Satellyptus: analysis and database of microsatellites from ESTs of Eucalyptus

The main goal of our research was to search for SSRs in the Eucalyptus EST FORESTs database (using a software for mining SSR-motifs). With this objective, we created a database for cataloging Eucalyptus EST-derived SSRs, and developed a bioinformatics tool, named Satellyptus, for finding and analyzing microsatellites in the Eucalyptus EST database. The search for microsatellites in the FORESTs database containing 71,115 Eucalyptus EST sequences (52.09 Mb) revealed 20,530 SSRs in 15,621 ESTs. The SSR abundance detected on the Eucalyptus ESTs database (29% or one microsatellite every four sequences) is considered very high for plants. Amongst the categories of SSR motifs, the dimeric (37%) and trimeric ones (33%) predominated. The AG/CT motif was the most frequent (35.15%) followed by the trimeric CCG/CGG (12.81%). From a random sample of 1,217 sequences, 343 microsatellites in 265 SSR-containing sequences were identified. Approximately 48% of these ESTs containing microsatellites were homologous to proteins with known biological function. Most of the microsatellites detected in Eucalyptus ESTs were positioned at either the 5 or 3 end. Our next priority involves the design of flanking primers for codominant SSR loci, which could lead to the development of a set of microsatellite-based markers suitable for marker-assisted Eucalyptus breeding programs.

Avaliação do programa de atenção à saúde do trabalhador: uma abordagem da atenção primária

The accelerated industrialization, coming with the Industrial Revolution, caused profound changes in the working world. These changes led to the households risks from work environment. Trying to assist comprehensively the health of workers, Brazil has a program of Health Care Workers in the Primary Care, and the Family Health Strategy is the main entrance for this system. The study sought to determine if the actions of the health care worker have been developed in primary care through the Family Health Strategy. This is a quantitative study with a methodological evaluation, focusing on normative assessment. The sample was formed by professionals from Team Family Health Strategy, in the municipalities of Pau dos Ferros, Caicó and Natal in Rio Grande do Norte state. The sample consists of 202 professionals (Doctors, Nurses, Assistant / Technician Nursing and Community Health Workers) in 52 Health Family Units from the 3 municipalities cited. The instrument used consists of a checklist, from Manual of Primary Care 5 - Family Health - Occupational Health, Ministry of Health. The data were analyzed describing the variables by its frequency and doing a classification of cities from the scores obtained by each. It was observed that the Family Health professionals know the program of health care worker, however do not know the Manual of Primary Care 5, which is a guidance tool. As a result of non-appropriation of the FHT professionals with worker health, these activities are not performed, mainly surveillance in occupational health and health education labor

Uso das tecnologias na representação descritiva: o padrão de descrição bibliográfica semântica MarcOnt Initiative nos ambientes informacionais digitais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Uma proposta de framework como infra-estrutura de implementação de sistemas informacionais baseados na visão dos FRBR orientado a objetos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Teoria e prática em catalogação de assunto: a sistematicidade do processo em contexto de bibliotecas universitárias pela perspectiva profissional

A natureza subjetiva da catalogação de assunto imprime certas peculiaridades nas ações dos profissionais que realizam a atividade em domínios específicos. Sendo assim, mostra-se necessária à realização de estudos que cerquem as ações dos catalogadores de assunto, ancoradas na realidade do contexto de bibliotecas universitárias, para a obtenção dos fatores influentes do saber e do fazer profissional. Portanto, analisa-se o conhecimento profissional acerca da sistematicidade do processo da catalogação de assunto em perspectiva sociocognitiva. Para tanto, aplica-se a técnica de Protocolo Verbal em Grupo com três catalogadores de assunto de distintas bibliotecas universitárias do estado de São Paulo, a fim de conhecer a realidade do processo da catalogação de assunto pela perspectiva profissional. Os resultados apontam a necessidade de instrumentos metodológicos que propiciem sustentabilidade prática à catalogação de assunto, em contexto de bibliotecas universitárias.

Diretrizes para diagnóstico morfológico em síndromes mielodisplásicas

As síndromes mielodisplásicas são reconhecidas como doenças que se originam nas células-tronco da medula óssea e que requerem avaliação sistemática e criteriosa de sangue periférico e medula óssea para seu correto diagnóstico. O objetivo deste relato é estabelecer os critérios morfológicos (cito-histológicos) como parâmetros para o diagnóstico de SMD em amostras de sangue periférico e medula óssea, com especial direcionamento aos hematologistas e patologistas clínicos que exercem a hematologia laboratorial na sua rotina de trabalho. Os principais achados morfológicos são listados no final deste relato, na forma de check-list, objetivando a sistematização sobre estes achados.

Fotografia e marketing: uma análise dos atrativos turísticos da cidade de João Pessoa-PB

Photography is a resourse of the most used and effective marketing, besides being an polysemic art, boundless sense; this allows for different readings. Nevertheless, there are those who, through the control and supervision, try to organize its meaning, such as the marketing companies. Others, such as artists, are the responsibility of the observers to give their work some understanding. Resorting a photographic iconography of the tourist attractions of the city of João Pessoa in Brazil from 2005 to 2010. This survey selected images published in catalogs supported by the Fundo de Incentivo a Cultura (FIC) and the Empresa Paraibana de Turismo (PBTUR). The central idea of this qualitative research is the assumption that, in general, tourists crave, even unconsciously, a reality of a tourist attraction motivated by photographic image conveyed the travel market, meanwhile, emphasizes that the art market, the same attraction is exposed and uncovered. Thus it is argued, by photos, such as environmental and sociocultural characteristics are commodified by these organs that have different purposes is not exclusive, given that the FIC supports art and PBTUR sponsors the tourism. Beyond the iconography used for cataloging and photographic analysis, interviews were conducted by the method of visual narratives in ordes to approximate the data with the view of tourists and photographers. In that resulted in a refletion on the current imagistic process involving the release of the tourist destinations in order to be allowed on a critical reading of photographic production and market resources to marketing and promoting the art of the city. It was observed that both the art and photography marketing acquire different symbolic values with respect to their markets promoted by the catalogs analyzed.

