterrâneas, envolvendo as fases de investigação preventiva, confirmatória, remediação e monitoramento. Como objetivos específicos, através da aplicação dos métodos da eletrorresistividade e polarização induzida, utilizando-se das técnicas de campo da sondagem elétrica vertical - arranjo Schlumberger e caminhamento elétrico - arranjo dipolo-dipolo, e dos parâmetros de Dar Zarrouk, tem-se uma discussão e proposição metodológica e prática, em função da geologia local, sobre os produtos obtidos por essa metodologia, tais como: caracterização geológica na identificação de aqüíferos promissores e investigação de áreas contaminadas e/ou sujeitas à contaminação. Portanto, esse trabalho procurou apresentar uma discussão sobre os principais métodos e técnicas de campo dos métodos geoelétricos aplicados em estudos hidrogeológicos, com destaque na captação para abastecimento e diagnóstico de solos, rochas e águas subterrâneas frente a poluentes. A proposição metodológica apresentada, procurou aplicar uma relação entre a resolução de métodos e técnicas com custos e prazos adequados a um trabalho envolvendo a Geofísica Aplicada em estudos hidrogeológicos. O emprego da eletrorresistividade, com as técnicas da SEV e CE, por si só, revela-se decisivo para atingir os mais variados fins envolvendo esses tipos de estudos. A resistividade elétrica dos materiais geológicos, é definida, principalmente, em função de seus constituintes mineralógicos e o tipo de fluido presente, sendo, portanto, ideal para os estudos hidrogeológicos de maneira geral. Cabe ressaltar ainda... (Resumo completo, clicar acesso eletrônico abaixo)
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The contamination of underground waters is a subject of great importance, since a lot of people use this kind of source for public supply. This paperwork manages with how gas stations can contaminate these waters and what are some remediation techniques, used nowadays to solve this problem. The contamination caused by the gas stations can have its origin in the underground storage tanks when they suffer corrosion and show leaking problems. It’s a topic of great relevance, because the hydrocarbons derivated from petroleum present in the fuels can stay for a long time in the environment. Besides that, an analyses of the risk of contamination by gas stations in the city of Rio Claro was made based on three parameters: density of the gas stations, depth of the underground water and hydraulic conductivity. To achieve such goal, maps were elaborated considering those parameters individually and considering the three of them together. Analyzing those maps, it was concluded see that the spots that represent the biggest risks of contamination in Rio Claro are located southeast and center-west.
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Previous studies show that in areas contaminated by fuel spill (NAPL- non-aqueous phase liquids), from operational activities, transport and storage, it was possible to observe a significant decrease of ²²²Rn (radon) gas concentration in the soil, even a non-uniform distribution of this gas in top soil, even with a geological situation was practically homogeneous. These anomalies may be associated with the preference partitioning of radon in NAPLs. This work consists of applying ²²²Rn as an indicator for locating subsurface contamination by NAPLs in an area of the city of Rio Claro (SP) where, according to the “Survey of Contaminated and Rehabilitated Areas in the State of São Paulo (Environmental Sanitation and Technology Company - CETESB), there was, in the year 2007, groundwater contamination from leaks of liquid fuels. The challenges of this research are: Promulgate the use of a new tool with greater efficiency in obtaining results, in addition to generate less impact in half and have less expenditure; disseminate scientific culture promoting greater integration of C&T (culture & technology) between universities and businesses. The emanometric technique to estimate the location, number and interfacial area of NAPL in saturated and non-saturated zone, has the advantage of locating and determining plumes of free phase even when the amount of VOC's (Volatile Organic Compounds) that reaches the surface is low or non-existent. In addition, the measurement techniques ²²²Rn are quite developed. The results obtained show that, similar to the other studies, the 222Rn soil gas presents an anomalous behavior in the area bounded by NAPL plume, being possible to note a significant deficit in the concentration of the gas in spots where the saturation by NAPLs is still critical. Therefore it is concluded that this tool is really promising, but we must be careful to evaluate the initial conditions of the area, as well as the type of...
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A contaminação de rios com metais pesados vêm aumentando nas últimas décadas em resposta ao crescimento do setor industrial e seus dejetos, que frequentemente são despejados no ambiente sem que o tratamento adequado seja realizado. As implicações ambientais da contaminação dos ecossistemas naturais de água doce com níquel ainda são mal compreendidas, entretanto, a demanda global por este metal e as descargas antrópicas provenientes do setor industrial só aumentam. Embora o níquel não seja considerado altamente tóxico como outros metais, ele tem a capacidade de ser bioacumulativo e é, portanto, potencialmente perigoso para os peixes. Assim, é importante que seja desenvolvida uma compreensão mais profunda da fisiologia básica do níquel em peixes, tanto como um nutriente essencial quanto como um agente tóxico. Desse modo, o presente estudo teve como objetivo a análise do potencial genotóxico e mutagênico do níquel em peixes da espécie Oreochromis niloticus (Cichlidae), e a comparação das concentrações utilizadas deste metal nos bioensaios com a concentração limite, estabelecida pelo Conselho Nacional do Meio Ambiente (CONAMA). A resolução 357/05 do CONAMA, para controlar e taxar os limites máximos de contaminantes na água define que o limite máximo da concentração total de níquel permitida em corpos de água doce é de 0,025 mg/L Ni. Assim, os organismos-teste foram expostos a três diferentes concentrações de cloreto de níquel diluído, sendo que, a primeira concentração foi de 0,0125 mg/L, ou seja, metade da concentração máxima definida pelo CONAMA. A segunda concentração utilizada, 0,025 mg/L é o próprio limite de concentração estabelecido pela resolução. Por fim, a terceira concentração utilizada foi de 0,050 mg/L, que consiste no dobro da concentração máxima total de níquel permitida em corpos de água doce... (Resumo completo, clicar acesso eletrônico abaixo)