Obsessive-compulsive symptoms in patients with panic disorder

Comorbidity studies have shown an important association between panic disorder (PD) and obsessive-compulsive disorder (OCD). The aim of the current study was to evaluate the prevalence of obsessive-compulsive symptoms (OCS) and OCD in patients with PD. Forty-eight consecutive PD cases (DSM-IV diagnostic criteria) referred to a Brazilian university hospital clinic were studied. The Yale Brown Obsessive Compulsive scale (Y-BOCS) checklist was used to identify the OCS. Subclinical OCD was considered when subjects met all but one DSM-IV criteria for OCD (symptoms did not cause significant distress and interference, did not last more than 1 hour per day, or were not considered excessive or irrational), and OCS when only the criterion for presence of obsessions or compulsions was met. Twenty-nine (60.4%) of the 48 patients evaluated (19 men and 29 women) had at least one OCS: nine (18.8%) had mild OCS, 11 (22.9%) had subclinical OCD, and nine (18.8%) had comorbid OCD. Therefore, 41.7% of the patients had either clinical or subclinical OCD. OCS occurred more frequently in women and, in 70.4% of the cases, preceded the onset of PD. Our results suggest that it is important to evaluate systematically the co-occurrence of OCS in patients with PD, due to the considerable overlap found in symptoms, which may have therapeutic implications. As panic symptoms are usually the main complaint, OCS are often found only when directly investigated. (C) 2004 Elsevier B.V. All rights reserved.

Actinopterygii, Siluriformes, Loricariidae, Loricariichthys platymetopon Isbrucker and Nijssen, 1979: first record in Reservoir of Canoas II, Middle Paranapanema River, border of the states of São Paulo and Parana, Brazil.

First record of the cascudo, Loricariichthys platymetopon Isbrucker and Nijssen, 1979 in Canoas II Reservoir (Middle Paranapanema River, border of the states of São Paulo and Parana, Brazil). Its occurrence is due to the flooding of Sete Quedas waterfall, as a result of the construction of Itaipu Reservoir. Furthermore, there is absence or low abundance of natural predators, like Salminus brasiliensis, since this species has difficulties to adapt or even survive in reservoirs.

Ichthyofaunal survey of the Riacho Goulart, tributary of the Tiete River (upper Parana basin).

Fishes assessed in the present study were collected in the Riacho Goulart, a small tributary of Tiete River, upper Parana basin. In order to sample different habitats, four collection sites were selected. A total of 1,241 individuals belonging to 30 species, 15 families, and six orders were captured. The list of fish species presented herein is important for the knowledge of the biodiversity of low Tiete basin.

Reptilia, Squamata, Dipsadidae, Xenodontinae, Taeniophallus brevirostris (Peters, 1863): distribution extension and new state record.

Taeniophallus brevirostris is a dipsadid snake widely distributed in Amazon. Here we report a new Brazilian state record for the species, improving our knowledge of its geographic distribution. 0n 15 May of 2008, one female specimen was collected do Serra do Santo Expediot, Aripuana municipality, state of Mato Grosso, Brazil. This is the first record of this species for the state of Mato Grosso and extends its distribution ca. 300 kilometers from the nearest localities presented in literature.

Squamata, Elapidae, Micrurus surinamensis (Cuvier, 1817): new records and distribution map in the state of Mato Grosso, Brazil, with notes on diet and activity period.

We report six new records of M. surinamensis and present the map of distribution with known records for this species of semiaquatic coral snake in the state of Mato Grosso, Brazil.

Annelida, Hirudinida, Stibarobdella moorei (Oka, 1910): new distribution and host records.

The present report is the northernmost capture of the piscicolid leech Stibarobdella moorei in the western South Atlantic Ocean. This is also the first time S. moorei is found associated to a batoid fish in the Brazilian coast, the eyespot skate Atlantoraja cyclophora. Stibarobdella moorei was found fixed in the dorsal side of a male eyespot skate, caught by bottom trawl around of the São Paulo coast, southeastern Brazil. A brief description of the morphology of the parasite and a discussion on the taxonomic status of the S. moorei are presented.

Biodiversity of brachyuran crabs (Crustacea : Decapoda) from non-consolidated sublittoral bottom on the northern coast of São Paulo State, Brazil

The brachyuran community of the coast of São Paulo State is represented by about 188 species of crabs inhabiting different kinds of coastal marine environments. The biodiversity of brachyurans found on non-consolidated sublittoral bottom was investigated. The Ubatuba region (Ubatumirim, Ubatuba and Mar Virado bays, Couves and Mar Virado Islands, offshore region) was sampled for 3 years (1998-2000), at depths of 2-40 m. All sampling was performed using a fishing boat equipped with two double-rig nets. We collected 79 brachyuran species representing 9 superfamilies (4 Dromioidea, 1 Homoloidea, 2 Calappoidea, 5 Leucosioidea, 20 Majoidea, 7 Parthenopoidea, 17 Portunoidea, 18 Xanthoidea, and 5 Pinnotheroidea) and 41 genera. Ubatuba bay showed the greatest species richness with 50 species, followed by Ubatumirim with 45 and Mar Virado with 29. The number of species collected represents about 57% of the known species of crabs already reported for the shore of São Paulo State. It is worth noticing that this percentage is restricted only to non-consolidated sublittoral bottom. This fact indicates a great biodiversity of the habitat in this studied region, probably one to the diversity of habitat types present in the bays.